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The Food Poisoning Toxins of Bacillus Cereus
toxins Review The Food Poisoning Toxins of Bacillus cereus Richard Dietrich 1,†, Nadja Jessberger 1,*,†, Monika Ehling-Schulz 2 , Erwin Märtlbauer 1 and Per Einar Granum 3 1 Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig Maximilian University of Munich, Schönleutnerstr. 8, 85764 Oberschleißheim, Germany; [email protected] (R.D.); [email protected] (E.M.) 2 Department of Pathobiology, Functional Microbiology, Institute of Microbiology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria; [email protected] 3 Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, P.O. Box 5003 NMBU, 1432 Ås, Norway; [email protected] * Correspondence: [email protected] † These authors have contributed equally to this work. Abstract: Bacillus cereus is a ubiquitous soil bacterium responsible for two types of food-associated gastrointestinal diseases. While the emetic type, a food intoxication, manifests in nausea and vomiting, food infections with enteropathogenic strains cause diarrhea and abdominal pain. Causative toxins are the cyclic dodecadepsipeptide cereulide, and the proteinaceous enterotoxins hemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe) and cytotoxin K (CytK), respectively. This review covers the current knowledge on distribution and genetic organization of the toxin genes, as well as mechanisms of enterotoxin gene regulation and toxin secretion. In this context, the exceptionally high variability of toxin production between single strains is highlighted. In addition, the mode of action of the pore-forming enterotoxins and their effect on target cells is described in detail. The main focus of this review are the two tripartite enterotoxin complexes Hbl and Nhe, but the latest findings on cereulide and CytK are also presented, as well as methods for toxin detection, and the contribution of further putative virulence factors to the diarrheal disease. -
Information to Users
INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand corner and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6” x 9" black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. University Microfilms International A Bell & Howell Information Company 3 0 0 North Z eeb Road. Ann Arbor. Ml 4 8106-1346 USA 313/761-4700 800/521-0600 Order Number 9130518 Studies of epidemiology of maize streak virus and itsCicadulina leafhopper vectors in Nigeria Mbey-yame, Asanzi Christopher, Ph.D. -
Performance of Popcorn Introductions for Agronomic Characters, Grain Yield and Popping Qualities in the Forest and Derived Savannah Agro-Ecologies of Nigeria
doi:10.14720/aas.2019.114.1.6 Original research article / izvirni znanstveni članek Performance of popcorn introductions for agronomic characters, grain yield and popping qualities in the forest and derived savannah agro-ecologies of Nigeria Oloruntoba OLAKOJO 1, 2, Gbadebo OLAOYE 1, Adewole AKINTUNDE 3 Received April 25, 2019; accepted August 14, 2019. Delo je prispelo 25. aprila 2019, sprejeto 14. avgusta 2019. Performance of popcorn introductions for agronomic char- Predstavitev uspešnosti uvajanja pokovke na osnovi njenih acters, grain yield and popping qualities in the forest and de- agronomskih lastnosti, pridelka zrnja in kakovosti nabreka- rived savannah agro-ecologies of Nigeria nja v gozdnih in prehodno-savanskih agroekosistemih Nige- Abstract: The study focus on the evaluation of popcorn rije lines for their yield and agronomic potentials. Genetic materi- Izvleček: Raziskava je bile osredotočena na ovredno- als were evaluated under irrigation in a three-replicate in a Ran- tenje linij pokovke glede na njen pridelek in agronomske domized Complete Block Design (RCBD) with a commercial lastnosti. Genetski material je bil ovrednoten ob namakanju variety as check. Two seeds were planted per hole using two- v naključnem bločnem poskusu s tremi ponovitvami v prim- row plots of 5 m long with inter and intra-row spacing of 0.75 m erjavi s komercialno sorto. Po dve semeni sta bili posejani v x 0.5 m, respectively in two locations viz: Ibadan and Ikenne vrstah na ploskvah dolžine 5 m, z medvrstno razdaljo 0,75 m representing the forest and savannah agro-ecologies of Nige- in znotrajvrstno razdaljo 0,5 m, na lokacijah Ibadan in Ikenne, ria respectively. -
