DOCSLIB.ORG

Diagnosis of Mycoplasma Mastitis: Validation and Development

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Diagnosis of Mycoplasma Mastitis: Validation and Development DIAGNOSIS OF MYCOPLASMA MASTITIS: VALIDATION AND DEVELOPMENT By SUKOLRAT BOONYAYATRA A dissertation submitted in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY WASHINGTON STATE UNIVERSITY College of Veterinary Medicine DECEMBER 2010 To the Faculty of Washington State University: The members of the Committee appointed to examine the dissertation of SUKOLRAT BOONYAYATRA find it satisfactory and recommend that it be accepted. ______________________________ Lawrence K. Fox, Ph.D., Chair ______________________________ Thomas E. Besser, Ph.D. _____________ ______ ___________ Ashish Sawant, Ph.D. ______________________________ John M. Gay, Ph.D. ii ACKNOWLEDGMENTS I would like to thank Dr. Larry Fox, committee chair, for his guidance, support and encouragement of working with mycoplasma mastitis. Additionally, I am very grateful for his acceptance when I transferred from Tennessee. I thank my other committee members, Dr. John Gay, Dr. Ashish Sawant, and Dr. Tom Besser for their guidance and suggestions on the various aspects and helping me solve many problems with my work. This thesis dissertation would not have been possible without many people who provided technical assistance in the laboratory. I am very thankful to Dorothy Newkirk for her expert assistance. I also thank to other FDIU staffs for their suggestion and helping me go through all lab work smoothly. I want to thank Dr. Ziv Raviv and Dr. Amy Wetzel for their kindness of teaching me everything about real-time PCR when I was in the Ohio State University. I am grateful to Dr. Stephen Oliver, other professers and staffs in the University of Tennessee in Knoxville for providing me a wonderful time in my life when I was in this volunteer state. I want to sincerely thank to the Royal Thai government for the scholarship which allowed me to have this great experience in the United States. I thank my husband, Veerasak Punyapornwithaya, for helping me solve many problems and being such a good husband and friend. I want to thank my daughter, Minnie, for being my biggest motivation of finishing the research. I am deeply grateful to my parents and sisters for their encouragement and understanding for everything I have done. Finally, I would like to thank all people who contributed to the successful of all the work in this thesis dissertation. iii DIAGNOSIS OF MYCOPLASMA MASTITIS: VALIDATION AND DEVELOPMENT Abstract by Sukolrat Boonyayatra, Ph.D. Washington State University December 2010 Chair: Lawrence K. Fox Microbiological culture of milk samples has been used as a standard diagnosis for mycoplasma mastitis. It is suggested to perform with fresh samples for optimum diagnosis. Submission of fresh samples is often difficult given the logistics of collection and shipping of milk samples from farm to laboratory. Therefore , milk samples are usually chilled or frozen before culture. A study of the effects of storage methods on the recovery of Mycoplasma species from milk samples was performed. The results indicated that holding milk at refrigerated temperatures (5°C) for 5 days and freezing milk samples (-20°C) lowers the number of recovered Mycoplasma species . Moreover the addition of glycerol prior freezing to achieve 10% and 30% (v/v) solutions was found to improve the recovery of Mycoplasma species from frozen milk samples. Even though a Mycoplasma -like-colony is observed by a standard culture method, the diagnosis can be misinterpreted as Acholeplasma species which is indistinguishable by culture. To validate and suggest techniques used to discriminate between these two genera, a study on the discrimination between Mycoplasma and Acholeplasma using digitonin and nisin disc diffusion assays, and PCR was performed. Findings revealed a high and comparable efficiency of using iv nisin and digitonin disc diffusion assays and PCR to distinguish Mycoplasma and Acholeplasma species. Given the fastidious nature of Mycoplasma species, and the time-consuming nature of the standard culture method, a diagnostic test of mycoplasma mastitis that is more sensitive, less time consuming, and can speciate mycoplasma mastitis pathogens would be valuable. The development of real-time PCR assays to detect 3 major mycoplasma mastitis pathogens: M. bovis, M. californicum and M. bovigenitalium was investigated to provide an alternative diagnostic tool for mycoplasma mastitis. The results given by the novel real-time PCR assays showed a perfect agreement with the gold standard, and the results were obtained within 4-5 hours. The overall goal of studies reported herein was to investigate improvements in the efficiency of the diagnosis of mycoplasma mastitis in dairy cows. Findings indicate that both phenotypic and genotypic diagnostic techniques can be applied, and in conjunction with current standard techniques, will better identify cows with mycoplasma mastitis. v TABLE OF CONTENTS Page ACKNOWLEDGMENT.................................................................................................... iii ABSTRACT ....................................................................................................................... iv LIST OF TABLES ............................................................................................................. ix LIST OF FIGURES ............................................................................................................ x CHAPTER 1 1.1. INTRODUCTION ............................................................................................1 1.2. REFERENCES .................................................................................................4 CHAPTER 2 (LITERATURE