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Sepiola Trirostrata Voss, 1962 Fig
Cephalopods of the World 169 Sepiola trirostrata Voss, 1962 Fig. 245 Sepiola trirostrata Voss, 1962a, Proceedings of the Biological Society of Washington, 75: 172 [type locality: Philippines]. Frequent Synonyms: None. Misidentifications: None. FAO Names: En – Knobby bobtail squid; Fr – Sépiole bosselée; Sp – Sepiola nudosa. tentacle II left hectocotylus III left I right I left IV left male arm arrangement (after Voss, 1963) dorsal view of male Fig. 245 Sepiola trirostrata Diagnostic Features: Fins short, do not exceed length of mantle anteriorly or posteriorly. Arms III in both sexes stout and strongly curved inward, more obviously so in males. Suckers in ventral series of right arm I and arms II of males larger than dorsal suckers. Hectocotylus present, left dorsal arm modified: proximal end with 2 slender fleshy papillae (anteriormost papilla longest) and dorsolateral to these a blunt tongue-like lobe, all formed from enlarged and elongate sucker pedicels; 2 rows of suckers on arm proximal to fleshy pad; distal end of hectocotylized arm with sucker pedicels enlarged and tightly packed to form 2 double rows of columnar structures; suckers reduced with tiny, fleshy, slit-like openings. Club with 4 large suckers in transverse rows; suckers differ in size; dorsal marginal longitudinal series of suckers larger than those in ventral marginal series. Paired kidney-shaped light organs present inside mantle cavity on each side of ink sac. Colour: Mantle and head with many minute brown or black chromatophores; arms III deep pink, arms I to III each with single longitudinal row of large chromatophores, arms IV with double row of small chromatophores. -
Ecological Diversification of Vibrio Fischeri Serially Passaged for 500 Generations in Novel Squid Host Euprymna Tasmanica
Microb Ecol DOI 10.1007/s00248-013-0356-3 HOST MICROBE INTERACTIONS Ecological Diversification of Vibrio fischeri Serially Passaged for 500 Generations in Novel Squid Host Euprymna tasmanica William Soto & Ferdinand M. Rivera & Michele K. Nishiguchi Received: 4 June 2013 /Accepted: 16 December 2013 # Springer Science+Business Media New York 2014 Abstract Vibrio fischeri isolated from Euprymna scolopes V. fischeri ecotypes, and complex changes in biolumines- (Cephalopoda: Sepiolidae) was used to create 24 lines that cence. Our data demonstrate that numerous alternate fitness were serially passaged through the non-native host Euprymna optima or peaks are available to V. fi sc he ri in host adaptive tasmanica for 500 generations. These derived lines were char- landscapes, where novel host squids serve as habitat islands. acterized for biofilm formation, swarming motility, carbon Thus, V. fischeri founder flushes occur during the initiation of source utilization, and in vitro bioluminescence. Phenotypic light organ colonization that ultimately trigger founder effect assays were compared between “ES” (E. scolopes)and“ET” diversification. (E. tasmanica) V. fischeri wild isolates to determine if conver- gent evolution was apparent between E. tasmanica evolved lines and ET V. fischeri. Ecological diversification was ob- Introduction served in utilization of most carbon sources examined. Con- vergent evolution was evident in motility, biofilm formation, The Sepiolid Squid–Vibrio Mutualism and select carbon sources displaying hyperpolymorphic usage in V. fischeri. Convergence in bioluminescence (a 2.5-fold Sepiolid squids in the genera Sepiola and Euprymna form light increase in brightness) was collectively evident in the derived organ mutualisms with marine bioluminescent bacteria from lines relative to the ancestor. -
Counterillumination in the Hawaiian Bobtail Squid, Euprymna Scolopes Berry (Mollusca: Cephalopoda)
Marine Biology (2004) 144: 1151–1155 DOI 10.1007/s00227-003-1285-3 RESEARCH ARTICLE B. W. Jones Æ M. K. Nishiguchi Counterillumination in the Hawaiian bobtail squid, Euprymna scolopes Berry (Mollusca: Cephalopoda) Received: 27 May 2003 / Accepted: 24 November 2003 / Published online: 10 January 2004 Ó Springer-Verlag 2004 Abstract The mutualism between the Hawaiian bobtail 1999), predator evasion (Hartline et al. 1999), and squid Euprymna scolopes and the luminescent symbiont counterillumination, an antipredatory behavior com- Vibrio fischeri has been used extensively as a model mon to many midwater cephalopods, decapod crusta- system for studies ranging from co-speciation and bio- ceans, and fishes (Young 