Memories and Meanings from a Time of Turmoil
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Debating Diversity Following the Widely Publicized Deaths of Black Tape
KENNEDY SCHOOL, UNDER CONSTRUCTION. The Harvard Kennedy School aims to build students’ capacity for better public policy, wise democratic governance, international amity, and more. Now it is addressing its own capacity issues (as described at harvardmag.com/ hks-16). In January, as seen across Eliot Street from the northeast (opposite page), work was well under way to raise the level of the interior courtyard, install utility space in a new below-grade level, and erect a four-story “south building.” The project will bridge the Eliot Street opening between the Belfer (left) and Taubman (right) buildings with a new “gateway” structure that includes faculty offices and other spaces. The images on this page (above and upper right) show views diagonally across the courtyard from Taubman toward Littauer, and vice versa. Turning west, across the courtyard toward the Charles Hotel complex (right), affords a look at the current open space between buildings; the gap is to be filled with a new, connective academic building, including classrooms. Debating Diversity following the widely publicized deaths of black tape. The same day, College dean Toward a more inclusive Harvard African-American men and women at the Rakesh Khurana distributed to undergrad- hands of police. Particularly last semester, uates the results of an 18-month study on di- Amid widely publicized student protests a new wave of activism, and the University’s versity at the College. The day before, Presi- on campuses around the country in the last responses to it, have invited members of the dent Drew Faust had joined students at a year and a half, many of them animated by Harvard community on all sides of the is- rally in solidarity with racial-justice activ- concerns about racial and class inequities, sues to confront the challenges of inclusion. -
Efficient Genome Editing of an Extreme Thermophile, Thermus
www.nature.com/scientificreports OPEN Efcient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant Bjorn Thor Adalsteinsson1*, Thordis Kristjansdottir1,2, William Merre3, Alexandra Helleux4, Julia Dusaucy5, Mathilde Tourigny4, Olafur Fridjonsson1 & Gudmundur Oli Hreggvidsson1,2 Thermophilic organisms are extensively studied in industrial biotechnology, for exploration of the limits of life, and in other contexts. Their optimal growth at high temperatures presents a challenge for the development of genetic tools for their genome editing, since genetic markers and selection substrates are often thermolabile. We sought to develop a thermostable CRISPR-Cas9 based system for genome editing of thermophiles. We identifed CaldoCas9 and designed an associated guide RNA and showed that the pair have targetable nuclease activity in vitro at temperatures up to 65 °C. We performed a detailed characterization of the protospacer adjacent motif specifcity of CaldoCas9, which revealed a preference for 5′-NNNNGNMA. We constructed a plasmid vector for the delivery and use of the CaldoCas9 based genome editing system in the extreme thermophile Thermus thermophilus at 65 °C. Using the vector, we generated gene knock-out mutants of T. thermophilus, targeting genes on the bacterial chromosome and megaplasmid. Mutants were obtained at a frequency of about 90%. We demonstrated that the vector can be cured from mutants for a subsequent round of genome editing. CRISPR-Cas9 based genome editing has not been reported previously in the extreme thermophile T. thermophilus. These results may facilitate development of genome editing tools for other extreme thermophiles and to that end, the vector has been made available via the plasmid repository Addgene. -
32026062-MIT.Pdf
