DOCSLIB.ORG

Uy24-18874.Pdf

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Uy24-18874.Pdf UNIVERSIDAD DE LA REPÚBLICA - FACULTAD DE CIENCIAS PROGRAMANamaste DE POSGRADO EN BIOTECNOLOGÍA UTILIZACIÓN DE SECUENCIAS BARCODE PARA CLASIFICAR MUESTRAS DE ESPECIES VEGETALES DE INTERÉS INDUSTRIAL Tesis para optar por el título de Magister en Biotecnología Lic. Bioq. Fabiana Rey Bentos Montevideo, 2017 UNIVERSIDAD DE LA REPÚBLICA - FACULTAD DE CIENCIAS PROGRAMA DE POSGRADO EN BIOTECNOLOGÍA TESIS DE MAESTRÍA EN BIOTECNOLOGÍA "UTILIZACIÓN DE SECUENCIAS BARCODE PARA CLASIFICAR MUESTRAS DE ESPECIES VEGETALES DE INTERÉS INDUSTRIAL" Lic.Bioq. Fabiana Rey Bentos ORIENTADORES: TRIBUNAL: Dr. Fabián Capdevielle Dr. Claudio Martínez Dra. Mary Lopretti Dra. Alexandra Castro Dr. Fernando Alvarez Montevideo, 2017 2 AGRADECIMIENTOS Al Laboratorio Tecnológico del Uruguay (LATU) por permitirme llevar a cabo esta tesis y financiarla. A los miembros del Tribunal por acceder a corregirla, por sus opiniones, aportes y enseñanzas. A mis tutores por la paciencia y comprensión, y por no abandonar hasta el final. A mi familia por el apoyo y el sostén en los momentos mas difíciles, por el tiempo y los consejos. A mis amigos que tantas veces me oyeron hablar de "la tesis" y me dieron ánimo para seguir adelante. A mi jefe, Gustavo Domínguez, por su paciencia y palabras de aliento. A mis compañeros de trabajo por su apoyo y no dejarme flaquear hasta el final de este largo camino. A todos muchas gracias por estar de una forma u otra! 3 TABLA DE CONTENIDO AGRADECIMIENTOS ................................................................................................................ 3 TABLA DE CONTENIDO ............................................................................................................ 4 GLOSARIO Y ABREVIATURAS .................................................................................................... 6 LISTA DE FIGURAS Y TABLAS ..................................................................................................... 8 RESUMEN ........................................................................................................................... 11 1. INTRODUCCIÓN ................................................................................................................ 12 1.1. TRAZABILIDAD Y AUTENTICIDAD DE ALIMENTOS ............................................................. 12 1.2. PLANTAS AROMÁTICAS Y MEDICINALES ........................................................................ 14 1.2.1. Definiciones .................................................................................................... 14 1.2.2. Comercio internacional y antecedentes nacionales ........................................ 15 1.2.3. Características de las especies vegetales evaluadas. ....................................... 17 1.3. METODOLOGÍAS PARA EL RECONOCIMIENTO DE ESPECIES VEGETALES DE INTERÉS INDUSTRIAL ............................20 1.4. DNA BARCODING ..................................................................................................... 23 1.4.1. Generalidades ................................................................................................ 23 1.4.2. DNA barcoding en plantas .............................................................................. 26 1.4.3. Barcode of Life ................................................................................................ 29 1.4.4. Aplicaciones del DNA barcoding .................................................................... 31 1.5. PROCEDIMIENTOS PARA CLASIFICACIÓN DE MUESTRAS ................................................... 33 2. OBJETIVOS ...................................................................................................................... 35 2.1. OBJETIVO GENERAL ................................................................................................... 35 2.2. OBJETIVOS ESPECÍFICOS ............................................................................................. 35 3. METODOLOGÍA ................................................................................................................ 36 3.1. MATERIAL VEGETAL .................................................................................................. 36 3.2. EXTRACCIÓN DE ADN ............................................................................................... 37 3.3. OBTENCIÓN DE SECUENCIAS BARCODE .......................................................................... 39 3.3.1. Amplificación del locus rbcL ............................................................................ 39 3.3.2. Locus matK ..................................................................................................... 