1977; Harper and Case 1999; geography to gene regulation and the evolution of Lindsay et al. 1999). Animals exhibiting counterillumi- pathogenesis. In this association, the luminescent bac- nation reduce their silhouette by producing biolumi- terium V. fischeri is housed in a complex light organ nescence in an attempt to match the intensity and within the mantle cavity of E. scolopes. Prior hypotheses wavelength of down-welling light (Young and Roper have assumed that sepiolid squids in general utilize the 1977), providing a mechanism that allows them to evade bioluminescence produced by their V. fischeri symbionts predators by camouflage. The light produced can either for counterillumination, a behavior that helps squid be autogenic (luminescence produced intrinsically by the camouflage themselves by matching down-welling animal itself), or bacteriogenic (produced by bacterial moonlight via silhouette reduction. This assumption, symbionts). based solely on the morphology of the squid light organ, Establishing a morphological design for efficient has never been empirically tested for Euprymna in the counterillumination has resulted in the evolution of a laboratory. -
Cephalopoda: Sepiolidae)
Invertebrate Biology 137(3): 240–249. © 2018, The American Microscopical Society, Inc. DOI: 10.1111/ivb.12223 Vascular architecture in the bacteriogenic light organ of Euprymna tasmanica (Cephalopoda: Sepiolidae) Anthony J. Patelunas and Michele K. Nishiguchia Department of Biology, New Mexico State University, Las Cruces, New Mexico 88003-8001, USA Abstract. Symbiosis between southern dumpling squid, Euprymna tasmanica (Cephalopoda: Sepiolidae), and its luminescent symbiont, the bacterium Vibrio fischeri, provides an experi- mentally tractable system to examine interactions between the eukaryotic host and its bacte- rial partner. Luminescence emitted by the symbiotic bacteria provides light for the squid in a behavior termed “counter-illumination,” which allows the squid to mask its shadow amidst downwelling moonlight. Although this association is beneficial, light generated from the bacteria requires large quantities of oxygen to maintain this energy-consuming reaction. Therefore, we examined the vascular network within the light organ of juveniles of E. tas- manica with and without V. fischeri. Vessel type, diameter, and location of vessels were measured. Although differences between symbiotic and aposymbiotic squid demonstrated that the presence of V. fischeri does not significantly influence the extent of vascular branch- ing at early stages of symbiotic development, these finding do provide an atlas of blood ves- sel distribution in the organ. Thus, these results provide a framework to understand how beneficial bacteria influence the development of a eukaryotic closed vascular network and provide insight to the evolutionary developmental dynamics that form during mutualistic interactions. Additional key words: symbiosis, squid, vasculature, aerobic Symbiotic relationships between bacteria and mul- physiological changes during infection and colonization ticellular organisms are very common in nature by bacteria of the genus Vibrio from the environment (Hirsch & McFall-Ngai 2000; Baker 2003; Wang (Montgomery & McFall-Ngai 1998; Foster et al. -
Evolution of the Hectocotylus in Sepiolinae (Cephalopoda: Sepiolidae) and Description of Four New Genera
European Journal of Taxonomy 655: 1–53 ISSN 2118-9773 https://doi.org/10.5852/ejt.2020.655 www.europeanjournaloftaxonomy.eu 2020 · Bello G. This work is licensed under a Creative Commons Attribution License (CC BY 4.0). Monograph urn:lsid:zoobank.org:pub:0042EFAE-2E4F-444B-AFB9-E321D16116E8 Evolution of the hectocotylus in Sepiolinae (Cephalopoda: Sepiolidae) and description of four new genera Giambattista BELLO Arion, Via Colombo 34, 70042 Mola di Bari, Italy. [email protected] urn:lsid:zoobank.org:author:31A50D6F-5126-48D1-B630-FBEDA63944D9 …it is impossible to doubt that the [hectocotylized] arm is thereby adapted to some particular purpose, […] because its transformation occurs in so great a number of species of the class, and bears its peculiar characters in each natural genus. Japetus Steenstrup (1857) Abstract. The subfamily Sepiolinae (Mollusca: Cephalopoda: Sepiolidae), currently containing the genera Sepiola Leach, 1817, Euprymna Steenstrup, 1887, Inioteuthis Verrill, 1881, Rondeletiola Naef, 1921 and Sepietta Naef, 1912, is characterized by the hectocotylization of the left dorsal arm, i.e., its transformation into a copulatory organ thanks to modifications of sucker/pedicel elements. The hectocotylus morphology varies to a great extent across genera and species. In particular, one to several pedicels in its proximal third lose their sucker and become highly and diversely modified in shape to constitute a copulatory apparatus. An evolutionary gradient was observed in the copulatory apparatus morphology, from the simple modification into a papilla of just one pedicel from the third element of the ventral sucker row (some nominal species of Euprymna) to a quite complex structure involving several variously modified pedicels from both the ventral and dorsal sucker rows (Inioteuthis). -
