Curriculum Vitae: Bert Lawrence Semler
Contact Information
Department of Microbiology and Molecular Genetics School of Medicine, Med Sci B237 University of California Irvine, CA 92697 USA E-mail: [email protected] Phone: (949) 824-7573 FAX: (949) 824-2694
Education 1970-1974 University of California, Irvine Bachelor of Science - Biological Sciences 1974-1979 University of California, San Diego Doctor of Philosophy - Biology
Research and Professional Experience
1972-1974 Undergraduate research with Professor H. W. Moore Department of Chemistry, University of California, Irvine
1975-1979 Graduate student with Professor John J. Holland Department of Biology, University of California, San Diego
1979-1983 Postdoctoral fellow with Professor Eckard Wimmer, Department of Microbiology, State University of New York at Stony Brook
1983-1987 Assistant Professor, Department of Microbiology and Molecular Genetics College of Medicine, University of California, Irvine
1987-1991 Associate Professor, Department of Microbiology and Molecular Genetics College of Medicine, University of California, Irvine
1991-2018 Professor, Department of Microbiology and Molecular Genetics School of Medicine, University of California, Irvine
1996-2006 Chair, Department of Microbiology and Molecular Genetics School of Medicine, University of California, Irvine
2000-2010 Associate Director, Center for Virus Research University of California, Irvine
2010-present Director, Center for Virus Research University of California, Irvine
2018-present Distinguished Professor, Department of Microbiology and Molecular Genetics School of Medicine, University of California, Irvine
Bert L. Semler Curriculum Vitae Page 2 Teaching Experience
1973-1974 Teaching assistant - laboratory in organic chemistry Department of Chemistry, University of California, Irvine
1975-1978 Teaching assistant - laboratory in biochemistry, biochemistry lecture course, molecular biology lecture course; Department of Biology University of California, San Diego
1981 Lecturer - Medical Microbiology course for medical students School of Medicine, State University of New York at Stony Brook
1983-present Medical Microbiology course for medical students; School of Medicine University of California, Irvine
1986-1993 Advanced Molecular Virology course for graduate students Department of Microbiology and Molecular Genetics University of California, Irvine
1991-present Viral Gene Expression course for graduate students University of California, Irvine
1997-2000 Eukaryotic Gene Expression course for graduate students University of California, Irvine
Professional Societies
American Society for Microbiology American Society for Virology American Association for the Advancement of Science RNA Society
Journal Editor
2002-2012 Journal of Virology
Editorial Boards
1987-2002 Journal of Virology 1992-present Virology 2013-present Journal of Virology
Scientific Advisory Panels
1988 Ad hoc member, NIH Special Study Section 1989 Guest Reviewer, Scientific Advisory Committee, Damon Runyon-Walter Winchell Cancer Research Fund 1991 Ad hoc member, NIH Special Study Section: Molecularly Targeted Approaches to Antiviral Therapy
Bert L. Semler Curriculum Vitae Page 3 Scientific Advisory Panels (cont.) 1988-1993 Member, University of California Cancer Research Coordinating Committee 1989-1993 Member, NIH Immunology, Virology, and Pathology Study Section 1993-2009 Member, NIH Reviewers Reserve 1995 Ad hoc member, American Cancer Society, Scientific Advisory Committee on Personnel for Research 1995 Ad hoc member, Howard Hughes Medical Institute, Review Panel for Research Training for Medical Students 1996 Ad hoc member, NIH Experimental Virology Study Section 1996-1998 Member, Howard Hughes Medical Institute review panel Postdoctoral Research Fellowships for Physicians program 2000 Ad hoc member, Board of Scientific Counselors Review, NIH Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases 2003 Ad hoc member, NIH Experimental Virology Study Section 2004 Chair, NIH Center for Scientific Review Special Emphasis Panel 2005 Member, NIH Center for Scientific Review Special Emphasis Panel 2006 Ad hoc member, NIH Virology A Study Section 2007 Chair, NIH Center for Scientific Review: Topics in Virology Study Section 2008 Member, NIH Center for Scientific Review Special Emphasis Panel 2008 Ad hoc member, Board of Scientific Counselors Review, NIH Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases 2009 Ad hoc member, NIH Microbiology and Infectious Diseases (MID) Review Committee 2009 Ad hoc member, NIH Virology B Study Section 2010-2013 Member, NIH College of CSR Reviewers 2010 Ad hoc member, NIH Center for Scientific Review: Topics in Infectious Diseases and Microbiology Study Section 2011 Ad hoc reviewer, NIH Director's Pioneer Award Program 2012 Ad hoc member, NIH Center for Scientific Review: Topics in Virology Study Section 2013 Ad hoc reviewer, NIH Director's Pioneer Award Program 2013–2018 Member, NIH Virology B Study Section 2016-2018 Chair, NIH Virology B Study Section Awards and Academic Distinctions 1974-1979 National Institutes of Health Predoctoral Traineeship 1979-1980 American Cancer Society Postdoctoral Fellowship 1980-1983 National Institutes of Health National Research Service Award 1985 American Cancer Society Faculty Research Award Bert L. Semler Curriculum Vitae Page 4
Awards and Academic Distinctions (cont.) 1986-1991 National Institutes of Health Research Career Development Award 1997 Athalie Clark Outstanding Research Award; Research Associates College of Medicine, University of California, Irvine 2001 ISI Highly Cited Researcher 2007 Elected, Fellow of the American Academy of Microbiology 2008 The Bill Joklik Lecturer, 27th Annual Meeting of the American Society for Virology 2010 Senior Fellow of the American Asthma Foundation 2010 Elected, Fellow of the American Association for the Advancement of Science 2011 Elected, President of the American Society for Virology 2013 American Society for Microbiology Distinguished Lecturer
Research Grants - Active Support: 1988-present National Institutes of Health: R01-AI026765, “Impact of host protein dynamics on picornavirus RNA 5’ noncoding region functions,” 4/22/16-3/31/20 (current funding period) 2014-present National Institutes of Health: R01-AI110782, “Role of host cell protein TDP2 as VPg unlinkase during picornavirus replication,” 2/1/14-1/31/20 (current funding period) 2016-present National Institutes of Health: R21-AI126879, “Defining the nuclear vs. cytoplasmic proteome during human rhinovirus infections,” 6/10/16-5/31/19 (current funding period) Training Grant - Active Support: 2002-present National Institutes of Health, “Molecular Biology of Eukaryotic Viruses “9/1/14-8/31/19 (current funding period)
Invited Lectures and Session Chair at Scientific Meetings 1985 IVth Meeting of the European Study Group of the Molecular Biology of Picornaviruses; Seillac, France: Symposium speaker 1986 UCLA Symposium - Proteases in Biological Control and Biotechnology Park City, Utah: Symposium speaker 1988 ICN-UCI International Conference on Virology; Newport Beach, California Symposium speaker 1988 Picornavirus Symposium, Institute of Poliomyelitis and Viral Encephalitides of the USSR Academy of Medical Sciences; Moscow, U.S.S.R. Symposium speaker 1989 Banbury Center Conference on Viral Proteinases; Cold Spring Harbor, New York: Symposium speaker