K.'-.- A, N E W Q UA D R A N G L E F O R C O R N E L L U N I V E R S I T Y A Thesis.submitted in partial fulfillment of the requirement s for the degree of Master of Architec ture at the Massachusetts Inst itute of Technology August 15, 1957 Dean Pie tro Bel lus ch Dean of the School of Archi tecture and P lanning Professor000..eO0 Lawrence*e. *90; * 9B. Anderson Head oythe Departmen ty6 Arc,hi tecture Earl Robert"'F a's burgh Bachelor of Architecture, Cornell University,9 June 1954 323 Westgate West Cambridge 39, Mass. August 14, 1957 Dean Pietro Belluschi School of Architecture and Planning Massachusetts Institute of Technology Cambridge 39, Massachusetts Dear De-an Belluschi, In partial fulfillment- of the requirements for the degree of Master of Architecture, I should like to submitimy thesis entitled, "A New Quad- rangle for Cornell University". Sincer y yours, -"!> / /Z /-7xIe~ Earl Robert Fla'nsburgh gr11 D E D I C A T I O N To my wife, Polly A C K N O W L E D G E M E N T S The development of this thesis has been aided by many members of the s taff at both M.I.T. &nd Cornell University. W ithou t their able guidance and generous assistance this t hesis would not have been possible. I would li ke to take this opportunity to acknowledge the help of the following: At M. I. T. -
2021「關懷有饍」中小企食肆資助計劃 "Care and Share" SME Caterers Subsidy Scheme 2021 參與食肆名單 List of Participating Eateries
2021「關懷有饍」中小企食肆資助計劃 "Care and Share" SME Caterers Subsidy Scheme 2021 參與食肆名單 List of Participating Eateries 食肆名稱 NAME OF EATERY 食肆地址 ADDRESS OF EATERY 東區 EASTERN DISTRICT 美食一族 Delicious Snack Food 小西灣小西灣道18號富景花園商場地下42號鋪 Shop 42, G/F, Fullview Garden Shopping Centre, 18 Siu Sai Wan Road, Siu Sai Wan 滋味棧甜品快餐店 Chi Mei Chen Desert & Fast Food Shop 小西灣小西灣道23號富怡花園地下45號鋪 Shop 45, G/F., Cheerful Garden, 23 Siu Sai Wan Road, Siu Sai Wan 理想店旺旺咖啡座 Perfect Cafe 小西灣小西灣道28號藍灣半島廣場地下6號鋪 Shop 6, G/F, Island Resort Mall, 28 Siu Sai Wan Road, Siu Sai Wan Shop 303, 3/F, Siu Sai Wan Plaza, Siu Sai Wan Estate, 10 Siu Sai Wan Road, Siu Sai 龍悅 Dragon Delight 小西灣小西灣道10號小西灣邨小西灣廣場3樓303號鋪 Wan 阿詩瑪雲南風味軒 Ashima Yunnan Restaurant 太古城太古城道18號太古城中心地下003-004號鋪 Shop 003-004, G/F, Cityplaza, 18 Taikoo Shing Road, Taikoo Shing 太古城太古城道26號第4期齊宮閣地下G411號鋪及 G/F, T-24 Han Kung Mansion & Shop G411, Chai Kung Mansion, Stage IV, 26 Taikoo 華昌粥麵 Wah Cheong Congee & Noodles 漢宮閣(24座)地下 Shing Road, Taikoo Shing 樂意粉麵 Lok Yee Restaurant 北角七姊妹道193C至195號地下 G/F, 193C-195 Tsat Tsz Mui Road, North Point 東泰茶餐廳 Tung Tai Restaurant 北角七姊妹道106至110號富邦大廈地下2號鋪 Unit 2, G/F, Fu Bong Mansion, 106-110 Tsat Tsz Mui Road, North Point 海洋餐廳 Ocean Restaurant 北角丹拿道52-58號地下D號鋪 Shop D, G/F., 52-58 Tanner Road, North Point 姨婆瑪麗 Ipoh Mari 北角北角道10號北角亞太商業中心地下1號鋪 Shop 1, G/F, NPAC, 10 North Point Road, North Point 小城鎮 Little Town 北角明園西街2-6號明苑中心地下4號鋪 Shop No. 4, G/F., Ming Yuen Centre, 2-6 Ming Yuen Western Street, North Point 知粥嘗樂 Congee Wonderland 北角英皇道175號地下 G/F, 175 King's Road, North Point 海家.小品 Aquadeli 北角英皇道431號安寧大廈地下4號鋪 Shop No. -
Consummate Coach Tim Murphy’S Formidable Game S:7”
Daniel Aaron • Max Beckmann’s Modernity • Sexual Assault November-December 2015 • $4.95 Consummate Coach Tim Murphy’s formidable game S:7” Invest In What Lasts How do you pass down what you’ve spent your life building up? A Morgan Stanley Financial Advisor can help you create a legacy plan based on the values you live by. So future generations can benefit from not just your money, but also your example. Let’s have that conversation. morganstanley.com/legacy S:9.25” © 2015 Morgan Stanley Smith Barney LLC. Member SIPC. CRC 1134840 04/15 151112_MorganStanley_Ivy.indd 1 9/21/15 1:59 PM NOVEMBER-DECEMBER 2015 VOLUME 118, NUMBER 2 FEATURES 35 Murphy Time | by Dick Friedman The recruiter, tactician, and educator who has become one of the best coaches in football 44 Making Modernity | by Joseph Koerner On the meanings and history of Max Beckmann’s iconic self-portrait p. 33 48 Vita: Joseph T. Walker | by Thomas W. Walker Brief life of a scientific sleuth: 1908-1952 50 Chronicler of Two Americas | by Christoph Irmscher An appreciation of Daniel Aaron, with excerpts from his new Commonplace Book JOHN HARVard’s JournAL 41.37. 41.37. R 17 Smith Campus Center under wraps, disturbing sexual-assault ULL IMAGE F findings, a law professor plumbs social problems, the campaign OR F NIVERSITY crosses $6 billion, cutting class for Christmas, lesser gains U and new directions for the endowment, fall themes and a SSOCIATION FUND, B A ARVARD H brain-drain of economists, Allston science complex, the Under- USEUM, RARY, RARY, B M graduate on newfangled reading, early-season football, and I L a three-point shooter recovers her stroke after surgery DETAIL, PLEASE 44 SEE PAGE EISINGER R OUGHTON H p. -