Coleomegilla Maculata (Degeer) Predation on Eggs of Colorado Potato Beetle, Leptinotarsa Decemlineata (Say)
University of Massachusetts Amherst ScholarWorks@UMass Amherst Masters Theses 1911 - February 2014 1989 Coleomegilla maculata (DeGeer) predation on eggs of Colorado potato beetle, Leptinotarsa decemlineata (Say). Ruth V. Hazzard University of Massachusetts Amherst Follow this and additional works at: https://scholarworks.umass.edu/theses Hazzard, Ruth V., "Coleomegilla maculata (DeGeer) predation on eggs of Colorado potato beetle, Leptinotarsa decemlineata (Say)." (1989). Masters Theses 1911 - February 2014. 3055. Retrieved from https://scholarworks.umass.edu/theses/3055 This thesis is brought to you for free and open access by ScholarWorks@UMass Amherst. It has been accepted for inclusion in Masters Theses 1911 - February 2014 by an authorized administrator of ScholarWorks@UMass Amherst. For more information, please contact [email protected]. COLEOMEGILLA MACULATA (DEGEER) PREDATION ON EGGS OF COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA (SAY) A Thesis Presented by RUTH V. HAZZARD Submitted to the Graduate School of the University of Massachusetts in partial fulfillment of the requirements of the degree of MASTER OF SCIENCE May 1989 Department of Entomology COLEOMEGILLA MACULATA (DEGEER) PREDATION ON EGGS OF COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA (SAY) A Thesis Presented by RUTH V. HAZZARD Approved as to style and content by: David N. Ferro, Chairperson of Committee Jo^epl/ S. Elkinton, Member ACKNOWLEDGMENTS I am grateful to Dave Ferro for keeping the door to his office always open for me, and thereby opening the door to the profession of Entomology. Special thanks to Roy Van Driesche and Joe Elkinton for their assistance in this work, and to all of my professors for their generosity in teaching. I appreciate the help of Buddy, Tuan and Jennifer in counting eggs and feeding beetles, which made these experiments possible. -
Biosecurity Plan for the Vegetable Industry
Biosecurity Plan for the Vegetable Industry A shared responsibility between government and industry Version 3.0 May 2018 Plant Health AUSTRALIA Location: Level 1 1 Phipps Close DEAKIN ACT 2600 Phone: +61 2 6215 7700 Fax: +61 2 6260 4321 E-mail: [email protected] Visit our web site: www.planthealthaustralia.com.au An electronic copy of this plan is available through the email address listed above. © Plant Health Australia Limited 2018 Copyright in this publication is owned by Plant Health Australia Limited, except when content has been provided by other contributors, in which case copyright may be owned by another person. With the exception of any material protected by a trade mark, this publication is licensed under a Creative Commons Attribution-No Derivs 3.0 Australia licence. Any use of this publication, other than as authorised under this licence or copyright law, is prohibited. http://creativecommons.org/licenses/by-nd/3.0/ - This details the relevant licence conditions, including the full legal code. This licence allows for redistribution, commercial and non-commercial, as long as it is passed along unchanged and in whole, with credit to Plant Health Australia (as below). In referencing this document, the preferred citation is: Plant Health Australia Ltd (2018) Biosecurity Plan for the Vegetable Industry (Version 3.0 – 2018) Plant Health Australia, Canberra, ACT. This project has been funded by Hort Innovation, using the vegetable research and development levy and contributions from the Australian Government. Hort Innovation is the grower-owned, not for profit research and development corporation for Australian horticulture Disclaimer: The material contained in this publication is produced for general information only. -
Identification of the Small Hive Beetle, Aethina Tumida, by Morphological Examination (OIE Method)
