For Research Use Only DNMT1 Polyclonal antibody Catalog Number:24206-1-AP Featured Product 43 Publications www.ptglab.com Catalog Number: GenBank Accession Number: Purification Method: Basic Information 24206-1-AP BC126227 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 850 μg/ml by 1786 WB 1:200-1:1000 Nanodrop and 407 μg/ml by Bradford Full Name: IP 0.5-4.0 ug for IP and 1:500-1:1000 method using BSA as the standard; DNA (cytosine-5-)-methyltransferase for WB Source: 1 IHC 1:50-1:500 IF 1:10-1:100 Rabbit Calculated MW: Isotype: 1632 aa, 185 kDa IgG Observed MW: Immunogen Catalog Number: 180-200 kDa AG19116 Applications Tested Applications: Positive Controls: IF, IHC, IP, WB,ELISA WB : HEK-293 cells, C6 cells Cited Applications: IP : HEK-293 cells, CoIP, IF, IHC, WB IHC : human lung cancer tissue, human breast cancer Species Specificity: tissue human, rat Cited Species: IF : HepG2 cells, Chicken, goat, human, pig, rat Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA Background Information methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MV 184 kDa and 145 kDa. (PMID: 10753866) Notable Publications Author Pubmed ID Journal Application Yineng Han 32890413 Oral Dis WB Kalaiselvi Sivalingam 32899583 Brain Sci WB,IF Huihui Zeng 32883320 Respir Res WB Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data IP Result of anti-DNMT1 (IP:24206-1-AP, 5ug; Immunohistochemical analysis of paraffin- HEK-293 cells were subjected to SDS PAGE Detection:24206-1-AP 1:500) with HEK-293 cells embedded human lung cancer tissue slide using followed by western blot with 24206-1-AP (DNMT1 lysate 2500ug. 24206-1-AP (DNMT1 antibody) at dilution of 1:200 Antibody) at dilution of 1:300 incubated at room (under 40x lens). Heat mediated antigen retrieval temperature for 1.5 hours. with Tris-EDTA buffer (pH 9.0). Immunofluorescent analysis of HepG2 cells using 24206-1-AP (DNMT1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG..