D Subgroup Phytoplasma Associated with Parsley Witches’

Total Page:16

File Type:pdf, Size:1020Kb

D Subgroup Phytoplasma Associated with Parsley Witches’ J Phytopathol ORIGINAL ARTICLE Occurrence and Characterization of a 16SrII-D Subgroup Phytoplasma Associated with Parsley Witches’ Broom Disease in Iran Mohammad Salehi1, Seyyed Alireza Esmailzadeh Hosseini2, Elham Salehi1 and Assunta Bertaccini3 1 Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran 2 Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran 3 Department of Agricultural Sciences, Plant Pathology, Alma Mater Studiorum - University of Bologna, Bologna, Italy Keywords Abstract 16S rRNA, ‘Candidatus Phytoplasma australasia’, dodder and graft transmission, During 2010–2013 surveys for the presence of phytoplasma diseases in molecular analysis, parsley diseases Yazd province (Iran), a parsley witches’ broom (PrWB) disease was observed. Characteristic symptoms were excessive development of short Correspondence spindly shoots from crown buds, little leaf, yellowing, witches’ broom, M. Salehi, Plant Protection Research stunting, flower virescence and phyllody. The disease causative agent was Department, Fars Agricultural and Natural dodder transmitted from symptomatic parsley to periwinkle and from Resources Research and Education Center, AREEO, Shiraz, Iran. periwinkle to periwinkle by grafting inducing phytoplasma-type symp- E-mail: [email protected] toms. Expected length DNA fragments of nearly 1800 and 1250 bp were, respectively, amplified from naturally infected parsley and experimentally Received: June 12, 2016; accepted: August 9, inoculated periwinkle plants in direct polymerase chain reaction (PCR) 2016. using phytoplasma primer pair P1/P7 or nested PCR using the same pri- mer pair followed by R16F2n/R16R2 primers. Restriction fragment length doi: 10.1111/jph.12520 polymorphism and phylogenetic analyses of 16S rRNA gene sequences showed that the phytoplasma associated with PrWB disease in Yazd pro- vince belong to 16SrII-D phytoplasma subgroup. This is the first report of association of a 16SrII-related phytoplasma with PrWB disease in Iran. European, Middle East and American cuisine (Athar Introduction et al. 1999; Mozafarian 2007a,b). The parsley impor- Phytoplasmas are cell wall-less phloem inhabiting tance is attributed to its high vitamin content (mainly bacteria affecting more than 1000 plant species vitamin C), antioxidants and some mineral elements including many economically important fruits, veg- such as iron and volatile oils that play an important etables, cereals and trees. Characteristic symptoms role in the pharmaceutical and food industries (Lopez associated with phytoplasma infection include yellow- et al. 1999; Sidra et al. 2014). The Iranian production ing, little leaf, flower virescence, phyllody, prolifera- of fresh vegetables including parsley is 3 646 570 tons tion and sterility, witches’ broom and dwarfing (Lee (FAOSTAT 2013). et al. 2000; Bertaccini et al. 2014). Phytoplasmas are There is only one report available of aster yellows vectored by leafhoppers, planthoppers and psyllids phytoplasmas in parsley with yellows symptoms in (Weintraub and Beanland 2006); however, they can Canada (Khadhair et al. 1998) and one report on the also be transmitted by dodder, grafting and vegetative presence of Spiroplasma citri in asymptomatic parsley propagation (Lee and Davis 1992). (Gera et al. 2011). During 2010–2013 surveys for the Vegetables are infected by phytoplasmas belonging presence of phytoplasma diseases in Yazd province to different 16S ribosomal RNA groups worldwide (Iran) parsley plants showing excessive development (Lee et al. 2003, 2004). Parsley [Petroselinum crispum of short spindly shoots from crown buds, little leaf, (Mill.) Fuss, 1866, Apiaceae] is a biennial herb native yellowing, stunting, flower virescence and phyllody to the Mediterranean region and is widely used in were observed, the main common symptom was the Ó 2016 Blackwell Verlag GmbH 1 Parsley witches’ broom phytoplasma M. Salehi et al. witches’ broom, and therefore, the disease was named of disease symptoms and polymerase chain reaction parsley witches’ broom (PrWB). The main objective of (PCR) assays. the present work was to identify and characterize the For long-term maintenance, the PrWB agent was phytoplasmas detected in the symptomatic parsley then graft