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PREPARED CULTURE MEDIA 091420ss PREPARED CULTURE MEDIA Made in the USA AnaeroGRO™ DuoPak A 02 Bovine Blood Agar, 5%, with Esculin 13 AnaeroGRO™ DuoPak B 02 Bovine Blood Agar, 5%, with Esculin/ AnaeroGRO™ BBE Agar 03 MacConkey Biplate 13 AnaeroGRO™ BBE/PEA 03 Bovine Selective Strep Agar 13 AnaeroGRO™ Brucella Agar 03 Brucella Agar with 5% Sheep Blood, Hemin, AnaeroGRO™ Campylobacter and Vitamin K 13 Selective Agar 03 Brucella Broth with 15% Glycerol 13 AnaeroGRO™ CCFA 03 Brucella with H and K/LKV Biplate 14 AnaeroGRO™ Egg Yolk Agar, Modified 03 Buffered Peptone Water 14 AnaeroGRO™ LKV Agar 03 Buffered Peptone Water with 1% AnaeroGRO™ PEA 03 Tween® 20 14 AnaeroGRO™ MultiPak A 04 Buffered NaCl Peptone EP, USP 14 AnaeroGRO™ MultiPak B 04 Butterfield’s Phosphate Buffer 14 AnaeroGRO™ Chopped Meat Broth 05 Campy Cefex Agar, Modified 14 AnaeroGRO™ Chopped Meat Campy CVA Agar 14 Carbohydrate Broth 05 Campy FDA Agar 14 AnaeroGRO™ Chopped Meat Campy, Blood Free, Karmali Agar 14 Glucose Broth 05 Cetrimide Select Agar, USP 14 AnaeroGRO™ Thioglycollate with Hemin and CET/MAC/VJ Triplate 14 Vitamin K (H and K), without Indicator 05 CGB Agar for Cryptococcus 14 Anaerobic PEA 08 Chocolate Agar 15 Baird-Parker Agar 08 Chocolate/Martin Lewis with Barney Miller Medium 08 Lincomycin Biplate 15 BBE Agar 08 CompactDry™ SL 16 BBE Agar/PEA Agar 08 CompactDry™ LS 16 BBE/LKV Biplate 09 CompactDry™ TC 17 BCSA 09 CompactDry™ EC 17 BCYE Agar 09 CompactDry™ YMR 17 BCYE Selective Agar with CAV 09 CompactDry™ ETB 17 BCYE Selective Agar with CCVC 09 CompactDry™ YM 17 BCYE with DGVP 09 CompactDry™ XSA 17 BCYE Selective Agar with GVPC 09 Derm-Duet™ II DTM/RSM Biplate 18 BE Agar 09 Derm-Duet™ RSM/DTM Biplate 18 BE Agar with Azide 09 Dermatophyte Milk Agar 18 BE Agar with Azide/CNA Biplate 09 Dermatophyte Test Medium Agar 18 BG Agar 10 SabDex Agar with C and G/DTM Biplate 18 BG Agar with Novobiocin 10 Dilu-Lok™ Pre-filled Dilution Vials 19 Headquarters BG Bile Broth with Durham Tube 10 Chocolate/MTM Biplate 20 1430 West McCoy Lane BHI Agar 10 Chocolate Agar with Bacitracin 20 Santa Maria, CA 93455 BHI Agar with Blood 10 CIN/MacConkey with Sorbitol Biplate 20 800.266.2222 : phone HardyDiagnostics.com BHI Agar with Blood and Gentamicin 11 CIN Agar 20 [email protected] BHI Agar, with Blood, Chloramphenicol, CLED Agar 20 and Gentamicin 11 CNA Agar 20 Distribution Centers BHI Agar with Chloramphenicol and CNA Agar/EMB Agar Biplate 20 Santa Maria, California Cycloheximide 11 CNA Agar/MacConkey Agar Biplate 21 Olympia, Washington Salt Lake City, Utah BHI Agar with Vancomycin 11 Columbia Agar, USP 21 Phoenix, Arizona BHI Broth 11 Columbia Agar, 5% Sheep Blood 21 Dallas, Texas Springboro, Ohio BHI Broth with 5% Fildes Solution 11 Columbia Agar with 5% Sheep Blood/ Lake City, Florida BiGGY Agar 11 MacConkey Agar Biplate 21 Albany, New York Raleigh, North Carolina Blood Agar, 5% 12 Cooked Meat with Iron, H and K 21 Blood Agar, 5%/Chocolate Biplate 12 Corn Meal Agar with Tween® 80 21 Blood Agar 5%/EMB Biplate 12 CT-SMAC 21 The Quality Management Blood Agar, 5%/MacConkey Biplate 12 