Bert L. Semler Curriculum Vitae Page 5
Invited Lectures and Session Chair at Scientific Meetings (cont.) 1989 Second International Symposium on Positive Strand RNA Viruses Vienna, Austria: Symposium speaker 1990 The 90th Annual Meeting of the American Society for Microbiology Anaheim, California: Invited speaker and session convenor 1990 The Fifth Rinshoken International Conference - Genetic Determinants of Picornavirus Diseases; Tokyo, Japan: Symposium speaker and session chairperson 1991 Keystone Symposium - Molecular Biology of Human Pathogenic Viruses Lake Tahoe, California: Symposium speaker 1991 Keystone Symposium - Proteolysis in Regulation and Disease; Keystone, Colorado: Symposium speaker 1992 NATO Advanced Study Institute and EEC Course: The Regulation of Gene Expression by Animal Viruses; Mallorca, Spain: Invited speaker and session chair 1994 VIIIth Meeting of the European Study Group on the Molecular Biology of Picornaviruses; Korpilampi, Finland: Symposium speaker 1995 First ICGEB-UCI Virology Symposium; New Delhi, India Invited speaker and session chair 1997 Keystone Symposium - RNA-Protein Interactions; Taos, New Mexico Symposium speaker 1998 Fifth International Symposium on Positive Strand RNA Viruses St. Petersburg, Florida: Symposium speaker 1998 Xth Meeting of the European Study Group on the Molecular Biology of Picornaviruses; Jena, Germany: Symposium speaker 1999 C.N.R.S. Jacques Monod Conference – New Insights into the Mechanism of mRNA Translation: The Significance of RNA Structure; Aussois, France Invited speaker 2001 Gordon Research Conference on Viruses and Cells; Tilton, New Hampshire Session chair 2001 Viruses, Cancer, and the Environment Symposium; Monterrey, Mexico Symposium speaker 2002 Picornavirus Research in 2002: Mini-symposium in honor of Dr. Ellie Ehrenfeld’s 30 years in the field; Bethesda, Maryland: Invited speaker 2002 XIIth Meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPICamerica 2002); Cape Cod, Massachusetts Symposium speaker 2004 Seventh International Symposium on Positive Strand RNA Viruses San Francisco, California: Session chair 2005 XIIIth Meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPIC 2005); Lunteren, The Netherlands Keynote speaker Bert L. Semler Curriculum Vitae Page 6
Invited Lectures and Session Chair at Scientific Meetings (cont.) 2006 XIVth Meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPIC 2006); Saariselkä, Finland: Keynote speaker 2007 International Meeting of the Federation of Korean Microbiological Societies Seoul, Korea: Plenary lecturer 2008 XVth Meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPIC 2008); Sitges, Spain: Keynote speaker 2008 American Society for Virology; 27th Annual Meeting; Ithaca, New York Symposium speaker and session chair 2008 Microbial Pathogens and Their Hosts Symposium; Geneva, Switzerland Symposium speaker 2009 Richard J. Jackson Symposium; University of Cambridge; Cambridge, U.K. Symposium speaker 2009 Gordon Research Conference on Viruses and Cells; Il Ciocco, Italy Symposium speaker 2010 American Asthma Foundation Annual Meeting; San Francisco, California Symposium speaker 2010 XVIth Meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPIC 2010); St. Andrews, Scotland: Plenary speaker 2010 2010 International Symposium on RNA Viruses; Taipei, Taiwan Symposium speaker 2011 Viruses Forever Symposium; Stony Brook, New York Symposium speaker 2011 American Asthma Foundation Annual Meeting; San Francisco, California Symposium speaker 2011 XVth International Congress of Virology; Sapporo, Japan Symposium chair 2012 American Asthma Foundation Annual Meeting; San Francisco, California Symposium speaker 2012 XVIIth Meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPIC 2012); St. Raphael, France: Plenary speaker 2013 South Central Branch and Texas Branch Annual Meeting, American Society for Microbiology; New Orleans, Louisiana: Keynote speaker 2013 8th Congress of Virology; Queretaro, Mexico: Plenary speaker 2014 XVIIIth Meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPIC 2014); Blankenberge, Belgium: Keynote speaker 2014 Instititute for Molecular Virology Symposium; University of Minnesota: Keynote speaker 2014 American Society for Virology; 33rd Annual Meeting; Fort Collins, Colorado Medical Virology Symposium speaker
Bert L. Semler Curriculum Vitae Page 7
Invited Lectures and Session Chair at Scientific Meetings (cont.) 2015 Joint Meeting of the Missouri Valley and Missouri Branches of the American Society for Microbiology; Lincoln, Nebraska: Keynote speaker 2015 Gordon Research Conference on Viruses and Cells; Girona, Spain Discussion leader and symposium speaker 2015 The 13th Igakuken International Symposium on Molecular Basis of Viral Diseases; Tokyo, Japan: Symposium speaker 2016 Host-Pathogen Interactions: Circuits to Systems Symposium; La Jolla, California: Symposium speaker 2016 Viruses Forever II Symposium; Stony Brook, New York: Symposium speaker
Bert L. Semler Curriculum Vitae Page 8 Publications: Peer-reviewed Papers
1. Cajipe, G. J. B., Landen, G., Semler, B. and Moore, H. W. Reaction of aminoquinones and related vinylogous amides with nitrous acid – synthesis and chemistry of cyclic diazo ketones.
J. Org. Chem. 40U :3874U -3878 (1975).
2. Semler, B. L., Hodson, R. C., Williams, S. K. and Howell, S. H. The induction of allophanate lyase during the vegetative cell cycle in light-synchronized cultures of Chlamydomonas
reinhardi. Biochim. Biophys. Acta 399U :71U -78 (1975).
3. Semler, B. L., Perrault, J., Abelson, J. and Holland, J. J. Sequence of a RNA templated by the 3'-OH RNA terminus of defective interfering particles of vesicular stomatitis virus. Proc.
Natl. Acad. Sci. USA 75U :4704U -4708 (1978).
4. Holland, J. J., Grabau, E. A., Jones, C. L. and Semler, B. L. Evolution of multiple genome
mutations during long-term persistent infection by vesicular stomatitis virus. Cell 16U :495U -504 (1979).
5. Semler, B. L., Perrault, J. and Holland, J. J. The nucleotide sequence of the 5' terminus of
vesicular stomatitis virus RNA. Nucleic Acids Res. 6U :3923U -3931 (1979).
6. Semler, B. L. and Holland J. J. Persistent vesicular stomatitis virus infection mediates base
substitutions in viral RNA termini. J. Virol. 32U :420U -428 (1979).
7. Perrault, J. and Semler, B. L. Internal genome deletions in two distinct classes of defective
interfering particles of vesicular stomatitis virus. Proc. Natl. Acad. Sci. USA 76U :6191U -6195 (1979).
8. Rowlands, D., Grabau, E., Spindler, K., Jones, C., Semler, B. and Holland, J. Massive virus protein changes and RNA termini alterations evolving during persistent infection.
Cell 19U :871U -880 (1980).
9. Golini, F., Semler, B. L., Dorner, A. J., and Wimmer, E. Protein-linked RNA of poliovirus is
competent to form an initiation complex of translation in vitro. Nature 287U :600U -603 (1980).
10. Larsen, G. R., Semler, B. L., and Wimmer, E. Stable hairpin structure within the 5'-terminal
85 nucleotides of poliovirus RNA. J. Virol. 37U :328U -335 (1981).