Extracurriculars Ney to a Site Held Sacred by the Hopi Peo- Ple, and O≠Ers Insight Into the Lives of the Archaeologists Themselves
New EnglandREGIONAL SECTION cial and historic significance of this jour- Extracurriculars ney to a site held sacred by the Hopi peo- ple, and o≠ers insight into the lives of the archaeologists themselves. SEASONAL Saunter down to the river with a picnic to • Continuing: Fragile Memories: Images OF THE PEABODY The Farmers’ Market at Harvard watch athletes from around New Eng- of Archaeology and Community at Copán, www.dining.harvard.edu/flp/ag_mar- land—and the world—compete in the 1891-1900. The exhibit explores one of ket.html. Open through October. annual two-day event. the most important Maya sites and its in- In Cambridge: fluence on the local community. • Tuesdays, 12:30-6 p.m. EXHIBITIONS Harvard Museum of Natural Outside the Science Center, at the corner The Harvard Art Museum History of Oxford and Kirkland streets. Please note: The Fogg and Busch-Rei- www.hmnh.harvard.edu In Allston: singer Museums are closed to the public 617-495-3045 • Wednesdays, 3-7 p.m. for renovations that are expected to last • October 16 at 6 p.m. Corner of North Harvard Street and about five years. Lecture and book signing with Eric Chi- Western Avenue. www.artmuseums.harvard.edu vian, director of Harvard’s Center for The markets, organized by Harvard Uni- 617-495-9400/9422 Health and the Global Environment, and versity Dining Services, o≠er fresh pro- • Opening September 13 coeditor of the new Sustaining Life: How duce, herbs, baked goods, and other de- Re-View, at the Sackler Museum, fea- Human Health Depends on Biodiversity. -
Comparative Genomics of the Restriction-Modification Systems in Helicobacter Pylori
Comparative genomics of the restriction-modification systems in Helicobacter pylori Lee-Fong Lin, Janos Posfai, Richard J. Roberts, and Huimin Kong* New England Biolabs, Inc., 32 Tozer Road, Beverly, MA 01915 Communicated by Charles R. Cantor, Sequenom Industrial Genomics, Inc., San Diego, CA, December 22, 2000 (received for review October 31, 2000) Helicobacter pylori is a Gram-negative bacterial pathogen with a small are subject to loss or gain of a repeat unit, presumably through genome of 1.64–1.67 Mb. More than 20 putative DNA restriction- slipped-strand mispairing during replication. This results in frame- modification (R-M) systems, comprising more than 4% of the total shifting, which can alternatively activate or inactivate genes (10). genome, have been identified in the two completely sequenced H. Tetranucleotide repeats were found in a Type III DNA methylase pylori strains, 26695 and J99, based on sequence similarities. In this gene and the length of the repeat tract determined the phase study, we have investigated the biochemical activities of 14 Type II variation rate (11). In the case of the H. pylori 26695 genome, 27 R-M systems in H. pylori 26695. Less than 30% of the Type II R-M putative genes that contain simple sequence repeats and that may systems in 26695 are fully functional, similar to the results obtained be subject to phase variation have been identified. These putative from strain J99. Although nearly 90% of the R-M genes are shared by phase-variable elements can be divided into three groups: lipopoly- the two H. pylori strains, different sets of these R-M genes are saccharide (LPS) biosynthesis, cell-surface-associated proteins and functionally active in each strain. -
1969 Compassion and Care
