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G:\YNL\Current Issue\Zy14632.Wpd ISSN 0513-5222 Official Publication of the International Commission on Yeasts of the International Union of Microbiological Societies (IUMS) DECEMBER 2014 Volume LXIII, Number II Marc-André Lachance, Editor University of Western Ontario, London, Ontario, Canada N6A 5B7 <[email protected] > http://www.uwo.ca/biology/YeastNewsletter/Index.html Associate Editors Peter Biely Patrizia Romano Kyria Boundy-Mills Institute of Chemistry Dipartimento di Biologia, Herman J. Phaff Culture Slovak Academy of Sciences Difesa e Biotecnologie Collection Dúbravská cesta 9, 842 3 Agro-Forestali Department of Food Science 8 Bratislava, Slovakia Università della Basilicata, and Technology Via Nazario Sauro, 85, 85100 University of California Davis Potenza, Italy Davis California 95616-5224 WI Golubev, Puschino, Russia . 26 H Prillinger, Großmeiseldorf, Austria . 34 GI Naumov and E.S. Naumova, S Casaregola, Thiverval-Grignon, France . 35 Moscow, Russia ..................... 26 B Gibson, VTT, finland .................. 36 AK Adya, Dundee, Scotland . 27 A Yurkov, Braunschweig, Germany . 41 NA Khan, New York, New York . 28 MA Lachance, London, Ontario, Canada . 44 E Breierová, Bratislava, Slovakia . 28 International Commission on Yeasts . 45 D Kregiel, Lodz, Poland ................. 29 Recent meeting......................... 46 C Hittinger, Madison, Wisconsin, USA . 31 Forthcoming Meetings ................... 47 H Lee, Guelph, Ontario, Canada . 33 Fifty Years Ago ........................ 48 S Nasr, Tehran, Iran ..................... 34 I wish our readers a happy and scientifically prosperous New Year! M.A. Lachance, Editor I Russian Collection of Microorganisms (VKM), Institute for Biochemistry and Physiology of Microorganisms, Pushchino, 142290, Russia. Communicated by WI Golubev <[email protected] > http://www.vkm.ru . Recent publications. 1 Golubev WI, Pfeiffer I 2014 A Study of Schizoblastosporion starkeyi-henricii isolates from northern and southern hemispheres of the Earth. Microbiology 83(5):661–665. The species Schizoblastosporion starkeyi-henricii is Analyses of the D1/D2 region of the 26S rDNA and common in peats and peaty soils. Fifteen strains of this internal transcribed spacers (ITS) confirm that these species isolated from Europe and East Falkland Island strains and also Asian isolates are conspecific. Our were found homogenous in cultural, morphological and results show that ecological factors but not geographic physiological characters, with the exception of one distances determine the global distribution of yeast strain that was sensitive to elevated osmotic pressure. fungi. 2 Golubev WI 2015 Intraspecific and intrageneric antagonistic activity in Wickerhamomyces anomalus . Microbiology 84 (in press) . Strains of Wickerhamomyces anomalus were which fell into five subgroups by activity against species examined for antagonistic activity. They include of their own genus and Candida spp. related to nomenclatural types of species which names are Wickerhamomyces phylogenetically. For the most part, considered now as synonyms. More than 70% of strains antibiotic agents are mycocins that are different in their exhibited antibiotic activity . Among them three groups physico-chemical properties. were revealed by activity against W. anomalus strains II State Scientific-Research Institute for Genetics and Selection of Industrial Microorganisms (GosNIIgenetika), I-Dorozhnyi 1, Moscow 117545, Russia. Communicated by GI Naumov and ES Naumova < [email protected] >. We are grateful to Tatiana Tanaschuk (INMIV “Magarach”, Yalta, Crimea) and Kyria Boundy-Mills (Herman J. Phaff Yeast Culture Collection at University of California, Davis) for visiting their labs in June 2014 and September 2014. We were glad to accept in our lab Taiwanese colleagues Lee Ching-Fu (National Hsinchu University of Education) and Wang Pin-Han (Tonghai University) during short visit in June 2014. The following are papers for 2014 or submitted. 1 Naumova ES, Sadykova AZh, Martynenko NN, Naumov GI 2014 Molecular polymorphism of β-fructosidase SUC genes in the Saccharomyces yeasts. Molecular Biology (Moscow), 48 (4):573–582. © Pleiades Publishing, Ltd. 2 Naumov GI, Naumova ES, Glushakova AM, Kachalkin AV, Chernov IYu 2014 Finding of dairy yeasts Kluyveromyces lactis var. lactis in natural habitats. Microbiology (Moscow) 83 (6): 782–786. © Pleiades Publishing, Ltd. Well-known yeasts Kluyveromyces lactis var. lactis , and molecular markers (nucleotide sequence of the 5.8S- which are usually associated with dairy products, were ITS rDNA fragment, pulsed-field gel electrophoresis, discovered in nature: in woodland park soil under and Southern hybridization of chromosomal DNA with Impatiens glandulifera Royle plants. Reliable the LAC4 probe). Ecology of Kl. lactis var. lactis is identification of the yeasts was carried out using discussed. physiological criteria (lactose and maltose utilization) 3 Naumov GI, Kondratieva VI, Naumova ES 2015 Hybrid sterility of the yeast Schizosaccharomyces pombe : genetic genus and many species in statu nascendi? Microbiology (Moscow). 