(Diptera: Calliphoridae) from India
Total Page：16
File Type：pdf, Size：1020Kb
Load more
Recommended publications
-
Diptera: Calyptratae)
Systematic Entomology (2020), DOI: 10.1111/syen.12443 Protein-encoding ultraconserved elements provide a new phylogenomic perspective of Oestroidea flies (Diptera: Calyptratae) ELIANA BUENAVENTURA1,2 , MICHAEL W. LLOYD2,3,JUAN MANUEL PERILLALÓPEZ4, VANESSA L. GONZÁLEZ2, ARIANNA THOMAS-CABIANCA5 andTORSTEN DIKOW2 1Museum für Naturkunde, Leibniz Institute for Evolution and Biodiversity Science, Berlin, Germany, 2National Museum of Natural History, Smithsonian Institution, Washington, DC, U.S.A., 3The Jackson Laboratory, Bar Harbor, ME, U.S.A., 4Department of Biological Sciences, Wright State University, Dayton, OH, U.S.A. and 5Department of Environmental Science and Natural Resources, University of Alicante, Alicante, Spain Abstract. The diverse superfamily Oestroidea with more than 15 000 known species includes among others blow flies, flesh flies, bot flies and the diverse tachinid flies. Oestroidea exhibit strikingly divergent morphological and ecological traits, but even with a variety of data sources and inferences there is no consensus on the relationships among major Oestroidea lineages. Phylogenomic inferences derived from targeted enrichment of ultraconserved elements or UCEs have emerged as a promising method for resolving difficult phylogenetic problems at varying timescales. To reconstruct phylogenetic relationships among families of Oestroidea, we obtained UCE loci exclusively derived from the transcribed portion of the genome, making them suitable for larger and more integrative phylogenomic studies using other genomic and transcriptomic resources. We analysed datasets containing 37–2077 UCE loci from 98 representatives of all oestroid families (except Ulurumyiidae and Mystacinobiidae) and seven calyptrate outgroups, with a total concatenated aligned length between 10 and 550 Mb. About 35% of the sampled taxa consisted of museum specimens (2–92 years old), of which 85% resulted in successful UCE enrichment. -
Use of Necrophagous Insects As Evidence of Cadaver Relocation
A peer-reviewed version of this preprint was published in PeerJ on 1 August 2017. View the peer-reviewed version (peerj.com/articles/3506), which is the preferred citable publication unless you specifically need to cite this preprint. Charabidze D, Gosselin M, Hedouin V. 2017. Use of necrophagous insects as evidence of cadaver relocation: myth or reality? PeerJ 5:e3506 https://doi.org/10.7717/peerj.3506 Use of necrophagous insects as evidence of cadaver relocation: myth or reality? Damien CHARABIDZE Corresp., 1 , Matthias GOSSELIN 2 , Valéry HEDOUIN 1 1 CHU Lille, EA 7367 - UTML - Unite de Taphonomie Medico-Legale, Univ Lille, 59000 Lille, France 2 Research Institute of Biosciences, Laboratory of Zoology, UMONS - Université de Mons, Mons, Belgium Corresponding Author: Damien CHARABIDZE Email address: [email protected] The use of insects as indicators of postmortem displacement is discussed in many text, courses and TV shows, and several studies addressing this issue have been published. However, the concept is widely cited but poorly understood, and only a few forensic cases have successfully applied such a method. Surprisingly, this question has never be taken into account entirely as a cross-disciplinary theme. The use of necrophagous insects as evidence of cadaver relocation actually involves a wide range of data on their biology: distribution areas, microhabitats, phenology, behavioral ecology and molecular analysis are among the research areas linked to this problem. This article reviews for the first time the current knowledge on these questions and analysze the possibilities/limitations of each method to evaluate their feasibility. This analysis reveals numerous weaknesses and mistaken beliefs but also many concrete possibilities and research opportunities. -
Do Longer Sequences Improve the Accuracy of Identification of Forensically Important Calliphoridae Species?
Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species? Sara Bortolini1, Giorgia Giordani2, Fabiola Tuccia2, Lara Maistrello1 and Stefano Vanin2 1 Department of Life Sciences, University of Modena and Reggio Emilia, Reggio Emilia, Italy 2 School of Applied Sciences, University of Huddersfield, Huddersfield, United Kingdom ABSTRACT Species identification is a crucial step in forensic entomology. In several cases the calculation of the larval age allows the estimation of the minimum Post-Mortem Interval (mPMI). A correct identification of the species is the first step for a correct mPMI estimation. To overcome the difficulties due to the morphological identification especially of the immature stages, a molecular approach can be applied. However, difficulties in separation of closely related species are still an unsolved problem. Sequences of 4 different genes (COI, ND5, EF-1α, PER) of 13 different fly species collected during forensic experiments (Calliphora vicina, Calliphora vomitoria, Lu- cilia sericata, Lucilia illustris, Lucilia caesar, Chrysomya albiceps, Phormia regina, Cyno- mya mortuorum, Sarcophaga sp., Hydrotaea sp., Fannia scalaris, Piophila sp., Megaselia scalaris) were evaluated for their capability to identify correctly the species. Three concatenated sequences were obtained combining the four genes in order to verify if longer sequences increase the probability of a correct identification. The obtained results showed that this rule does not work for the species L. caesar and L. illustris. Future works on other DNA regions are suggested to solve this taxonomic issue. Subjects Entomology, Taxonomy Submitted 19 March 2018 Keywords ND5, COI, PER, Diptera, EF-1α, Maximum-likelihood, Phylogeny Accepted 17 October 2018 Published 17 December 2018 Corresponding author INTRODUCTION Stefano Vanin, [email protected] Species identification is a crucial step in forensic entomology. -
The Blowflies of California
BULLETIN OF THE CALIFORNIA INSECT SURVEY VOLUME 4,NO. 1 THE BLOWFLIES OF CALIFORNIA (Diptera: Calliphoridae) BY MAURICE T. JAMES (Department of Zo'dlogy, State College of Washington, Pullman) UNIVERSITY OF CALIFORNIA PRESS BERKELEY AND LOS ANGELES 1955 BULLETIN OF THE CALIFORNIA INSECT SURVEY Editors: E. G. Linsley, S. B. Freeborn, R. L. Usinger Volume 4, No. 1, pp. 1-34, plates 1-2, 1 figure in text Submitted by Editors, January 13, 1955 Issued October 28, 1955 Price, 50 cents UNIVERSITY OF CALIFORNIA PRESS BERKELEY AND LOS ANGELES CALIFORNIA CAMBRIDGE UNIVERSITY PRESS LONDON. ENGLAND PRINTED BY OFFSET IN THE UNITED STATES OF AMERICA THE BLOWFLIES OF CALIFORNIA (Diptera: Calliphoridae) by Maurice T. James Identification of the blowflies of North America Blowflies are important from a medical and has been made much easier and more secure in veterinary standpoint. Some are obligatory or recent years by the publication of the monograph facultative parasites on man or on domestic or of that family by Hall (1948). However, there other useful animals. In our area, the primary exists ti0 regional treatment that covers any screwworm, Callitroga hominivorax (Coquerel), definite part of the United States. Hall's mono- 'is the only obligatory parasite that invades graph gives only general information about the living tissue, although larvae of Pmtocalliphora, geographical distribution of most of the species. represented in the Califdrnia fauna by seven These considerations, together with the fact that known species, feed on the blood of nesting Hall had obviously examined an insufficient birds, often with fatal results. Of the facultative amount of material from the western states, parasites, Callitroga macellaria (Fabricius), makes a review of the California species partic- Phaenicia sericata (Meigen), and Phormia regina ularly desirable. -
First Record of the Blow Fly Calliphora Grahami from Mexico