Population Structure of Vibrio Fischeri Within the Light Organs Of
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Jan. 2009, p. 193–202 Vol. 75, No. 1 0099-2240/09/$08.00ϩ0 doi:10.1128/AEM.01792-08 Copyright © 2009, American Society for Microbiology. All Rights Reserved. Population Structure of Vibrio fischeri within the Light Organs of Euprymna scolopes Squid from Two Oahu (Hawaii) Populationsᰔ† M. S. Wollenberg and E. G. Ruby* University of Wisconsin—Madison, Department of Medical Microbiology and Immunology, 1550 Linden Drive, Madison, Wisconsin 53706-1521 Received 3 August 2008/Accepted 27 October 2008 We resolved the intraspecific diversity of Vibrio fischeri, the bioluminescent symbiont of the Hawaiian sepiolid squid Euprymna scolopes, at two previously unexplored morphological and geographical scales. These scales ranged from submillimeter regions within the host light organ to the several kilometers encompassing Downloaded from two host populations around Oahu. To facilitate this effort, we employed both novel and standard genetic and phenotypic assays of light-organ symbiont populations. A V. fischeri-specific fingerprinting method and five phenotypic assays were used to gauge the genetic richness of V. fischeri populations; these methods confirmed that the symbiont population present in each adult host’s light organ is polyclonal. Upon statistical analysis of these genetic and phenotypic population data, we concluded that the characteristics of symbiotic populations were more similar within individual host populations than between the two distinct Oahu populations of E. scolopes, providing evidence that local geographic symbiont population structure exists. Finally, to better understand the genesis of symbiont diversity within host light organs, the process of symbiosis initiation in newly hatched juvenile squid was examined both experimentally and by mathematical modeling. -
Euprymna Hyllebergi and Euprymna Tasmanica
Chapter 15 Euprymna hyllebergi and Euprymna tasmanica Jaruwat Nabhitabhata and Michelle K. Nishiguchi Abstract Bobtail squids of the genus Euprymna are small in size with a benthic habit. Such small size results in their insignificance in fisheries and aquaculture focused for human consumption. The unique ability of the voluntary adhesion system and symbiotic bacteria used for bioluminescence is now a primary research focus with potential industrial and biomedical applications. Their small size is well suited for the home aquarium with small volume. Culture of this cephalopod group can therefore serve both research and recreational purposes. Aquaculture in the laboratory provides valuable information for culture methodology that is uti- lized throughout the entire life cycle of several consecutive generations. This small size and benthic habit of Euprymna are advantageous for small-scale closed or open seawater culture systems. Major trends for culturing Euprymna are similar to other cephalopod groups, particularly benthic octopus that also produce planktonic hatchlings. Reduction of the cost of production is necessary for future large-scale production, with novel protocols for live feed requirements of planktonic young in the nursing phase. Keywords Euprymna · Small size · Benthic habit · Small-scale culture · Closed and open seawater systems · Research and recreational purposes 15.1 Importance of the Species The Thai bobtail squid, Euprymna hyllebergi Nateewathana 1997, is a common species occurring in the Andaman Sea of Thailand (Indian Ocean) and the Gulf of Thailand (Pacific Ocean; Nateewathana 1997; Nateewathana et al. 2001; Aungtonya et al. 2011). This species is small (20–40-mm mantle length, ML), J. Nabhitabhata () Excellence Centre for Biodiversity of Peninsular Thailand, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand e-mail: [email protected]; e-mail: [email protected] M. -