40 4 3.3.3. Secuenciación ................................................................................................. 41 3.4. ANÁLISIS DE DATOS MEDIANTE BARCODE OF LIFE DATA SYSTEMS (BOLD) ....................... 42 3.4.1. Ingreso de datos ............................................................................................. 42 3.4.2. Clasificación .................................................................................................... 43 3.4.3. Clasificación usando base de datos GenBank .................................................. 44 3.5. ANÁLISIS DE DATOS MEDIANTE UN PROCEDIMIENTO ESTADÍSTICO DE CLASIFICACIÓN (IMPLEMENTADO MEDIANTE PLATAFORMA WEKA)................................. .......................... 45 4. RESULTADOS Y DISCUSIÓN .................................................................................................. 46 4.1 EXTRACCIÓN DE ADN ................................................................................................ 46 4.2. OBTENCIÓN DE SECUENCIAS BARCODE .......................................................................... 50 4.2.1. Locus rbcL ...................................................................................................... 50 4.2.2. Locus matK ..................................................................................................... 57 4.3. ANÁLISIS DE DATOS MEDIANTE BARCODE OF LIFE DATA SYSTEMS (BOLD) .................. 60 4.3.1. Clasificación .................................................................................................... 60 4.3.2. Clasificación usando base de datos Genbank .................................................. 69 4.4. ANÁLISIS DE DATOS MEDIANTE UN PROCEDIMENTO ESTADÍSTICO DE CLASIFICACIÓN (IMPLEMENTADO MEDIANTE PLATAFORMA WEKA)........................... ................................ 76 5. CONCLUSIONES Y PERSPECTIVAS ........................................................................................... 90 6. REFERENCIAS BIBLIOGRÁFICAS ............................................................................................. 92 7. ANEXOS ........................................................................................................................ 101 5 GLOSARIO Y ABREVIATURAS ADN: ácido desoxirribonucleico ARFF: Attribute-Relation File Format Barcode: código de barras BID: Banco Interamericano de Desarrollo BOLD: Barcode of Life Data Systems BSA: seroalbúmina bovina CBI: Centre for the Promotion of Imports from Developing Countries CBOL: Consortium for the Barcode of Life CTAB: bromuro de cetiltrimetilamonio DBGW: Database Working Group DDBJ: DNA Database of Japan DMSO: dimetilsulfóxido DNA barcoding: metodología basada en el uso de una secuencia barcode para la identificación de la especie a la que pertenece un especimen dNTPs: desoxinucleótidos trifosfato EDTA: ácido etilendiaminotetraacético EMBL: European Molecular Biology Laboratory UE: Union Europea FAO: Organización de las Naciones Unidas para la Alimentación y la Agricultura FIDA: Fondo Internacional para el Desarrollo Agrícola FPTA: Fondo de Promoción de Tecnología Agropecuaria FUNDASOL: Fundación Uruguaya de Cooperación y Desarrollo Solidarios IAEA: Agencia Internacional de Energía Atómica 6 INIA: Instituto Nacional de Investigaciones Agropecuarias LATU: Laboratorio Tecnológico del Uruguay k-NN: algoritmo del vecino mas cercano (Nearest Neighbour algorithm) Locus: posición de un gen o secuencia en un cromosoma NCBI: National Center for Biotechnology Information MERCOSUR: Mercado Común del Sur OMS: Organización Mundial de la Salud PACPYMES: Programa de Apoyo a la Competitividad y Promoción de Exportaciones PCR: reacción en cadena de la polimerasa PVP: polivinilpirrolidona Secuencia barcode: secuencia de ADN proveniente de una región definida del genoma, utilizada para identificar especies TAE: solución tampón Tris - Acetato - EDTA USDA: United States Department of Agriculture USP: United States Pharmacopeia Voucher: muestra representativa de un organismo identificado taxonómicamente por un experto que se encuentra depositada en un herbario. 7 LISTA DE FIGURAS Y TABLAS Figura 1. Componentes principales del proyecto Barcode of Life (BOL) y su contribución a la taxonomía, reconstrucción de filogenias moleculares e investigaciones en genética de poblaciones. .................................................................................................................... 25 Figura 2. Poder de discriminación entre especies de los 7 loci por separado, y en combinaciones de 2 y 3. .................................................................................................. 28 Figura 3. Diagrama de las etapas y componentes del proceso de DNA barcoding ............. 30 Tabla 1. Descripción de los materiales vegetales de referencia utilizados. ....................... 36 Tabla 2.
