QUICK DESIGN GUIDE Exposure of Fish in the Athabasca and Slave Rivers to Metals Potenally Derived from Oilsands Operaons QUICK TIPS (--THIS SECTION DOES NOT PRINT--) (--THIS SECTION DOES NOT PRINT--) 1 ,2 1 2 1,3,4 5 6 7 1,2 B. Tendler , E. Ohiozebau A. Hill , G. Codling , J.P. Giesy , E. Kelly , P. Hodson , J. Short , P.D. Jones This PowerPoint template requires basic PowerPoint (version 2007 or This PowerPoint 2007 template produces a 36”x72” professional poster. It 1.Toxicology Centre, University of Saskatchewan, Saskatoon, Canada 2.School of Environment & Sustainability, University of Saskatchewan, Saskatoon, Canada 3. Dept. of Biomedical Veterinary Sciences, University of Saskatchewan, Saskatoon, Canada newer) skills. Below is a list of commonly asked questions specific to will save you valuable time placing titles, subtitles, text, and graphics. 4. Dept. of Biology and Chemistry, City University of Hong Kong, Hong Kong, SAR, China 5. Environment and Natural Resources, Government of the Northwest Territories, Canada this template. 6. Department of Biology, Queens University, Kingston ON, Canada 7. DJWS Consulng LLC, Juneau AK, USA If you are using an older version of PowerPoint some template features Use it to create your presentation. Then send it to may not work properly. PosterPresentations.com for premium quality, same day affordable printing. Introducon Methods Results Results Using the template We provide a series of online tutorials that will guide you through the • Metal Site BB Mean ± SE (n) * p-value GE Mean ± SE (n)* p-value JF Mean ± SE (n)* p-value WE Mean ± SE (n)* p-value WF Mean ±SE (n)* p-value • Much of the current open-pit mining activities in the Alberta oilsand are Concentrations of Vanadium (V) were significantly (p<0.05) greater at Hg FMU 540±81 (3) 0.712 1251±252 (11) A 0.678 1416±256 (10) A 0.240 2475±733 (12) A <0.001 514±134 (10) A <0.001 poster design process and answer your poster production questions. adjacent to the Athabasca River. Fort Resolution and Fort Smith on the Slave River than those in fish from FM 691±162 (2) 1022±95 (20) A 1397±371 (19) A 1309±139 (20) A 179±21 (20) C FC 340±24 (4) 952±102 (19) A 1294±158 (20) A 811±78 (15) B 190±38 (20) C Verifying the quality of your graphics • Contaminants associated with oilsands include metals, polycyclic sites on the Athabasca River. FS 734±114 (8) A 973±125 (20) A 1427±311 (21) X 0.010 1813±264 (20) A 216±32 (18) B Go to the VIEW menu and click on ZOOM to set your preferred View our online tutorials at: FR 839±116 (30) A 1241±188 (12) A 1031±190 (21) A 1399±306 (10) A 353±44 (20) A aromatic hydrocarbons (PAHs), and naphthenic acids, some of which are • Concentrations of Selenium (Se) varied little among locations during Se FMU 1848±166 (3) 0.308 3060±340 (11) AB 0.005 1205±106 (13) B <0.001 1717±146 (13) BC <0.001 1612±133 (10) BC <0.001 magnification. This template is at 50% the size of the final poster. All http://bit.ly/Poster_creation_help FM 1476±121 (2) 2397±205 (20) B 843±118 (19) C 1432±137 (20) C 1546±78 (19) C persistent, toxic, mutagenic and potentially carcinogenic. summer. During the fall, selenium concentrations were greater in Slave FC 2282±164 (4) 3262±216 (19) AB 1639±105 (20) A 2041±106 (15) AB 1509±105 (20) C text and graphics will be printed at 200% their size. To see what your (copy and paste the link into your web browser). FS 1984±201 (8) A 3549±353 (20) A 1879±80 (21) A 2338±108 (20) A 2069±136 (18) AB • Concerns have been expressed about the potential effects of emissions River than Athabasca River. FR 1806±71 (30) A 4053±456 (12) A 1869±94 (21) A 2303±94 (10) AB 2233±89 (20) A poster will look like when printed, set the zoom to 200% and evaluate