Justice Holmes • Inflammation • Harry Widener MAY-JUNE 2019 • $4.95 Compassion 1969 and Care Physician-Poet Rafael Campo Reprinted from Harvard Magazine. For more information, contact Harvard Magazine, Inc. at 617-495-5746 May 2019 Dear Reader, In 1898, an association of Harvard graduates established the Harvard Alumni Bulletin, “to give selected and summarized Harvard news to graduates who want it” and “to serve as a medium for publishing promptly all notices and announcements of interest to graduates.” members and students extend the limits of discovery and human understanding—in service to an ever more far- ung, diverse group of alumni around the globe. Today, nearly a century and a quarter later, the name has changed, to Harvard Magazine (as have the look and contents), but the founding Your Harvard Magazine can capture alumni voices (see the letters responding to the March-April principles have not: feature on the events of April 1969, beginning on page 4 of this issue), dive deep into critical research (read the feature on the scientists exploring in ammation, and how their work contributes • e magazine exists to serve the interests of its readers (now including all University to understanding disease, on page 46), and keep you current on the critical issues facing higher alumni, faculty, and sta )—not any other agenda. education on campus and around the world (see John Harvard’s Journal, beginning on page 18). • Readers’ support is the most important underpinning of this commitment to high- Your contribution underwrites the journalism you are reading now, the expanded coverage quality, editorially independent journalism on readers’ behalf. -
Possible Implications for Prader-Willi and Angelman Syndromes KARIN Buiting*, VALERIE GREGER*, BERNHARD H
Proc. Nadl. Acad. Sci. USA Vol. 89, pp. 5457-5461, June 1992 Medical Sciences A putative gene family in 15q11-13 and 16pll.2: Possible implications for Prader-Willi and Angelman syndromes KARIN BuITING*, VALERIE GREGER*, BERNHARD H. BROWNSTEINt, ROSE M. MOHRt, ION VOICULESCuO, ANDREAS WINTERPACHT§, BERNHARD ZABEL§, AND BERNHARD HORSTHEMKE*¶ *Institut for Humangenetik, Universititsklinikum Essen, 4300 Essen 1, Federal Republic of Germany; tCenter for Genetics in Medicine, Washington University, St. Louis, MO 63110; tInstitut fOr Humangenetik und Anthropologie, Universitit Freiburg, 7800 Freiburg, Federal Republic of Germany; and §Kinderklinik, UniversitAt Mainz, 6500 Mainz, Federal Republic of Germany Communicated by Victor A. McKusick, February 13, 1992 ABSTRACT The genetic defects in Prader-Willi syndrome of mouse A9 cells with lymphoblastoid cells from PWS (PWS) and Angelman syndrome (AS) map to 15q11-13. Using patient 168 (14) as described by Wigler et al. (15). The somatic microdissection, we have recently isolated several DNA probes cell hybrid mapping panels were kindly provided by G. Bruns for the critical region. Here we report that microclone MN7 (Boston) (panel I) and K. H. Grzeschik (Marburg) (panel II). detects multiple loci in 15q11-13 and 16pll.2. Eight yeast Genomic DNA Analysis. Genomic DNA was analyzed by artificial chromosome (YAC) clones, two genomic phage Southern blot hybridization as described (16). The final wash clones, and two placenta cDNA dones were isolated to analyze was usually at 650C in 150 mM sodium chloride/15 mM these loci in detail. Two ofthe YAC clones map to 16p. Six YAC sodium citrate (lx SSC) containing 0.1% SDS. The following clones and two genomic phage clones contain a total of four or probes were used: HuD2 (IGHDY2) (17), MN60 (D15564) five different MN7 copies, which are spread over a large (14), MN8 (D15S77) (14), MN47 (D15S78) (14), MN43 distance within 15q11-13. -
“Core” Genome: Pairwise Similarity Searches on Interspecific Genomic Data
Towards a “core” genome: pairwise similarity searches on interspecific genomic data. Bradly J. Alicea1, Marcela A. Carvallo-Pinto2, Jorge L.M. Rodrigues3 1 Department of Telecommunication, Information Studies, and Media, Michigan State University, East Lansing, MI 2 Department of Plant Biology, Michigan State University, East Lansing, MI 3 Center for Microbial Ecology, Michigan State University, East Lansing, MI. Keywords: Biological Variation, Genomics, Evolution, Bioinformatics Abstract The phenomenon of gene conservation is an interesting evolutionary problem related to speciation and adaptation. Conserved genes are acted upon in evolution in a way that preserves their function despite other structural and functional changes going on around them. The recent availability of whole-genomic data from closely related species allows us to test the hypothesis that a “core” genome present in a hypothetical common ancestor is inherited by all sister taxa. Furthermore, this “core” genome should serve essential functions such