DOCSLIB.ORG

Ajoene, a Major Organosulfide Found in Crushed Garlic, Induces NAD(P

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Ajoene, a Major Organosulfide Found in Crushed Garlic, Induces NAD(P http://crossmark.crossref.org/dialog/?doi=10.15430/JCP.2019.24.2.112&domain=pdf&date_stamp=2019-6-30 JOURNAL OF CANCER PREVENTION https://doi.org/10.15430/JCP.2019.24.2.112 Vol. 24, No. 2, June 2019 pISSN 2288-3649ㆍeISSN 2288-3657 www.jcpjournal.org Ajoene, a Major Organosulfide Found in Crushed Original Garlic, Induces NAD(P)H:quinone Oxidoreductase Article Expression Through Nuclear Factor E2-related Factor-2 Activation in Human Breast Epithelial Cells Seung-Ju Cho1,*, Jae-Ha Ryu4, Young-Joon Surh1,2,3 1Tumor Microenvironment Global Core Research Center, College of Pharmacy, Seoul National University, 2Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and 3Cancer Research Institute, Seoul National University, 4College of Pharmacy, Sookmyung Women’s University, Seoul, Korea Background: NAD(P)H:quinone oxidoreductase-1 (NQO1) is a widely-distributed flavin adenine dinucleotide-dependent flavoprotein that promotes obligatory 2-electron reductions of quinones, quinoneimines, nitroaromatics, and azo dyes. This reduces quinone levels and thereby minimizes generation of excess reactive oxygen species (ROS) formed by redox cycling, and concurrent depletion of intracellular thiol pools. Ajoene is derived from crushed garlic. It is formed by a reaction involving two allicin molecules, and is composed of allyl sulfide and vinyl disulfide. Ajoene is present in two isomers, E- and Z-form. Methods: Expression of antioxidant enzymes and nuclear factor E2-related factor-2 (Nrf2) was measured by Western blot analysis. NQO1 promoter activity was assessed by the luciferase reporter gene assay. ROS accumulation was monitored by using the fluorescence-generating probe 2’,7’-dichlorofluorescein diacetate. The intracellular glutathione levels were measured by using a commercially available kit. Results: Z-ajoene significantly up-regulated the expression of representative antioxidant enzyme NQO1 in non-tumorigenic breast epithelial MCF-10A cells at non-toxic concentrations. Z-ajoene enhanced up-regulation and nuclear translocation of Nrf2, which plays a pivotal role in the induction of many genes encoding antioxidant enzymes and other cytoprotective proteins. Z-ajoene treatment also increased the activity of nqo1-promoter harboring antioxidant response element consensus sequences in MCF-10A cells. Silencing of Nrf2 by small interfering RNA abrogated ajoene-induced expression of NQO1. Z-ajoene activated extracellular signal-regulated kinase (ERK). Inhibition of ERK activation by U0126 abrogated ability of Z-ajoene to activate Nrf2 and to induce NQO1 expression. Intracellular ROS accumulation was observed after treatment with Z-ajoene, whereas the E-isoform was not effective. The inhibition of ROS by treatment with N-acetylcysteine, a radical scavenger, abrogated Z-ajoene-induced expression of NQO1 as well as activation of ERK and Nrf2, suggesting that Z-ajoene augments the Nrf2-dependent antioxidant defense via ROS generation and ERK activation. Conclusions: Z-ajoene induces NQO1 expression in MCF-10A cells through ROS-mediated activation of Nrf2. (J Cancer Prev 2019;24:112-122) Key Words: Ajoene, Garlic, Nuclear factor E2-related factor 2, NAD(P)H:quinone oxidoreductase 1, Extracellular signal-regulated kinase, Reactive oxygen species INTRODUCTION mitogen-activated protein kinases (MAPK) is considered to be a pivotal step in ROS-induced signaling pathways. It has been Reactive oxygen species (ROS) are recognized to play a key role shown that increased ROS production in