11. Semler, B. L., Anderson, C. W., Kitamura, N., Rothberg, P. G., Wishart, W. L., and Wimmer, E. Poliovirus replication proteins: RNA sequence encoding P3-1b and the sites of proteolytic
processing. Proc. Natl. Acad. Sci. USA 78U :3464U -3468 (1981).
12. Kitamura, N., Semler, B. L., Rothberg, P. G., Larsen, G. R., Adler, C. J., Dorner, A. J., Emini, E. A., Hanecak, R., Lee, J. J., van der Werf, S., Anderson, C. W., and Wimmer, E. Primary
structure, gene organization and polypeptide expression of poliovirus RNA. Nature 291U :547U - 553 (1981).
13. Semler, B. L., Hanecak, R., Anderson, C. W., and Wimmer, E. Cleavage sites in the
polypeptide precursors of poliovirus protein P2-X. Virology 114U :589U -594 (1981).
Bert L. Semler Curriculum Vitae Page 9
14. Larsen, G. R., Anderson, C. W., Dorner, A. J., Semler, B. L., and Wimmer, E. Cleavage sites
within the poliovirus capsid protein precursors. J. Virol. 41U :340U -344 (1982).
15. Semler, B. L., Anderson, C. W., Hanecak, R., Dorner, L. F. and Wimmer, E. A membrane- associated precursor to poliovirus VPg identified by immunoprecipitation with antibodies
directed against a synthetic heptapeptide. Cell 28U :405U -412 (1982).
16. Hanecak, R., Semler, B. L., Anderson, C. W. and Wimmer, E. Proteolytic processing of poliovirus polypeptides: Antibodies to polypeptide P3-7c inhibit cleavage at glutamine-
glycine pairs. Proc. Natl. Acad. Sci. USA 79U :3973U -3977 (1982).
17. Semler, B. L., Hanecak, R., Dorner, L. F., Anderson, C. W. and Wimmer, E. Poliovirus RNA
synthesis in vitro: Structural elements and antibody inhibition. Virology, 126U :624U -635 (1983).
18. Takegami, T., Semler, B. L., Anderson, C. W., and Wimmer, E. Membrane fractions active
in poliovirus RNA replication contain VPg precursor polypeptides. Virology, 128U :33U -47 (1983).
19. Pallansch, M. A., Kew, O. M., Semler, B. L., Omilianowski, D. R., Anderson, C. W., Wimmer,
E., and Rueckert, R. R. The protein processing map of poliovirus. J. Virol. 49U :873U -880 (1984).
20. Dorner, A. J., Semler, B. L., Jackson, R. J., Hanecak, R., Duprey, E., and Wimmer E. In vitro translation of poliovirus RNA: Utilization of internal initiation sites in the reticulocyte lysate.
J. Virol. 50U :507U -514 (1984).
21. Hanecak, R., Semler, B. L., Ariga, H., Anderson, C. W., and Wimmer, E. Expression of a cloned gene segment of poliovirus in E. coli: Evidence for autocatalytic production of the viral
proteinase. Cell 37U :1063U -1073 (1984).
22. Semler, B. L., Dorner, A. J., and Wimmer, E. Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals. Nucleic Acids
Res. 12U :5123U -5141 (1984).
23. Kohara, M., Omata, T., Kameda, A., Semler, B. L., Itoh, H., Wimmer, E., and Nomoto, A. In vitro phenotypic markers of a poliovirus recombinant constructed from infectious cDNA
clones of the neurovirulent Mahoney strain and attenuated Sabin 1 strain. J. Virol. 53U :786U - 792 (1985).
24. Rotbart, H. A., Levin, M. J., Villarreal, L. P., Tracy, S. M., Semler, B. L., and Wimmer, E. Factors affecting the detection of enteroviruses in cerebrospinal fluid with coxsackievirus B3
and poliovirus type 1 cDNA probes. J. Clin. Microbiol. 22U :220U -224 (1985).
25. Omata, T., Kohara, M., Kuge, S., Komatsu, T., Abe, S., Semler, B. L., Kameda, A., Itoh, H., Arita, M., Wimmer, E., and Nomoto, A. Genetic analysis of the attenuation phenotype of
poliovirus type 1. J. Virol. 58U :348U -358 (1986).
26. Kohara, M., Abe, S., Kuge, S., Semler, B. L., Komatsu, T., Arita, M., Itoh, H., and Nomoto, A. An infectious cDNA clone of the poliovirus Sabin strain could be used as a stable repository
and inoculum for the oral polio live vaccine. Virology 151U :21U -30 (1986).
Bert L. Semler Curriculum Vitae Page 10
27. Semler, B. L., Johnson, V. H., and Tracy, S. A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.
Proc. Natl. Acad. Sci. USA. 83U :1777U -1781 (1986).
28. Semler, B. L., Johnson, V. H., Dewalt, P. G., and Ypma-Wong, M. F. Site-specific
mutagenesis of cDNA clones expressing a poliovirus proteinase. J. Cell. Biochem., 33U :39U - 51 (1987).
29. Ypma-Wong, M. F. and Semler B. L. In vitro molecular genetics as a tool for determining the differential cleavage specificities of the poliovirus 3C proteinase. Nucleic Acids
Res. 15U :2069U -2088 (1987).
30. Kuhn, R. J., Wimmer, E., and Semler, B. L. Expression of the poliovirus genome from infectious cDNA is dependent upon arrangements of eukaryotic and prokaryotic sequences
in recombinant plasmids. Virology 157U :560U -564 (1987).
31. Dewalt, P. G. and Semler, B. L. Site directed mutagenesis of proteinase 3C results in a
poliovirus deficient in synthesis of viral RNA polymerase. J. Virol. 61U :2162U -2170 (1987).
32. Ypma-Wong, M. F. and Semler, B. L. Processing determinants required for in vitro cleavage
of the poliovirus P1 precursor to capsid proteins. J. Virol. 61U :3181U -3189 (1987).
33. Johnson, V. H. and Semler, B. L. Defined recombinants of poliovirus and coxsackievirus: sequence-specific deletions and functional substitutions in the 5' non-coding regions of viral
RNAs. Virology 162U :47U -57 (1988).
34. Kuhn, R. J., Tada, H., Ypma-Wong, M. F., Dunn, J. J., Semler, B. L., and Wimmer, E. Construction of a mutagenesis cartridge for poliovirus VPg: Isolation and characterization of
viable and non-viable mutants. Proc. Natl. Acad. Sci. USA 85U :519U -523 (1988).
35. Ypma-Wong, M. F., Dewalt, P. G., Johnson, V. H., Lamb, J. G., and Semler, B. L. Protein 3CD is the major poliovirus proteinase responsible for cleavage of the P1 capsid precursor.
Virology 166U :265U -270 (1988).
36. Kuhn, R. J., Tada, H., Ypma-Wong, M. F., Semler, B. L., and Wimmer, E. Mutational analysis
of the genome-linked protein VPg of poliovirus. J. Virol. 62U :4207U -4215 (1988).
37. Ypma-Wong, M. F., Filman, D. J., Hogle, J. M., and Semler, B. L. Structural domains of the poliovirus polyprotein are major determinants for proteolytic cleavage at Gln-Gly pairs. J.
Biol. Chem. 263U :17846U -17856 (1988).
38. Dildine, S. L. and Semler, B. L. The deletion of 41 proximal nucleotides reverts a poliovirus mutant containing a temperature-sensitive lesion in the 5' noncoding region of genomic RNA.
J. Virol. 63U :847U -862 (1989).
39. Dewalt, P. G., Lawson, M. A., Colonno, R. J., and Semler, B. L. Chimeric picornavirus
polyproteins demonstrate a common 3C proteinase substrate specificity. J. Virol. 63U :3444U - 3452 (1989).
Bert L. Semler Curriculum Vitae Page 11
40. Burns, C. C., Lawson, M. A., Semler, B. L., and Ehrenfeld, E. Effects of mutations in pol poliovirus 3D on RNA polymerase activity and on polyprotein cleavage. J. Virol. 63U :4866U - 4874 (1989).
41. Roos, R. P., Stein, S., Ohara, Y., Fu, J., and Semler, B. L. Infectious cDNA clones of DA
strain of Theiler's murine encephalomyelitis virus. J. Virol. 63U :5492U -5496 (1989).
42. Roos, R. P., Kong, W., and Semler, B. L. Polyprotein processing of Theiler's murine
encephalomyelitis virus. J. Virol. 63U :5344U -5353 (1989).
43. Dewalt, P. G., Blair, W. S., and Semler, B. L. A genetic locus in mutant poliovirus genomes
involved in overproduction of the viral RNA polymerase and 3C proteinase. Virology 174U :504U - 514 (1990).
44. Blair, W. S., Hwang, S.-S., Ypma-Wong, M. F., and Semler, B. L. A mutant poliovirus containing a novel proteolytic cleavage site in VP3 is altered in viral maturation. J.
Virol. 64U :1784U -1793 (1990).
45. Fu, J., Stein, S., Rosenstein, L., Bodwell, T., Routbord, M., Semler, B. L., and Roos, R. P. Neurovirulence determinants of genetically engineered Theiler viruses. Proc. Natl. Acad. Sci.
USA 87U :4125U -4129 (1990).
46. Lawson, M. A., Dasmahapatra, B, and Semler, B. L. Species-specific substrate interaction
of picornavirus 3C proteinase suballelic exchange mutants. J. Biol. Chem. 265U :15920U -15931 (1990).
47. Giachetti, C., and Semler, B. L. Role of a viral membrane polypeptide in strand-specific
initiation of poliovirus RNA synthesis. J. Virol. 65U :2647U -2654 (1991).
48. Dildine, S. L., Stark, K. R., Haller, A. A. and Semler, B. L. Poliovirus translation initiation: differential effects of directed and selected mutations in the 5' noncoding region of viral RNAs.
Virology 182U :742U -752 (1991).
49. Charini, W. A., Burns, C. C., Ehrenfeld, E., and Semler, B. L. Trans rescue of a mutant
poliovirus RNA polymerase function. J. Virol. 65U :2655U -2665 (1991).
50. Blair, W. S., and Semler, B. L. Role for the P4 amino acid residue in substrate utilization by
the poliovirus 3CD proteinase. J. Virol. 65U :6111U -6123 (1991).
51. Lawson, M. A., and Semler, B. L. Poliovirus thiol proteinase 3C can utilize a serine
nucleophile within the putative catalytic triad. Proc. Natl. Acad. Sci. USA. 88U :9919U -9923 (1991).
52. de la Torre, J. C., Giachetti, C., Semler, B. L., and Holland, J. J. High frequency of single- base transitions and extreme frequency of precise multiple-base reversion mutations in
poliovirus. Proc. Natl. Acad. Sci. USA. 89U :2531U -2535 (1992).
53. Dildine, S. L., and Semler, B. L. Conservation of RNA-protein interactions among
picornaviruses. J. Virol. 66U :4364U -4376 (1992).
Bert L. Semler Curriculum Vitae Page 12
54. Haller, A. A., and Semler, B. L. Linker scanning mutagenesis of the internal ribosome entry
site of poliovirus RNA. J. Virol. 66U :5075U -5086 (1992).
55. Lawson, M. A., and Semler, B. L. Alternate poliovirus non-structural protein processing
cascades generated by primary sites of 3C proteinase cleavage. Virology 191U :309U -320 (1992).
56. Giachetti, C., Hwang, S.-S., and Semler, B. L. Cis-acting lesions targeted to the hydrophobic
domain of a poliovirus membrane protein involved in RNA replication. J. Virol. 66U :6045U -6057 (1992).
57. Blair, W. S., Li, X., and Semler, B. L. A cellular cofactor facilitates efficient 3CD cleavage of
the poliovirus P1 precursor. J. Virol. 67U :2336U -2343 (1993).
58. Haller, A. A., Nguyen, J. H. C., and Semler, B. L. Minimum internal ribosome entry site
required for poliovirus infectivity. J. Virol. 67U :7461U -7471 (1993).
59. Charini, W. A., Todd, S., Gutman, G. A., and Semler, B. L. Transduction of a human RNA
sequence by poliovirus. J. Virol. 68U :6547U -6552 (1994).
60. Haller, A. A., and Semler, B. L. Stem-loop structure synergy in binding cellular proteins to
the 5' noncoding region of poliovirus RNA. Virology 206U :923U -934 (1995).
61. Roehl, H. H., and Semler, B. L. Poliovirus infection enhances the formation of two
ribonucleoprotein complexes at the 3' end of viral negative strand RNA. J. Virol. 69U :2954U - 2961 (1995).
62. Todd, S., Nguyen, J. H. C., and Semler, B. L. RNA-protein interactions directed by the 3' end
of human rhinovirus genomic RNA. J. Virol. 69U :3605U -3614 (1995).
63. Blyn, L. B., Chen, R., Semler, B. L., and Ehrenfeld, E. Host cell proteins binding to domain
IV of the 5' noncoding region of poliovirus RNA. J. Virol. 69U :4381U -4389 (1995).
64. Haller, A. A., Stewart, S. R., and Semler, B. L. Attenuation stem-loop lesions in the 5' noncoding region of poliovirus RNA: neuronal cell-specific translation defects. J.
Virol. 70U :1467U -1474 (1996).
65. Blair, W. S., Nguyen, J. H. C., Parsley, T. B., and Semler, B. L. Mutations in the poliovirus 3CD proteinase S1-specificity pocket affect substrate recognition and RNA binding.
Virology 218U :1U -13 (1996).
66. Todd, S., and Semler, B. L. Structure-infectivity analysis of the human rhinovirus genomic
RNA 3' noncoding region. Nucleic Acids Res. 24U :2133U -2142 (1996).
67. Blyn, L. B., Swiderek, K. M., Richards, O. C., Stahl, D. C., Semler, B. L., and Ehrenfeld, E. Poly (rC) binding protein 2 binds to stem-loop IV of the poliovirus RNA 5' noncoding region: Identification by automated LC-tandem mass spectrometry. Proc. Natl. Acad. Sci.
USA 93U :11115U -11120 (1996).
68. Towner, J. S., Ho, T. V., and Semler, B. L. Determinants of membrane association for
poliovirus protein 3AB. J. Biol. Chem. 271U :26810U -26818 (1996). Bert L. Semler Curriculum Vitae Page 13
69. Roehl, H. H., Parsley, T. B., Ho, T. V., and Semler, B. L. Processing of a cellular polypeptide by 3CD proteinase is required for poliovirus ribonucleoprotein complex formation. J.
Virol. 71U :578U -585 (1997).
70. Todd, S., Towner, J. S., and Semler, B. L. Translation and replication properties of the human
rhinovirus genome in vivo and in vitro. Virology 229U :90U -97 (1997).
71. Blyn, L. B., Towner, J. S., Semler, B. L., and Ehrenfeld, E. Requirement of poly (rC) binding
protein 2 for translation of poliovirus RNA. J. Virol. 71U :6243U -6246 (1997).
72. Parsley, T. B., Towner, J. S., Blyn, L. B., Ehrenfeld, E., and Semler, B. L. Poly (rC) binding protein 2 forms a ternary complex with the 5’-terminal sequences of poliovirus RNA and the
viral 3CD proteinase. RNA 3U :1124U -1134 (1997).
73. Todd, S., Towner, J. S., Brown, D. M., and Semler, B. L. Replication-competent
picornaviruses with complete genomic RNA 3’ noncoding region deletions. J. Virol. 71U :8868U - 8874 (1997).
74. Blair, W. S., Parsley, T. B., Bogerd, H. P., Towner, J. S., Semler, B. L., and Cullen, B. R. Utilization of a mammalian cell-based RNA binding assay to characterize the RNA binding
properties of picornavirus 3C proteinases. RNA 4U :215U -225 (1998).
75. Towner, J. S., Mazanet, M. M., and Semler, B. L. Rescue of defective poliovirus RNA
replication by RNA polymerase precursor polyproteins. J. Virol. 72U :7191U -7200 (1998).
76. Negulescu, D., Leong, L. E.-C., Chandy, K. G., Semler, B. L., and Gutman, G. A. Translation initiation of a cardiac voltage-gated potassium channel by internal ribosome entry. J. Biol.
Chem. 273U :20109U -20113 (1998).
77. Stewart, S. R., and Semler, B. L. RNA structure adjacent to the attenuation determinant in
the 5' noncoding region influences poliovirus viability. Nucleic Acids Res. 26U :5318U -5326 (1998).
78. Parsley, T. B., Cornell, C. T., and Semler, B. L. Modulation of the RNA binding and protein processing activities of poliovirus polypeptide 3CD by the viral RNA polymerase domain. J.
Biol. Chem. 274U :12867U -12876 (1999).
79. Bell, Y. C., Semler, B. L., and Ehrenfeld, E. Requirements for RNA replication of a poliovirus
replicon by coxsackievirus RNA polymerase. J. Virol. 73U :9413U -9421 (1999).
80. Stewart, S. R., and Semler, B. L. Pyrimidine-rich region mutations compensate for a stem-
loop V lesion in the 5’ noncoding region of poliovirus genomic RNA. Virology 264U :385U -397 (1999).
81. Walter, B. L., Nguyen, J. H. C., Ehrenfeld, E., and Semler B. L. Differential utilization of poly(rC) binding protein 2 in translation directed by picornavirus IRES elements.
RNA 5U :1570U -1585 (1999).
82. Chapman, N. M., Ragland, A., Leser, J. S., Höfling, K., Willian, S., Semler, B. L., and Tracy, S. A group B coxsackievirus/poliovirus 5’ non-translated region chimera can act as an
attenuated vaccine strain in mice. J. Virol. 74U :4047U -4056 (2000).
Bert L. Semler Curriculum Vitae Page 14
83. Teterina, N. L., Egger, D., Bienz, K., Brown, D. M., Semler, B. L., and Ehrenfeld, E. Requirements for assembly of poliovirus replication complexes and negative-strand RNA
synthesis. J. Virol. 75U :3841U -3850 (2001).
84. Cornell, C. T., and Semler, B. L. Sub-domain specific functions of the RNA polymerase
region of poliovirus 3CD polypeptide. Virology 298U :200U -213 (2002).
85. Walter, B. L., Parsley, T. B., Ehrenfeld, E., and Semler, B. L. Distinct poly(rC) binding protein KH domain determinants for poliovirus translation initiation and viral RNA replication. J.
Virol. 76U :12008U -12022 (2002).
86. Towner, J. S., Brown, D. M., Nguyen, J. H. C., and Semler, B. L. Functional conservation of the hydrophobic domain of polypeptide 3AB between human rhinovirus and poliovirus.
Virology 314U :432U -442 (2003).
87. Brown, D. M., Kauder, S. E., Cornell, C. T., Jang, G. M., Racaniello, V. R., and Semler, B. L. Cell dependent role for the poliovirus 3´ noncoding region in positive-strand RNA synthesis.
J. Virol. 78U :1344U -1351 (2004).
88. Cornell, C. T., Perera, R., Brunner, J. E., and Semler, B. L. Strand-specific RNA synthesis
determinants in the RNA-dependent RNA polymerase of poliovirus. J. Virol. 78U :4397U -4407 (2004).
89. Bedard, K. M., Walter, B.L., and Semler, B. L. Multimerization of poly(rC) binding protein 2
is required for translation initiation mediated by a viral IRES. RNA 10U :1266U -1276 (2004).
90. Jang, G. M., Leong, L. E.-C., Hoang, L. T., Wang, P. H., Gutman, G. A., and Semler, B. L. Structurally distinct elements mediate internal ribosome entry within the 5' noncoding region
of a voltage-gated potassium channel mRNA. J. Biol. Chem. 279U :47419U -47430 (2004).
91. Cornell, C. T., Brunner, J. E., and Semler, B. L. Differential rescue of poliovirus RNA
replication functions by genetically-modified RNA polymerase precursors. J. Virol. 78U :13007U - 13018 (2004).
92. Brunner, J. E., Nguyen, J. H. C., Roehl, H. H., Ho, T. V., Swiderek, K. M., and Semler, B. L. Functional interaction of hnRNP C with poliovirus RNA synthesis initiation complexes. J.
Virol. 79U :3254U -3266 (2005).
93. Kuznetsov, Y. G., Daijogo, S., Zhou, J., Semler, B. L., and McPherson, A. Atomic force
microscopy analysis of icosahedral virus RNA. J. Mol. Biol. 347U :41U -52 (2005).
94. Boerner, J. E., Lyle, J. M., Daijogo, S., Semler, B. L., Schultz, S. C., Kirkegaard, K., and Richards, O. C. Allosteric effects of ligands and mutations on poliovirus RNA-dependent
RNA polymerase. J. Virol. 79U :7803U -7811 (2005).
95. Jimenez, J., Jang, G. M., Semler, B. L., and Waterman, M. L. An internal ribosome entry site
mediates translation of lymphoid enhancer factor-1. RNA 11U :1385U -1399 (2005).
96. Brown, D. M., Cornell, C. T, Tran, G. P., Nguyen, J. H. C., and Semler, B. L. An authentic 3’ noncoding region is necessary for efficient replication of poliovirus transcripts with non-viral
terminal nucleotides. J. Virol. 79U :11962U -11973 (2005).
Bert L. Semler Curriculum Vitae Page 15
97. Bedard, K. M., Daijogo, S., and Semler, B. L. A nucleo-cytoplasmic SR protein functions in
viral IRES-mediated translation initiation. EMBO J. 26U :459U -467 (2007).