CTA 22 System at the Hardy Diagnostics Blood Agar, 5% with Ampicillin 12 D/E Neutralizing Broth 22 manufacturing facility is certified to ISO 13485. Blood Agar, 8% 13 D/E Neutralizing Agar 22 Blood Agar, 10% 13 DNase Agar with Toluidine Blue 22 Blood Agar, EH 13 Diamonds Medium, Modified 22 Blood, Sheep, Defibrinated 13 Dichloran-Glycerol (DG-18) Agar 22 Bovine Blood Agar, 5% 13 EC Broth with Durham Tube 22 EC Broth with MUG iii22 Copyright © 2020 Hardy Diagnostics EE Broth Mossel, USP 23 Letheen Agar, Modified with MSA with Oxacillin 38 Egg Yolk Agar, Modified 23 Tween® 80, 1.5% 32 Mueller Hinton Agar 38 EMB Levine Agar 23 Letheen Agar/MacConkey Agar Biplate 33 Mueller Hinton Agar with Blood 39 EnteroScreen 4™ 23 Letheen Broth 33 Mueller Hinton Broth 39 EnviroTrans™ Swab Rinse Kit 24 Letheen Broth, Modified 33 Mycobiotic Agar 39 FB Broth 24 LIA 33 Mycoplasma Agar 39 Fluid A, USP 24 Liver Infusion Agar Selective 33 Mycoplasma Broth 39 Fluid D, USP 24 LJ Gruft 33 MycoVue™ Slide Culture System 40 Fluid K, USP 24 LJ Medium 33 MYP Agar 40 Fraser Broth, Modified 24 LJ with Ferric Ammonium Citrate, Slant 33 NaCl (Salt Tolerance) 6.5% Broth 40 FTM with Lecithin and Tween® 25 LJ with Pyruvate 33 Neomycin Anaerobic Blood Agar 40 FTM with Hemin and Vitamin K 25 LKV Agar 34 Neutralizing Buffer 40 GBS Detect™ 25 Loeffler’s Slant 34 Nitrate Broth with Durham Tube 41 GN Broth 25 m EI Agar 34 Non-Nutrient Agar Plate 41 Group A Beta Strep Agar 25 m Endo LES Agar 34 Nutrient Agar 41 Haemophilus ID Quadplate 25 m FC Agar with 1% Rosolic Acid 34 Nutrient Agar with MUG 41 HardyCHROM™ BluEcoli™ Biplate 26 m HPC Agar 35 Nutrient Broth 41 HardyCHROM™ Candida 26 m PA Agar 35 OF Media 41 HardyCHROM™ ECC 26 m TEC Agar, Modified 35 ONPG Rapid Test Broth 41 HardyCHROM™ ESBL 27 MacConkey Agar 35 Orange Serum Agar 41 HardyCHROM™ HUrBi™ 27 MacConkey Agar with Cefotaxime 35 Oxford Medium, Modified 41 HardyCHROM™ Listeria 27 MacConkey Agar with Sorbitol 35 Page’s Saline 41 HardyCHROM™ MRSA 27 MacConkey/EMB Biplate 35 PALCAM 41 HardyCHROM™ O157 28 MacConkey Broth 36 PC Agar 41 HardyCHROM™ Sakazakii 28 Malt Extract Agar 36 Phenylalanine Agar Slant 42 HardyCHROM™ Salmonella 28 Malt Extract Agar with Lecithin and Tween 36 PEA with 5% Sheep Blood 42 HardyCHROM™ Salmonella/XLT-4 Malt Extract Agar with 0.01% Peptic Digest Agar with Fildes Agar Biplate 28 Chloramphenicol 36 and Nafcillin 42 HardyCHROM™ SS NoPRO 28 Martin Lewis with Lincomycin 36 Peptone Water 42 HardyCHROM™ Staph aureus 29 Mastitis Triplate 37 Peptone Salt 42 HardyCHROM™ UTI 29 McFarland Standard 37 Phenol Red Broth with Durham Tube 42 HardyCHROM™ Vibrio 30 Middlebrook 7H10 Agar 37 Phosphate Buffered Saline (PBS), pH 6.8 42 HE Agar 30 Middlebrook 7H11/7H11 Selective Phosphate Buffered Saline (0.01M) with HE Agar/SS Agar Biplate 31 Agar Biplate 37 0.02% Tween® 80 42 HTM Agar 31 Middlebrook 7H11 Agar 37 Phosphate Buffered Saline, pH 7.5 43 Inhibitory Mold Agar 31 Middlebrook 7H11 Selective 37 Phosphate Buffer with Magnesium Inhibitory Mold Agar with Gentamicin 31 Middlebrook 7H11, Thin Pour 37 Chloride 43 Iodine-Iodide Solution 31 Middlebrook 7H9 Broth 37 