as genetic regulation and cellular repair. Whole-genome sequences from three strains of bacteria (Shewanella sp.) were used in this analysis. The open reading frames (ORFs) for each identified and putative gene were used for each genome. Reciprocal Blast searches were conducted on all three genomes, which distilled a list of thousands of genes to 68 genes that were identical across taxa. Based on functional annotation, these genes were identified as housekeeping genes, which confirmed the original hypothesis. This method could be used in eukaryotes as well, in particular the relationship between humans, chimps, and macaques. Introduction The study of gene conservation is an intriguing scientific problem that has consequences for understanding functional differences between species and biological variation in general. -
Title: Analysing the Drivers of Dietary Diversity, Diet Quality and Household Food Security in Developing Asia: Evidence from Urban Vietnam and Rural Bangladesh
Title: Analysing the drivers of dietary diversity, diet quality and household food security in developing Asia: Evidence from urban Vietnam and rural Bangladesh by Jesmin Ara Rupa Thesis submitted to the University of Adelaide in fulfilment of the requirements for the degree of Doctor of Philosophy The Centre for Global Food and Resources Faculty of the Professions The University of Adelaide February 2019 [Page left bank intentionally] ii Contents Title: Analysing the drivers of dietary diversity, diet quality and household food security in developing Asia: Evidence from urban Vietnam and rural Bangladesh ............................................ i Contents ................................................................................................................................................. iii List of Tables ....................................................................................................................................... viii List of Figures ......................................................................................................................................... x Abstract .................................................................................................................................................. xi Declaration ........................................................................................................................................... xiii Acknowledgements ............................................................................................................................. -
72 Hours in Harvard Square
HARVARD SQUARED HARVARD SQUARED ner Ran Duan, who has transformed half NOT YOUR of his parents’ Chinese restaurant. Tasty Sichuan dishes still reign across the din- 72 Hours in AVERAGE BAKERY ing areas, from dan dan noodles with pork and spinach and tofu-stued crepes with MILK BAR mushrooms to a whole fish with chili-mi- Harvard Square HARVARD SQUARE so sauce. Ask the wizard mixologists what dishes pair best with that night’s tantaliz- 2 Care packages & more at ing potions.($7.50-$22.95) A Harvard local business roundup milkbarstore.com For umami-packed Asian ramen and brown-rice bowls—and the addictive “Hawaiian-style burger” (a flat patty with crispy, salty onions, spicy mayo, and pine- apple relish)—run to Little Big Diner, in Newton. With only 15 seats, and a no- reservations poli- cy, show up to get SECTION ADVERTISING BUSINESS LOCAL SQUARE HARVARD on the wait list, and then browse in Newtonville Books, across the Looking for way, until the res- recommendations taurant calls you on where to (by phone) to a ta- eat, drink, and ble. ($8-$18) shop during Watertown’s be- Commencement loved and histor- week? Follow us ic Deluxe Town on Twitter at Diner serves the @harvardsqd. traditional all-day breakfast—the scrambled-eggs burrito, challah French toast, and sour-cream flap- jacks are the best around—along with beef BYGABRIELLA.CO and veggie burgers (with fresh-cut fries) Teaching the World® and a carb-rich turkey dinner. But there are plenty of lighter, wholesome items as well, like spinach and mushroom sal- ad, sautéed quinoa and vegetables, and a Middle Eastern sampler platter fit for two.