98. Perera, R., Daijogo, S., Walter, B. L., Nguyen, J. H. C., and Semler, B. L. Cellular protein
modification by poliovirus: the two faces of poly(rC)-binding protein. J. Virol. 81U :U 8919-8932 (2007).
99. Jang, G. M., Tanaka, B. S., Gutman, G. A., Goldin, A. L., and Semler, B. L. Alternative polyadenylation signals in the 3' noncoding region of a voltage-gated potassium channel
gene are major determinants of mRNA isoform expression. Gene 408U :133U -145 (2008).
100. Sean, P., Nguyen, J. H. C., and Semler, B. L. The linker domain of poly(rC) binding protein
2 is a major determinant in poliovirus cap-independent translation. Virology 378U :243U -253 (2008).
101. Sean, P., Nguyen, J. H. C., and Semler, B. L. Altered interactions between stem-loop IV within the 5' noncoding region of coxsackievirus RNA and poly(rC) binding protein 2: effects
on IRES-mediated translation and viral infectivity. Virology 389U :45U -58 (2009).
102. Brunner, J. E., Ertel, K. J., Rozovics, J. M., and Semler, B. L. Delayed kinetics of poliovirus RNA synthesis in a human cell line with reduced levels of hnRNP C proteins.
Virology 400U :240U -247 (2010).
103. Ertel, K. J., Brunner, J. E., and Semler, B. L. Mechanistic consequences of hnRNP C binding
to both RNA termini of poliovirus negative-strand RNA intermediates. J. Virol. 84U :4229U -4242 (2010).
104. Grainger, L., Cicchini, L., Rak, M., Petrucelli, A., Fitzgerald, K. D., Semler, B. L., and Goodrum, F. Stress-inducible alternative translation initiation of human cytomegalovirus
latency protein pUL138. J. Virol. 84U :9472U -9486 (2010).
105. Huang, C., Lokugamage, K. G., Rozovics, J. M., Narayanan, K., Semler, B. L., and Makino, S. Alphacoronavirus transmissible gastroenteritis virus nsp1 protein suppresses protein translation in mammalian cells and in cell-free HeLa extracts but not in rabbit reticulocyte
lysate. J. Virol. 85U :638U -643 (2011).
106. Rozovics, J. M., Virgen-Slane, R., and Semler, B. L. Engineered picornavirus VPg-RNA
substrates: analysis of a tyrosyl-RNA phosphodiesterase activity. PLoS ONE 6U :U e16559 (2011).
107. Fitzgerald, K. D., and Semler, B. L. Re-localization of cellular protein SRp20 during poliovirus infection: bridging a viral IRES to the host cell translation apparatus. PLoS
Pathog. 7U :U e1002127 (2011).
108. Huang, C., Lokugamage, K. G., Rozovics, J. M., Narayanan, K., Semler, B. L., and Makino, S. SARS coronavirus nsp1 protein induces template-dependent endonucleolytic cleavage of mRNAs: viral mRNAs are resistant to nsp1-induced RNA cleavage. PLoS Pathog.
7U :e1002433U (2011).
109. Virgen-Slane, R., Rozovics, J. M., Fitzgerald, K. D., Ngo, T., Chou, W., van der Heden van Noort, G. J., Filippov, D. V., Gershon, P. D., and Semler, B. L. An RNA virus hijacks an incognito function of a DNA repair enzyme. Proc. Natl. Acad. Sci. USA 109:14634-14639 (2012). Bert L. Semler Curriculum Vitae Page 16 110. Rozovics, J. M., Chase, A. J., Cathcart, A. L., Chou, W., Gershon, P. D., Palusa, S., Wilusz, J., and Semler, B. L. Picornavirus modification of a host mRNA decay protein. mBio 3(6):e00431-12 (2012).
111. Feng, Q., Hato, S. V., Langereis, M. A., Zoll, J., Virgen-Slane, R., Peisley, A., Hur, S., Semler, B. L., van Rij, R. P., and van Kuppeveld, F. J. M. MDA5 detects the double- stranded RNA replicative form in picornavirus-infected cells. Cell Rep.2:1187-1196 (2012).
112. Fitzgerald, K. D., Chase, A. J., Cathcart, A. L., Tran, G. P., and Semler, B. L. Viral proteinase requirements for the nucleo-cytoplasmic re-localization of cellular splicing factor SRp20 during picornavirus infections. J. Virol. 87:2390-2400 (2013).
113. Fitzgerald, K. D., and Semler, B. L. Poliovirus infection induces the co-localization of cellular protein SRp20 with TIA-1, a cytoplasmic stress granule protein. Virus Res. 176:223-231 (2013).
114. Cathcart, A. L., Rozovics, J. M., and Semler, B. L. Cellular mRNA decay protein AUF1 negatively regulates enterovirus and human rhinovirus infections. J. Virol. 87:10423-10434 (2013).
115. Chase, A. J., and Semler, B. L. Differential cleavage of IRES trans-acting factors (ITAFs) in cells infected by human rhinovirus. Virology 449:35-44 (2014).
116. Langereis, M. A., Feng, Q., Nelissen, F., Virgen-Slane, R., van der Heden van Noort, G. J., Maciejewski, S., Filippov, D. V., Semler, B. L., van Delft, F., and van Kuppeveld, F. J. Modification of picornavirus genomic RNA using ‘click’ chemistry shows that unlinking of the VPg peptide is dispensable for translation and replication of the incoming viral RNA. Nucleic Acids Res. 42:2473-2482 (2014).
117. Chase, A. J., Daijogo, S., and Semler, B. L. Inhibition of poliovirus-induced cleavage of cellular protein PCBP2 reduces the levels of viral RNA replication. J. Virol. 88:3192-3201 (2014).
118. Cathcart, A. L., and Semler, B. L. Differential restriction patterns of mRNA decay factor AUF1 during picornavirus infections. J. Gen. Virol. 95:1488-1492 (2014).
119. Tsai, B. P., Jimenez, J., Lim, S., Fitzgerald, K. D., Zhang, M., Chuah, C. T., Axelrod, H., Wilson, L., Ong, S. T., Semler, B. L., and Waterman, M. L. A novel Bcr-Abl-mTOR-eIF4A axis regulates IRES-mediated translation of LEF-1. Open Biol. 4:140180 (2014).
120. Hopcraft, S. E., Azarm, K. D., Israelow, B., Lévêque, N., Schwarz, M. C., Hsu, T.-H., Chambers, M. T., Sourisseau, M., Semler, B. L., and Evans, M. Viral determinants of miR- 122-independent hepatitis C virus replication. mSphere 1(1): e00009-15 (2015).
121. Maciejewski, S., Nguyen, J. H. C., Gómez-Herreros, F., Cortés-Ledesma, F., Caldecott, K. W., and Semler, B. L. Divergent requirement for a DNA repair enzyme during enterovirus infections. mBio 7(1):e01931-15 (2015).
