RESEARCH ARTICLE A New Method for Xenogeneic Bone Graft Deproteinization: Comparative Study of Radius Defects in a Rabbit Model Pengfei Lei1, Rongxin Sun2, Long Wang1, Jialin Zhou3, Lifei Wan4, Tianjian Zhou5, Yihe Hu1* 1 Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, China, 2 Department of Orthopedics, The Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi, China, 3 Department of Orthopedics, Thoracic hospital of Hunan province, Changsha, China, 4 Department of Orthopedics, Ningxiang People’s Hospital, Ningxiang, China, 5 Department of Orthopedics, The First People’s Hospital of Shenzhen, Shenzhen, China *
[email protected] Abstract OPEN ACCESS Background and Objectives Citation: Lei P, Sun R, Wang L, Zhou J, Wan L, Zhou T, et al. (2015) A New Method for Xenogeneic Bone Deproteinization is an indispensable process for the elimination of antigenicity in xenograft Graft Deproteinization: Comparative Study of Radius bones. However, the hydrogen peroxide (H2O2) deproteinized xenograft, which is com- Defects in a Rabbit Model. PLoS ONE 10(12): monly used to repair bone defect, exhibits limited osteoinduction activity. The present study e0146005. doi:10.1371/journal.pone.0146005 was designed to develop a new method for deproteinization and compare the osteogenic Editor: James Cray, Medical University of South capacities of new pepsin deproteinized xenograft bones with those of conventional H2O2 Carolina, UNITED STATES deproteinized ones. Received: March 15, 2015 Accepted: December 12, 2015 Methods Published: December 31, 2015 Bones were deproteinized in H2O2 or pepsin for 8 hours. The morphologies were compared Copyright: © 2015 Lei et al. This is an open access by HE staining. The content of protein and collagen I were measured by the Kjeldahl method article distributed under the terms of the Creative and HPLC-MS, respectively.