October 2016 Daniel R. Brown, M.S., Ph.D
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The Role of Earthworm Gut-Associated Microorganisms in the Fate of Prions in Soil
THE ROLE OF EARTHWORM GUT-ASSOCIATED MICROORGANISMS IN THE FATE OF PRIONS IN SOIL Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Taras Jur’evič Nechitaylo aus Krasnodar, Russland 2 Acknowledgement I would like to thank Prof. Dr. Kenneth N. Timmis for his guidance in the work and help. I thank Peter N. Golyshin for patience and strong support on this way. Many thanks to my other colleagues, which also taught me and made the life in the lab and studies easy: Manuel Ferrer, Alex Neef, Angelika Arnscheidt, Olga Golyshina, Tanja Chernikova, Christoph Gertler, Agnes Waliczek, Britta Scheithauer, Julia Sabirova, Oleg Kotsurbenko, and other wonderful labmates. I am also grateful to Michail Yakimov and Vitor Martins dos Santos for useful discussions and suggestions. I am very obliged to my family: my parents and my brother, my parents on low and of course to my wife, which made all of their best to support me. 3 Summary.....................................................………………………………………………... 5 1. Introduction...........................................................................................................……... 7 Prion diseases: early hypotheses...………...………………..........…......…......……….. 7 The basics of the prion concept………………………………………………….……... 8 Putative prion dissemination pathways………………………………………….……... 10 Earthworms: a putative factor of the dissemination of TSE infectivity in soil?.………. 11 Objectives of the study…………………………………………………………………. 16 2. Materials and Methods.............................…......................................................……….. 17 2.1 Sampling and general experimental design..................................................………. 17 2.2 Fluorescence in situ Hybridization (FISH)………..……………………….………. 18 2.2.1 FISH with soil, intestine, and casts samples…………………………….……... 18 Isolation of cells from environmental samples…………………………….………. -
Engineering the Genome of Minimal Bacteria Using CRISPR/Cas9 Tools Iason Tsarmpopoulos
Engineering the genome of minimal bacteria using CRISPR/Cas9 tools Iason Tsarmpopoulos To cite this version: Iason Tsarmpopoulos. Engineering the genome of minimal bacteria using CRISPR/Cas9 tools. Mi- crobiology and Parasitology. Université de Bordeaux, 2017. English. NNT : 2017BORD0787. tel- 01834971 HAL Id: tel-01834971 https://tel.archives-ouvertes.fr/tel-01834971 Submitted on 11 Jul 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THÈSE PRÉSENTÉE POUR OBTENIR LE GRADE DE DOCTEUR DE L’UNIVERSITÉ DE BORDEAUX ÉCOLE DOCTORALE Science de la vie et de la Santé SPÉCIALITÉ Microbiologie and Immunologie Par Iason TSARMPOPOULOS Ingénierie de génome de bactéries minimales par des outils CRISPR/Cas9 Sous la direction de : Monsieur Pascal SIRAND-PUGNET Soutenue le jeudi 07 décembre 2017 à 14h00 Lieu : INRA, 71 avenue Edouard Bourlaux 33882 Villenave d'Ornon salle Amphithéâtre Josy et Colette Bové Membres du jury : Mme Cécile BEBEAR Université de Bordeaux et CHU de Bordeaux Président Mme Florence TARDY Anses-Laboratoire de Lyon Rapporteur M. Matthieu JULES Institut Micalis, INRA and AgroParisTech Rapporteur M. David BIKARD Institut Pasteur Examinateur M. Fabien DARFEUILLE INSERM U1212 - CNRS UMR 5320 Invité Mme Carole LARTIGUE-PRAT INRA - Université de Bordeaux Invité M. -
Role of Protein Phosphorylation in Mycoplasma Pneumoniae