122. Wehbe, M., Huguenin, A., Leveque, N., Semler, B. L., Hamze, M., Andreoletti, L., and Bouin, A. Construction of a subgenomic CV-B3 replicon expressing emerald green fluorescent protein to assess viral replication of a cardiotropic enterovirus strain in cultured human cells. J. Virol. Methods 230:1-8 (2016). Bert L. Semler Curriculum Vitae Page 17
123. Flather, D., Cathcart, A. L., Cruz, C., Baggs, E., Ngo, T., Gershon, P. D., and Semler, B. L. Generation of recombinant polioviruses harboring RNA affinity tags in the 5′ and 3′ noncoding regions of genomic RNAs. Viruses 8(2):39 (2016).
124. Lévêque, N., Garcia, M., Bouin, A., Nguyen, J. H. C., Tran, G. P., Andreoletti, L., and Semler, B. L. Functional consequences of RNA 5' terminal deletions on coxsackievirus B3 RNA replication and ribonucleoprotein complex formation. J. Virol. 91(16): e00423-17 (2017).
125. Maciejewski, S., Ullmer, W., and Semler, B. L. VPg unlinkase/TDP2 in cardiovirus infected cells: re-localization and proteolytic cleavage. Virology 516:139-146 (2018).
126. Ullmer, W. and Semler, B. L. Direct and indirect effects on viral translation and RNA replication are required for AUF1 restriction of enterovirus infections in human cells. mBio 9(5):e01669-18 (2018).
127. Flather, D., Nguyen, H. C., Semler, B. L., and Gershon, P.D. Exploitation of nuclear functions by human rhinovirus, a cytoplasmic RNA virus. PLoS Pathog. 14:e1007277 (2018).
128. Bouin, A., Gretteau, P. A., Wehbe, M., Renois, F., N’guyen, Y., Lévêque, N., Vu, M., Tracy, S., Chapman, N. M., Bruneval, P., Fornes, P., Semler, B. L., and Andreoletti, L. Enterovirus persistence in cardiac cells of patients suffering from idiopathic dilated cardiomyopathy is linked to 5' terminal genomic RNA-deleted viral populations with viral-encoded proteinase activities. Circulation, in press (2019).
Publications: Books, Book Chapters, and Invited Reviews 1. Holland, J. J., Villarreal, L. P., Breindl, M., Semler, B. L. and D. Kohne. Defective interfering
particles attenuate virus lethality in vivo and in vitro. In AnimalU Virology U - D. Baltimore, A. Huang and C. F. Fox, eds., IV. 773-786, Academic Press, New York (1976).
2. Perrault, J., Semler, B. L., Leavitt, R. W. and Holland J. J. Inverted complementary sequences in defective interfering particle RNAs of vesicular stomatitis virus and their
possible role in autointerference. p. 527-538. In: NegativeU Strand Viruses and the Host Cell U - B. W. J. Mahy and R. D. Barry, eds., Academic Press, New York (1978).
3. Holland, J. J., Semler, B. L., Jones, C., Perrault, J., Reid, L. and Roux, L. Role of DI, virus mutation and host response in persistent infections by envelope RNA viruses, p. 57-73.
In: PersistentU Viruses U - J. Stevens, G. Todaro and C. F. Fox, eds., Academic Press, New York (1978).
4. Holland, J. J., Kennedy, S. I. T., Semler, B. L., Jones, C. L., Roux, L. and Grabau, E. A. Defective interfering RNA viruses and the host-cell response, pp. 137-192. In: H. Fraenkel-
Conrat and R. R. Wagner, eds., ComprehensiveU Virology,U Vol. 16, Plenum Press, New York (1980).
5. Adler, C. J., Semler, B. L., Rothberg, P. G., Kitamura, N., and Wimmer, E. Structure, possible function and biosynthesis of VPg, the genome-linked protein of poliovirus, pp. 356-371. In:
H. Holzer, ed., InternationalU Symposia on Metabolic Conversion of Enzymes,U Springer Verlag, Berlin, Heidelberg and New York (1981).
Bert L. Semler Curriculum Vitae Page 18
6. Diamond, D. C., Hanecak, R., Semler, B. L., Jameson, B. A., Bonin, J., and Wimmer, E. Structure, proteolytic processing, and neutralization antigenic sites of poliovirus, pp. 193-201.
In: J. S. Schell and P. Starlinger, eds., TheU Impact of Gene Transfer Techniques in Eukaryotic
Cell Biology,U Springer-Verlarg, Berlin, Heidelberg (1985)
7. Omata, T., Kohara, M., Abe, S., Itoh, H., Komatsu, T., Arita, M., Semler, B. L., Wimmer, E., Kuge, S., Kameda, A., and Nomoto, A. Construction of recombinant viruses between Mahoney and Sabin strains of type 1 poliovirus and their biological characteristics, p. 279-
283. In: R. A. Lerner, R. M. Chanock and F. Brown, eds. VaccinesU U 85, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1985).
8. Nomoto, A., Kohara, M., Kuge, S., Kawamura, N., Arita, M., Kamatsu, T., Abe, S., Semler, B. L., Wimmer, E., and Itoh, H. Study on virulence of poliovirus type 1 using in vitro modified viruses, pp. 437-452. In: M. A. Brinton and R. R. Rueckert,
eds, PositiveU U StrandU U RNAU U VirusesU ,U Alan R. Liss, Inc., New York (1987).
9. Nomoto, A., Kohara, M., Kuge, S., Abe, S., Semler, B. L., Komatsu, T., Arita, M., and Itoh, H. The development of new poliovirus vaccines based on molecular cloning, pp. 43-54. In: E.
Kurstak, R. G. Marusyk, F. A. Murphy, and M. H. V. VanRegenmortel, eds., AppliedU Virology
Research,U Vol. 1: New Vaccines and Chemotherapy, Plenum Publishing Company, New York (1988).
10. Semler, B. L., Kuhn, R. J., and Wimmer, E. Replication of the poliovirus genome, pp. 23-48.
In: E. Domingo, J. Holland, and P. Ahlquist, eds., RNAU Genetics,U Vol. I. CRC Press, Inc., Boca Raton, Florida (1988).
11. Dewalt, P. G. and Semler, B. L. Molecular biology and genetics of poliovirus protein
processing, pp. 73-93. In: B. L. Semler and E. Ehrenfeld, eds. MolecularU Aspects of
Picornavirus Infection and Detection. U American Society for Microbiology, Washington, D.C. (1989).
12. Semler, B. L. and Ehrenfeld, E. (eds.). MolecularU Aspects of Picornavirus Infection and
Detection.U American Society for Microbiology, Washington, D. C. (1989).
13. Giachetti, C., and Semler, B. L. Molecular genetic analysis of poliovirus RNA replication by mutagenesis of a VPg precursor polypeptide, pp. 83-93. In: M. A. Brinton and F. X. Heinz,
eds. NewU Aspects of Positive-Strand RNA Viruses.U American Society for Microbiology, Washington, D. C. (1990).
14. Lawson, M. A., and Semler, B. L. Picornavirus protein processing: Enzymes, substrates,
and genetic regulation. Curr. Top. Microbiol. Immunol. 161U :49U -87 (1990).
15. Roos, R. P., Fu, J., Kong, W., Stein, S., Bodwell, T., Rosenstein, L., Routbort, M., and Semler, B. L. Infectious Theiler's virus cDNA clones: Studies of intratypic recombinants and viral
polyprotein processing, pp. 128-134. In: M. A. Brinton and F. X. Heinz, eds. NewU Aspects
of Positive-Strand RNA Viruses.U American Society for Microbiology, Washington, D. C. (1990).