First Record of the Blow Fly Calliphora grahami from Mexico Author(s): Carolina Núñez-Vázquez, Jeffery Tomberlin and Oswaldo García-Martínez Source: Southwestern Entomologist, 35(3):313-316. 2010. Published By: Society of Southwestern Entomologists DOI: 10.3958/059.035.0310 URL: http://www.bioone.org/doi/full/10.3958/059.035.0310 BioOne (www.bioone.org) is an electronic aggregator of bioscience research content, and the online home to over 160 journals and books published by not-for-profit societies, associations, museums, institutions, and presses. Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use. Usage of BioOne content is strictly limited to personal, educational, and non-commercial use. Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder. BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research. VOL. 35, NO. 3 SOUTHWESTERN ENTOMOLOGIST SEP. 2010 First Record of the Blow Fly Calliphora grahami1 from Mexico Carolina Núñez-Vázquez2, Jeffery Tomberlin3, and Oswaldo García-Martínez2 Abstract. This is the first record of Calliphora grahami (Aldrich 1930) in Mexico since it was first reported in the United States of America in 1929. Specimens were collected in spring 2008 and 2009 from carcasses of pigs, Sus scrofa L., in a semi- desert area in Saltillo, Coahuila, Mexico. Resumen. Este es el primer registro de Calliphora grahami (Aldrich 1930) en México desde su primer reporte en los Estados Unidos de América en 1929. -
A Global Study of Forensically Significant Calliphorids: Implications for Identification
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by South East Academic Libraries System (SEALS) A global study of forensically significant calliphorids: Implications for identification M.L. Harveya, S. Gaudieria, M.H. Villet and I.R. Dadoura Abstract A proliferation of molecular studies of the forensically significant Calliphoridae in the last decade has seen molecule-based identification of immature and damaged specimens become a routine complement to traditional morphological identification as a preliminary to the accurate estimation of post-mortem intervals (PMI), which depends on the use of species-specific developmental data. Published molecular studies have tended to focus on generating data for geographically localised communities of species of importance, which has limited the consideration of intraspecific variation in species of global distribution. This study used phylogenetic analysis to assess the species status of 27 forensically important calliphorid species based on 1167 base pairs of the COI gene of 119 specimens from 22 countries, and confirmed the utility of the COI gene in identifying most species. The species Lucilia cuprina, Chrysomya megacephala, Ch. saffranea, Ch. albifrontalis and Calliphora stygia were unable to be monophyletically resolved based on these data. Identification of phylogenetically young species will require a faster-evolving molecular marker, but most species could be unambiguously characterised by sampling relatively few conspecific individuals if they were from distant localities. Intraspecific geographical variation was observed within Ch. rufifacies and L. cuprina, and is discussed with reference to unrecognised species. 1. Introduction The advent of DNA-based identification techniques for use in forensic entomology in 1994 [1] saw the beginning of a proliferation of molecular studies into the forensically important Calliphoridae. -
Enhancing Forensic Entomology Applications: Identification and Ecology
Enhancing forensic entomology applications: identification and ecology A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Faculty of Arts and Science TRENT UNIVERSITY Peterborough, Ontario, Canada © Copyright by Sarah Victoria Louise Langer 2017 Environmental and Life Sciences M.Sc. Graduate Program September 2017 ABSTRACT Enhancing forensic entomology applications: identification and ecology Sarah Victoria Louise Langer The purpose of this thesis is to enhance forensic entomology applications through identifications and ecological research with samples collected in collaboration with the OPP and RCMP across Canada. For this, we focus on blow flies (Diptera: Calliphoridae) and present data collected from 2011-2013 from different terrestrial habitats to analyze morphology and species composition. Specifically, these data were used to: 1) enhance and simplify morphological identifications of two commonly caught forensically relevant species; Phormia regina and Protophormia terraenovae, using their frons-width to head- width ratio as an additional identifying feature where we found distinct measurements between species, and 2) to assess habitat specificity for urban and rural landscapes, and the scale of influence on species composition when comparing urban and rural habitats across all locations surveyed where we found an effect of urban habitat on blow fly species composition. These data help refine current forensic entomology applications by adding to the growing knowledge of distinguishing morphological features, and our understanding of habitat use by Canada’s blow fly species which may be used by other researchers or forensic practitioners. Keywords: Calliphoridae, Canada, Forensic Science, Morphology, Cytochrome Oxidase I, Distribution, Urban, Ecology, Entomology, Forensic Entomology ii ACKNOWLEDGEMENTS “Blow flies are among the most familiar of insects. -