Pathogenicity of a minimal organism: Role of protein phosphorylation in Mycoplasma pneumoniae Dissertation zur Erlangung des mathematisch-naturwissenschaftlichen Doktorgrades „Doctor rerum naturalium“ der Georg-August-Universität Göttingen vorgelegt von Sebastian Schmidl aus Bad Hersfeld Göttingen 2010 Mitglieder des Betreuungsausschusses: Referent: Prof. Dr. Jörg Stülke Koreferent: PD Dr. Michael Hoppert Tag der mündlichen Prüfung: 02.11.2010 “Everything should be made as simple as possible, but not simpler.” (Albert Einstein) Danksagung Zunächst möchte ich mich bei Prof. Dr. Jörg Stülke für die Ermöglichung dieser Doktorarbeit bedanken. Nicht zuletzt durch seine freundliche und engagierte Betreuung hat mir die Zeit viel Freude bereitet. Des Weiteren hat er mir alle Freiheiten zur Verwirklichung meiner eigenen Ideen gelassen, was ich sehr zu schätzen weiß. Für die Übernahme des Korreferates danke ich PD Dr. Michael Hoppert sowie Prof. Dr. Heinz Neumann, PD Dr. Boris Görke, PD Dr. Rolf Daniel und Prof. Dr. Botho Bowien für das Mitwirken im Thesis-Komitee. Der Studienstiftung des deutschen Volkes gilt ein besonderer Dank für die finanzielle Unterstützung dieser Arbeit, durch die es mir unter anderem auch möglich war, an Tagungen in fernen Ländern teilzunehmen. Prof. Dr. Michael Hecker und der Gruppe von Dr. Dörte Becher (Universität Greifswald) danke ich für die freundliche Zusammenarbeit bei der Durchführung von zahlreichen Proteomics-Experimenten. Ein ganz besonderer Dank geht dabei an Katrin Gronau, die mich in die Feinheiten der 2D-Gelelektrophorese eingeführt hat. Außerdem möchte ich mich bei Andreas Otto für die zahlreichen Proteinidentifikationen in den letzten Monaten bedanken. Nicht zu vergessen ist auch meine zweite Außenstelle an der Universität in Barcelona. Dr. Maria Lluch-Senar und Dr. -
Genome Published Outside of SIGS, January – June 2011 Methylovorus Sp
Standards in Genomic Sciences (2011) 4:402-417 DOI:10.4056/sigs.2044675 Genome sequences published outside of Standards in Genomic Sciences, January – June 2011 Oranmiyan W. Nelson1 and George M. Garrity1 1Editorial Office, Standards in Genomic Sciences and Department of Microbiology, Michigan State University, East Lansing, MI, USA The purpose of this table is to provide the community with a citable record of publications of on- going genome sequencing projects that have led to a publication in the scientific literature. While our goal is to make the list complete, there is no guarantee that we may have omitted one or more publications appearing in this time frame. Readers and authors who wish to have publica- tions added to this subsequent versions of this list are invited to provide the bibliometric data for such references to the SIGS editorial office. Phylum Crenarchaeota “Metallosphaera cuprina” Ar-4, sequence accession CP002656 [1] Thermoproteus uzoniensis 768-20, sequence accession CP002590 [2] “Vulcanisaeta moutnovskia” 768-28, sequence accession CP002529 [3] Phylum Euryarchaeota Methanosaeta concilii, sequence accession CP002565 (chromosome), CP002566 (plasmid) [4] Pyrococcus sp. NA2, sequence accession CP002670 [5] Thermococcus barophilus MP, sequence accession CP002372 (chromosome) and CP002373 plasmid) [6] Phylum Chloroflexi Oscillochloris trichoides DG-6, sequence accession ADVR00000000 [7] Phylum Proteobacteria Achromobacter xylosoxidans A8, sequence accession CP002287 (chromosome), CP002288 (plasmid pA81), and CP002289 -
Mycoplasmosis and Upper Respiratory Tract Disease of Tortoises: a Review and Update Elliott R
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector The Veterinary Journal 201 (2014) 257–264 Contents lists available at ScienceDirect The Veterinary Journal journal homepage: www.elsevier.com/locate/tvjl Review Mycoplasmosis and upper respiratory tract disease of tortoises: A review and update Elliott R. Jacobson a,*, Mary B. Brown b, Lori D. Wendland b, Daniel R. Brown b, Paul A. Klein c, Mary M. Christopher d, Kristin H. Berry e a Department of Small Animal Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA b Department of Infectious Disease and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA c Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL 32610, USA d Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA e US Geological Survey, Western Ecological Research Center, Riverside, CA 92518, USA ARTICLE INFO ABSTRACT Article history: Tortoise mycoplasmosis is one of the most extensively characterized infectious diseases of chelonians. Accepted 30 May 2014 A 1989 outbreak of upper respiratory tract disease (URTD) in free-ranging Agassiz’s desert tortoises (Gopherus agassizii) brought together an investigative team of researchers, diagnosticians, pathologists, immunolo- Keywords: gists and clinicians from multiple institutions and -