84 (1) (in press). 26 A phenomenon of ascospore death was observed in biological data indicated existence of at least geograph- a number of Schizosaccharomyces pombe interstrain ical divergence of the genomes in Sch. pombe hybrids. Meiotic recombination of the control parental populations. Classification of the genus, species, and auxotrophic markers was, however, observed in a varieties of these yeasts is discussed. random sample of ascospores. Genetic and molecular 4 Naumov GI 2015 Hyperproduction of invertase can confer inulin fermentation in selection strains of Saccharomyces cerevisiae. Microbiology (Moscow). (in press). There are several recent publications on selection allow to consider that the hyperproduction of inverstase, strains of Saccharomyces cerevisiae capable to ferment a common enzyme of Saccharomyces cerevisiae yeasts, inulin; it is the authors’ opinion that such strains possess takes place in selection strains. inulinase. The review summarizes literature data, which 5 Naumov GI 2015 The yeast Komagataella – genetic genus in accordance with interspecies hybridization. Microbiology (Moscow). (submitted) Using induced complementary auxotrophic mutants The data obtained suggest that the genus Komagataella , and selective growth of prototrophic hybrids on minimal established earlier by phylogenetic analysis, corresponds medium, hybridization of the type strain of Komagata- well to the concept of genetic genus in ascomycetous ella kurtzmanii VKPM Y-727 with the type strains of fungi. According to the concept, the genetic genus is a K. pastoris VKPM Y-3262, K. phaffii NRRL Y-7556, group of hybridized species having a common mating K. populi NRRL YB-455, K. pseudopastoris NRRL type system. Employment of the concept of genetic Y-27603 and K. ulmi NRRL YB-407 was demonstrated. genus to different yeast genera is discussed. 6 Naumov GI, Naumova ES, Lee Ch-Fu 2015 Towards reinstatement of the yeast genus Zygowilliopsis Kudriavzev (1960). Microbiology (Moscow). (submitted) Experimental data on genetic molecular classif- fukushimae . Biogeography, ecology and killer activity of ication and identification of the yeast genus Z. californica yeasts is considered. Hetero-geneity of the Zygowilliopsis are summarized. The genus is represent- taxonomic genus Barnettozyma Kurtzman et al. (2008) ed by at least five biological species and three varieties: and necessity of its revision are discussed. Z. californica : var. californica , var. dimennae and var. III Division of Forensic and Computing Sciences, School of Science, Engineering and Technology, Bell Street, Dundee DD1 1HG, Scotland, UK. Communicated by Ashok K Adya <[email protected]>. We are grateful to Dr. Forbes Wardrop, Lallemand Inc., Montreal, Canada, for making it possible for us to visit Lallemand labs in Montreal in May/June 2014 for discussions and collaborative work. The abstract of a recently published paper follows. An open access PDF is available at: http://www.bluepenjournals.org/ijambr/pdf/2014/November/Nayyar_et_al.pdf 1 Ashima Nayyar, Graeme Walker, Elisabetta Canetta, Forbes Wardrop, Ashok K Adya 2014 Cell surface properties and flocculation behaviour of industrial strains of Saccharomyces cerevisiae. Cellular adhesion properties of yeasts depend on the yeast strain was found to be the most flocculent. Very characteristics of the outer layer of the cell wall. In this little flocculation was observed during the lag and study, the flocculation behaviour of four industrial logarithmic phases of growth (1-15%), while during the strains of Saccharomyces cerevisiae used for production early and late stationary phases, different strains of beer, champagne, wine and fuel alcohol was exhibited variable flocculation patterns. Cell surface evaluated; their flocculation abilities being, 42.5%, hydrophobicity (assayed using HMA and MATS) and 14.8%, 13.8% and 11.6%, respectively. The brewing surface charge (assayed by Alcian Blue dye retention) 27 played important roles in dictating flocculation Champagne (33, 10.5) > fuel alcohol (22.4, 7.4) > wine behaviour in different yeast strains, as did the yeast (20.5, 2.7). Our findings provide new insight into yeast growth phase. Percentage hydrophobicity index (HI) and cell surface properties and how these relate to % hydrophobicity of the four strains followed, behavioural characteristics of yeasts employed in respectively the same order, viz Beer (66.6, 21.5) > industrial fermentations. IV Biology Department,
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