Phosphate Buffered Saline with 0.05% KF Streptococcus Agar 31 Middlebrook 7H9 Broth with Tween® 80 37 Tween 20 43 KIA Slant 31 MIL Medium 37 PLET Agar 43 Lactobacilli MRS Agar 31 MIO Medium 38 Potato Dextrose Agar 43 Lactobacilli MRS Broth 32 Moeller’s Decarboxylase 38 Potato Dextrose Agar with Lactose Broth 32 Motility Test Medium with TTC 38 Chlortetracycline 43 Lauryl Tryptose Broth with Durham Tube 32 Motility Test Medium without TTC 38 Potato Dextrose Agar with Leeds Medium 32 MRSA Screen Plate 38 Chloramphenicol 43 Letheen Agar 32 MR-VP Broth 38 Potato Dextrose Agar with TA 43 Letheeniv Agar, Modified 32 MSA Agar 38 Potato Flake Agar 43 TCBS Agar 51 Urea Medium, Rapid 54 Tergitol 7 Agar with TTC 51 UVM Modified Listeria Broth 54 Tetrathionate Broth 51 V9 Agar 54 Tetrathionate Broth with Brilliant Green 51 Vogel and Johnson Agar 54 Thayer Martin Agar 51 VRBA 54 Thayer Martin Agar, Modified 52 VRBGA 54 Thioglycollate with H and K 52 Wallenstein Media 54 Thioglycollate without Indicator 52 Water with Tween® 80 54 Thioglycollate without Indicator, with XLD Agar 54 Supplements 52 XLT4 Agar 54 Thioglycollate with Indicator 52 XLT4 Agar/Brilliant Green Agar with Todd Hewitt Broth 52 Sulfadiazine Biplate 54 Todd Hewitt Broth with SXT 52 Tryptic Soy Broth (TSB), USP, Potato Flake Selective Agar 44 Trehalose Broth, Rapid 52 ReadyRack™ 56 PPLO Selective Agar 44 Tryptic Soy Agar 52 Fluid Thioglycollate Medium (FTM), USP, Pseudomonas Agar F 44 TSA, SterEM™, Irradiated, USP 52 ReadyRack™ 56 Pseudomonas Isolation Agar 44 TSA with Cycloheximide 52 R10 Broth 44 TSA with Lecithin and Tween® 80 52 Rapid Ornithine 44 TSA Blood/CLED/MAC 53 Regan-Lowe Agar 45 TSA with Lecithin and Tween® 80, USP 53 Regan-Lowe Semisolid 45 TSB, USP, Bottles 53 Rose Agar 45 TSB, Double Strength, USP 53 Rose/MacConkey Biplate 45 TSB Tubes 53 Rose Bengal Agar with Chloramphenicol 45 TSB with 6.5% Sodium Chloride 54 RTF Modified Casman Agar 46 TSB with Lecithin and Tween® 20, USP 54 RV Broth, USP 46 TSB with Lecithin and Tween® 80 54 SabDex Agar 46 TSI Agar 54 SabDex Agar, Emmons 46 Tryptone Broth, 2% 54 SabDex Agar with C and G/DTM Biplate 46 Tryptone Glucose Extract Agar 54 SabDex Agar with Chloramphenicol 46 Urea (Christensen’s Urea) Agar Slant 54 SabDex Agar with Chloramphenicol and Tetracycline 46 SabDex Agar with Gentamicin and Chloramphenicol 46 SabDex Agar with Lecithin and Tween® 80 46 SabDex Agar with Lecithin and Tween® 80, SterEM™, Irradiated 46 SabDex Broth 47 SabHI Agar 47 SabHI Agar with Blood, Chloramphenicol, and Cycloheximide 47 Saline, 0.45% 47 Saline, 0.85% 47 Saline, 0.9% with 0.5% Tween® 80 48 Selective Beta Strep Agar, Group A 48 Selective Strep Agar (COBA Medium) 48 Selenite Cystine Broth 48 SIM Medium 48 Simmons Citrate Agar 49 Skim Milk Agar 49 SP4 Agar 49 SP4 Broth 49 SS Agar 49 Standard Methods Agar 49 Sterile Deionized Water 49 Sterile Water 49 Strep B Carrot Broth™ One-Step 50 TAT Broth 51 TB Base Digestant 51 v SELECTION Since 1980, Hardy Diagnostics has grown to manufacture an extensive selection of prepared media products which are PREPARED packaged in plates, tubes, bottles, bags, and flasks.
