Date : June 18, 2018

CERTIFICATE OF ANALYSIS – TOTAL FATTY ACIDS METHYL ESTERS (FAMES)

SAMPLE IDENTIFICATION

Internal code : 18F14-INT1-2-CC Customer identification : Supplément de chanvre Type : & Source : Cannabis sativa Customer : Interne

ANALYSIS

Method: Simultaneous hydrolysis and methylation of oil sample using a mixture of heptane/methanol/ toluene/1,2-dimethoxypropane/sulfuric acid. Injection of the upper phase on GC-FID on a HP-88 column for quantification using the method FAMES, with identification of the methyl esters against standards. Analyst : Alexis St-Gelais, M. Sc., chimiste Analysis date : June 18, 2018

Checked and approved by :

Alexis St-Gelais, M. Sc., chimiste 2013-174

Note: This report may not be published, including online, without the written consent from Laboratoire PhytoChemia.

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CONCLUSION

The profile is in line with expectations for hemp seed oil.

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ANALYSIS DATA

Fatty acids Shorthand R.T R.I % Type Common name Systematic name formula Tetradecanoic acid C14:0 10.16 1400 0.06 SFA Pentadecanoic acid C15:0 11.36 1500 0.02 SFA Hexadecanoic acid C16:0 12.82 1600 6.84 SFA Hypogeic acid (7 Z)-Hexadecenoic acid C16:1 n-9 c 13.73 1653 0.03 MUFA (9 Z)-Hexadecenoic acid C16:1 n-7 c 13.91 1663 0.10 MUFA Heptadecanoic acid C17:0 14.60 1701 0.06 SFA cis -9-Heptadecenoic (9 Z)-Heptadecenoic acid C17:1 n-8 c 15.88 1758 0.04 MUFA acid Octadecanoic acid C18:0 16.88 1801 3.65 SFA C18:1 isomer Octadecenoic acid C18:1 17.83 1837 0.89 MUFA C18:1 isomer Octadecenoic acid C18:1 17.90 1840 1.20 MUFA C18:1 isomer Octadecenoic acid C18:1 17.97 1842 1.08 MUFA C18:1 isomer Octadecenoic acid C18:1 18.10 1847 0.63 MUFA (9 Z)-Octadecenoic acid C18:1 n-9 c 18.31 1855 9.49 MUFA cis - (11 Z)-Octadecenoic acid C18:1 n-7 c 18.53 1863 1.09 MUFA (9 E,12 E)-Octadecadienoic C18:2 n-6 tt 19.50 1899 0.34 PUFA acid Nonadecylic acid Nonadecanoic acid C19:0 19.68 1905 0.71 SFA cis ,trans -9,12- (9 Z,12 E)-Octadecadienoic C18:2 n-6 ct 20.09 1920 1.56 PUFA Octadecadienoic acid acid trans ,cis -9,12- (9 E,12 Z)-Octadecadienoic C18:2 n-6 tc 20.33 1928 1.27 PUFA Octadecadienoic acid acid (9 Z,12 Z)-Octadecadienoic C18:2 n-6 cc 20.69 1941 46.59 PUFA acid (6 Z,9 Z,12 Z)-Octadecatrienoic γ-Linolenic acid C18:3 n-6 ccc 22.35 1998 3.93 PUFA acid Eicosanoic acid C20:0 22.46 2002 1.05 SFA (9 Z,12 Z,15 Z)- α-Linolenic acid C18:3 n-3 ccc 23.53 2036 12.76 PUFA Octadecatrienoic acid Gondoic acid (11 Z)-Eicosenoic acid C20:1 n-9 c 24.10 2054 0.52 MUFA (13 Z)-Eicosenoic acid C20:1 n-7 c 24.40 2064 0.09 MUFA (6 Z,9 Z,12 Z,15 Z)- C18:4 n-3 cccc 25.38 2096 0.96 PUFA Octadecatetraenoic acid Heneicosanoic acid C21:0 25.63 2104 0.13 SFA cis ,cis -11,14- (11 Z,14 Z)-Eicosadienoic acid C20:2 n-6 cc 26.77 2142 0.09 PUFA Eicosadienoic acid Docosanoic acid C22:0 28.53 2202 0.47 SFA (13 Z)-Docosenoic acid C22:1 n-9 c 29.86 2257 0.03 MUFA Tricosanoic acid C23:0 30.88 2302 0.05 SFA Tetracosanoic acid C24:0 32.84 2400 0.26 SFA Total SFA: 13.28% Total PUFA: 64.67% Total identified 95.98% Total MUFA: 15.60% Note: no correction factor was applied R.T.: Retention time (minutes) R.I.: Retention index SFA: Saturated fatty acid

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PUFA: Polyunsaturated fatty acid MUFA: Monounsaturated fatty acid

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