DOCSLIB.ORG

Bert Lawrence Semler

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Bert Lawrence Semler Curriculum Vitae: Bert Lawrence Semler Contact Information Department of Microbiology and Molecular Genetics School of Medicine, Med Sci B237 University of California Irvine, CA 92697 USA E-mail: [email protected] Phone: (949) 824-7573 FAX: (949) 824-2694 Education 1970-1974 University of California, Irvine Bachelor of Science - Biological Sciences 1974-1979 University of California, San Diego Doctor of Philosophy - Biology Research and Professional Experience 1972-1974 Undergraduate research with Professor H. W. Moore Department of Chemistry, University of California, Irvine 1975-1979 Graduate student with Professor John J. Holland Department of Biology, University of California, San Diego 1979-1983 Postdoctoral fellow with Professor Eckard Wimmer, Department of Microbiology, State University of New York at Stony Brook 1983-1987 Assistant Professor, Department of Microbiology and Molecular Genetics College of Medicine, University of California, Irvine 1987-1991 Associate Professor, Department of Microbiology and Molecular Genetics College of Medicine, University of California, Irvine 1991-2018 Professor, Department of Microbiology and Molecular Genetics School of Medicine, University of California, Irvine 1996-2006 Chair, Department of Microbiology and Molecular Genetics School of Medicine, University of California, Irvine 2000-2010 Associate Director, Center for Virus Research University of California, Irvine 2010-present Director, Center for Virus Research University of California, Irvine 2018-present Distinguished Professor, Department of Microbiology and Molecular Genetics School of Medicine, University of California, Irvine Bert L. Semler Curriculum Vitae Page 2 Teaching Experience 1973-1974 Teaching assistant - laboratory in organic chemistry Department of Chemistry, University of California, Irvine 1975-1978 Teaching assistant - laboratory in biochemistry, biochemistry lecture course, molecular biology lecture course; Department of Biology University of California, San Diego 1981 Lecturer - Medical Microbiology course for medical students School of Medicine, State University of New York at Stony Brook 1983-present Medical Microbiology course for medical students; School of Medicine University of California, Irvine 1986-1993 Advanced Molecular Virology course for graduate students Department of Microbiology and Molecular Genetics University of California, Irvine 1991-present Viral Gene Expression course for graduate students University of California, Irvine 1997-2000 Eukaryotic Gene Expression course for graduate students University of California, Irvine Professional Societies American Society for Microbiology American Society for Virology American Association for the Advancement of Science RNA Society Journal Editor 2002-2012 Journal of Virology Editorial Boards 1987-2002 Journal of Virology 1992-present Virology 2013-present Journal of Virology Scientific Advisory Panels 1988 Ad hoc member, NIH Special Study Section 1989 Guest Reviewer, Scientific Advisory Committee, Damon Runyon-Walter Winchell Cancer Research Fund 1991 Ad hoc member, NIH Special Study Section: Molecularly Targeted Approaches to Antiviral Therapy Bert L. Semler Curriculum Vitae Page 3 Scientific Advisory Panels (cont.) 1988-1993 Member, University of California Cancer Research Coordinating Committee 1989-1993 Member, NIH Immunology, Virology, and Pathology Study Section 1993-2009 Member, NIH Reviewers Reserve 1995 Ad hoc member, American Cancer Society, Scientific Advisory Committee on Personnel for Research 1995 Ad hoc member, Howard Hughes Medical Institute, Review Panel for Research Training for Medical Students 1996 Ad hoc member, NIH Experimental Virology Study Section 1996-1998 Member, Howard Hughes Medical Institute review panel Postdoctoral Research Fellowships for Physicians program 2000 Ad hoc member, Board of Scientific Counselors Review, NIH Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases 2003 Ad hoc member, NIH Experimental Virology Study Section 2004 Chair, NIH Center for Scientific Review Special Emphasis Panel 2005 Member, NIH Center for Scientific Review Special Emphasis Panel 2006 Ad hoc member, NIH Virology A Study Section 2007 Chair, NIH Center for Scientific Review: Topics in Virology Study Section 2008 Member, NIH Center