The Slow Dynamics of Mycoplasma Infections in a Tortoise Host Reveal
Epidemiology and Infection The slow dynamics of mycoplasma infections in a tortoise host reveal heterogeneity cambridge.org/hyg pertinent to pathogen transmission and monitoring Original Paper 1,2 1 3 2 2 Cite this article: Aiello CM, Esque TC, Nussear C. M. Aiello , T. C. Esque , K. E. Nussear , P. G. Emblidge and P. J. Hudson KE, Emblidge PG, Hudson PJ (2019). The slow 1 dynamics of mycoplasma infections in a US Geological Survey, Western Ecological Research Center, Las Vegas Field Station, Henderson, NV 89074, USA; tortoise host reveal heterogeneity pertinent to 2Department of Biology, Center for Infectious Disease Dynamics, Pennsylvania State University, University Park, PA pathogen transmission and monitoring. 16802, USA and 3Department of Geography, University of Nevada, Reno, NV 89557, USA Epidemiology and Infection 147,e12,1–10. https://doi.org/10.1017/S0950268818002613 Abstract Received: 11 April 2018 The epidemiology of infectious diseases depends on many characteristics of disease progres- Revised: 29 June 2018 sion, as well as the consistency of these processes across hosts. Longitudinal studies of infec- Accepted: 25 August 2018 tion can thus inform disease monitoring and management, but can be challenging in wildlife, Key words: particularly for long-lived hosts and persistent infections. Numerous tortoise species of con- Chronic infection; Gopherus agassizii; host servation concern can be infected by pathogenic mycoplasmas that cause a chronic upper resistance; infectiousness; reptile pathogens respiratory tract disease (URTD). Yet, a lack of detailed data describing tortoise responses Author for correspondence: to mycoplasma infections obscures our understanding of URTDs role in host ecology. We C. M. Aiello, E-mail: christinamaiello@gmail. -
Genetic Profiling of Mycoplasma Hyopneumoniae Melissa L
Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 2005 Genetic profiling of Mycoplasma hyopneumoniae Melissa L. Madsen Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Microbiology Commons, Molecular Biology Commons, and the Veterinary Medicine Commons Recommended Citation Madsen, Melissa L., "Genetic profiling of Mycoplasma hyopneumoniae " (2005). Retrospective Theses and Dissertations. 1793. https://lib.dr.iastate.edu/rtd/1793 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. NOTE TO USERS This reproduction is the best copy available. ® UMI Genetic profiling of Mycoplasma hyopneumoniae by Melissa L. Madsen A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Major: Molecular, Cellular and Developmental Biology Program of Study Committee: F. Chris Minion, Major Professor Daniel S. Nettleton Gregory J. Phillips Eileen L. Thacker Eve Wurtele Iowa State University Ames, Iowa 2005 UMI Number: 3200480 INFORMATION TO USERS The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleed-through, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send a complete manuscript and there are missing pages, these will be noted. -
Upper Respiratory Pathogens of Chelonians: a Snotty Turtle
8/21/15 Upper Respiratory Pathogens of Chelonians: A Snotty Turtle Matt Allender, DVM, MS, PhD, Dipl. ACZM University of Illinois Illinois Fall Conference 2015 Pathogens o Ranavirus o Herpes o Mycoplasma General Host Type Species Iridovirus Insects Tipula iridescent virus Chloriridovirus Insects Mosquito iridescent virus Lymphocystivirus Fish Lymphocystivirus disease virus 1 Ranavirus Fish, Amphibians, Reptiles Frog Virus 3 Megalocystivirus Fish Infectious spleen and kidney necrosis virus 1 8/21/15 Ranavirus: Chelonian Significance o Emerging disease in wild and captive chelonia around the world. o Clinical signs include dyspnea, ocular, nasal and oral discharges, and death. 