REVIEW) 2.1. MYCOPLASMA SPECIES ................................................................................7 2.2. MYCOPLASMA MASTITIS IN DAIRY CATTLE ........................................8 2.3. EFFECT OF STORAGE METHODS ON SURVIVAL OF MYCOPLASMA 10 2.4. DISCRIMINATION BETWEEN MYCOPLASMA AND ACHOLEPLASMA 12 2.5. DIGITONIN ....................................................................................................13 2.6. NISIN ..............................................................................................................14 2.7. NESTED PCR TECHNIQUE .........................................................................16 2.8. SPECIATION OF MYCOPLASMA SPECIES ...............................................17 2.9. REAL-TIME PCR .........................................................................................19 2.10. CONCLUSION AND HYPOTHESIS ..........................................................23 vi CHAPTER 3 3.1. INTRODUCTION ......................................................................................................44 3.2. MATERIALS AND METHODS ................................................................................46 3.3. RESULTS ...................................................................................................................47 3.4. DISCUSSION .............................................................................................................48 3.5. ACKNOWLEDGMENT.............................................................................................51 3.6. REFERENCES ...........................................................................................................52 CHAPTER 4 4.1. INTRODUCTION ......................................................................................................56 4.2. MATERIALS AND METHODS ................................................................................58 4.3. RESULTS ...................................................................................................................62 4.4. DISCUSSION .............................................................................................................65 4.5. ACKNOWLEDGMENT.............................................................................................68 4.6. REFERENCES ...........................................................................................................68 CHAPTER 5 5.1. INTRODUCTION ......................................................................................................80 5.2. MATERIALS AND METHODS ................................................................................81 5.3. RESULTS ...................................................................................................................87 5.4. DISCUSSION .............................................................................................................89 5.5. ACKNOWLEDGMENT.............................................................................................92 5.6. REFERENCES ...........................................................................................................93 CHAPTER 6 CONCLUSIONS..................................................................................................106 vii APPENDIX A. RAW DATA A.1: Chapter 3 (Refrigeration experiment) ....................................................108 A.2: Chapter3 (Freezing experiment) ............................................................118 A.3: Chapter 4 and 5 ......................................................................................131 B. METHODS B.1: Cloning and filtration techniques for Mycoplasmas .............................143 B.2: DNA extraction and purification using Purelink Genomic DNA kit 144 B.3: PCR to amplify 16S-23S rRNA intergenic spacer region of Mycoplasma species and Acholeplasma
Load more

Recommended publications
  • Bovine Mycoplasmosis
    Bovine mycoplasmosis The microorganisms described within the genus Mycoplasma spp., family Mycoplasmataceae, class Mollicutes, are characterized by the lack of a cell wall and by the little size (0.2-0.5 µm). They are defined as fastidious microorganisms in in vitro cultivation, as they require specific and selective media for the growth, which appears slower if compared to other common bacteria. Mycoplasmas can be detected in several hosts (mammals, avian, reptiles, plants) where they can act as opportunistic agents or as pathogens sensu stricto. Several Mycoplasma species have been described in the bovine sector, which have been detected from different tissues and have been associated to variable kind of gross-pathology lesions. Moreover, likewise in other zoo-technical areas, Mycoplasma spp. infection is related to high morbidity, low mortality and to the chronicization of the disease. Bovine Mycoplasmosis can affect meat and dairy animals of different ages, leading to great economic losses due to the need of prophylactic/metaphylactic measures, therapy and to decreased production. The new holistic approach to multifactorial or chronic diseases of human and veterinary interest has led to a greater attention also to Mycoplasma spp., which despite their elementary characteristics are considered fast-evolving microorganisms. These kind of bacteria, historically considered of secondary importance apart for some species, are assuming nowadays a greater importance in the various zoo-technical fields with increased diagnostic request in veterinary medicine. Also Acholeplasma spp. and Ureaplasma spp. are described as commensal or pathogen in the bovine sector. Because of the phylogenetic similarity and the common metabolic activities, they can grow in the same selective media or can be detected through biomolecular techniques targeting Mycoplasma spp.