Load more

Recommended publications
  • AGS Seed List No 61 2012-2013
    WELCOME TO THE ALPINE GARDEN SOCIETY’S 61st SEED LIST Please read through these notes and also the notes on the back of the order forms before completing the forms. The main distribution will begin in December and will continue into the New Year. Please note that the distribution takes place from Pershore and the office will be closed for the Christmas and New Year holiday. Consequently, no orders will be made up between 21 December and 7 January 2013 The seeds offered originate from various sources and cannot be guaranteed true to name. Neither The Alpine Garden Society nor any official of the Society can be held responsible for what is supplied. Members are reminded that named cultivars and hybrids cannot be relied upon to come true, and plants raised from seed from cultivars should not be labelled with the names of those cultivars. Seeds of many species are in short supply and we can never have enough to meet all requests. If you request very rare or scarce species you may be disappointed and we advise you to spread your requests throughout a variety of seeds on the list. We do limit the allocation of rare and sought after species to try and be as fair as possible to all members, especially those members who donate the rare seed. Surplus seeds are those remaining after all applications for main distribution seeds have been met. Please see the notes on the back of the order form for further information. On-line ordering is becoming the most popular way to order seed.
    [Show full text]
  • Empirically Derived Indices of Biotic Integrity for Forested Wetlands, Coastal Salt Marshes and Wadable Freshwater Streams in Massachusetts
    Empirically Derived Indices of Biotic Integrity for Forested Wetlands, Coastal Salt Marshes and Wadable Freshwater Streams in Massachusetts September 15, 2013 This report is the result of several years of field data collection, analyses and IBI development, and consideration of the opportunities for wetland program and policy development in relation to IBIs and CAPS Index of Ecological Integrity (IEI). Contributors include: University of Massachusetts Amherst Kevin McGarigal, Ethan Plunkett, Joanna Grand, Brad Compton, Theresa Portante, Kasey Rolih, and Scott Jackson Massachusetts Office of Coastal Zone Management Jan Smith, Marc Carullo, and Adrienne Pappal Massachusetts Department of Environmental Protection Lisa Rhodes, Lealdon Langley, and Michael Stroman Empirically Derived Indices of Biotic Integrity for Forested Wetlands, Coastal Salt Marshes and Wadable Freshwater Streams in Massachusetts Abstract The purpose of this study was to develop a fully empirically-based method for developing Indices of Biotic Integrity (IBIs) that does not rely on expert opinion or the arbitrary designation of reference sites and pilot its application in forested wetlands, coastal salt marshes and wadable freshwater streams in Massachusetts. The method we developed involves: 1) using a suite of regression models to estimate the abundance of each taxon across a gradient of stressor levels, 2) using statistical calibration based on the fitted regression models and maximum likelihood methods to predict the value of the stressor metric based on the abundance of the taxon at each site, 3) selecting taxa in a forward stepwise procedure that conditionally improves the concordance between the observed stressor value and the predicted value the most and a stopping rule for selecting taxa based on a conditional alpha derived from comparison to pseudotaxa data, and 4) comparing the coefficient of concordance for the final IBI to the expected distribution derived from randomly permuted data.