leukemic cells leads to in cell signaling. At the cellular level, oxidant injury elicits a broad the activation of MAPK and cell death [2-5]. The MAPK pathways spectrum of responses ranging from proliferation to growth consist of three parallel kinase modules, that is, extracellular arrest, to senescence, and to cell death [1]. Activation of signal-regulating kinase (ERK1/2), c-Jun-N-terminal kinase (JNK), Received June 16, 2019, Revised June 24, 2019, Accepted June 25, 2019 Correspondence to: Young-Joon Surh E-mail: surh@ snu.ac.kr, ORCID: Young-Joon Surh, https://orcid.org/0000-0001-8310-1795 *Current affiliation: New Drug Development Center, Osong Medical Innovation Foundation, 123 Osongsaengmyeong-ro, Osong-eup, Heungdeok-gu, Cheongju 28160, Korea. Copyright © 2019 Korean Society of Cancer Prevention cc This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 112 Seung-Ju Cho, et al: Ajoene Induces NQO1 Expression Through Nrf2 Activation 113 and p38 MAPK pathways. In general, JNK and p38 MAPK MATERIALS AND METHODS activation is associated with apoptosis induction, whereas ERK 1. Chemicals and biological agents activation is cytoprotective [6]. Cells are equipped with a battery of cytoprotective enzymes, Crushed garlic bulb was incubated at the room temperature for which are expressed in response to oxidative stress to protect 12 hours, and at 37oC for additional 2 hours, and then extracted against ROS-induced damage of cellular macromolecules, such as with 80% ethanol. E- and Z-ajoene were purified by the repeated DNA, RNA, and proteins. These cytoprotective enzymes include chromatography and their structures were identified by NAD(P)H:quinine oxidoreductase-1 (NQO1), heme oxygenase-1 spectroscopic analysis. Dulbecco’s modified Eagle’s medium (HO-1), superoxide dismutase, glutathione (GSH)-S-transferase, (DMEM) and FBS were obtained from Gibco BRL (Grand Island, GSH peroxidase, glutamate cysteine ligase, uridine diphosphate NY, USA). Dithiothreitol (DTT), N-acetyl-L-cysteine (NAC), and glucuronosyltransferase, etc. [7,8]. Among them, NQO1 plays an anti-actin antibody were procured from Sigma Chemical Co. (St. important role in protection against endogenous and exogenous Louis, MO, USA). Primary antibodies for Nrf2 and NQO1 were quinines, thereby protecting the cell from undesired oxidative supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA). damage. Horseradish peroxidase-conjugated secondary antibodies were The proximal promoter regions of genes encoding many purchased from Zymed Laboratories Inc. (San Francisco, CA, antioxidant and cytoprotective enzymes including NQO1 harbor USA). The ECL chemiluninescent detection kit was obtained from a consensus sequence known as antioxidant response element Amersham Pharmacia Biotech (Buckinghamshire, UK). (ARE) or electrophile response element (EpRE), which is a Bicinchoninic acid protein assay reagent was a product of Pierce preferred target of the redox-sensitive transcription factor, Biotechnology (Rockfold, IL, USA). Mouse monoclonal lamin B1 nuclear factor E2-related factor-2 (Nrf2) [8]. Nrf2 is normally antibody, human specific Nrf2-siRNA (sense 5ˊ-AAGAGUAUGAG sequestered in the cytoplasm as an inactive complex with its CUGGAAAAACTT-3ˊ; antisense 5ˊ-GUUUUUCCAGCUCAUACUCU cytosolic repressor, named Kelch-like ECH associated protein 1 UTT-3ˊ), and StealthTM universal RNAi negative control duplexes, (Keap1). As an adaptive response to mild oxidative or electrophilic and 2ˊ,7ˊ-dichlorofluorescein diacetate (DCF-DA) were purchased insults, Nrf2 is dissociated from the inhibitory protein Keap1 and from Invitrogen (Carlsbad, CA, USA). Polyvinylidene difluoride translocates to nucleus where it binds