for Scientific Review Special Emphasis Panel 2008 Ad hoc member, Board of Scientific Counselors Review, NIH Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases 2009 Ad hoc member, NIH Microbiology and Infectious Diseases (MID) Review Committee 2009 Ad hoc member, NIH Virology B Study Section 2010-2013 Member, NIH College of CSR Reviewers 2010 Ad hoc member, NIH Center for Scientific Review: Topics in Infectious Diseases and Microbiology Study Section 2011 Ad hoc reviewer, NIH Director's Pioneer Award Program 2012 Ad hoc member, NIH Center for Scientific Review: Topics in Virology Study Section 2013 Ad hoc reviewer, NIH Director's Pioneer Award Program 2013–2018 Member, NIH Virology B Study Section 2016-2018 Chair, NIH Virology B Study Section Awards and Academic Distinctions 1974-1979 National Institutes of Health Predoctoral Traineeship 1979-1980 American Cancer Society Postdoctoral Fellowship 1980-1983 National Institutes of Health National Research Service Award 1985 American Cancer Society Faculty Research Award Bert L. Semler Curriculum Vitae Page 4 Awards and Academic Distinctions (cont.) 1986-1991 National Institutes of Health Research Career Development Award 1997 Athalie Clark Outstanding Research Award; Research Associates College of Medicine, University of California, Irvine 2001 ISI Highly Cited Researcher 2007 Elected, Fellow of the American Academy of Microbiology 2008 The Bill Joklik Lecturer, 27th Annual Meeting of the American Society for Virology 2010 Senior Fellow of the American Asthma Foundation 2010 Elected, Fellow of the American Association for the Advancement of Science 2011 Elected, President of the American Society for Virology 2013 American Society for Microbiology Distinguished Lecturer Research Grants - Active Support: 1988-present National Institutes of Health: R01-AI026765, “Impact of host protein dynamics on picornavirus RNA 5’ noncoding region functions,” 4/22/16-3/31/20 (current funding period) 2014-present National Institutes of Health: R01-AI110782, “Role of host cell protein TDP2 as VPg unlinkase during picornavirus replication,” 2/1/14-1/31/20 (current funding period) 2016-present National Institutes of Health: R21-AI126879, “Defining the nuclear vs. cytoplasmic proteome during human rhinovirus infections,” 6/10/16-5/31/19 (current funding period) Training Grant - Active Support: 2002-present National Institutes of Health, “Molecular Biology of Eukaryotic Viruses “9/1/14-8/31/19 (current funding period) Invited Lectures and Session Chair at Scientific Meetings 1985 IVth Meeting of the European Study Group of the Molecular Biology of Picornaviruses; Seillac, France: Symposium speaker 1986 UCLA Symposium - Proteases in Biological Control and Biotechnology Park City, Utah: Symposium speaker 1988 ICN-UCI International Conference on Virology; Newport Beach, California Symposium speaker 1988 Picornavirus Symposium, Institute of Poliomyelitis and Viral Encephalitides of the USSR Academy of Medical Sciences; Moscow, U.S.S.R. Symposium speaker 1989 Banbury Center Conference on Viral Proteinases; Cold Spring Harbor, New York: Symposium speaker Bert L. Semler Curriculum Vitae Page 5 Invited Lectures and Session Chair at Scientific Meetings (cont.) 1989 Second International Symposium on Positive Strand RNA Viruses Vienna, Austria: Symposium speaker 1990 The 90th Annual Meeting of the American Society for Microbiology Anaheim, California: Invited speaker and session convenor 1990 The Fifth Rinshoken International Conference - Genetic Determinants of Picornavirus Diseases; Tokyo, Japan: Symposium speaker and session chairperson 1991 Keystone Symposium - Molecular Biology of Human Pathogenic Viruses Lake Tahoe, California: Symposium speaker 1991 Keystone Symposium - Proteolysis in Regulation and Disease; Keystone, Colorado: Symposium speaker 1992 NATO Advanced Study Institute and EEC Course: The Regulation of Gene Expression by Animal Viruses; Mallorca, Spain: Invited speaker and session chair 1994 VIIIth Meeting of the European Study Group on the Molecular Biology of Picornaviruses; Korpilampi, Finland: Symposium speaker 1995 First ICGEB-UCI Virology Symposium; New Delhi, India Invited speaker and session chair 1997 Keystone Symposium - RNA-Protein Interactions; Taos, New Mexico Symposium speaker 1998 Fifth International Symposium on Positive Strand RNA Viruses St. Petersburg, Florida: Symposium