Leaf Protein Extraction from Tropical Plants Lehel Telek Science and Education, Agricultural Research Service, Southern Region, U.S
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Leaf Protein Extraction From Tropical Plants Lehel Telek Science and Education, Agricultural Research Service, Southern Region, U.S. Department of Agriculture, Tropical Agriculture Research Station, Mayaguez, Puerto Rico hectare per year of leaf proteins can be at least four times higher than that of seed proteins. Protein and calorie malnutrition is widespread Leaf protein concentrates for animal feed cur- in the less developed countries. Importing high- rently are manufactured from alfalfa in Europe, and grade animal products, cereal grains, and animal a new processing plant was started recently in the feeds puts a strain on these countries’ economies. United States. Because efforts to adapt alfalfa to the Leaf protein concentrates (LPCs) should be serious- tropics have been only marginally successful in a ly considered as additional protein sources. Leaf few areas, possible tropical plant sources have been protein concentrates from alfalfa are prepared on investigated. a large commercial scale in Europe and the United In 1978, a broad research program to find suitable States. Unfortunately, alfalfa has not been grown tropical plants for leaf protein fractionation was or- successfully in the humid tropics, and a suitable ganized at the Tropical Agriculture Research Sta- tropical replacement is needed for leaf protein ex- tion, USDA (Science and Education) in Mayaguez, traction. Puerto Rico. At least 500 introductions were At least 500 introductions of tropical plants have planted and critically evaluated as potential sources been tested at the U.S. Department of Agriculture’s for LPC extraction (77) (table 1). The following cri- (USDA’s) Tropical Agriculture Research Station in teria were used in selecting plants suitable for LPC Puerto Rico. Potential plants for LPC production production: high protein and dry matter (DM) con- have been evaluated and selected for further tent, good protein extractability when freshly cut, research, Machinery has been developed which and good regrowth potential. The plants should fix may be suitable for laboratory, on-farm, village nitrogen, be erect and easily harvested mechanical- level, and commercial extraction. Further research ly, and be nontoxic and low in antinutritional is needed in agronomy and leaf protein extraction factors, and use. On-farm use is the most economical Freshly harvested plants were extracted in a system for the tropics, It is suggested that crude leaf blender with 600 ml ice water at high speed for 5 protein concentrate be used as human food only in to 10 minutes and filtered in a bag made of closely extreme emergencies. woven fabric. The pressed green juice was heated carefully in a 2-liter Erlenmeyer flask immersed in boiling water and agitated with a slow motion stir- Tropical Plants for Loaf rer. At 550 C, a green coagulum formed and was Protein Extraction separated by centrifugation, washed several times, and finally spread in thin layers on glass plates and At the present growth rate, world population will dried, The supernatant from the centrifugation was double in the next 30 to 40 years. This population heated carefully to 64° and the white curd increase mainly will burden the lesser developed coagulum that formed was separated by centrifuga- countries in the humid lowland tropics, where the tion, washed with acetone, and dried in a rotary current annual population growth rate is 2.3 per- evaporator. The liquid was further heated to 820. cent and yearly increase in food production re- After cooling, a light tan precipitate formed and mains low. The demand for food of plant origin will was processed as the 640 fraction (see fig. 1), continue to increase, and the supply of meat will The spontaneous coagulation of protein in the decrease because yields of cereal grains in the juice extracted from some plants was observed dur- tropics generally are low and local production is consumed by humans. This ever-widening food ing the survey of tropical plants. This phenomenon shortage cannot be alleviated by conventional agri- occurred during extraction of leaves of cassava, culture alone. As an additional source of protein, Leucaena leucocephala; some Indigoferas, leaf protein concentrates (LPCs) should be given Desmodium, and Mimosa species; and many of the serious attention because leaves are abundant year- tree legumes of Mimosa, Cassia, and Pea subfami- round in the tropics and many have high protein lies. These plants have been classified as Type I content. With suitable plant material, the yield per (table 1). Another group of plants yielded a green 78 Leaf Protein Extraction From Tropical Plants ● 79 Table 1 .