to cis-acting ARE or EpRE, (PVDF) membrane was supplied from Gelman Laboratory (Ann leading to the transcriptional activation of antioxidant and Arbor, MI, USA). The inhibitor of extracellular signal-regulated cytoprotective genes [8,9]. kinase (ERK; U0126) was purchased from Tocris (Ellisville, MO, Ajoene is most stable and abundant in macerate of garlic. It is USA). present in two isomers, E- and Z-4,5,9-trithiadodeca-1,6,11-triene 2. Cells and culture 9-oxide. Ajoene, an unsaturated disulfide, is formed from a chemical reaction involving two allicin molecules. It functions as MCF-10A cells were purchased from the American Type an antioxidant by inhibiting the release of superoxide [10]. It also Culture Collection. The cells were suspended in DMEM/F12 has antithrombotic (anti-clotting) properties, which helps prevent medium supplemented with 10 g/mL insulin (bovine), 100 platelets from forming blood clots [11,12], potentially reducing ng/mL choleratoxin, 0.5 g/mL hydrocortisone, 20 ng/mL the risk of heart disease and stroke in humans. Ajoene is also recombinant human epidermal growth factor, 0.5 g/mL fungi known to have effective broad-spectrum of antimicrobial zone, 2 mmol/L L-glutamine, 100 g/mL penicillin/streptomycin/ (antibacterial and antifungal) properties [13,14]. Ajoene and fungi zone mixture, and 5% heat-inactivated horse serum and o several other organosulfur compounds from garlic have also been maintained at 37 C in 5% CO2. For isolation of embryonic reported to inhibit the proliferation and to induce apoptosis of fibroblasts, nrf2-null mice, in which the nrf2 gene is disrupted by human mammary, bladder, and skin tumor cell lines [15-18]. targeted gene knockout, were provided by Dr. Jeffery Johnson, Ajoene suppresses tumor cell growth by targeting the microtubule University of Wisconsin, Madison, WI, USA. The nrf2−/−, nrf2+/−, cytoskeleton of such cells [19]. and wild-type mice were maintained in the animal quarters in The present study explored the antioxidant effects of ajoene accordance with the university
Load more

Recommended publications
  • Garlic {Allium Sativum), and Garlic Constituents Allyl Alcohol and Diallyl Disulphide
    C a r d if f UNIVERSITY PR! FYSGOL QVRDv^ BINDING SERVICES Tel+44 (0)29 2087 4949 Fax+44 (0)29 20371921 e-mail [email protected] Cell death in the human pathogen Candida albicans: effects of garlic {Allium sativum), and garlic constituents allyl alcohol and diallyl disulphide. Thesis submitted for the degree of Doctor of Philosophy Katey M. Lemar BSc. (Hons) Cardiff School of Biosciences, Cardiff University, May 2005. UMI Number: U584725 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a complete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. Dissertation Publishing UMI U584725 Published by ProQuest LLC 2013. Copyright in the Dissertation held by the Author. Microform Edition © ProQuest LLC. All rights reserved. This work is protected against unauthorized copying under Title 17, United States Code. ProQuest LLC 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106-1346 Abstract Garlic extract is very complex, yielding a number of organic sulphur constituents that are thought to be responsible for its anticandidal properties. Many of these are now being investigated in an attempt to determine the mechanisms by which they act. The effects of fresh and freeze dried extracts of Allium sativum (garlic) on the physiology and morphology ofCandida albicans were compared. Inhibition of growth and loss of structural integrity was observed for both; fresh garlic extract (FGE) has a greater efficacy than garlic powder extract (GPE) as indicated both by its effects on morphology and inhibition of growth.