    [Show full text]
  • NJ Native Plants - USDA
    NJ Native Plants - USDA Scientific Name Common Name N/I Family Category National Wetland Indicator Status Thermopsis villosa Aaron's rod N Fabaceae Dicot Rubus depavitus Aberdeen dewberry N Rosaceae Dicot Artemisia absinthium absinthium I Asteraceae Dicot Aplectrum hyemale Adam and Eve N Orchidaceae Monocot FAC-, FACW Yucca filamentosa Adam's needle N Agavaceae Monocot Gentianella quinquefolia agueweed N Gentianaceae Dicot FAC, FACW- Rhamnus alnifolia alderleaf buckthorn N Rhamnaceae Dicot FACU, OBL Medicago sativa alfalfa I Fabaceae Dicot Ranunculus cymbalaria alkali buttercup N Ranunculaceae Dicot OBL Rubus allegheniensis Allegheny blackberry N Rosaceae Dicot UPL, FACW Hieracium paniculatum Allegheny hawkweed N Asteraceae Dicot Mimulus ringens Allegheny monkeyflower N Scrophulariaceae Dicot OBL Ranunculus allegheniensis Allegheny Mountain buttercup N Ranunculaceae Dicot FACU, FAC Prunus alleghaniensis Allegheny plum N Rosaceae Dicot UPL, NI Amelanchier laevis Allegheny serviceberry N Rosaceae Dicot Hylotelephium telephioides Allegheny stonecrop N Crassulaceae Dicot Adlumia fungosa allegheny vine N Fumariaceae Dicot Centaurea transalpina alpine knapweed N Asteraceae Dicot Potamogeton alpinus alpine pondweed N Potamogetonaceae Monocot OBL Viola labradorica alpine violet N Violaceae Dicot FAC Trifolium hybridum alsike clover I Fabaceae Dicot FACU-, FAC Cornus alternifolia alternateleaf dogwood N Cornaceae Dicot Strophostyles helvola amberique-bean N Fabaceae Dicot Puccinellia americana American alkaligrass N Poaceae Monocot Heuchera americana
    [Show full text]
  • VH Flora Complete Rev 18-19
    Flora of Vinalhaven Island, Maine Macrolichens, Liverworts, Mosses and Vascular Plants Javier Peñalosa Version 1.4 Spring 2019 1. General introduction ------------------------------------------------------------------------1.1 2. The Setting: Landscape, Geology, Soils and Climate ----------------------------------2.1 3. Vegetation of Vinalhaven Vegetation: classification or description? --------------------------------------------------3.1 The trees and shrubs --------------------------------------------------------------------------3.1 The Forest --------------------------------------------------------------------------------------3.3 Upland spruce-fir forest -----------------------------------------------------------------3.3 Deciduous woodlands -------------------------------------------------------------------3.6 Pitch pine woodland ---------------------------------------------------------------------3.6 The shore ---------------------------------------------------------------------------------------3.7 Rocky headlands and beaches ----------------------------------------------------------3.7 Salt marshes -------------------------------------------------------------------------------3.8 Shrub-dominated shoreline communities --------------------------------------------3.10 Freshwater wetlands -------------------------------------------------------------------------3.11 Streams -----------------------------------------------------------------------------------3.11 Ponds -------------------------------------------------------------------------------------3.11
    [Show full text]
  • Pollinator Plants for Stormwater Management Facilities
    Pollinator Plants for Stormwater Management Facilities PLSC 460 Adelaide Figurskey, Israel Orellana, Liz Pritchett March 8, 2021 Background Information Partnership for Action and Learning Sustainability (PALS) Project is in partnership with the Prince George’s County Department of Parks and Recreation Project Deliverables: Pollinator Plant Selection Guide ○ Brief literature review of stormwater management ○ Site considerations for three types of plantings (proper use of stormwater control measures and viewpoints)—a building, parking lot island, and open field ○ Plant palette fact sheet – plant needs and site environmental conditions with pictures Project Goals & Sample Sites Design a plant palette list for three potential stormwater management system sites: Site 1: Kentland Community Center, Landover, MD Site 2: View from a Building Site 3: Stormwater Pond Project Goals & Sample Sites Kentland Community Center Already established SWM systems Issues: heat in parking lot islands, lack of staff to control weeds, invasive plants, goose/deer presence Field trip Kentland Community Center Plant Palette Criteria Plant selection is based on these requirements: • Native: ~80%-100% native plant material • Growth Habit: aggressive establishment and spread to compete with invasives • Wildlife/Pollinators: Attractive to native wildlife and pollinators • Aesthetic Value: Aesthetically-pleasing and attractive to visitors • Low Maintenance • Deer Resistance • Soil Tolerance: Survivability in extreme dry/wet conditions Pollinator Plant Selection Guide Information