transmitted to periwinkle plants. Small plants. axillary shoots from a newly infected dodder inocu- lated periwinkle plant from each area were used as scions and side grafted on five 12-week-old seed- Materials and Methods grown periwinkle plants. Each donor plant received two scions. Grafted areas were wrapped with parafilm Source of the disease and plants covered with plastic bags for a week to Sampling of symptomatic parsley was carried out in maintain humidity. Abarkooh, Khatam, Herat-Marvast, Mehreez and Yazd areas, Iran. From each area, two parsley fields DNA extraction and polymerase chain reaction assays were surveyed and two parsley plants (one plant per field) with typical symptoms of witches’ broom Total DNA was extracted from 0.2 g midrib tissue of (PrWB) were selected, potted and transferred to a 10 naturally witches’ broom affected parsley plants greenhouse. They were biweekly sprayed with Meta- from Abarkooh, Khatam, Herat-Marvast, Mehreez systox-R insecticide and used as the sources of the and Yazd areas (two symptomatic plants per area) and PrWB disease in biological and molecular studies. graft and dodder inoculated periwinkle plants (Zhang Twelve-week-old seed-grown periwinkle [Catharan- et al. 1998). Total DNA was extracted from symptom- thus roseus (L.) G. Don] plants were used in dodder less parsley and periwinkle plants and a symptomatic and graft transmission for in vivo maintenance of the periwinkle plant infected with Fars alfalfa witches’ PrWB agent. broom phytoplasma (Salehi et al. 2005), a 16SrII-C strain [(Acc. No. DQ233) (Salehi et al. 2014)]; they were used as negative and positive controls, respec- Disease incidence tively. DNA samples were tested for phytoplasma In each area, four fields were selected randomly and presence using universal phytoplasma primer pair P1/ sampling was carried out at five points in 1000 m2 P7 (Deng and Hiruki 1991; Schneider et al. 1995) in fields within a 1 m2 on a diagonal transect across each direct PCR and followed by R16F2n/R16R2 (Gunder- of the five fields. The percentage of PrWB disease inci- sen and Lee 1996) primer pairs in nested PCR assays. dence was calculated by number of plants with symp- Polymerase chain reaction conditions and reagents toms out of total number of plants observed using the were as reported (Salehi et al. 2014). Polymerase formula given below. chain reaction products were separated in 1% agarose Per cent disease incidence gels in 1X TBE buffer [108 g Tris-HCl, 55 g boric acid, 40 ml EDTA (0.5 M), pH 8.0]. DNA bands were No. of plants infected ¼ Â 100 stained with ethidium bromide and visualized with a Total no. of plants observed UV transilluminator. The molecular weight of the PCR products was estimated by comparison with 100- Dodder and graft transmission bp DNA ladder (Fermentas, Vilnius, Lithuania). Dodder (Cuscuta campestris Yunk.) was used for trans- mission of the PrWB agent from 10 symptomatic pars- Restriction fragment length polymorphism (RFLP) ley plants from Abarkooh, Khatam, Herat-Marvast, analysis Mehreez and Yazd areas (two symptomatic plant per area) to periwinkle plants. For this purpose, seeds of Restriction fragment length polymorphism analysis of dodder were collected in a phytoplasma-free sugar nested PCR products was used for preliminary identi- beet field, germinated on moist filter paper, and seed- fication of the phytoplasma associated with PrWB dis- lings were transferred to 10 witches’ broom affected ease. The R16F2n/R16R2 amplicons were digested parsley plants (each plant in one pot) maintained separately with AluI, HaeIII, HhaI, HpaII, MseI, RsaI under an insect-proof greenhouse. Four weeks later, and TaqI restriction enzymes according to the instruc- each dodder-infested plant was placed adjacent to tions of the manufacturer (Fermentas) at 37°C (65°C three healthy seed-grown periwinkle plants. After for TaqI) overnight. The restriction products were 5 weeks, periwinkle plants were freed of dodder and then separated by a 6.7% polyacrylamide gel elec- kept in the insect-free greenhouse for the observation trophoresis and stained with ethidium bromide. DNA 2 Ó 2016 Blackwell Verlag GmbH M. Salehi et al. Parsley witches’ broom phytoplasma bands were visualized with a UV transilluminator. MEGA6 (Tamura et al. 2013). Acholeplasma laidlawii The resulting RFLP patterns were compared with was used as an out-group