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    PRODUCT CATALOG INDUSTRIAL MICROBIOLOGY BD Diagnostics Diagnostic Systems Table of Contents Table of Contents 1. Dehydrated Culture Media and Ingredients 5. Stains & Reagents 1.1 Dehydrated Culture Media and Ingredients .................................................................3 5.1 Gram Stains (Kits) ......................................................................................................75 1.1.1 Dehydrated Culture Media ......................................................................................... 3 5.2 Stains and Indicators ..................................................................................................75 5 1.1.2 Additives ...................................................................................................................31 5.3. Reagents and Enzymes ..............................................................................................75 1.2 Media and Ingredients ...............................................................................................34 1 6. Identification and Quality Control Products 1.2.1 Enrichments and Enzymes .........................................................................................34 6.1 BBL™ Crystal™ Identification Systems ..........................................................................79 1.2.2 Meat Peptones and Media ........................................................................................35 6.2 BBL™ Dryslide™ ..........................................................................................................80
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    Study of the vaginal and rectal microflora in pregnant women, with emphasis on Group B streptococci Nabil Abdullah El Aila Promoter: Prof. Dr. Mario Vaneechoutte Laboratory for Bacteriology Research. Department of Clinical Chemistry, Microbiology and Immunology Co-promoter: Prof. Dr. Marleen Temmerman Department of Obstetrics & Gynaecology Dissertation submitted in fulfilment of the requirements for the degree of Doctor in Biomedical Sciences Faculty of Medicine and Health Sciences, Ghent University January 2011 Nabil El Aila is supported by a PhD grant from (BOF, Bijzonder onderzoeksfonds) of Ghent University-Belgium. Dedication This thesis is dedicated to my lovely parents, Abdullah and Hanifa El Aila who taught me the value of education and have taken great pain to see me prosper in life. I am deeply indebted to them for their continued support and unwavering faith in me. It is also dedicated to my wife Aziza and my daughters: Danya, Dimah and Lana for their constant moral support, encouragement and invaluable help at every stage of my research. Members of the jury Prof. Dr. Phillip Hay Department of Genitourinary Medicine St George’s University of London, UK Prof. Dr. Pierrette Melin Belgian Reference Laboratory for Group B Streptococci Medical Microbiology Department, University Hospital of Liege Prof. Dr. Denis Pierard Department of Microbiology Vrij Universiteit Brussel Prof. Dr. Jean Plum Department of Clinical Chemistry, Microbiology and Immunology Ghent University Dr. Kristien Roelens Department of Obstetrics & Gynaecology Ghent University and Ghent University Hospital Prof. Dr. Koenraad Smets Department of Pediatrics Ghent University and Ghent University Hospital Table of the contents Members of the jury ........................................................................................................................................................... 1 Table of the contents ........................................................................................................................................................
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  • Comparison of Different Sampling Techniques and of Different Culture Methods for Detection of Group B Streptococcus Carriage in Pregnant Women

    Comparison of Different Sampling Techniques and of Different Culture Methods for Detection of Group B Streptococcus Carriage in Pregnant Women

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  • Bioprocess Engineering -1 – SBT1301

    Bioprocess Engineering -1 – SBT1301

    SCHOOL OF BIO AND CHEMICAL ENGINEERING DEPARTMENT OF BIOTECHNOLOGY UNIT – I– Bioprocess Engineering -1 – SBT1301 AN INTRODUCTION TO FERMENTATION PROCESSES The term “fermentation” is derived from the Latin verb fervere, to boil, thus describing the appearance of the action of yeast on the extracts of fruit or malted grain. The boiling appearance is due to the production of carbon dioxide bubbles caused by the anaerobic catabolism of the sugar present in the extract. However, fermentation has come to have with different meanings to biochemists and to industrial microbiologists. Its biochemical meaning relates to the generation of energy by the catabolism of organic compounds, whereas its meaning in industrial microbiology tends to be much broader. The catabolism of sugar is an oxidative process, which results in the production of reduced pyridine nucleotides, which must be reoxidized for the process to continue. Under aerobic conditions, reoxidation of reduced pyridine nucleotide occurs by electron transfer, via the cytochrome system, with oxygen acting as the terminal electron acceptor. However, under anaerobic condition, reduced pyridine nucleotide oxidation is coupled with the reduction of an organic compound, which is often a subsequent product of the catabolic pathway. In the case of the action of yeast on fruit or grain extracts, NADH is regenerated by the reduction of pyruvic acid to ethanol. Different microbial taxa are capable of reducing pyruvate to a wide range of end products, as illustrated in Fig. 1.1. Thus, the term fermentation has been used in a strict biochemical sense to mean an energy generation process in which organic compounds act as both electron donors and terminal electron acceptors.