    [Show full text]
  • Dual Anti-Cholinesterase Activity of Ajoene by in Silico and in Vitrostudies
    [Downloaded free from http://www.phcogres.com on Tuesday, June 22, 2021, IP: 254.5.182.169] Pharmacogn. Res. SHORT COMMUNICATION A multifaceted peer reviewed journal in the field of Pharmacognosy and Natural Products www.phcogres.com | www.phcog.net Dual Anti-cholinesterase Activity of Ajoene by In silico and In vitro Studies Shivani Kumar, Sayan Chatterjee, Suresh Kumar University School of Biotechnology, Guru Gobind Singh Indraprastha University, New Delhi, India ABSTRACT The two major forms of cholinesterase enzymes found in the mammalian brain are acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). BuChE usually found mainly in glial cells and neuron in normal physiological condition, whereas AChE found near nerve synapse and axons, both are involved in the breakdown of acetylcholine (ACh) in the brain. The dual inhibition of these enzymes is considered as a promising strategy for the treatment of a neurological disorder such as Alzheimer’s disease, senile dementia, ataxia, and myasthenia gravis. The objective is to study the dual anticholinesterase activity of ajoene using in silico and in vitro methods. The anticholinesterase activity of ajoene was evaluated using Ellman’s assay, and molecular docking was performed on Schrödinger suite software. The present study demonstrated ajoene ([E, Z]‑4, 5, 9‑trithiadodeca‑1, 6, 11‑triene‑9‑oxide) inhibited both AChE and BuChE in a concentration‑dependent manner. The IC50 value of ajoene was 2.34 mM for AChE and 2.09 mM for BuChE. Kinetic studies showed mixed noncompetitive inhibition of AChE and uncompetitive inhibition of BuChE. Molecular docking studies revealed that ajoene interacts hydrophobically with catalytic residues of AChE Abbreviations Used: AChE: Acetylcholinesterease; while in case of BuChE the interaction is through noncatalytic binding BuChE: Butyrylcholinesterase; AD: Alzheimer’s disease; Ach: Acetylcholine; site residues.
    [Show full text]
  • Allicin and Related Compounds: Biosynthesis, Synthesis and Pharmacological Activity † Udc 547-305.2 : 635.262
    FACTA UNIVERSITATIS Series: Physics, Chemistry and Technology Vol. 9, No 1, 2011, pp. 9 - 20 DOI: 10.2298/FUPCT1101009I Review ALLICIN AND RELATED COMPOUNDS: BIOSYNTHESIS, SYNTHESIS AND PHARMACOLOGICAL ACTIVITY † UDC 547-305.2 : 635.262 Dušica P. Ilić , Vesna D. Nikolić, Ljubiša B. Nikolić, Mihajlo Z. Stanković, Ljiljana P. Stanojević, Milorad D. Cakić Faculty of Technology, Bulevar oslobođenja 124, 16000 Leskovac, Univesity of Nis, Serbia Abstract. In this review, the biosynthesis of allicin (allyl thiosulfinate) by enzymatic transformation of alliin and various methods of its synthesis with detailed investigation of mechanisms and kinetics are summarized. A convenient method is also described for determination of allicin stability and the utility of the inclusion complexes of this pharmacologically active agent with β-cyclodextrins in increasing its stability. Allicin is the initial precursor for the production of ajoene ((E)- and (Z)-4,5,9-trithiadodeca- 1,6,11-triene 9-oxides) and vinyldithiin (2-vinyl-4H-1,3-dithiin, and 3-vinyl-4H-1,2- dithiin), which are more stable and show various pharmacological effects. The mechanisms of allicin transformations to these compounds are given in detail. Finally, the data on the pharmacological effects of allicin and its transformation products, ajoene and vinyldithiin, are presented. Key words: allicin, ajoene, vinyldithiins, biosynthesis, synthesis, pharmacological activity 1. ALLICIN (ALLYL THIOSULFINATE) 1.1. Biosynthesis of allicin Under the influence of alliinase, allicin is produced by enzymatic transformation of alliin [(+)-(S)-allyl-L-cysteine-sulfoxide]. Alliin and alliinase are found in separate parts of garlic clove [1], therefore this chain reaction is initiated only after crushing the cells.