on the environment and human health by communities in the Athabasca • Concentrations of Mercury (Hg) were greater in JF and WE on the Slave Tl FMU 8.94±0.42 (3) 0.003 11.51±1.50 (11) A 0.234 14.45±2.36 (13) B <0.001 20.94±3.61 (13) C <0.001 6.75±1.51 (10) B <0.001 the quality of all your graphics before you submit your poster for For assistance and to order your printed poster call FM 0.05 (2) 9.26±1.28 (20) A 8.02±1.32 (19) B 19.96±3.10 (20) C 6.32±0.70 (20) B River basin as well as by communities in the downstream Slave and River. FC 8.88±3.38 (4) 12.04±2.13 (19) A 16.84±2.22 (20) B 37.4±2.00 (15) B 6.85±1.26 (20) B printing. FS 8.43±1.36 (8) B 13.70±1.82 (20) A 32.82±4.00 (21) A 66.16±6.97 (20) A 20.30±2.70 (18) A PosterPresentations.com at 1.866.649.3004 Mackenzie river basins. Summer Fall FR 15.99±1.53 (30) A 15.44±2.98 (12) A 47.72±5.41 (21) A 78.54±7.96 (10) A 17.59±1.19 (20) A V FMU 46.6±2.0 (3) 0.031 22.3±5.4 (11) A 0.141 22.3±4.4 (13) A 0.208 25.3±5.8 (13) A 0.265 35.7±8.0 (10) A 0.096 Using the placeholders • Local communities have also reported an increase in the occurrence of FM 20.5±2.1 (2) 20.9±3.4 (20) A 16.3±2.8 (19) A 16.8±3.6 (20) A 18.8±3.4 (20) A FC 12.7±3.2 (4) 18.5±3.8 (19) A 23.7±5.1 (20) A 25.5±8.0 (15) A 37.0±5.6 (20) A To add text to this template click inside a placeholder and type in or lesions and deformities in fishes which has raised their concerns FS 66.3±16.4 (8) A 35.6±8.7 (20) A 25.0±5.7 (21) A 34.1±6.4 (20) A 40.7±8.4 (18) A FR 28.8±3.6 (30) B 36.0±4.2 (12) A 14.0±4.0 (21) A 17.7±5.7 (10) A 49.2±16.5 (20) A paste your text. To move a placeholder, click on it once (to select it), • These concerns have been heightened by uncertainties concerning the Object Placeholders Figure 3. Concentrations of metals by species and site. Summary statistics (means, SE = standard error of the place your cursor on its frame and your cursor will change to this origin of contaminants, natural or anthropogenic, and magnitudes of mean; n = sample size). Sites sharing a letter (A,B,C), within a metal/species grouping are not significantly symbol: Then, click once and drag it to its new location where exposure. different at α = 0.05 (Tu k e y family-wise error rate for multiple comparisons). Hypothesis tests conducted using ANOVA after data transformation (rank, log base 10, or power). Hg analyses conducted using ANCOVA with log you can resize it as needed. Additional placeholders can be found on Use the placeholders provided below to add new elements to your poster: base 10 transformed data (response and covariate) using length or weight as a covariate). X = significant the left side of this template. Objecves Quantification of Metals: interaction in ANCOVA (p = 0.05). Text in red addresses to subjectively assess trends across species. Drag a placeholder onto the poster area, size it, and click it to edit. This research was conducted to describe spatial and temporal • Approximately 0.1 g of dry muscle was added to a 15 mL Teflon vial and Conclusions Modifying the layout Section Header placeholder distributions of metals in tissues of fishes from selected locations along digested in concentrated nitric acid (69%) and hydrogen peroxide (30%). • Concentrations of Se were different in summer and fall. The increased This template has four Use section headers to separate topics or concepts within your the Athabasca and Slave Rivers. •Digests were evaporated until dry at approximately 70 ᴼC. concentration in summer could be caused by the influx of different column layouts. presentation. • 5 mL of 2% nitric acid was added to dried digest which was then filtered Right-click your mouse Methods contaminants after snow melt. (0.45 micrometre pore size) and transferred into 8 mL HDPE vial for • Thallium, but not V or Se appear to biomagnify, since fishes of higher on the background and Sample Collection: storage and analysis. click on “Layout” to see trophic levels have equal or higher concentrations than those at lower • Fish were collected in the summer fall and winter of 2011 and the spring • Concentrations of 20 metals were measured by ICP-MS. trophic levels. the layout options. of 2012 from 8 sites along the Athabasca Slave and Peace Rivers. The columns in the provided layouts are fixed and