    [Show full text]
  • Acholeplasma Florum, a New Species Isolated from Plants? R
    INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Jan. 1984, p. 11-15 Vol. 34, No. 1 0020-7713/84/010011-05$02.OO/O Copyright 0 1984, International Union of Microbiological Societies Acholeplasma florum, a New Species Isolated from Plants? R. E. McCOY,l* H. G. BASHAM,' J. G. TULLY,* D. L. ROSE,2 P. CARLE,3 AND J. M. BOVE3 University of Florida Agricultural Research and Education Center, Fort Lauderdale, Florida 33314'; Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Frederick, Maryland 21 70i2;and lnstitut National de la Recherche Agronomique, Pont de la Maye 33140, France3 Three acholeplasmas isolated from floral surfaces of healthy plants in Florida were found to be similar in their biochemical and serological properties. These organisms did not require serum or cholesterol for growth, although addition of some supplementary fatty acids (as represented by Tween 80) was necessary for growth to occur in serum-free medium. The three strains possessed biochemical properties typical of the Acholeplasmataceae and were distinguished from the nine previously recognized Acholeplasma species by serological and deoxyribopucleic acid-deoxyribonucleic acid hybridization techniques. The genome molec- ular weight of the three Acholeplasma strains was lo9, and the guanine-plus-cytosine content of the deoxyribonucleic acid was 27 to 28 mol%. On the basis of these results and other morphological, biological, and serological properties, we propose that these organisms represent a new species, Acholeplasmaflorurn. Strain L1 (= ATCC 33453) is the type strain. Plant surfaces, particularly flowers, have recently been Media and cultivation procedures. Isolates were routinely proven to be fertile sites for isolation of members of the grown in MC broth or in the serum fraction medium de- Mycoplasrnatales (5, 11-13, 26).
    [Show full text]
  • WO 2018/064165 A2 (.Pdf)
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2018/064165 A2 05 April 2018 (05.04.2018) W !P O PCT (51) International Patent Classification: Published: A61K 35/74 (20 15.0 1) C12N 1/21 (2006 .01) — without international search report and to be republished (21) International Application Number: upon receipt of that report (Rule 48.2(g)) PCT/US2017/053717 — with sequence listing part of description (Rule 5.2(a)) (22) International Filing Date: 27 September 2017 (27.09.2017) (25) Filing Language: English (26) Publication Langi English (30) Priority Data: 62/400,372 27 September 2016 (27.09.2016) US 62/508,885 19 May 2017 (19.05.2017) US 62/557,566 12 September 2017 (12.09.2017) US (71) Applicant: BOARD OF REGENTS, THE UNIVERSI¬ TY OF TEXAS SYSTEM [US/US]; 210 West 7th St., Austin, TX 78701 (US). (72) Inventors: WARGO, Jennifer; 1814 Bissonnet St., Hous ton, TX 77005 (US). GOPALAKRISHNAN, Vanch- eswaran; 7900 Cambridge, Apt. 10-lb, Houston, TX 77054 (US). (74) Agent: BYRD, Marshall, P.; Parker Highlander PLLC, 1120 S. Capital Of Texas Highway, Bldg. One, Suite 200, Austin, TX 78746 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
    [Show full text]
  • Genomic Islands in Mycoplasmas
    G C A T T A C G G C A T genes Review Genomic Islands in Mycoplasmas Christine Citti * , Eric Baranowski * , Emilie Dordet-Frisoni, Marion Faucher and Laurent-Xavier Nouvel Interactions Hôtes-Agents Pathogènes (IHAP), Université de Toulouse, INRAE, ENVT, 31300 Toulouse, France; [email protected] (E.D.-F.); [email protected] (M.F.); [email protected] (L.-X.N.) * Correspondence: [email protected] (C.C.); [email protected] (E.B.) Received: 30 June 2020; Accepted: 20 July 2020; Published: 22 July 2020 Abstract: Bacteria of the Mycoplasma genus are characterized by the lack of a cell-wall, the use of UGA as tryptophan codon instead of a universal stop, and their simplified metabolic pathways. Most of these features are due to the small-size and limited-content of their genomes (580–1840 Kbp; 482–2050 CDS). Yet, the Mycoplasma genus encompasses over 200 species living in close contact with a wide range of animal hosts and man. These include pathogens, pathobionts, or commensals that have retained the full