speaker 1998 Xth Meeting of the European Study Group on the Molecular Biology of Picornaviruses; Jena, Germany: Symposium speaker 1999 C.N.R.S. Jacques Monod Conference – New Insights into the Mechanism of mRNA Translation: The Significance of RNA Structure; Aussois, France Invited speaker 2001 Gordon Research Conference on Viruses and Cells; Tilton, New Hampshire Session chair 2001 Viruses, Cancer, and the Environment Symposium; Monterrey, Mexico Symposium speaker 2002 Picornavirus Research in 2002: Mini-symposium in honor of Dr. Ellie Ehrenfeld’s 30 years in the field; Bethesda, Maryland: Invited speaker 2002 XIIth Meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPICamerica 2002); Cape Cod, Massachusetts Symposium speaker 2004 Seventh International Symposium on Positive Strand
Load more

Recommended publications
  • Replication of Picornaviruses I
    JouRNAL oF VnoLoGy, Dec. 1975, p. 1512-1527 Vol. 16, No. 6 Copyright ©) 1975 American Society for Microbiology Printed in U.SA. Replication of Picornaviruses I. Evidence from In Vitro RNA Synthesis that Poly(A) of the Poliovirus Genome is Genetically Coded KAROLINE DORSCH-HASLER, YOSHIAKI YOGO,' AND ECKARD WIMMER* Department ofMicrobiology, St. Louis University School ofMedicine, St. Louis, Missouri 63104, and Department ofMicrobiology, School ofBasic Health Sciences, State University ofNew York at Stony Brook, Stony Brook, New York 11794 * Received for publication 3 July 1975 A crude replication complex has been isolated from poliovirus-infected HeLa cells and used for synthesis of poliovirus replicative intermediate (RI) RNA, replicative form (RF) RNA, and single-stranded (SS) RNA in vitro. All three classes of virus-specific RNA synthesized in vitro are shown to contain poly(A). Poly(A) of RF and of SS RNA [RF-poly(A) and SS-poly(A)] has a chain length (50 to 70 nucleotides) that is shorter than that of poly(A) of in vivo-synthesized RNAs. Poly(A) of RI [RI-poly(A)], however, is at least 200 nucleotides long and, therefore, larger than poly(A) of RI isolated from HeLa cells 4 h after infection. The crude membrane-bound replication complex contains a terminal adenylate transferase activity that is stimulated by Mn2+ and the addition of an (Ap),AoH primer. This transferase activity is found also in extracts of mock-infected cells. Partial purification of the replication complex in a stepwise sucrose gradient, in which the viral replicase is associated with the smooth cytoplasmic membrane fraction, does not remove the terminal transferase.
    [Show full text]
  • Abstract Book (Pdf)
    6th EMBO/EMBL Conference on Science and Society Science and Security 28 – 29 October 2005 at the European Molecular Biology Laboratory Heidelberg, Germany Organising Committee: Andrew Moore (EMBO, Chair), Halldòr Stefànsson (EMBL), Alessandra Bendiscioli (EMBO), Frank Gannon (EMBO), Iain Mattaj (EMBL) Welcome message n September this year, plans for a new bio-defence laboratory in Montana, USA, were Iunveiled. In the $66.5 million facility, researchers will apply similar scientific knowledge to that which makes bio-terrorism or biological warfare a possibility in the first place. The double-edged sword of science application can hardly be more evident. How successfully can we prevent the misuse of research findings that otherwise give us important insights into the workings of nature and prospects of useful applications? What would be the consequences of tackling this problem by constraining research, the mobility of researchers and the funding and publication of their research? Could we, indeed, ever successfully define “research that should not be done”? How much thought should scientists give to the downstream consequences of their work when deciding what to research, or which experiments to do? In June this year, the USA announced that it would delay the introduction of compulsory biometric passports for travellers from 27 countries in Europe and the Asia-Pacific region until October 2006. But whenever they appear, biometric passports will be part of normal life in the future. In the era of international terrorism, this could, indeed, lead to a safer world; an example of a beneficial use of science. On the other hand, it increases the perception by normal citizens that they are being unnecessarily “watched” by an all- seeing, all-controlling state – the so-called Big Brother of Orwell’s 1984.
