The Apricot (Prunus Armeniaca L.) Genome Elucidates Rosaceae
Jiang et al. Horticulture Research (2019) 6:128 Horticulture Research https://doi.org/10.1038/s41438-019-0215-6 www.nature.com/hortres ARTICLE Open Access The apricot (Prunus armeniaca L.) genome elucidates Rosaceae evolution and beta-carotenoid synthesis Fengchao Jiang1,2,JunhuanZhang1,2,SenWang3,LiYang1,2, Yingfeng Luo3, Shenghan Gao3, Meiling Zhang1,2, Shuangyang Wu3, Songnian Hu3, Haoyuan Sun1,2 and Yuzhu Wang1,2 Abstract Apricots, scientifically known as Prunus armeniaca L, are drupes that resemble and are closely related to peaches or plums. As one of the top consumed fruits, apricots are widely grown worldwide except in Antarctica. A high-quality reference genome for apricot is still unavailable, which has become a handicap that has dramatically limited the elucidation of the associations of phenotypes with the genetic background, evolutionary diversity, and population diversity in apricot. DNA from P. armeniaca was used to generate a standard, size-selected library with an average DNA fragment size of ~20 kb. The library was run on Sequel SMRT Cells, generating a total of 16.54 Gb of PacBio subreads (N50 = 13.55 kb). The high-quality P. armeniaca reference genome presented here was assembled using long-read single-molecule sequencing at approximately 70× coverage and 171× Illumina reads (40.46 Gb), combined with a genetic map for chromosome scaffolding. The assembled genome size was 221.9 Mb, with a contig NG50 size of 1.02 Mb. Scaffolds covering 92.88% of the assembled genome were anchored on eight chromosomes. Benchmarking Universal Single-Copy Orthologs analysis showed 98.0% complete genes. We predicted 30,436 protein-coding genes, 1234567890():,; 1234567890():,; 1234567890():,; 1234567890():,; and 38.28% of the genome was predicted to be repetitive.
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