The Euprymna Scolopes -Vibrio Fischeri Symbiosis: a Biomedical Model for the Study of Bacterial Colonization of Animal Tissue
J. Molec. Microbiol. Biotechnol. (1999) 1(1): 13-21. The Squid-Vibrio SymbiosisJMMB as a Biomedical Symposium Model 13 The Euprymna scolopes -Vibrio fischeri Symbiosis: A Biomedical Model for the Study of Bacterial Colonization of Animal Tissue Edward G. Ruby* biological world. The principal interactions between bacteria and animal Pacific Biomedical Research Center tissue occur along the apical surfaces of host epithelial University of Hawaii, Manoa, Hawaii cells. Typically, a diverse consortium of microorganisms and host cells forms a dynamic, cooperating unit that helps maintain a healthy microenvironment within the colonized Abstract tissues. Such associations are ubiquitous, exhibit species specificity and, with few exceptions (Breznak and Brune, The diversity of microorganisms found in the marine 1994; Whittaker et al., 1996; Hooper et al., 1998), are poorly environment reflects the immense size, range of understood. A better understanding of the process by which physical conditions and energy sources, and bacteria and host cells exchange the signals that underlie evolutionary age of the sea. Because associations with these phenomena is an important research goal of living animal tissue are an important and ancient part biomedical research. Over the past decade the E. scolopes- of the ecology of many microorganisms, it is not V. fischeri association, a natural and experimentally facile surprising that the study of marine symbioses model system, has been used to discover the nature of (including both cooperative and pathogenic the mechanisms by which animals form beneficial interactions) has produced numerous discoveries of associations with microorganisms, and to determine how biotechnological and biomedical significance. The these mechanisms are related to, but have diverged from, association between the bioluminescent bacterium those that function in pathogenic infections. -
The Cephalopod Arm Crown: Appendage Formation and Differentiation in the Hawaiian Bobtail Squid Euprymna Scolopes
The cephalopod arm crown appendage formation and differentiation in the Hawaiian bobtail squid Euprymna scolopes Nödl, Marie-Therese; Kerbl, Alexandra; Walzl, Manfred G.; Müller, Gerd B.; de Couet, Heinz Gert Published in: Frontiers in Zoology DOI: 10.1186/s12983-016-0175-8 Publication date: 2016 Document version Publisher's PDF, also known as Version of record Document license: CC BY Citation for published version (APA): Nödl, M-T., Kerbl, A., Walzl, M. G., Müller, G. B., & de Couet, H. G. (2016). The cephalopod arm crown: appendage formation and differentiation in the Hawaiian bobtail squid Euprymna scolopes. Frontiers in Zoology, 13, [44]. https://doi.org/10.1186/s12983-016-0175-8 Download date: 08. Apr. 2020 Nödl et al. Frontiers in Zoology (2016) 13:44 DOI 10.1186/s12983-016-0175-8 RESEARCH Open Access The cephalopod arm crown: appendage formation and differentiation in the Hawaiian bobtail squid Euprymna scolopes Marie-Therese Nödl1,4* , Alexandra Kerbl2, Manfred G. Walzl3, Gerd B. Müller1 and Heinz Gert de Couet4 Abstract Background: Cephalopods are a highly derived class of molluscs that adapted their body plan to a more active and predatory lifestyle. One intriguing adaptation is the modification of the ventral foot to form a bilaterally symmetric arm crown, which constitutes a true morphological novelty in evolution. In addition, this structure shows many diversifications within the class of cephalopods and therefore offers an interesting opportunity to study the molecular underpinnings of the emergence of phenotypic novelties and their diversification. Here we use the sepiolid Euprymna scolopes as a model to study the formation and differentiation of the decabrachian arm crown, which consists of four pairs of sessile arms and one pair of retractile tentacles. -
University of Florida Thesis Or Dissertation Formatting