    [Show full text]
  • “Let There Be Life”
    Focus: Brave New World “We have reason to believe that the disease is created by a man-made super pathogen...” AND MAN SAID, “LETa short story THERE BE LIFE” By Lawrence Valverde e have reason to believe the nearly 200 scientists simmered with “Wdisease is caused by a man- mumbled comments. The researcher made super-pathogen. The construc- whose speech had been interrupted tion seems to be loosely based on the straightened himself and raised his smallpox virus, but with significant voice in response. modifications, including genes encod- “I hear your concern, Dr. Boots, ing immune system antagonists. It but we have not confirmed whether or also seems to have been specifically not this is, in fact, a terrorist act…” engineered to resist known antivi- “Who else would do such a thing, ral…” Dr. Cervantes?” Dr. Boots demanded. “What do you mean specifically “It wasn’t necessarily done on engineered!” boomed an overweight purpose…” said Dr. Cervantes—the man as he hauled himself out of speaker—but he was interrupted by his chair. “Are you proposing that the clamor that erupted as scientists terrorists may have the technology jump up from their seats. Dr. Cervantes to synthetically create their own vi- frowned. Trying to speak over the ruses?” The surrounding panel of crowd was clearly a lost cause. When credit: Clipart courtesy FCIT. http://etc.usf.edu/clipart credit: Clipart courtesy FCIT. fall 2008 • Harvard Science Review 9 valverde.indd 9 2/9/2009 11:24:54 PM Focus: Brave New World did this whole business with artificially Dr. Boots sputtered.
    [Show full text]
  • Profile of Eckard Wimmer
    PROFILE Profile of Eckard Wimmer he finding caused an uproar. vitalism—the belief that chemicals in liv- Researchers at Stony Brook Uni- ing systems are somehow distinct from Tversity in New York had engi- the chemicals in inorganic systems, such neered poliovirus in a test tube as salt and rocks. That notion was shat- (1). The discovery, led by Eckard Wimmer, tered in 1828, when German chemist elected in 2012 to the National Academy Friedrich Wöhler synthesized the organic of Sciences, dispelled the belief that compound urea from inorganic pre- viruses require a live host to grow cursors (5). and spread. By the 1940s, theoretical physicists and The thought of synthetic viruses terrified biochemists had begun to wonder about an American public still reeling from 9/11 what constitutes life. Wimmer was en- and the subsequent anthrax attacks. What thralled. Biochemistry squared perfectly if the technology to engineer viruses with his worldview, the idea that something wound up in the hands of bioterrorists? as seemingly profound as life could be pared However, today, just a decade later, it is down to its simplest—chemical—form. widely accepted that the ability to engineer While completing his second post- viruses also allows researchers to develop doctoral fellowship at the University of viruses that work as synthetic vaccines, British Columbia in Vancouver in the to carry genetic material into a cell for use mid-1960s, Wimmer attended a talk on in gene therapies, or to preferentially viruses and had his eureka moment. “It attack cancer cells (2). was clear, even though it wasn’t men- Wimmer says his motivations for engi- tioned in that talk, that if viruses can be neering polio were strictly scientific.