    [Show full text]
  • History of Botanical Collectors at Grandfather Mountain, NC
    HISTORY OF BOTANICAL COLLECTORS AT GRANDFATHER MOUNTAIN, NC DURING THE 19TH CENTURY AND AN ANALYSIS OF THE FLORA OF THE BOONE FORK HEADWATERS WITHIN GRANDFATHER MOUNTAIN STATE PARK, NC A Thesis by ETHAN LUKE HUGHES Submitted to the School of Graduate Studies at Appalachian State University in partial fulfillment of the requirements for the degree of Master of Science May 2020 Department of Biology HISTORY OF BOTANICAL COLLECTORS AT GRANDFATHER MOUNTAIN, NC DURING THE 19TH CENTURY AND AN ANALYSIS OF THE FLORA OF THE BOONE FORK HEADWATERS WITHIN GRANDFATHER MOUNTAIN STATE PARK, NC A Thesis by ETHAN LUKE HUGHES May 2020 APPROVED BY: Dr. Zack E. Murrell Chairperson, Thesis Committee Dr. Mike Madritch Member, Thesis Committee Dr. Paul Davison Member, Thesis Committee Dr. Zack E. Murrell Chairperson, Department of Biology Mike McKenzie, Ph.D. Dean, Cratis D. Williams School of Graduate Studies Copyright by Ethan L. Hughes 2020 All Rights Reserved Abstract History of botanical collectors at Grandfather Mountain, NC during the 19th century and an analysis of the flora of the Boone Fork headwaters Within Grandfather Mountain State Park, NC Ethan L. Hughes B.S. Clemson University Chairperson: Dr. Zack E. Murrell The Southern Appalachian Mountains have been an active region of botanical exploration for over 250 years. The high mountain peaks of western North Carolina, in particular, have attracted interest due to their resemblance of forest communities in NeW England and Canada and to their high species diversity. From the middle of the 19th century, Grandfather Mountain has been a destination for famous botanists conducting research in the region.
    [Show full text]
  • MAPEAMENTO DOS SÍTIOS DE Dnar 5S E 45S E ORGANIZAÇÃO DA CROMATINA EM REPRESENTANTES DA FAMÍLIA AMARYLLIDACEAE JAUME ST.-HIL
    EMMANUELLY CALINA XAVIER RODRIGUES DOS SANTOS MAPEAMENTO DOS SÍTIOS DE DNAr 5S E 45S E ORGANIZAÇÃO DA CROMATINA EM REPRESENTANTES DA FAMÍLIA AMARYLLIDACEAE JAUME ST.-HIL. RECIFE-PE 2015 i EMMANUELLY CALINA XAVIER RODRIGUES DOS SANTOS MAPEAMENTO DOS SÍTIOS DE DNAr 5S E 45S E ORGANIZAÇÃO DA CROMATINA EM REPRESENTANTES DA FAMÍLIA AMARYLLIDACEAE JAUME ST.-HIL. Tese apresentada ao Programa de Pós-Graduação em Botânica da Universidade Federal Rural de Pernambuco como parte dos requisitos para obtenção do título de Doutora em Botânica. Orientador: Prof. Dr. Reginaldo de Carvalho Dept° de Genética/Biologia, Área de Genética/UFRPE Co-orientador: Prof. Dr. Leonardo Pessoa Felix Dept° de Fitotecnia, UFPB RECIFE-PE 2015 ii MAPEAMENTO DOS SÍTIOS DE DNAr 5S E 45S E ORGANIZAÇÃO DA CROMATINA EM REPRESENTANTES DA FAMÍLIA AMARYLLIDACEAE JAUME ST.-HIL. Emmanuelly Calina Xavier Rodrigues dos Santos Tese defendida e _________________ pela banca examinadora em ___/___/___ Presidente da Banca/Orientador: ______________________________________________ Dr. Reginaldo de Carvalho (Universidade Federal Rural de Pernambuco – UFRPE) Comissão Examinadora: Membros titulares: ______________________________________________ Dra. Ana Emília de Barros e Silva (Universidade Federal da Paraíba – UFPB) ______________________________________________ Dra. Andrea Pedrosa Harand (Universidade Federal de Pernambuco – UFPE) ______________________________________________ Dr. Felipe Nollet Medeiros de Assis (Universidade Federal da Paraíba – UFPB) ______________________________________________ Dr. Marcelo Guerra (Universidade Federal de Pernambuco – UFPE) Suplentes: ______________________________________________ Dra. Lânia Isis Ferreira Alves (Universidade Federal da Paraíba – UFPB) ______________________________________________ Dra. Sônia Maria Pereira Barreto (Universidade Federal de Pernambuco – UFRPE) iii A minha família, em especial ao meu pai José Geraldo Rodrigues dos Santos que sempre foi o meu maior incentivador e a quem responsabilizo o meu amor pela docência.