—Crude Protein Content of Tropical Plants Amarantlius anclancaluius Hungary 12.3 26.6 Iv A. caudatus Sweden 13.0 27.7 Iv A. cruentus Taiwan 14.6 28.3 IV A. gangeticus Hungary 14.5 24.4 Ii’ A. hypochondriacus Sweden 11.5 27.9 Iv A. mantegazzianus Sweden 16.2 30.0 IV COMPOSITAE 11 Helian thus uniflorus Hungary 29.9 11 H . annuus Sweden 25.4 CRUCIFERAE Brassica alba Hungary 22.5 39.8 III B . campestris Pakistan 14.2 19.1 III Guatemala 10.8 21.0 III B. hirta Yugoslavia 10.4 30.0 III Poland 12.6 30.8 111 B. napus Hungary 11.8 21.1 111 France 11.6 26.4 III China 14.4 24.4 III Brassica juncea India 13.8 27.1 Cuba 12.2 22.3 Nepal 14.2 24.8 B. nigra Ind i a 10.2 26.4 Turkey 10.8 24.0 C r eec e 14.1 20.6 B. oleracea United States 14.0 19.4 B. “ var. gongyloides United States 12.6 19.4 Lepidium sativum Puerto Rico 12.3 17.6 Nasturtium officinale Puerto Rico 18.2 22.6 CUCURBITACEAE Benincasa hispida India 18.0 18.0 11 Lagenaria siceraria S. Africa 11.4 26.3 II Luffa cylindrical India 19.3 26.3 II EUPHORBIACEAE Cnidoscolus chayamansa Mexico 18.8 26.3 11 Manihot esculenta Colombia 20.8 25.5 I LECUMINOSAE Aeschynomene falcata Brazil 20.2 13.5 A. scabra Mexico 20.8 15.5 A. indica India 20.8 16.9 80 ● Plants: The Potentials for Extracting Protein, Medicines, and Other Useful Chemicals—Workshop Proceedings Alysicarpus vaginal is India 20.1 20.0 111 Ceylon 18.6 20.3 III Cajanus cajan India 24.0 22.5 111 Mexico 2 .0 20.6 111 Calopogonium muconoides Indonesia 20.0 19.7 111 Canavalia ensiformis India 21.5 18.4 111 Brazil 19.8 22.1 111 C. gladiata Philippines 20.9 21.9 111 Centrosema pubescens India 23.5 18.9 111 Philippines 25.0 23.2 111 Ivory Coast 20.0 19.4 111 Clitoria ternatea Brazil 16.1 23.6 111 Cuba 20.6 23.0 111 Australia 19.2 24.3 III Crotalaria alata India 18.6 19.9 II C. argyrolobioloides Kenya 20.4 23.9 II C. brachystachya Brazil 19.2 27.4 11 C. incana Argentina 19.3 22.9 II C. juncea India 21.4 25.8 11 USSR 24.0 26.3 II Cyamopsis tetragonoloba India 14.2 19.2 III Desmodium canum Brazil 20.8 18.9 I. D. distortum Hawaii 16.8 17.8 111 D. intortum Spain 25.0 18.7 III Brazil 25.0 23.5 111 D. perplexum Brazil 20.0 16.2 11 D. sandwicense Australia 21.7 23.0 1 Glycine wightii S. Africa 19.2 19.6 III Indigofera arrecta Chana 31.2 15.1 II I. brevipes Costa Rica 18.3 26.8 II -.Y cireinella Korea 17.6 26.2 I S. Africa 16.4 28.1 I. -.T colutea Australia 20.2 28.7 III I. confusa Indonesia 19.8 T 14.6 11 A. cryptantha S. Africa 19.8 24.0 111 I. echinata Tanzania 21.0 21.4 II X. hirsuta Nigeria 22.1 24.2 11 Brazil 21.0 27.9 II Rhodesia 23.4 30.6 II I. hochstetteri Rhodesia 18.3 21.2 II I. microcarpa Argentina 34.9 22.3 II I. mucronata Peru 20.8 22.4 II Brazil 23.7 24.4 11 I. recroflexa Kenya 21.8 21.2 11 I. schimperi Africa 14.6 23.3 11 I. semitijuga India 31.8 17.2 II I. spicata Tanzania 16.5 35.7 11 I. subulata Kenya 27.2 12.3 II Cuba 26.4 10.8 II I. suffruticosa Brazil 21.4 25.3 11 Mexico 20.7 32.4 11 I. sumatrana Australia 21.8 28.2 II I. tetlensis Africa 20.2 20.5 III I. Ceysmannii Malaya 21.6 31.0 11 I. tinctoria Dem. Republic 20.6 15.2 11 Leaf Protein Extraction From Tropical Plants ● 81 Lablab purpureus Malaysia 21.5 27.7 III Lupinus albus Turkey 18.8 19.5 111 L. angustifolius Hungary 20.4 18.8 111 S. Africa 22.6 16.6 III L. luteus Hungary 22.2 16.1 III Spain 20.4 19.2 III L. hispanicus Portugal 20.6 19.8 III Spain 20.2 19.2 111 Macroptilium lathyroides Australia 18.8 19.5 III Macrocyloma uniflorum Puerto Rico 18.6 26.4 III South Africa 21.0 21.3 III Mucuna deeringiana Mozambique 17.2 32.6 11 Phaseolus acontifolius India 18.8 18.3 111 Afghanistan 22.2 15.8 111 P. calcaratus Ivory Coast 19.7 20.8 111 Honduras 20.1 23.7 111 Psophocarpus tetragonolobus Puerto Rico 20.6 22.0 III Sesbania arabica Turkey 21.8 29.9 111 Afghanistan 22.2 30.0 111 S: cannabina India 19.8 21.4 111 S. exasperate’ Brazil 20.4 20.9 111 Argentina 20.6 20.6 III S.