    [Show full text]
  • NAT265 NEW Mx
    news Poliovirus advance sparks fears of data curbs Erika Check, Washington The recent laboratory synthesis of an infec- tious poliovirus is prompting fears among SCIENCE US biologists that the publication of results GETTY IMAGES deemed potentially useful to terrorists may be restricted. The virus was synthesized by a team led by virologist Eckard Wimmer at the State University of New York at Stony Brook. The results, published online by Science on 11 July (J. Cello, A. V. Paul & E. Wimmer Science 10.1126/science.1072266), sparked media reports worldwide suggesting that similar techniques might be used by terrorists to synthesize the Ebola virus or even smallpox. For Eckard Wimmer, his results brought a ‘scary Culture shock: researchers have synthesized this Geneticists fear that the report — the first realization’ that viruses can be recreated. infectious poliovirus in the laboratory. demonstration that a published genome can be turned into an infectious virus — will been used to demonstrate the technique. sequence information. He claims that the encourage calls for a clampdown on the open Wimmer defends his group’s decision to latest research proves that the world can publication of gene sequences of viruses. publish, saying that they are only pointing never stop vaccinating against polio. “You US government officials have been floating out the fact that terrorists could misuse cannot eliminate a virus from existence,” he the idea of such restrictions since the terror- published data. “We’ve been criticized for says. “Somebody can always resynthesize it, ist attacks last autumn (see Nature 414, playing into the hands of a bioterrorist, but and that’s a scary realization.” 237–238; 2001).
    [Show full text]
  • Defective Interfering Particles of Poliovirus I
    JOURNAL OF VIROLOGY, Apr. 1971, p. 478-485 Vol. 7, No. 4 Copyright © 1971 American Society for Microbiology Printed int U.S.A. Defective Interfering Particles of Poliovirus I. Isolation and Physical Properties CHARLES N. COLE, DONNA SMOLER, ECKARD WIMMER, AND DAVID BALTIMORE Departmenit of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 Received for publication 8 January 1971 A class of defective interfering (DI) poliovirus particles has been identified. The first was found as a contaminant of a viral stock; others have been isolated by serial passage at a high multiplicity of infection. The DI particles are less dense than standard virus and sediment more slowly. Their ribonucleic acid (RNA) sediments more slowly than standard RNA and has a higher electrophoretic mobility. Compe- tition hybridization experiments with double-stranded viral RNA indicate that DI RNA is 80 to 90'- of the length of standard RNA. The proteins of DI particles are indistinguishable from those of standard poliovirus. Defective interfering (DI) particles are found (ii) virus diluted serially in medium; (iii) HeLa cells in stocks of many different types of viruses (9). washed twice with medium and resuspended at 107/ml These particles are a natural form of mutant in medium plus 1 horse serum. A mixture of 0.2 which could be useful in the study of viral multi- ml of a given virus dilution with 0.6 ml of cells and 0.6 ml of agar were spread rapidly over base layer plication. They are also of interest because of plates at room temperature, and plates were incu- their possible role in viral disease processes (9).
    [Show full text]
  • Synthetic Biology: Synthesis and Modification of a Chemical Called Poliovirus
    Synthetic biology: Synthesis and modification of a chemical called poliovirus Steffen Mueller, Dimitris Papamichail, John Robert Coleman, Jeronimo Cello, Aniko Paul, Steven Skiena, and Eckard Wimmer SUNY–Stony Brook, U.S.A. Synthetic biology is a newly emerging scientific field, encompassing knowledge of different disciplines, such as engineering, physics, chemistry, computer sciences, mathematics, and biology. Synthetic biology aims to create novel biological systems with functions that do not exist in nature. There seem infinite possibilities of constructing unique derivatives of existing organisms (bacteria, yeast, viruses). Apart from designing novel building elements for engineering biological systems, a fundamental requirement in synthetic biology is the ability of large-scale DNA synthesis and DNA sequencing. Viruses can be described in chemical terms; the empirical formula of the organic matter of poliovirus being:1 C332,652H492,388N98,245O131,196P7,501S2,340. Placing these atoms into order, a particle of high symmetry emerges2 with all the properties required for its proliferation and “survival” in nature. These properties are encoded in the viral genome, which is a single stranded nucleic acid (RNA) of about 7,500 nucleotides. Guided by the published nucleotide sequence,3 we have recently synthesized the DNA equivalent of the polio RNA genome and converted it by simple biochemical manipulations in a cell-free environment (outside living cells) into authentic poliovirus particles.4 The synthesis of a replicating "organism" in the absence of a natural template was without precedence at the time of publication4 and it provoked widespread responses – good and bad. Poliovirus is a human virus that replicates after ingestion in the gastrointestinal tract.