2 8/21/15 Prevalence 20032004 2015 Numerous cases State Species Reference Florida Gopher tortoise Westhouse et al. Florida Box turtle Johnson et al. North Carolina Eastern box turtle DeVoe et al., Allender et al. Tennessee Eastern box turtle Allender et al. Pennsylvania Eastern box turtle Johnson et al. Snapping turtle USGS Maryland Eastern box turtle USGS, Mao? Tortoise Mao? Rhode Island Painted turtle USGS Kentucky Eastern box turtle Ruder et al. Georgia Burmese Star tortoise Johnson et al. New York Eastern Box turtle Johnson et al. Texas Eastern box turtle Johnson et al. Massachusetts Eastern box turtle Allender Virginia Eastern box turtle Allender et al. Indiana Eastern box turtle Johnson pers. comm. Alabama Eastern box turtle Allender et al. Ranavirus: Clinical Signs o Present with sudden onset of severe illness or sudden death with no signs o Rhinitis, conjuctivitis, -
Effectors of Mycoplasmal Virulence 1 2 Virulence Effectors of Pathogenic Mycoplasmas 3 4 Meghan A. May1 And
Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 27 September 2018 doi:10.20944/preprints201809.0533.v1 1 Running title: Effectors of mycoplasmal virulence 2 3 Virulence Effectors of Pathogenic Mycoplasmas 4 5 Meghan A. May1 and Daniel R. Brown2 6 7 1Department of Biomedical Sciences, College of Osteopathic Medicine, University of New 8 England, Biddeford ME, USA; 2Department of Infectious Diseases and Immunology, College 9 of Veterinary Medicine, University of Florida, Gainesville FL, USA 10 11 Corresponding author: 12 Daniel R. Brown 13 Department of Infectious Diseases and Immunology, College of Veterinary Medicine, 14 University of Florida, Gainesville FL, USA 15 Tel: +1 352 294 4004 16 Email: [email protected] 1 © 2018 by the author(s). Distributed under a Creative Commons CC BY license. Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 27 September 2018 doi:10.20944/preprints201809.0533.v1 17 Abstract 18 Members of the genus Mycoplasma and related organisms impose a substantial burden of 19 infectious diseases on humans and animals, but the last comprehensive review of 20 mycoplasmal pathogenicity was published 20 years ago. Post-genomic analyses have now 21 begun to support the discovery and detailed molecular biological characterization of a 22 number of specific mycoplasmal virulence factors. This review covers three categories of 23 defined mycoplasmal virulence effectors: 1) specific macromolecules including the 24 superantigen MAM, the ADP-ribosylating CARDS toxin, sialidase, cytotoxic nucleases, cell- 25 activating diacylated lipopeptides, and phosphocholine-containing glycoglycerolipids; 2) 26 the small molecule effectors hydrogen peroxide, hydrogen sulfide, and ammonia; and 3) 27 several putative mycoplasmal orthologs of virulence effectors documented in other 28 bacteria. -
LCR MSCP Species Accounts, 2008
Lower Colorado River Multi-Species Conservation Program Steering Committee Members Federal Participant Group California Participant Group Bureau of Reclamation California Department of Fish and Game U.S. Fish and Wildlife Service City of Needles National Park Service Coachella Valley Water District Bureau of Land Management Colorado River Board of California Bureau of Indian Affairs Bard Water District Western Area Power Administration Imperial Irrigation District Los Angeles Department of Water and Power Palo Verde Irrigation District Arizona Participant Group San Diego County Water Authority Southern California Edison Company Arizona Department of Water Resources Southern California Public Power Authority Arizona Electric Power Cooperative, Inc. The Metropolitan Water District of Southern Arizona Game and Fish Department California Arizona Power Authority Central Arizona Water Conservation District Cibola Valley Irrigation and Drainage District Nevada Participant Group City of Bullhead City City of Lake Havasu City Colorado River Commission of Nevada City of Mesa Nevada Department of Wildlife City of Somerton Southern Nevada Water Authority City of Yuma Colorado River Commission Power Users Electrical District No. 3, Pinal County, Arizona Basic Water Company Golden Shores Water Conservation District Mohave County Water Authority Mohave Valley Irrigation and Drainage District Native American Participant Group Mohave Water Conservation District North Gila Valley Irrigation and Drainage District Hualapai Tribe Town of Fredonia Colorado River Indian Tribes Town of Thatcher The Cocopah Indian Tribe Town of Wickenburg Salt River Project Agricultural Improvement and Power District Unit “B” Irrigation and Drainage District Conservation Participant Group Wellton-Mohawk Irrigation and Drainage District Yuma County Water Users’ Association Ducks Unlimited Yuma Irrigation District Lower Colorado River RC&D Area, Inc. -