capacity to synthesize DNA, RNA, and all proteins required to sustain a parasitic life-style, with most being able to grow under laboratory conditions without host cells. Over the last 10 years, comparative genome analyses of multiple species and strains unveiled some of the dynamics of mycoplasma genomes. This review summarizes our current knowledge of genomic islands (GIs) found in mycoplasmas, with a focus on pathogenicity islands, integrative and conjugative elements (ICEs), and prophages. Here, we discuss how GIs contribute to the dynamics of mycoplasma genomes and how they participate in the evolution of these minimal organisms.
    [Show full text]
  • Influence of External and Internal Environmental Factors on Intestinal Microbiota of Wild and Domestic Animals A
    Influence of external and internal environmental factors on intestinal microbiota of wild and domestic of animals factors on intestinal microbiota Influence of external and internal environmental Influence of external and internal environmental factors on intestinal microbiota of wild and domestic animals A. Umanets Alexander Umanets Propositions 1. Intestinal microbiota and resistome composition of wild animals are mostly shaped by the animals’ diet and lifestyle. (this thesis) 2. When other environmental factors are controlled, genetics of the host lead to species- or breed specific microbiota patterns. (this thesis) 3. Identifying the response of microbial communities to factors that only have a minor contribution to overall microbiota variation faces the same problems as the discovery of exoplanets. 4. Observational studies in microbial ecology using cultivation- independent methods should be considered only as a guide for further investigations that employ controlled experimental conditions and mechanistic studies of cause-effect relationships. 5. Public fear of genetic engineering and artificial intelligence is not helped by insufficient public education and misleading images created through mass- and social media. 6. Principles of positive (Darwinian) and negative selection govern the repertoire of techniques used within martial arts. Propositions belonging to the thesis, entitled Influence of external and internal environmental factors on intestinal microbiota of wild and domestic animals Alexander Umanets Wageningen, 17 October
    [Show full text]
  • Strains Associated with Oil Palm Lethal Wilt in Colombia
    Page 1 of 37 1 ‘Candidatus Phytoplasma asteris’ strains associated with oil palm lethal wilt in Colombia 2 3 Elizabeth Alvarez , Plant Pathology Program, International Center for Tropical Agriculture 4 (CIAT), Cali, Valle del Cauca, Colombia; Juan F. Mejía , CIAT and Department of 5 Agricultural Sciences and Technologies (DipSA), Alma Mater Studiorum , University of 6 Bologna, Italy; Nicoletta Contaldo and Samanta Paltrinieri , DipSA; Bojan Duduk , 7 Institute of Pesticides and Environmental Protection, Belgrade, Serbia; and Assunta 8 Bertaccini , DipSA. 9 10 Corresponding author: Elizabeth Alvarez 11 Email: [email protected] 12 13 GenBank accession numbers: JX681021, JX681022, JX681023, KF434318, KF434319, 14 KF434320 15 16 All authors have reviewed the manuscript and have approved its submission to the journal 17 Plant Disease . The manuscript is not being submitted elsewhere. 18 19 Plant Disease "First Look" paper • http://dx.doi.org/10.1094/PDIS-12-12-1182-RE posted 10/10/2013 20 This paper has been peer reviewed and accepted for publication but not yet copyedited or proofread. The final published version may differ. Alvarez et al. 1 Plant Disease Page 2 of 37 21 22 Abstract 23 24 Alvarez, E., Mejía, J. F., Contaldo, N., Paltrinieri, S., Duduk, B., and Bertaccini, A. 25 ‘Candidatus Phytoplasma asteris’ strains associated with oil palm lethal wilt in 26 Colombia . Plant Dis. xx: xxx-xxx. 27 28 The distribution of lethal wilt, a severe disease of oil palm, is spreading throughout South 29 America. An incidence of about 30% was recorded in four commercial fields in Colombia. In 30 this study, phytoplasmas were detected in symptomatic oil palms by using specific primers, 31 based on 16S rDNA sequences, in nested polymerase chain reaction assays.