    [Show full text]
  • INTRODUCTION This Check List of the Plants of New Jersey Has Been
    INTRODUCTION This Check List of the Plants of New Jersey has been compiled by updating and integrating the catalogs prepared by such authors as Nathaniel Lord Britton (1881 and 1889), Witmer Stone (1911), and Norman Taylor (1915) with such other sources as recently-published local lists, field trip reports of the Torrey Botanical Society and the Philadelphia Botanical Club, the New Jersey Natural Heritage Program’s list of threatened and endangered plants, personal observations in the field and the herbarium, and observations by other competent field botanists. The Check List includes 2,758 species, a botanical diversity that is rather unexpected in a small state like New Jersey. Of these, 1,944 are plants that are (or were) native to the state - still a large number, and one that reflects New Jersey's habitat diversity. The balance are plants that have been introduced from other countries or from other parts of North America. The list could be lengthened by hundreds of species by including non-persistent garden escapes and obscure waifs and ballast plants, many of which have not been seen in New Jersey since the nineteenth century, but it would be misleading to do so. The Check List should include all the plants that are truly native to New Jersey, plus all the introduced species that are naturalized here or for which there are relatively recent records, as well as many introduced plants of very limited occurrence. But no claims are made for the absolute perfection of the list. Plant nomenclature is constantly being revised. Single old species may be split into several new species, or multiple old species may be combined into one.
    [Show full text]
  • Photo Monitoring of High-Elevation Plants in Shenandoah National Park
    National Park Service U.S. Department of the Interior Natural Resource Stewardship and Science Photo Monitoring of High-Elevation Plants in Shenandoah National Park Natural Resource Report NPS/SHEN/NRR—2017/1542 ON THIS PAGE High-elevation Sibbaldiopsis tridentata site at Betty’s Rock Photograph by: Lea Korsmeyer, NPS ON THE COVER High-elevation Sibbaldiopsis tridentata site at Little Stony Man Photograph by: Lea Korsmeyer, NPS Photo Monitoring of High-Elevation Plants in Shenandoah National Park Natural Resource Report NPS/SHEN/NRR—2017/1542 Abigail R. Hyduke and Wendy B. Cass National Park Service Shenandoah National Park 3655 US Hwy 211 East Luray, VA November 2017 U.S. Department of the Interior National Park Service Natural Resource Stewardship and Science Fort Collins, Colorado The National Park Service, Natural Resource Stewardship and Science office in Fort Collins, Colorado, publishes a range of reports that address natural resource topics. These reports are of interest and applicability to a broad audience in the National Park Service and others in natural resource management, including scientists, conservation and environmental constituencies, and the public. The Natural Resource Report Series is used to disseminate comprehensive information and analysis about natural resources and related topics concerning lands managed by the National Park Service. The series supports the advancement of science, informed decision-making, and the achievement of the National Park Service mission. The series also provides a forum for presenting more lengthy results that may not be accepted by publications with page limitations. All manuscripts in the series receive the appropriate level of peer review to ensure that the information is scientifically credible, technically accurate, appropriately written for the intended audience, and designed and published in a professional manner.
    [Show full text]
  • The Use of Regional Phylogenies in Exploring the Structure of Plant Assemblages
    The use of regional phylogenies in exploring the structure of plant assemblages Tammy L. Elliott Doctor of Philosophy Department of Biology McGill University Montr´eal, Qu´ebec, Canada 2015-09-015 A thesis submitted to McGill University in partial fulfillment of the requirements of the degree of Doctor of Philosophy c Copyright Tammy L. Elliott, 2015 All rights reserved Dedication I dedicate this thesis to my parents, who sadly both left this world much to early. I like to dream that you are both enjoying your time together in a place with no worries, where you can enjoy all of the wonderful things in life. Dad—Although you left us when we were so young, I daily cherish the special times the two of us spent together. The memories of exploring the countryside, visiting neighbours, caring for the pigs and skipping school to fish are always close to my heart. Mom—I miss your strength, interesting perspective (albeit humorously pessimistic), no-nonsense attitude towards life and listening ear. I hope that you are finding ways to enjoy your grandchildren and tend your beautiful gardens. I would like to assure you that yes—one day I will have a full-time job. If Roses grow in Heaven Lord, please pick a bunch for me. Place them in my Mother’s arms and tell her they’re from me. Tell her that I love her and miss her, and when she turns to smile, place a kiss upon her cheek and hold her for awhile Because remembering her is easy, I do it every day, but there’s an ache within my heart that will never go away.