    [Show full text]
  • Profile of Eckard Wimmer
    PROFILE Profile of Eckard Wimmer he finding caused an uproar. vitalism—the belief that chemicals in liv- Researchers at Stony Brook Uni- ing systems are somehow distinct from Tversity in New York had engi- the chemicals in inorganic systems, such neered poliovirus in a test tube as salt and rocks. That notion was shat- (1). The discovery, led by Eckard Wimmer, tered in 1828, when German chemist elected in 2012 to the National Academy Friedrich Wöhler synthesized the organic of Sciences, dispelled the belief that compound urea from inorganic pre- viruses require a live host to grow cursors (5). and spread. By the 1940s, theoretical physicists and The thought of synthetic viruses terrified biochemists had begun to wonder about an American public still reeling from 9/11 what constitutes life. Wimmer was en- and the subsequent anthrax attacks. What thralled. Biochemistry squared perfectly if the technology to engineer viruses with his worldview, the idea that something wound up in the hands of bioterrorists? as seemingly profound as life could be pared However, today, just a decade later, it is down to its simplest—chemical—form. widely accepted that the ability to engineer While completing his second post- viruses also allows researchers to develop doctoral fellowship at the University of viruses that work as synthetic vaccines, British Columbia in Vancouver in the to carry genetic material into a cell for use mid-1960s, Wimmer attended a talk on in gene therapies, or to preferentially viruses and had his eureka moment. “It attack cancer cells (2). was clear, even though it wasn’t men- Wimmer says his motivations for engi- tioned in that talk, that if viruses can be neering polio were strictly scientific.
    [Show full text]
  • Doctor Atomic and Doctor Genomic
    GENDER MEDICINE/VOL. 5, NO. 4, 2008 Editorial Doctor Atomic and Doctor Genomic As I watched Doctor Atomic, John Adams’ fascinating new production with The Metropolitan Opera that dramatizes the Manhattan Project’s creation of the atomic bomb, I applauded the brilliant portrayal of the dilemma that our greatest scientific achievements pose. Because of their completely novel and tremen- dous power, these achievements have the potential for both enormous good and unimaginably terrible consequences. The opera’s libretto used original sources, and in their own words described the gut-wrenching tensions among the scientists involved in making the bomb. No imagined dialogue could possibly have been more compelling. Edward Teller and Robert Wilson tried, unsuccessfully, to persuade their colleagues to petition President Truman to refrain from dropping the bomb on Japan. Yet Robert Oppenheimer, the complex, highly cultured, and charismatic project leader whom Adams dubbed “Dr. Atomic,” pushed the testing of the device to completion, despite his own misgivings. He later said, “As I watched the explosion, I thought of a quotation from the Bhagavad Gita: ‘I am become death, the destroyer of worlds.’” The average age of the more than 300 men and women who worked on the Manhattan Project was 25. At Los Alamos, beer drinking, lovemaking, and frenzied devotion to finishing the project were all, predict- ably, going on at once. Many worked to the point of exhaustion: one young scientist was hospitalized with a nervous breakdown. The opera touched on the thousand difficulties faced by Oppenheimer as he knit the project together despite the disparate enthusiasms, misgivings, and diametrically opposed moti- vations of the gifted scholars, many of them Nobel laureates, who worked to complete it.
    [Show full text]
  • Discover the World of Microbes Related Titles
    Gerhard Gottschalk Discover the World of Microbes Related Titles Dale, J. W., Park, S. F. Molecular Genetics of Bacteria 2010 ISBN: 978-0-470-74184-9 Krämer, R., Jung, K. (eds.) Bacterial Signaling 2010 ISBN: 978-3-527-32365-4 Feldmann, H. Yeast Molecular and Cell Biology 2010 ISBN: 978-3-527-32609-9 Wiegel, J., Maier, R., Adams, M. (eds.) Incredible Anaerobes From Physiology to Genomics to Fuels ISBN: 978-1-57331-705-4 Wilson, M. Bacteriology of Humans An Ecological Perspective 2008 ISBN: 978-1-4051-6165-7 Gerhard Gottschalk Discover the World of Microbes Bacteria, Archaea, and Viruses The Author Limit of Liability/Disclaimer of Warranty: While the publisher and author have used their best efforts in Prof. Dr. Gerhard Gottschalk preparing this book, they make no representations Institut für Mikrobiologie und or warranties with respect to the accuracy or Genetik completeness of the contents of this book and Georg-August-Universität specifically disclaim any implied warranties of Grisebachstr. 8 merchantability or fitness for a particular purpose. 37077 Göttingen No warranty can be created or extended by sales Germany representatives or written sales materials. The advice and strategies contained herein may not be Cover suitable for your situation. You should consult with The cover was designed by Anne a professional where appropriate. Neither the Kemmling, Göttingen. Source of publisher nor authors shall be liable for any loss of the micrographs (left to right: profit or any other commercial damages, including row 1, Anne Kemmling; row 2, but not limited to special, incidental, consequential, Manfred Rohde, Braunschweig, or other damages.