    [Show full text]
  • The Metabolic Pathways of Acholeplasma and Mycoplasma: an Overview
    THE YALE JOURNAL OF BIOLOGY AND MEDICINE 56 (1983), 709-716 The Metabolic Pathways of Acholeplasma and Mycoplasma: An Overview J.D. POLLACK, Ph.D, V.V. TRYON, B.S., AND K.D. BEAMAN, Ph.D. Department ofMedical Microbiology and Immunology, The Ohio State University College of Medicine, Columbus, Ohio Received April 21, 1983 The metabolism of the Mollicutes Acholeplasma and Mycoplasma may be characterized as restricted, for example, by virtue of the apparent absence of cytochrome pigments. Some Mollicutes have lowered ECA values during their logarithmic growth phase, which we speculate may be related to insufficient substrate phosphorylation or insufficient ATP synthesis linked to glycolysis. We found that PEP is carboxylated by preparations of A. laidlawii, but not by other Mollicutes; thus in this organism oxaloacetate from PEP may be a link to other pathways. We found phosphoribosylpyrophosphate in A. laidlawii, which suggests that ribosylation of purines and pyrimidines occurs in Mollicutes other than M. mycoides. The concept that microorganisms have considerable metabolic flexibility is in- grained in the study of biology, and this impression conjures the image of detailed metabolic maps and charts depicting many pathways by which these little engines can metabolize. It is not so certain to us that the class Mollicutes, excluding the Thermoplasma, has this metabolic flexibility. The metabolism of the Mollicutes, to our minds, is becoming, as Lewis Carroll's Alice in Wonderland said, "curiouser and curiouser." We are getting the impression that in Mollicutes catabolism and anabolism are limited; limited by virtue of the absence of, or gaps in, metabolic pathways. As an example, consider the apparent absence of cytochrome pigments, an observation which may serve as one distinguishing feature of the Mollicutes in the microbial world.
    [Show full text]
  • Large-Scale Analysis of the Mycoplasma Bovis Genome Identified Non-Essential, Adhesion- and Virulence-Related Genes
    fmicb-10-02085 September 13, 2019 Time: 16:34 # 1 ORIGINAL RESEARCH published: 13 September 2019 doi: 10.3389/fmicb.2019.02085 Large-Scale Analysis of the Mycoplasma bovis Genome Identified Non-essential, Adhesion- and Virulence-Related Genes Christoph Josi1,2, Sibylle Bürki1, Sara Vidal1, Emilie Dordet-Frisoni3, Christine Citti3, Laurent Falquet4† and Paola Pilo1*† 1 Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland, 2 Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland, 3 UMR 1225, IHAP, Université de Toulouse, INRA, ENVT, Toulouse, France, 4 Department of Biology, Faculty of Science and Medicine, Swiss Institute of Bioinformatics, University of Fribourg, Fribourg, Switzerland Edited by: Mycoplasma bovis is an important pathogen of cattle causing bovine mycoplasmosis. Feng Gao, Tianjin University, China Clinical manifestations are numerous, but pneumonia, mastitis, and arthritis cases are Reviewed by: mainly reported. Currently, no efficient vaccine is available and antibiotic treatments Yong-Qiang He, are not always satisfactory. The design of new, efficient prophylactic and therapeutic Guangxi University, China Angelika Lehner, approaches requires a better understanding of the molecular mechanisms responsible University of Zurich, Switzerland for M. bovis pathogenicity. Random transposon mutagenesis has been widely used in *Correspondence: Mycoplasma species to identify potential gene functions. Such an approach can also be Paola Pilo used to screen genomes and search for essential and non-essential genes for growth. | downloaded: 29.4.2020 [email protected] Here, we generated a random transposon mutant library of M. bovis strain JF4278 †These authors have contributed equally to this work containing approximately 4000 independent insertion sites.