    [Show full text]
  • Allium Thiosulfinates: Chemistry, Biological Properties and Their Potential Utilization in Food Preservation
    Food ©2007 Global Science Books Allium Thiosulfinates: Chemistry, Biological Properties and their Potential Utilization in Food Preservation Noureddine Benkeblia1,2* • Virginia Lanzotti3 1 Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University, Ebetsu, Hokkaido, 060-8501, Japan 2 Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan 3 Department of STAAM, University of Molise, 86100 Campobasso, Italy Corresponding author : * [email protected], [email protected] ABSTRACT Onion (Allium cepa L.), garlic (Allium sativum L.) and other edible Allium are among the oldest cultivated plants, and are used for multiple purposes. They are a rich source of several phytonutrients, and recognized to have significant and wide biological activities. These biological activities are related to the thiosulfinates, volatile sulfur compounds which are responsible for the pungency of these vegetables. The thiosulfinates or alkane(ene) thial-S-oxide are formed by the action of the enzyme alliinase (E.C. 4.4.1.4) from their respective S-alk(en)yl cysteine sulfoxides. However, depending on the Allium species, and under differing conditions, thiosulfinates can decompose to form additional sulfur constituents, including diallyl, methyl allyl, and diethyl mono-, di-, tri-, tetra-, penta-, and hexasulfides, vinyldithiins, and (E)- and (Z)-ajoene. With increasing interest into the utilization of natural biologically active compounds and the development of specific packaging, mainly active packaging, the thiosulfinates aroused much interest for the improvement of shelf-life and safety of perishable foods, and their potency as food preservatives and substitutes for chemicals. This review examines the nature and the biological activities of Allium thiosulfinates and their potential values as food preservatives in food preservation and shelf- life extension.
    [Show full text]
  • Analytical Methods for Bioactive Sulfur Compounds in Allium: an Integrated Review and Future Directions
    Accepted Manuscript Title: Analytical methods for bioactive sulfur compounds in Allium: An integrated review and future directions Author: Daniela A. Ramirez Daniela A. Locatelli Roxana E. Gonzalez´ Pablo F. Cavagnaro Alejandra B. Camargo PII: S0889-1575(16)30162-4 DOI: http://dx.doi.org/doi:10.1016/j.jfca.2016.09.012 Reference: YJFCA 2765 To appear in: Received date: 31-5-2016 Revised date: 7-9-2016 Accepted date: 28-9-2016 Please cite this article as: Ramirez, Daniela A., Locatelli, Daniela A., Gonzalez,´ Roxana E., Cavagnaro, Pablo F., & Camargo, Alejandra B., Analytical methods for bioactive sulfur compounds in Allium: An integrated review and future directions.Journal of Food Composition and Analysis http://dx.doi.org/10.1016/j.jfca.2016.09.012 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1 STUDY REVIEW Analytical methods for bioactive sulfur compounds in Allium: an integrated review and future directions Daniela A. Ramirez a, Daniela A. Locatelli b, Roxana E. González c,e, Pablo F. Cavagnaro c,f, and Alejandra B. Camargo b,d,e*. a IANIGLA, CONICET, Avenida Ruiz Leal s/n, 5500, Parque General San Martín, Mendoza, Argentinab Laboratorio de Cromatografía para Agroalimentos, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo.