16. Blair, W. S., and Semler, B. L. Self-cleaving proteases. Curr. Opin. Cell Biol. 3U :1039U -1045 (1991).
Bert L. Semler Curriculum Vitae Page 19
17. Blair, W. S., Li, X., and Semler, B. L. 3CD cleavage of the poliovirus P1 precursor: a model for complex proteinase/substrate interactions, pp. 225-244. In: L. Carrasco, N. Sonenberg,
and E. Wimmer, eds. RegulationU of Gene Expression in Animal Viruses.U Plenum Press, London (1993).
18. Dougherty, W. G., and Semler, B. L. Expression of virus-encoded proteinases: functional
and structural similarities with cellular enzymes. Microbiol. Rev. 57U :781U -822 (1993).
19. Haller, A. A., and Semler, B. L. Enteroviruses, pp. 481-491. In: R. R. McKendall and W. G.
Stroop, eds. HandbookU of Neurovirology.U Marcel Dekker, Inc., New York, (1994).
20. Roehl, H. H., and Semler, B. L. In vitro biochemical methods for investigating RNA-protein
interactions in picornaviruses, pp. 169-182. In: K. W. Adolph, ed. MethodsU in Molecular
Genetics, Vol. 4.U Academic Press, Inc., Orlando, Florida (1994).
21. Haller, A. A., and Semler, B. L. Translation and host cell shutoff, pp. 113-133. In: H. A.
Rotbart, ed. HumanU Enterovirus Infections.U American Society for Microbiology, Washington, D.C. (1995).
22. Ehrenfeld, E., and Semler, B. L. Anatomy of the poliovirus internal ribosome entry site. Curr.
Top. Microbiol. Immunol. 203U :65U -83 (1995).
23. Todd, S., and Semler, B. L. The molecular biology of poliovirus, pp. 13-25. In: R. A. Meyers,
ed. EncyclopediaU of Molecular Biology and Molecular Medicine, U Vol. 5. VCH Publishers, Weinheim, Germany (1996).
24. Towner, J. S., Roehl, H. H., Todd, S., Parsley, T. B., Ho, T. V., and Semler, B. L. Complex formation and template recognition in poliovirus RNA replication, pp. 3-22. In: S. Jameel
and E. K. Wagner, eds. CurrentU Developments in Animal Virology.U Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, India and Science Publishers Inc., Lebanon, N. H., USA (1996).
25. Stewart, S. R., and Semler, B. L. RNA determinants of picornavirus cap-independent
translation initiation. Seminars in Virology 8U :242U -255 (1997).
26. Leong, L. E.-C., Cornell, C. T., and Semler, B. L. Processing determinants and functions of cleavage products of picornavirus polyproteins, pp. 187-197. In: B. L. Semler and E.
Wimmer, eds. MolecularU Biology of Picornaviruses,U American Society for Microbiology Press, Washington, D.C. (2002).
27. Semler, B. L., and Wimmer, E., (eds.) MolecularU Biology of Picornaviruses,U American Society for Microbiology Press, Washington, D.C. (2002).
28. Cornell, C. T., and Semler, B. L. Gene expression and replication of picornaviruses, pp. 93-
117. In: R. A. Meyers, ed. EncyclopediaU of Molecular Cell Biology and Molecular Medicine,U 2nd Edition. Wiley-VCH Publishers, Weinheim, Germany (2004).
29. Bedard, K. M., and Semler, B. L. Regulation of picornavirus gene expression. Microbes
Infect. 6U :702U -713 (2004).
30. Semler, B. L. Poliovirus proves IRES-istible in vivo. J. Clin. Invest. 113U :1678U -1681 (2004).
Bert L. Semler Curriculum Vitae Page 20
31. Semler, B. L. Resistance is futile. Nat. Genet. 37U :665U -666 (2005).
32. Brunner, J. E., and Semler, B. L. Positive-strand RNA virus replication in vertebrate hosts,
pp. 135-146. In: B. Mahy and V. ter Muelen, eds. TopleyU and Wilson’s Microbiology and th Microbial Infections,U 10 edition, Virology Volume. Edward Arnold (Publishers) Limited, London, UK (2005).
33. Semler, B. L., and Ertel, K. J. Picornaviridae: molecular biology, pp. 129-140. In: B. Mahy rd and M. Van Regenmortel, eds. EncyclopediaU of Virology,U 3 edition, vol. 4. Elsevier Ltd., Oxford, U.K. (2008).
34. Sean, P., and Semler, B. L. Coxsackievirus B RNA replication: lessons from poliovirus. Curr.
Top. Microbiol. Immunol. 323U :89U -121 (2008).
35. Semler, B. L., and Waterman, M. L. IRES-mediated pathways to polysomes: nuclear versus
cytoplasmic routes. Trends Microbiol. 16U :U 1-5 (2008).
36. Fitzgerald, K. D., and Semler, B. L. Bridging IRES elements in mRNAs to the eukaryotic
translation apparatus. Biochim. Biophys. Acta 1789U :518U -528 (2009).
37. Rozovics, J. M., and Semler, B. L. Genome replication I: the players, pp. 107-125. In: E.
Ehrenfeld, E. Domingo, and R. Roos, eds. TheU Picornaviruses,U American Society for Microbiology Press, Washington, D.C. (2010).
38. Kirkegaard, K., and Semler, B. L. Genome replication II: the process, pp. 127-140. In: E.
Ehrenfeld, E. Domingo, and R. Roos, eds. TheU Picornaviruses,U American Society for Microbiology Press, Washington, D.C. (2010).
39. Daijogo, S., and Semler, B. L. Mechanistic intersections between picornavirus translation
and RNA replication. Adv Virus Res. 80U :U 1-24 (2011).
40. Chase, A. J., and Semler, B. L. Viral subversion of host functions for picornavirus translation
and RNA replication. Future Virol. 7U :179U -191 (2012).
41. Du, X., Semler, B. L., and Gomez, C. M. Revelations from a bicistronic calcium channel gene. Cell Cycle 13:875-876 (2014).
42. Cathcart, A. L., Baggs, E. L., and Semler, B. L. Picornaviruses: pathogenesis and molecular biology. Reference Module in Biomedical Research (3rd ed.), Elsevier (2014).
43. Lévêque, N., and Semler, B. L. A 21st century perspective of poliovirus replication. PLoS Pathog. 11:e1004825 (2015).
44. Flather, D., and Semler, B. L. Picornaviruses and nuclear functions: targeting a cellular compartment distinct from the replication site of a positive-strand RNA virus. Front. Microbiol. 6:594 (2015).
45. Ullmer, W. and Semler, B. L. Diverse strategies used by picornaviruses to escape host RNA decay pathways. Viruses 8:335 (2016).
46. Karginov, T. A., Pastor, D. P., Semler, B. L., and Gomez, C. M. Mammalian polycistronic mRNAs and disease. Trends Genet. 33:129-142 (2017).
Bert L. Semler Curriculum Vitae Page 21
47. Maciejewski, S., and Semler, B. L. Hijacking host functions for translation and RNA replication by enteroviruses, pp. 23-49. In: W. Jackson and C. Coyne, eds. Enteroviruses: Omics, Molecular Biology, and Control, Caister Academic Press; Poole, U.K. (2018).