cannot be moved Text placeholder Results • V and Tl have been previously associated with discharges from • Target sample size for each site was 30 individuals of each of 5 species but advanced users can modify any layout by going to VIEW and then Move this preformatted text placeholder to the poster to add a new body petroleum based activities, including oil sands activities. (Goldeye - Hiodon alosoides, Whitefish - Coregonus clupeaformis, • Most of the metals analysed showed little or no location associated • Greater concentrations of metals along the Slave River are potentially SLIDE MASTER. of text. Northern Pike - Esox lucius, Walleye - Sander vitreus, Burbot - Lota lota). variability for the summer and fall samplings. due to upstream activities. • Thallium(Tl), Vanadium(V), Selenium(Se), and Mercury(Hg) did show Importing text and graphics from external sources • A total of 1498 fish were collected during these samplings. • Increases in V and Tl at the downstream sites pose interesting location associated variability. TEXT: Paste or type your text into a pre-existing placeholder or drag • Each fish was examined externally and internally. questions of bioaccumulation and chemodynamics, further research is • Se concentrations varied little among sites in summer, but in the fall planned. in a new placeholder from the left side of the template. Move it • Muscle, liver, bile, and blood plasma samples were taken for further were greater on the Slave River. • Spring sampling has been completed and metals are being quantified anywhere as needed. Picture placeholder analysis of indicators of condition and health. to better understand spatial and temporal distribution of their PHOTOS: Drag in a picture placeholder, size it first, click in it and Move this graphic placeholder onto your poster, size it first, and then click • A steady increase in the concentration of thallium in fish muscle was insert a photo from the menu. it to add a picture to the poster. noted at sites proceeding down the Athabasca/Slave system. concentrations. • Two sites were added downstream of Athabasca Basin for the spring TABLES: You can copy and paste a table from an external document • Thallium also showed a relationship between concentration and trophic sampling (FF, PP) to better understand metal distribution. onto this poster template. To make the text fit better in the cells of level as the higher trophic level JF and WE had greater concentrations. an imported table, right-click on the table, click FORMAT SHAPE then Acknowledgements click on TEXT BOX and change the INTERNAL MARGIN values to 0.25 BB GE JF WE WF decrease downriver This project would not have been possible without the assistance Goldeye (Hiodon alosoides) GE Pike or Jackfish (Esox lucius) JF Walleye or Pickeral (Sander vitreus) WE Hg increase at FS provided by First Nations and Métis communities of Ft Resolution, Ft Modifying the color scheme Se increase at FR Smith, Ft Chipewyan, Ft MacKay and Ft McMurray and by numerous To change the color scheme of this template go to the “Design” menu Provincial and Federal agencies. Funding was provided by the Boreal and click on “Colors”. You can choose from the provide color Tl increase downriver combinations or you can create your own. increase at FS, not high upstream Songbird Initiative (BSI), Aboriginal Affairs and Northern Development V Athabasca Canada (AANDC) and the Government of the Northwest Territories, which © 2012 PosterPresentaons.com Figure 1. Metal Concentration trends for metals of interest. Figure 2. Metal concentrations (V, Tl, Se) for all Species by Season. supported sampling in the NWT. Tim Barrett (UNB) provided assistance Student discounts are available on our Facebook page. 2117 Fourth Street , Unit C Go to PosterPresentations.com and click on the FB icon. Whitefish (Coregonus clupeaformis) WF Loche Mariah, Burbot (Lota lota) BB with statistical analysis. Berkeley CA 94710

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