IMPACT OF MODELED MICROGRAVITY ON THE BENEFICIAL SYMBIOSIS BETWEEN THE HAWAIIAN BOBTAIL SQUID, EUPRYMNA SCOLOPES, AND ITS BIOLUMINESCENT PARTNER, VIBRIO FISCHERI By ALEXANDREA A. DUSCHER A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2019 © 2019 Alexandrea A. Duscher To my family and friends for their continuous love and support, to the incredible mentors that have helped guide me along the way, and to my partner for continuously encouraging me throughout it all. ACKNOWLEDGMENTS There are many individuals who have inspired and guided me along the way. Specifically, I would like to thank my mentor, Dr. Jamie Foster, for her support and advice throughout my journey. I would also like to thank Dr. Foster for providing me with my first research experience after my undergraduate studies and for guiding me towards pursuing a PhD. I would like to thank my committee members, Dr. Joseph Larkin III, Dr. Peter Kima, and my external committee member Dr. Anna-Lisa Paul. I would also like to thank the staff at the Department of Microbiology and Cell Science, specifically Jonathan Orsini for his willingness to assist me with whatever questions I had. I would like to thank the individuals at the Space Life Sciences Lab for their insight and friendship throughout my time working there. In addition, I would like to thank all of the previous and current lab members that have been a tremendous support through some of the best of toughest times, specifically I would like to thank Dr. -
Abstract Volume
ABSTRACT VOLUME August 11-16, 2019 1 2 Table of Contents Pages Acknowledgements……………………………………………………………………………………………...1 Abstracts Symposia and Contributed talks……………………….……………………………………………3-205 Poster Presentations…………………………………………………………………………………207-270 3 Venom Evolution of West African Cone Snails (Gastropoda: Conidae) Samuel Abalde*1, Manuel J. Tenorio2, Carlos M. L. Afonso3, and Rafael Zardoya1 1Museo Nacional de Ciencias Naturales (MNCN-CSIC), Departamento de Biodiversidad y Biologia Evolutiva 2Universidad de Cadiz, Departamento CMIM y Química Inorgánica – Instituto de Biomoléculas (INBIO) 3Universidade do Algarve, Centre of Marine Sciences (CCMAR) Cone snails form one of the most diverse families of marine animals, including more than 900 species classified into almost ninety different (sub)genera. Conids are well known for being active predators on worms, fishes, and even other snails. Cones are venomous gastropods, meaning that they use a sophisticated cocktail of hundreds of toxins, named conotoxins, to subdue their prey. Although this venom has been studied for decades, most of the effort has been focused on Indo-Pacific species. Thus far, Atlantic species have received little attention despite recent radiations have led to a hotspot of diversity in West Africa, with high levels of endemic species. In fact, the Atlantic Chelyconus ermineus is thought to represent an adaptation to piscivory independent from the Indo-Pacific species and is, therefore, key to understanding the basis of this diet specialization. We studied the transcriptomes of the venom gland of three individuals of C. ermineus. The venom repertoire of this species included more than 300 conotoxin precursors, which could be ascribed to 33 known and 22 new (unassigned) protein superfamilies, respectively. Most abundant superfamilies were T, W, O1, M, O2, and Z, accounting for 57% of all detected diversity. -
CEPHALOPOD SYMPOSIUM IV April 24–25, 2019 Proceedings Agenda
CEPHALOPOD SYMPOSIUM IV April 24–25, 2019 Proceedings Agenda DAY 1 Time Title Speaker 8:30–8:50am Welcome Moderator: Kathryn Kegel Informing the trophic dynamics of the giant Pacific octopus across 8:50–9:10am Ben Jevons Alaska through the application of stable isotope analysis Diet analysis of the burrowing octopus, Muusoctopus leioderma, using 9:10–9:30am Katherine Pekar stable isotopes Immune function in Octopus rubescens in response to ocean acidification 9:30–9:50am Monica Culler and warming Toward a sustainable, closed-system cephalopod breeding system: 9:50–10:10am laboratory culture of Japanese bobtail squids and the life cycle of Jeffery Jolly Euprymna berryi and Euprymna morsei An anecdotal guide to Euprymna scolopes husbandry at SEA LIFE 10:10–10:30am Brittany Stopp Kansas City 10:30–10:50am Break Moderator: Chris Van Damme The influence of nociceptive sensitization and self-referential visual cues 10:50–11:10am Ryan Howard from superficial injuries on camouflage behaviors of Indo-Pacific octopuses 11:10–11:30am Characterization of spectral discrimination strategies in the octopus Natalie Clay Visual response properties and functional organization of the 11:30–11:50am Judit Pungor octopus optic lobe 11:50am–12:10pm Collective decision-making in the arms of the octopus Dominic M. Sivitilli 12:10–12:30pm Octopuses in wild and domestic relationships David Scheel 12:30–2pm Lunch Moderator: Kaela Wuesthoff Trevor Erdmann, 2–2:20pm A brief retrospective on using the Octopus Behavioral Matrix Lance Hayes Implications of the physiological