    [Show full text]
  • Downloaded from Genome Website
    bioRxiv preprint doi: https://doi.org/10.1101/2020.11.18.388454; this version posted November 19, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Characterization of the first cultured free-living representative of 2 Candidatus Izimaplasma uncovers its unique biology 3 Rikuan Zheng1,2,3,4, Rui Liu1,2,4, Yeqi Shan1,2,3,4, Ruining Cai1,2,3,4, Ge Liu1,2,4, Chaomin Sun1,2,4* 1 4 CAS Key Laboratory of Experimental Marine Biology & Center of Deep Sea 5 Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China 2 6 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory 7 for Marine Science and Technology, Qingdao, China 3 8 College of Earth Science, University of Chinese Academy of Sciences, Beijing, 9 China 10 4Center of Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China 11 12 * Corresponding author 13 Chaomin Sun Tel.: +86 532 82898857; fax: +86 532 82898857. 14 E-mail address: [email protected] 15 16 17 Key words: Candidatus Izimaplasma, uncultivation, biogeochemical cycling, 18 extracellular DNA, in situ, deep sea 19 Running title: Characterization of the first cultured Izimaplasma 20 21 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.11.18.388454; this version posted November 19, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 22 Abstract 23 Candidatus Izimaplasma, an intermediate in the reductive evolution from Firmicutes 24 to Mollicutes, was proposed to represent a novel class of free-living wall-less bacteria 25 within the phylum Tenericutes found in deep-sea methane seeps.
    [Show full text]
  • A Phylogenetic Analysis of the Mycoplasmas: Basis for Their Lc Assification W
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by DigitalCommons@University of Nebraska University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Public Health Resources Public Health Resources 9-1989 A Phylogenetic Analysis of the Mycoplasmas: Basis for Their lC assification W. G. Weisburg University of Illinois J. G. Tully National Institute of Allergy and Infectious Diseases D. L. Rose National Institute of Allergy and Infectious Diseases J. P. Petzel Iowa State University H. Oyaizu University of Illinois See next page for additional authors Follow this and additional works at: https://digitalcommons.unl.edu/publichealthresources Weisburg, W. G.; Tully, J. G.; Rose, D. L.; Petzel, J. P.; Oyaizu, H.; Yang, D.; Mandelco, L.; Sechrest, J.; Lawrence, T. G.; Van Etten, James L.; Maniloff, J.; and Woese, C. R., "A Phylogenetic Analysis of the Mycoplasmas: Basis for Their lC assification" (1989). Public Health Resources. 310. https://digitalcommons.unl.edu/publichealthresources/310 This Article is brought to you for free and open access by the Public Health Resources at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Public Health Resources by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Authors W. G. Weisburg, J. G. Tully, D. L. Rose, J. P. Petzel, H. Oyaizu, D. Yang, L. Mandelco, J. Sechrest, T. G. Lawrence, James L. Van Etten, J. Maniloff, and C. R. Woese This article is available at DigitalCommons@University of Nebraska - Lincoln: https://digitalcommons.unl.edu/ publichealthresources/310 JOURNAL OF BACTERIOLOGY, Dec. 1989, p. 6455-6467 Vol. 171, No.