    [Show full text]
  • Antioxidant Action and Therapeutic Efficacy of Allium Sativum L
    Molecules 2013, 18, 690-700; doi:10.3390/molecules18010690 OPEN ACCESS molecules ISSN 1420-3049 www.mdpi.com/journal/molecules Review Antioxidant Action and Therapeutic Efficacy of Allium sativum L. Anna Capasso Department of Pharmacy, University of Salerno, Via Ponte don Melillo, Fisciano (Salerno) 84084, Italy; E-Mail: [email protected]; Tel.: +39-089-969-744; Fax: +39-089-969-602 Received: 23 November 2012; in revised form: 14 December 2012 / Accepted: 28 December 2012 / Published: 4 January 2013 Abstract: Allium sativum (L.) is rich in antioxidants which help destroy free radicals particles that can damage cell membranes and DNA, and may contribute to the aging process as well as the development of a number of conditions, including heart disease and cancer. Antioxidants neutralize free radicals and may reduce or even help prevent some of the damage they cause over time. The antioxidant activity of fresh Allium sativum L. (garlic) is well known and is mainly due to unstable and irritating organosulphur compounds. Fresh garlic extracted over a prolonged period (up to 20 months) produces odourless aged garlic extract (AGE) containing stable and water soluble organosulphur compounds that prevent oxidative damage by scavenging free radicals. The aim of this review was to understand the mechanism of antioxidant action and therapeutic efficacy of garlic. Keywords: Allium; garlic; antioxidant activity 1. Introduction The free radical theory is based on the evidence that living organisms (aerobes) produce oxygen-centered free radicals, capable of inducing irreversible damage to biological structures. These are formed inside body cells when oxygen is used in metabolic processes in order to produce energy.
    [Show full text]
  • Role of Garlic (Allium. Sativum L.) in Human and Plant Diseases
    Indian Journal of Experim cntal Biology Vol. 39. Apri l 200 1. pp. 310-322 Review Article Role of garlic (Allium. sativum L.) in human and plant diseases UP Singh ', B Prilhiviraj ', B K Sarma ', Mandavi Singh2 & A B Rai 'Departmen t of Mycology and Pl ant Pathology. Institutc of Agri cultural Scienccs. Banaras Hindu Universit y, Varan as i 221005, India Fax: 9 1-542-3 17074. E mail: ups @ banaras. crn et.in !Dcpart mcnt of Anatomy. In sti tute of Mcdical Scicnces, I3anaras Hindu Uni ve rsity, Va r3nasi 22 1005. India, JDepartment of Mcd icinal Chemistry, In stitutc of Medical Scienccs. Banaras Hindu Univcrsity, Varanasi 221005. India A n:surgcncc of intcrest in garlic due to recent rcvelations of its bcneficial cffec ts in th c treatmcnt of various hum an and plan t diseascs and also due to validation of claims made in traditional systems of medici nc has resulted a plcth ora of publi ca ti ons on diffcrcnt a pects of garlic in reCC I1l years. Chcmical constituents of garlic and their variati ons on the mcth ods of isolation havc been discussed in the prcsent rev iew. Effect of garlic and it s constituents aga inst various human anel plant pathogenic and sap roph yt ic mi croorganisms has also been reviewcd. Garlic (A lliulIl sativulII L.) is used worldwide as a view of the variety of bioactivities th at garlic and its l food additive, spi ce and medicine. In Ayurveda , it various preparations exhibit, it is considered has been described as a gastric sti mu lant.