Terry Whitworth 3707 96Th ST E, Tacoma, WA 98446
Terry Whitworth 3707 96th ST E, Tacoma, WA 98446 Washington State University E-mail: [email protected] or [email protected] Published in Proceedings of the Entomological Society of Washington Vol. 108 (3), 2006, pp 689–725 Websites blowflies.net and birdblowfly.com KEYS TO THE GENERA AND SPECIES OF BLOW FLIES (DIPTERA: CALLIPHORIDAE) OF AMERICA, NORTH OF MEXICO UPDATES AND EDITS AS OF SPRING 2017 Table of Contents Abstract .......................................................................................................................... 3 Introduction .................................................................................................................... 3 Materials and Methods ................................................................................................... 5 Separating families ....................................................................................................... 10 Key to subfamilies and genera of Calliphoridae ........................................................... 13 See Table 1 for page number for each species Table 1. Species in order they are discussed and comparison of names used in the current paper with names used by Hall (1948). Whitworth (2006) Hall (1948) Page Number Calliphorinae (18 species) .......................................................................................... 16 Bellardia bayeri Onesia townsendi ................................................... 18 Bellardia vulgaris Onesia bisetosa ..................................................... -
Dating Pupae of the Blow Fly Calliphora Vicina Robineau
G C A T T A C G G C A T genes Article Dating Pupae of the Blow Fly Calliphora vicina Robineau–Desvoidy 1830 (Diptera: Calliphoridae) for Post Mortem Interval—Estimation: Validation of Molecular Age Markers Barbara K. Zajac *, Jens Amendt, Marcel A. Verhoff and Richard Zehner Institute of Legal Medicine, Goethe University, 60596 Frankfurt, Germany; [email protected] (J.A.); [email protected] (M.A.V.); [email protected] (R.Z.) * Correspondence: [email protected]; Tel.: +49-163-865-3366 Received: 31 December 2017; Accepted: 5 March 2018; Published: 9 March 2018 Abstract: Determining the age of juvenile blow flies is one of the key tasks of forensic entomology when providing evidence for the minimum post mortem interval. While the age determination of blow fly larvae is well established using morphological parameters, the current study focuses on molecular methods for estimating the age of blow flies during the metamorphosis in the pupal stage, which lasts about half the total juvenile development. It has already been demonstrated in several studies that the intraspecific variance in expression of so far used genes in blow flies is often too high to assign a certain expression level to a distinct age, leading to an inaccurate prediction. To overcome this problem, we previously identified new markers, which show a very sharp age dependent expression course during pupal development of the forensically-important blow fly Calliphora vicina Robineau–Desvoidy 1830 (Diptera: Calliphoridae) by analyzing massive parallel sequencing (MPS) generated transcriptome data. We initially designed and validated two quantitative polymerase chain reaction (qPCR) assays for each of 15 defined pupal ages representing a daily progress during the total pupal development if grown at 17 ◦C. -
The Evolution of Myiasis in Blowflies (Calliphoridae)
International Journal for Parasitology 33 (2003) 1105–1113 www.parasitology-online.com The evolution of myiasis in blowflies (Calliphoridae) Jamie R. Stevens* School of Biological Sciences, University of Exeter, Prince of Wales Road, Exeter EX4 4PS, UK Received 31 March 2003; received in revised form 8 May 2003; accepted 23 May 2003 Abstract Blowflies (Calliphoridae) are characterised by the ability of their larvae to develop in animal flesh. Where the host is a living vertebrate, such parasitism by dipterous larvae is known as myiasis. However, the evolutionary origins of the myiasis habit in the Calliphoridae, a family which includes the blowflies and screwworm flies, remain unclear. Species associated with an ectoparasitic lifestyle can be divided generally into three groups based on their larval feeding habits: saprophagy, facultative