  • Thesis Title

    Thesis Title

    Neonatal Invasive Infections Focused on Group B Streptococci Margrét Johansson Guðjónsdóttir Department of Pediatrics Institute of Clinical Sciences Sahlgrenska Academy, University of Gothenburg Gothenburg 2021 Cover illustration: Streptococcus agalactiae by Alissa Eckert - Medical Illustrator. A three-dimensional (3D), computer-generated image, of a group of Gram-positive, Streptococcus agalactiae (group B Streptococcus) bacteria. The artistic recreation was based upon scanning electron microscopic (SEM) imagery. Published with courtesy of CDC/ Antibiotic Resistance Coordination and Strategy Unit. Neonatal Invasive Infections – Focused on Group B Streptococci © Margrét Johansson Guðjónsdóttir 2021 [email protected] ISBN 978-91-8009-398-9 (PRINT) ISBN 978-91-8009-399-6 (ONLINE) http://hdl.handle.net/2077/68318 Printed in Borås, Sweden 2021 Printed by Stema Specialtryck AB “Happiness and bacteria have one thing in common; they multiply by dividing!” ― Rutvik Oza, mathematician Til fjölskyldunnar minnar (To my family) ABSTRACT Invasive infections affect neonates with the risk of severe morbidity and death, and Streptococcus agalactiae (Group B streptococcus, GBS) remains one of the most common pathogens. The aim of this thesis was to assess infections among neonates and infants, focusing on GBS to better understand prevention and treatment. Clinical data and outcomes were collected from patients’ medical records. Paper I was a prospective cohort study of GBS isolates obtained from adults and children with an invasive GBS infection in the years between 2004 and 2009. The study showed that among infants, serotype III was the most prevalent (48%), but serotype V (39%) was most common among adults. Paper II and III were observational, retrospective studies on early-onset (EO) and late-onset (LO) invasive infections among infants living within Gothenburg or five surrounding municipalities, from whom a pathogenic organism was isolated from blood or cerebrospinal fluid during the years 1997–2017.
  • Prevention of Perinatal Group B Streptococcal Disease Revised Guidelines from CDC, 2010

    Prevention of Perinatal Group B Streptococcal Disease Revised Guidelines from CDC, 2010

    Morbidity and Mortality Weekly Report www.cdc.gov/mmwr Recommendations and Reports November 19, 2010 / Vol. 59 / No. RR-10 Prevention of Perinatal Group B Streptococcal Disease Revised Guidelines from CDC, 2010 Continuing Education Examination available at http://www.cdc.gov/mmwr/cme/conted.html department of health and human services Centers for Disease Control and Prevention MMWR CONTENTS The MMWR series of publications is published by the Office of Surveillance, Epidemiology, and Laboratory Services, Centers for Introduction .............................................................................. 1 Disease Control and Prevention (CDC), U.S. Department of Health Methods ................................................................................... 2 and Human Services, Atlanta, GA 30333. Invasive Group B Streptococcal Disease ...................................... 3 Suggested Citation: Centers for Disease Control and Prevention. [Title]. MMWR 2010;59(No. RR-#):[inclusive page numbers]. Prevention of Early-Onset Group B Streptococcal Disease ............. 4 Identification of Candidates for Intrapartum Antibiotic Prophylaxis .. 6 Centers for Disease Control and Prevention Specimen Collection and Processing for GBS Screening ............... 7 Thomas R. Frieden, MD, MPH Director Secondary Prevention of Early-Onset GBS Among Infants .......... 10 Harold W. Jaffe, MD, MA Implementation and Impact of GBS Prevention Efforts ................. 11 Associate Director for Science Recommendations ..................................................................