    [Show full text]
  • Author Section
    AUTHOR SECTION patients who may be treated in the outpatient setting with oral M antimicrobials from patients in whom hospitalization and parenteral therapy is appropriate. Over the past decade, dramatic escalation in antimicrobial resistance among common respiratory pathogens poses Macartney K.K. et al. Nosocomial respiratory syncytial virus infections: the obstacles to antibiotic choices.We review the microbiology of com- cost-effectiveness and cost-benefit of infection control. Pediatrics. 2000; munity-acquired pneumonia, and the therapeutic strategies that are 106(3) : 520-6.p Abstract: OBJECTIVE:To determine the cost- clinically and cost effective. effectiveness and cost-benefit of an infection control program to reduce nosocomial respiratory syncytial virus (RSV) transmission in Lyon W.R. et al. A role for trigger factor and an rgg-like regulator in the tran- a large pediatric hospital. DESIGN: RSV nosocomial infection (NI) scription, secretion and processing of the cysteine proteinase of Streptococcus was studied for 8 years, before and after intervention with a target- pyogenes. EMBO J. 1998; 17(21) : 6263-75.p Abstract: The abili- ed infection control program.The cost-effectiveness of the interven- ty of numerous microorganisms to cause disease relies upon the tion was calculated, and cost-benefit was estimated by a case-control highly regulated expression of secreted proteinases. In this study, comparison. SETTING: Children’s Hospital of Philadelphia, a 304- mutagenesis with a novel derivative of Tn4001 was used to identify bed pediatric hospital. PATIENTS: All inpatients with RSV infec- genes required for the expression of the secreted cysteine proteinase tion, both community- and hospital-acquired. INTERVENTION: (SCP) of the pathogenic Gram-positive bacterium Streptococcus Consisted of early recognition of patients with respiratory symp- pyogenes.
    [Show full text]
  • Bioactive Substances of Garlic and Their Role in Dietoprophylaxis and Dietotherapy
    ROCZN. PZH 2007, 58, NR 1, 41-46 FRANCISZEK ŚWIDERSKI, MONIKA DĄBROWSKA, ANNA RUSACZONEK, BOŻENA WASZKIEWICZ-ROBAK BIOACTIVE SUBSTANCES OF GARLIC AND THEIR ROLE IN DIETOPROPHYLAXIS AND DIETOTHERAPY SUBSTANCJE BIOAKTYWNE CZOSNKU I ICH ROLA W DIETOPROFILAKTYCE I DIETOTERAPII Department of Functional Foods and Commodity Warsaw Agricultural University 02-776 Warsaw, Nowoursynowska 159C, Poland e-mail: [email protected] Head: prof. dr hab. F. Świderski This work has aimed to characterise the most important bioactive substances of garlic, its preparations and describe in detail the role of garlic in dietoprophylaxis and dietotherapy. Key words: garlic, bioactive substances, dietotherapy, dietoprophylaxis Słowa kluczowe: czosnek, substancje biologicznie aktywne, dietoterapia, dietoprofilaktyka Garlic (Allium sativum) originating from Middle Asia belongs to the group of plants of nutritious, medicinal and pro-health activity. Garlic health benefits result from the content of over 200 biologically active substances. Fresh garlic bulbs contain about 60 % of water, 32 % of carbohydrates and 6,5 % of fiber. Vitamin C is present in the greatest amount (about 31 mg in 100 g of product). Garlic also supplies vitamins from A, B1, B2 and PP group. Among mineral ingredients, potassium is present in the highest concentra- tion, whilst iron, magnesium and phosphorus in a slightly lower concentration. Garlic contains considerable amount of biologically active substances. Allicin, the by - product of disintegration of biologically inactive alliin, belongs to the most important biologi- cally active substances of characteristic odour. Alliin is allylcysteine sulfoxide, crystal- line, colourless, odourless and without antibiotic properties, but it is present in whole, unbruised garlic cloves [11, 13]. During crushing, mechanical damage of garlic bulb, the enzyme alliinase (lyase) is pro- duced.