    [Show full text]
  • Celebratory Speech by Professor Dr
    Laudatio by Prof. Dr. Hans-Georg Kräusslich for Prof. Dr. Eckard Wimmer [Check against delivery.] [Address] “A Heart for Viruses” - that was the title of an article on Eckard Wimmer in the ‘My Career – Jobs and Opportunities’ column in the German newspaper Frankfurter Allgemeine Zeitung this July. It is surely no coincidence that Ernst-Ludwig Winnacker, the 2011 Robert Koch Gold Medal winner wanted to call one of his books “I Love Viruses”. Given that these pathogens cause many diseases, this title was deemed to have too much potential for misunderstanding, and changed to “Viruses, the Secret Rulers” in the end. This fascination with the astonishing properties of these miniscule pathogens and their importance for the evolution and development of illnesses is what unites Eckard Wimmer and Ernst-Ludwig Winnacker, and forms a link to last year’s award. By awarding him the Robert Koch Gold Medal, we are now honouring Eckard Wimmer's life's work and his great contribution to polio virus research, and to the development of molecular virology as a whole. Eckard Wimmer is a chemist, as evidenced by the way he approaches his research subjects and what drives him in his work. Of course, this is mainly the desire to understand an important pathogen and its ability to reproduce and spread, as well as to explain its pathogenic potential. He concentrated on the virus which causes infantile paralysis, the polio virus, an ancient pathogenic virus that wreaked havoc on mankind for many centuries. Today, the polio virus is one of the most thoroughly studied viruses, in no small part thanks to Eckard Wimmer’s research.
    [Show full text]
  • Effects of TDP2/Vpg Unlinkase Activity on Picornavirus Infections
    viruses Article Effects of TDP2/VPg Unlinkase Activity on Picornavirus Infections Downstream of Virus Translation 1, 2, 2 1, Autumn C. Holmes y, Guido Zagnoli-Vieira z , Keith W. Caldecott and Bert L. Semler * 1 Department of Microbiology & Molecular Genetics and Center for Virus Research, University of California, Irvine, CA 92697, USA; [email protected] 2 Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, UK; [email protected] (G.Z.-V.); [email protected] (K.W.C.) * Correspondence: [email protected] Present address: Department of Infectious Disease, Washington University School of Medicine, y St. Louis, MO 63110, USA. Present address: The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, z Cambridge CB2 1QN, UK. Received: 28 December 2019; Accepted: 22 January 2020; Published: 31 January 2020 Abstract: In this study, we characterized the role of host cell protein tyrosyl-DNA phosphodiesterase 2 (TDP2) activity, also known as VPg unlinkase, in picornavirus infections in a human cell model of infection. TDP2/VPg unlinkase is used by picornaviruses to remove the small polypeptide, VPg (Virus Protein genome-linked, the primer for viral RNA synthesis), from virus genomic RNA. We utilized a CRISPR/Cas-9-generated TDP2 knock out (KO) human retinal pigment epithelial-1 (hRPE-1) cell line, in addition to the wild type (WT) counterpart for our studies. We determined that in the absence of TDP2, virus growth kinetics for two enteroviruses (poliovirus and coxsackievirus B3) were delayed by about 2 h. Virus titers were reduced by ~2 log10 units for poliovirus and 0.5 log10 units for coxsackievirus at 4 hours post-infection (hpi), and by ~1 log10 unit at 6 hpi for poliovirus.
    [Show full text]