ectoparasitism, and obligate parasitism, and it has been proposed that this functional division may reflect the progressive evolution of parasitism in the Calliphoridae. In order to evaluate this hypothesis, phylogenetic analysis of 32 blowfly species displaying a range of forms of ectoparasitism from key subfamilies, i.e. Calliphorinae, Luciliinae, Chrysomyinae, Auchmeromyiinae and Polleniinae, was undertaken using likelihood and parsimony methods. Phylogenies were constructed from the nuclear 28S large subunit ribosomal RNA gene (28S rRNA), sequenced from each of the 32 calliphorid species, together with suitable outgroup taxa, and mitochondrial cytochrome oxidase subunit I and II (COI þ II) sequences, derived primarily from published data. Phylogenies derived from each of the two markers (28S rRNA, COI þ II) were largely (though not completely) congruent, as determined by incongruence-length difference and Kishino-Hasegawa tests. -
Evaluating the Effects of Temperature on Larval Calliphora Vomitoria (Diptera: Calliphoridae) Consumption
Evaluating the effects of temperature on larval Calliphora vomitoria (Diptera: Calliphoridae) consumption Kadeja Evans and Kaleigh Aaron Edited by Steven J. Richardson Abstract: Calliphora vomitoria (Diptera: Calliphoridae) are responsible for more cases of myiasis than any other arthropods. Several species of blowfly, including Cochliomyia hominivorax and Cocholiomya macellaria, parasitize living organisms by feeding on healthy tissues. Medical professionals have taken advantage of myiatic flies, Lucillia sericata, through debridement or maggot therapy in patients with necrotic tissue. This experiment analyzes how temperature influences blue bottle fly, Calliphora vomitoria. consumption of beef liver. After rearing an egg mass into first larval instars, ten maggots were placed into four containers making a total of forty maggots. One container was exposed to a range of temperatures between 18°C and 25°C at varying intervals. The remaining three containers were placed into homemade incubators at constant temperatures of 21°C, 27°C and 33°C respectively. Beef liver was placed into each container and weighed after each group pupation. The mass of liver consumed and the time until pupation was recorded. Three trials revealed that as temperature increased, the average rate of consumption per larva also increased. The larval group maintained at 33°C had the highest consumption with the shortest feeding duration, while the group at 21°C had lower liver consumption in the longest feeding period. Research in this experiment was conducted to understand the optimal temperature at which larval consumption is maximized whether in clinical instances for debridement or in myiasis cases. Keywords: Calliphora vomitoria, Calliphoridae, myiasis, consumption, debridement As an organism begins to decompose, target open wounds or necrotic tissue. -
Forensic Entomology an Introduction
Forensic Entomology An Introduction Dorothy E. Gennard University of Lincoln, UK Forensic Entomology Forensic Entomology An Introduction Dorothy E. Gennard University of Lincoln, UK Copyright © 2007 John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ, England Telephone +44 1243 779777 Email (for orders and customer service enquiries): [email protected] Visit our Home Page on www.wiley.com All Rights Reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, scanning or otherwise, except under the terms of the Copyright, Designs and Patents Act 1988 or under the terms of a licence issued by the Copyright Licensing Agency Ltd, 90 Tottenham Court Road, London W1T 4LP, UK, without the permission in writing of the Publisher. Requests to the Publisher should be addressed to the Permissions Department, John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ, England, or emailed to [email protected], or faxed to (+44) 1243 770620. Designations used by companies to distinguish their products are often claimed as trademarks. All brand names and product names used in this book are trade names, service marks, trademarks or registered trademarks of their respective owners. The Publisher is not associated with any product or vendor mentioned in this book. This publication is designed to provide accurate and authoritative information in regard to the subject matter covered. It is sold on the understanding that the Publisher is not engaged in rendering professional services. If professional advice or other expert assistance is required, the services of a competent professional should be sought.