Analytical method for animal health REFERENCE: ANSES/SOP/ANA-I1.MOA.1500 - Version 04 February 2020 Identification of the Small Hive Beetle, Aethina tumida, by morphological examination (OIE method) Sophia-Antipolis Laboratory National Reference Laboratory – Bee Health European Union Reference Laboratory – Bee Health This document, in its electronic form, is being made available to users as an analytical method. This document is the property of ANSES. Any reproduction, whether in full or in part, is authorised on the express condition that the source is ANSES/PR3/7/01-07 [version a] mentioned, for example by citing its reference (including its version number and ANSES/FGE/0209 year) and its title. REFERENCE : ANSES/SOP/ANA-I1.MOA.1500 - Version 04 History of the method A method can be updated in order to take changes into account. A change is considered major when it involves the analytic process, the scope or critical points of the analysis method, the application of which may modify the performance characteristics of the method and/or the results. A major change requires major adaptations and either total or partial revalidation. A change is considered minor if it provides useful or practical clarifications, reformulates the text to make it clearer or more accurate, or corrects minor errors. A minor change in the method does not alter its performance characteristics and does not require revalidation. The table below summarises the version history of this method and provides qualifications for the changes. Nature of Version changes Date Main changes (Major / Minor) 1. Reformatting of the method. 2. Updating of references. -
Universidade Estadual De Campinas Instituto De Biologia
UNIVERSIDADE ESTADUAL DE CAMPINAS INSTITUTO DE BIOLOGIA Fabricio José Biasotto Francischini Morphological and molecular characterization of species of Diatraea ssp. (Lepidoptera: Crambidae) and elucidation of dispersal pattern in America continent Caracterização morfológica e molecular de espécies de Diatraea ssp. (Lepidoptera: Crambidae) e elucidação dos padrões de dispersão no continente americano CAMPINAS 2017 Fabricio José Biasotto Francischini Morphological and molecular characterization of species of Diatraea ssp. (Lepidoptera: Crambidae) and elucidation of dispersal pattern in America continent Caracterização morfológica e molecular de espécies de Diatraea ssp. (Lepidoptera: Crambidae) e elucidação dos padrões de dispersão no continente americano Thesis presented to the Institute of Biology of the University of Campinas in partial fulfillment of the requirements for the degree of Doctor in Genetics and Molecular Biology in the area of Plant Genetics and Genetic Breeding Tese apresentada ao Instituto de Biologia da Universidade Estadual de Campinas como parte dos requisitos exigidos para obtenção do título de Doutor em Genética e Biologia Molecular, na Área de Genética Vegetal e Melhoramento Orientadora: Profa. Dra. Maria Imaculada Zucchi Coorientador: Dr. Tederson Galvan ESTE ARQUIVO DIGITAL CORRESPONDE À VERSÃO FINAL DA TESE DEFENDIDA PELO ALUNO FABRICIO JOSÉ BIASOTTO FRANCISCHINI E ORIENTADO PELA PROFa. DRa. MARIA IMACULADA ZUCCHI CAMPINAS 2017 Campinas, 30 de agosto de 2017 COMISSÃO EXAMINADORA Profa. Dra.Maria Imaculada Zucchi (presidente) Prof. Dr. Thiago de Araújo Mastrangelo Prof. Dr. Pedro Takao Yamamoto Dr. Alessandro Alves Pereira Prof. Dr. Alberto Soares Corrêa Os membros da Comissão Examinadora acima assinaram a Ata de defesa, que se encontra no processo de vida acadêmica do aluno. Para Josy Minha eterna esposa Juntos recebemos o dom Da Graça! Constantemente você me ensina a construir nossa família alicerçados em Jesus. -
Tripsacum Dactyloides Scientific Name Tripsacum Dactyloides (L.) L
Tropical Forages Tripsacum dactyloides Scientific name Tripsacum dactyloides (L.) L. Subordinate taxa: Perennial clump grass, Texas, USA Tripsacum dactyloides (L.) L. var. dactyloides Tiller base with short, knotty rhizome and developing prop roots (ILRI 15488) Tripsacum dactyloides (L.) L. var. hispidum (Hitchc.) de Wet & J.R. Harlan Tripsacum dactyloides (L.) L. var. meridonale de Wet & Timothy Tripsacum dactyloides (L.) L. var. mexicanum de Wet & J.R. Harlan Synonyms Single raceme with white stigmas emerging from ♀ spikelets at base of var. dactyloides: basionym Coix dactyloides L.; raceme; purplish stems Tripsacum dactyloides (L.) L. var. occidentale H.C. Single racemes and subdigitate panicle; Cutler & E.S. Anderson anthers emerging from ♂ apical flowers, stigmas on ♀ basal flowers already