2020 AAZV Proceedings.Pdf
PROCEEDINGS 2020 52ND AMERICAN ASSOCIATION OF ZOO VETERINARIANS Annual Conference 20 September - 24 September 2020 CHARLOTTE KIRK BAER PROCEEDINGS EDITOR CONTINUING EDUCATION Continuing education sponsored by the American College of Zoological Medicine. DISCLAIMER The information appearing in this publication comes exclusively from the authors and contributors identified in each manuscript. The techniques and procedures presented reflect the individual knowledge, experience, and personal views of the authors and contributors. The information presented does not incorporate all known techniques and procedures and is not exclusive. Other procedures, techniques, and technology might also be available. Any questions or requests for additional information concerning any of the manuscripts should be addressed directly to the authors. The sponsoring associations of this conference and resulting publication have not undertaken direct research or formal review to verify the information contained in this publication. Opinions expressed in this publication are those of the authors and contributors and do not necessarily reflect the views of the host associations. The associations are not responsible for errors or for opinions expressed in this publication. The host associations expressly disclaim any warranties or guarantees, expressed or implied, and shall not be liable for damages of any kind in connection with the material, information, techniques, or procedures set forth in this publication. AMERICAN ASSOCIATION OF ZOO VETERINARIANS “Dedicated to wildlife health and conservation” 581705 White Oak Road Yulee, Florida, 32097 904-225-3275 Fax 904-225-3289 Dear Friends and Colleagues, Welcome to our first-ever virtual AAZV Annual Conference! My deepest thanks to the AAZV Scientific Program Committee (SPC) and our other standing Committees for the work they have done to bring us to this point. -
324 Caiman. the Objectives of This Sub
Caiman. The objectives of this Sub-Program are: Caiman. The objectives of this Sub-Program are: • Make the local residents obtain knowledge to be involved direct or indirectly with the Management • Make the local residents obtain knowledge to be involved direct or indirectly with the Management Plan of the Caiman. Plan of the Caiman. • Change of the local population’s attitude, not only with the species subject to harvest, but also with the • Change of the local population’s attitude, not only with the species subject to harvest, but also with the wildlife in general, through the understanding of the importance of the conservation and the sustainable wildlife in general, through the understanding of the importance of the conservation and the sustainable development. development. • Transmit the results of the activities developed inside the management of this species to the local • Transmit the results of the activities developed inside the management of this species to the local communities direct and indirectly involved in the program. communities direct and indirectly involved in the program. Conclusions Conclusions The improvement of the organizational structures of the communities that harvest Caimans will allow to improve the The improvement of the organizational structures of the communities that harvest Caimans will allow to improve the social control on the resource management and to assure a more transparency in the processes, giving the necessary social control on the resource management and to assure a more transparency in the processes, giving the necessary sustainability to the process of harvest of the Caiman. sustainability to the process of harvest of the Caiman.