    [Show full text]
  • Comparison of Culture and PCR for Detection of Field Isolates of Bovine
    Kafkas Univ Vet Fak Derg Kafkas Universitesi Veteriner Fakultesi Dergisi ISSN: 1300-6045 e-ISSN: 1309-2251 26 (3): 337-342, 2020 Journal Home-Page: http://vetdergikafkas.org Research Article DOI: 10.9775/kvfd.2019.23106 Online Submission: http://submit.vetdergikafkas.org Comparison of Culture and PCR for Detection of Field Isolates of Bovine Milk Mollicutes Abd Al-Bar AL-FARHA 1,a Farhid HEMMATZADEH 2,b Rick TEARLE 3,c Razi JOZANI 4,d Andrew HOARE 5,e Kiro PETROVSKI 6,f 1 Department of Animal Production, Technical Agricultural College, Northern Technical University, Mosul, IRAQ 2 University of Adelaide, School of Animal and Veterinary Sciences, South Australia, AUSTRALIA 3 Davies Centre, The University of Adelaide, School of Animal and Veterinary Sciences, South Australia, AUSTRALIA 4 Tabriz University, Department of Veterinary Clinical Sciences, Tabriz, IRAN 5 South East Vets, South Australia, AUSTRALIA 6 Australian Centre for Antimicrobial Resistance Ecology, The University of Adelaide, School of Animal and Veterinary Sciences, South Australia, AUSTRALIA a ORCID: 0000-0003-1742-6350; b ORCID: 0000-0002-4572-8869; c ORCID: 0000-0003-2243-5091; d ORCID: 0000-0003-2889-4686 e ORCID: 0000-0002-5848-6707; f ORCID: 0000-0003-4016-2576 Article ID: KVFD-2019-23106 Received: 25.07.2019 Accepted: 07.02.2020 Published Online: 07.02.2020 How to Cite This Article Al-Farha AAB, Hemmatzadeh F, Tearle R, Jozani R, Hoare A, PetrovskI K: Comparison of culture and PCR for detection of field isolates of bovine milk mollicutes. Kafkas Univ Vet Fak Derg, 26 (3): 337-342, 2020. DOI: 10.9775/kvfd.2019.23106 Abstract Mycoplasma mastitis raises significant concerns in the dairy industry worldwide.
    [Show full text]
  • Microbial and Mineralogical Characterizations of Soils Collected from the Deep Biosphere of the Former Homestake Gold Mine, South Dakota
    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln US Department of Energy Publications U.S. Department of Energy 2010 Microbial and Mineralogical Characterizations of Soils Collected from the Deep Biosphere of the Former Homestake Gold Mine, South Dakota Gurdeep Rastogi South Dakota School of Mines and Technology Shariff Osman Lawrence Berkeley National Laboratory Ravi K. Kukkadapu Pacific Northwest National Laboratory, [email protected] Mark Engelhard Pacific Northwest National Laboratory Parag A. Vaishampayan California Institute of Technology See next page for additional authors Follow this and additional works at: https://digitalcommons.unl.edu/usdoepub Part of the Bioresource and Agricultural Engineering Commons Rastogi, Gurdeep; Osman, Shariff; Kukkadapu, Ravi K.; Engelhard, Mark; Vaishampayan, Parag A.; Andersen, Gary L.; and Sani, Rajesh K., "Microbial and Mineralogical Characterizations of Soils Collected from the Deep Biosphere of the Former Homestake Gold Mine, South Dakota" (2010). US Department of Energy Publications. 170. https://digitalcommons.unl.edu/usdoepub/170 This Article is brought to you for free and open access by the U.S. Department of Energy at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in US Department of Energy Publications by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Authors Gurdeep Rastogi, Shariff Osman, Ravi K. Kukkadapu, Mark Engelhard, Parag A. Vaishampayan, Gary L. Andersen, and Rajesh K. Sani This article is available at DigitalCommons@University of Nebraska - Lincoln: https://digitalcommons.unl.edu/ usdoepub/170 Microb Ecol (2010) 60:539–550 DOI 10.1007/s00248-010-9657-y SOIL MICROBIOLOGY Microbial and Mineralogical Characterizations of Soils Collected from the Deep Biosphere of the Former Homestake Gold Mine, South Dakota Gurdeep Rastogi & Shariff Osman & Ravi Kukkadapu & Mark Engelhard & Parag A.
    [Show full text]