var. hispidum (Hitchc.) de Wet & J.R. Harlan: senescent Basionym: Tripsacum dactyloides subsp. hispidum Hitchc. Family/tribe Family: Poaceae (alt. Gramineae) subfamily: Panicoideae tribe: Andropogoneae subtribe: Tripsacinae. Morphological description Seed unit with caryopsis Seed units An extremely variable perennial clump grass, with short, fibrous, knotty rhizomes and deep hollow roots. Culms 1‒2.5 (‒4 m) tall, and 3‒5 cm thick at base, branching, prop-rooting from lower nodes; stems purplish, glabrous. Leaf sheath glabrous, often purplish; leaf-blade lanceolate-acuminate, to 30‒75 (‒1.5) cm long and 9‒35 (‒45) mm wide, mostly glabrous, sometimes hairy at the base of the upper blade surface; prominent midrib; Seed production area, Knox margin scabrous; ligule a fringed membrane, 1‒1.5 mm County, Texas, USA (PI 434493) long. Inflorescence 10‒20 (‒30) cm long, terminal and axillary, commonly a single raceme, or subdigitate panicle comprising 2‒3 (‒6) racemes of usually A. -
Aedes Aegypti Mos20 Cells Internalizes Cry Toxins by Endocytosis, and Actin Has a Role in the Defense Against Cry11aa Toxin
Toxins 2014, 6, 464-487; doi:10.3390/toxins6020464 OPEN ACCESS toxins ISSN 2072-6651 www.mdpi.com/journal/toxins Article Aedes aegypti Mos20 Cells Internalizes Cry Toxins by Endocytosis, and Actin Has a Role in the Defense against Cry11Aa Toxin Adriana Vega-Cabrera 1, Angeles Cancino-Rodezno 2, Helena Porta 1 and Liliana Pardo-Lopez 1,* 1 Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo, Postal 510-3, Cuernavaca 62250, Morelos, Mexico; E-Mails: [email protected] (A.V.-C.); [email protected] (H.P.) 2 Facultad de Ciencias, Universidad Nacional Autónoma de México; Av. Universidad 3000, Coyoacán, Distrito Federal 04510, Mexico; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +52-777-3291-624; Fax: +52-777-3291-624. Received: 14 October 2013; in revised form: 11 January 2014 / Accepted: 16 January 2014 / Published: 28 January 2014 Abstract: Bacillus thuringiensis (Bt) Cry toxins are used to control Aedes aegypti, an important vector of dengue fever and yellow fever. Bt Cry toxin forms pores in the gut cells, provoking larvae death by osmotic shock. Little is known, however, about the endocytic and/or degradative cell processes that may counteract the toxin action at low doses. The purpose of this work is to describe the mechanisms of internalization and detoxification of Cry toxins, at low doses, into Mos20 cells from A. aegypti, following endocytotic and cytoskeletal markers or specific chemical inhibitors. Here, we show that both clathrin-dependent and clathrin-independent endocytosis are involved in the internalization into Mos20 cells of Cry11Aa, a toxin specific for Dipteran, and Cry1Ab, a toxin specific for Lepidoptera. -
Contrasting Ladybird Beetle Responses to Urban Environments Across Two US Regions
sustainability Article Context Matters: Contrasting Ladybird Beetle Responses to Urban Environments across Two US Regions Monika Egerer 1,* ID , Kevin Li 2 and Theresa Wei Ying Ong 3,4 ID 1 Environmental Studies Department, University of California, Santa Cruz, Santa Cruz, CA 95064, USA 2 Department of Plant Sciences, University of Göttingen, Göttingen NI 37077, Germany; [email protected] 3 Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA; [email protected] 4 Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08540, USA * Correspondence: [email protected]; Tel.: +1-734-775-8950 Received: 8 April 2018; Accepted: 30 May 2018; Published: 1 June 2018 Abstract: Urban agroecosystems offer an opportunity to investigate the diversity and distribution of organisms that are conserved in city landscapes. This information is not only important for conservation efforts, but also has important implications for sustainable agricultural practices. Associated biodiversity can provide ecosystem services like pollination and pest control, but because organisms may respond differently to the unique environmental filters of specific urban landscapes, it is valuable to compare regions that have different abiotic conditions and urbanization histories. In this study, we compared the abundance and diversity of ladybird beetles within urban gardens in California and Michigan, USA. We asked what species are shared, and what species are unique to urban regions. Moreover, we asked how beetle diversity is influenced by the amount and rate of urbanization surrounding sampled urban gardens. We found that the abundance and diversity of beetles, particularly of unique species, respond in opposite directions to urbanization: ladybirds increased with urbanization in California, but decreased with urbanization in Michigan. -