    [Show full text]
  • Bioactive Compounds and Biological Functions of Garlic (Allium Sativum L.)
    foods Review Bioactive Compounds and Biological Functions of Garlic (Allium sativum L.) Ao Shang 1, Shi-Yu Cao 1, Xiao-Yu Xu 1, Ren-You Gan 2,3,* , Guo-Yi Tang 1, Harold Corke 2, Vuyo Mavumengwana 4 and Hua-Bin Li 1,* 1 Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China 2 Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China 3 Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610213, China 4 DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, US/SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town 8000, South Africa * Correspondence: [email protected] (R.-Y.G.); [email protected] (H.-B.L.); Tel.: +86-21-3420-8517 (R.-Y.G.); +86-20-873-323-91 (H.-B.L.) Received: 27 May 2019; Accepted: 4 July 2019; Published: 5 July 2019 Abstract: Garlic (Allium sativum L.) is a widely consumed spice in the world. Garlic contains diverse bioactive compounds, such as allicin, alliin, diallyl sulfide, diallyl disulfide, diallyl trisulfide, ajoene, and S-allyl-cysteine. Substantial studies have shown that garlic and its bioactive constituents exhibit antioxidant, anti-inflammatory, antibacterial, antifungal, immunomodulatory, cardiovascular protective, anticancer, hepatoprotective, digestive system protective, anti-diabetic, anti-obesity, neuroprotective, and renal protective properties. In this review, the main bioactive compounds and important biological functions of garlic are summarized, highlighting and discussing the relevant mechanisms of actions.
    [Show full text]
  • Chemical Composition and Bioactive Compounds of Garlic (Allium Sativum L.) As Affected by Pre- and Post-Harvest Conditions: a Review
    Chemical composition and bioactive compounds of garlic (Allium sativum L.) as affected by pre- and post-harvest conditions: A review Natália Martins2, Spyridon Petropoulos1*, Isabel C.F.R. Ferreira2* 1Department of Agriculture Crop Production and Rural Environment, University of Thessaly, Fytokou Str, 38446, Nea Ionia, Magnesia, Greece. 2Mountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, 1172, 5301-855 Bragança, Portugal *Address correspondence to: Spyridon A. Petropoulos, University of Thessaly, School of Agricultural Sciences, Fytokou Street, 38446, N. Ionia, Magnissia, Greece. Email address: [email protected]; and Isabel C.F.R. Ferreira, Polytechnic Institute of Bragança, Campus de Santa Apolónia, 1172, 5301-855 Bragança, Portugal. Email address: [email protected] 1 ABSTRACT Garlic (Allium sativum L.) is considered one of the twenty most important vegetables, with various uses throughout the world, either as raw vegetable for culinary purposes, as also an ingredient in traditional and modern medicine. Further, it has been also proposed as one of the richest sources of total phenolic compounds among the usually consumed vegetables, whereas highly ranked regarding its contribution of phenolic compounds in human diet. This review aims to examine all the aspects related with garlic chemical composition and quality, focusing on bioactive properties of the final produce. A particular emphasis is given on the organosulfur compounds contents, since they exert a great contribution to the effective bioactive properties of garlic, including its derived products. The determinant effects of pre-harvest (genotype and various cultivation practices) and post-harvest conditions (storage conditions and processing treatments) on chemical composition and, consequently bioactive potential of garlic are also discussed.
    [Show full text]
  • (Allium Sativum L.): a Review
    nutrients Review Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review 1,2, , 1, 2 Gaber El-Saber Batiha * y , Amany Magdy Beshbishy y, Lamiaa G. Wasef , Yaser H. A. Elewa 3,4, Ahmed A. Al-Sagan 5 , Mohamed E. Abd El-Hack 6 , Ayman E. Taha 7 , Yasmina M. Abd-Elhakim 8 and Hari Prasad Devkota 9 1 National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, Obihiro, Hokkaido 080-8555, Japan; [email protected] 2 Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt; [email protected] 3 Department of Histology and Cytology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt; [email protected] 4 Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan 5 King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi Arabia; [email protected] 6 Department of Poultry, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt; [email protected] 7 Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Edfina 22578, Egypt; [email protected] 8 Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt; [email protected] 9 Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-Honmachi, Chuo-ku, Kumamoto City, Kumamoto, 862-0973, Japan; [email protected] * Correspondence: [email protected]; Tel.: +20-45-271-6024; Fax: +20-45-271-6024 These two authors share the first authorship.
    [Show full text]