    [Show full text]
  • A List of Vascular Plant Species in the Nova Scotia Flora
    A list of vascular plant species in the Nova Scotia flora AFTER the page below was posted, The Atlantic Canada Conservation Data Centre posted their authoritative lists of Species Ranks for vascualar and non-vascular plants and for invertebrate and vertebrate animals for each of the Atlantic Provinces. See ACCDC Species Ranks. The page below remains posted for the time-being, but visitors are advised that the ACCDC Species Ranks pages are the better ones to consult. A list of native and naturalized vascular plant species in Nova Scotia was generated from Wild Species 2005: The General Status of Species in Canada using the General Status Search Tool. See Wild Species 2005:General Status Summaries:Vascular Plants for details about the listings. The Report parameters were: Report: "Ferns (2005), Orchids (2005), Vascular Plants (2005)" Status: "At risk, May be at risk, Sensitive, Secure, Undetermined, Not assessed, Exotic" Range: "NS" A total of 1657 records matched those criteria. The conservation rankings and number of species within each rank were as follows: Rank Description No. Species 1 At Risk 8 2 May Be At Risk 127 3 Sensitive 136 4 Secure 694 5 Undetermined 96 6 Not Assessed 0 7 Exotic 596 The list is convenient for looking up scientific names of species for which you have a common name or vice versa, also to see a list of all species within a family. Use your browser's FIND function to find a name within the page. Limitations Please note that only one common name for each species is given, also that synonyms for the scientific names are not listed.
    [Show full text]
  • Ontario Wetland Evaluation System
    OntariO Wetland evaluatiOn SyStem Southern manual 3rd edition, version 3.2 2013 © Queen’s Printer for Ontario 2013 OWES Revision History: 1st edition – 1981 2nd edition – March 1984 3rd edition – March 1993 3rd edition, 1st revision, May 1994 3rd edition, 2nd revision, December 2002 Cette publication spécialisé n’est pas disponible qu’en anglais MNR #62803 ISBN 978-1-4606-0200-3 Print ISBN 978-1-4606-0201-0 PDF ISSN 1192-8484 Harold Lee (Southern Science and Information Section) provided valuable contributions to the new substrate sections of the manual and to developing the Wetland Systems Key. Adam Hogg (Inventory Monitoring and Assessment Section) updated the sections on wetland mapping. Don Sutherland, Mike Oldham, Mike McMurtry, Tanya Taylor (all Natural Heritage Information Centre) and Bill Crins (Ontario Parks) reviewed sections and/or undertook analyses. Rich AcknOWlEdgEmEntS Russell created the new black duck maps, and Brigitte Collins reviewed the update to this section (both Environment Canada, Canadian Wildlife Service), (northern manual only). Other contributors and reviewers include: Gary Allen (MNR Midhurst), John Boos (MNR Southern Region), Peter Davis (MNR Northwest Region), Ron Huizer (Beacon Environmental), Peter Uhlig (MNR Ontario Forest Research Institute), Steve Varga (MNR Aurora), Ron Black (MNR Parry Sound), Anne Yagi (MNR Guelph), Dave Richards (MNR Aylmer), Debrupa Ontario Wetland Evaluation System, 3rd edition Pathak, Martha Allen, and Elizabeth Wright (all (version 3.2): Biodiversity Policy Section), Dave Webster (Ontario This 2013 version of the Ontario Wetland Evaluation Parks), and Ken Hare (MMAH). System (OWES) builds upon the work and input that a range of public, private and academic contributors made We gratefully acknowledge the support provided by to the previous editions of the OWES manuals.
    [Show full text]