Downloaded from BOLD Or Requested from Other Authors
www.nature.com/scientificreports OPEN Towards a global DNA barcode reference library for quarantine identifcations of lepidopteran Received: 28 November 2018 Accepted: 5 April 2019 stemborers, with an emphasis on Published: xx xx xxxx sugarcane pests Timothy R. C. Lee 1, Stacey J. Anderson2, Lucy T. T. Tran-Nguyen3, Nader Sallam4, Bruno P. Le Ru5,6, Desmond Conlong7,8, Kevin Powell 9, Andrew Ward10 & Andrew Mitchell1 Lepidopteran stemborers are among the most damaging agricultural pests worldwide, able to reduce crop yields by up to 40%. Sugarcane is the world’s most prolifc crop, and several stemborer species from the families Noctuidae, Tortricidae, Crambidae and Pyralidae attack sugarcane. Australia is currently free of the most damaging stemborers, but biosecurity eforts are hampered by the difculty in morphologically distinguishing stemborer species. Here we assess the utility of DNA barcoding in identifying stemborer pest species. We review the current state of the COI barcode sequence library for sugarcane stemborers, assembling a dataset of 1297 sequences from 64 species. Sequences were from specimens collected and identifed in this study, downloaded from BOLD or requested from other authors. We performed species delimitation analyses to assess species diversity and the efectiveness of barcoding in this group. Seven species exhibited <0.03 K2P interspecifc diversity, indicating that diagnostic barcoding will work well in most of the studied taxa. We identifed 24 instances of identifcation errors in the online database, which has hampered unambiguous stemborer identifcation using barcodes. Instances of very high within-species diversity indicate that nuclear markers (e.g. 18S, 28S) and additional morphological data (genitalia dissection of all lineages) are needed to confrm species boundaries. -
Knockdown of the MAPK P38 Pathway Increases the Susceptibility of Chilo
www.nature.com/scientificreports OPEN Knockdown of the MAPK p38 pathway increases the susceptibility of Chilo suppressalis Received: 07 November 2016 Accepted: 31 January 2017 larvae to Bacillus thuringiensis Published: 06 March 2017 Cry1Ca toxin Lin Qiu1,2, Jinxing Fan2, Lang Liu2, Boyao Zhang2, Xiaoping Wang2, Chaoliang Lei2, Yongjun Lin1 & Weihua Ma1,2 The bacterium Bacillus thuringiensis (Bt) produces a wide range of toxins that are effective against a number of insect pests. Identifying the mechanisms responsible for resistance to Bt toxin will improve both our ability to control important insect pests and our understanding of bacterial toxicology. In this study, we investigated the role of MAPK pathways in resistance against Cry1Ca toxin in Chilo suppressalis, an important lepidopteran pest of rice crops. We first cloned the full-length ofC. suppressalis mitogen-activated protein kinase (MAPK) p38, ERK1, and ERK2, and a partial sequence of JNK (hereafter Csp38, CsERK1, CsERK2 and CsJNK). We could then measure the up-regulation of these MAPK genes in larvae at different times after ingestion of Cry1Ca toxin. Using RNA interference to knockdown Csp38, CsJNK, CsERK1 and CsERK2 showed that only knockdown of Csp38 significantly increased the mortality of larvae to Cry1Ca toxin ingested in either an artificial diet, or after feeding on transgenic rice expressed Cry1Ca. These results suggest that MAPK p38 is responsible for the resistance of C. suppressalis larvae to Bt Cry1Ca toxin. Pore-forming toxins (PFT) play an important role in bacterial pathogenesis and the development of pest resistant strains of crops1–3. Several previous studies have shown that PFTs such as streptolysin O (Streptococcus pyogenes), α -hemolysin (Escherichia coli), α -toxin (Staphylococcus aureus) and Crystal (Cry) toxin (Bacillus thuringien- sis) (Bt) have high toxicity to insect pests4–7.