to root the trees. Bootstrap- those previously published for 16S rDNA from other ping was performed 1000 times to estimate the stabil- phytoplasma strains (Lee et al. 1998). ity and support for the branches. The 16S rDNA sequence identity between strains was evaluated after alignments generated using homology matrix distance Cloning and sequencing of PCR products option of DNAMAN version 4.02 (Lynnon Biosoft, Que- Ten P1/P7 primed PCR products from the 10 bec, Canada). witches’ broom affected parsley plant samples col- lected from five fields (one field per area and two Virtual RFLP analysis samples per field) in the surveyed areas were ligated onto pTZ57R/T vector and cloned into Virtual RFLP analysis using the iPhyClassifier tool Escherichia coli DH5a cells using InsT/A cloneTM (Zhao et al. 2009) was
Recommended publications
  • Tehran-Textnw29-10A:Mise En Page 1.Qxd
    The designations employed and the presentation of material throughout the publication do not imply the expression of any opinion whatsoever on the part of UNESCO concerning the legal status of any country, territory, city or of its authorities, or concerning the delimitation of its frontiers or boundaries. Published in 2007 by the United Nations Educational, Scientific and Cultural Organization 7, Place de Fontenoy, 75352 Paris 07 SP (France) Composed by Marina Rubio, 93200 Saint-Denis IHP/2007/GW-15 © UNESCO 2007 FOREWORD During the 15th session of the Intergovernmental Council of the International Hydrological Pro- gramme (IHP) the project ‘Groundwater for Emergency Situations (GWES) was approved and included in the Implementation Plan of the Sixth Phase of the IHP (2002–2007) under the title ‘Identification and management of strategic groundwater bodies to be used for emergency situ - ations as a result of extreme events or in case of conflicts’. The aim of the GWES project is 1/ to consider extreme events (natural and man-induced) that could adversely influence human health and life, 2/ to support countries repeatedly affected by such events in the setting up of emergency plans and mitigation schemes to secure drinking water supply, and 3/ to identify in advance potential safe groundwater resources which could temporarily replace damaged water supply systems. The results of this project will allow countries to minimize the dependence of threatened population on vulnerable drinking water supplies. Groundwater bodies are naturally less vulnerable and more resistant than surface waters to external impact. Deep aquifers naturally protected from the earth surface by geological environ- ment should be therefore, identified and evaluated.
    [Show full text]
  • Closer Collaborations with ICARDA MENARID Project
    News Letter; MENARID Int’l Project National News The 4th Project Board Meeting of the MENARID International Project was Held in Sep. 2013 Members of the Project Board of the MENARID Project in Deputy to the Minister of Agricultural Jihad and Head of its fourth meeting discussed on the quality of MENARID Forests, Rangelands and Watershed Organization (FRWO) project processes and activities, assessed the outputs and and MENARID National Project Director (NPD), FRWO required modifications to continue project measures. Watershed Deputy and Actin NPD of the Project, UNDP The session benefited the presence of KhodaKaramJalali, Deputy Resident Representative in Iran and Head of Autumn 2013 - No. 4 64 News Letter; MENARID Int’l Project Inclusive Growth and Development Unit in UNDP- Iran, 1- Transferring the project activities to provinces level as a Representatives from Minister of Foreign Affairs and key policy, Ministry of Agricultural Jihad, Deputy of Planning of 2- Making practical the annual work plan, Sistan and Baluchistan Governor Office, General Director 3- Monitoring and evaluation of the project implementations of Planning of Yazd Governor Office, General Managers through holding regular participatory monitoring and of Natural Resources and Watershed Organizations in evaluation sessions. Kermanshah and Sistan and Baluchistan, MENARID Jazi provided a positive evaluation of the decrees adopted Provincial Project Manager in Yazd and also representatives by the Project Board since its launch and said that in three of local communities on November 30, 2013. last sessions, 21 decrees were discussed and 16 were The session commenced with ParvizGarshasbi, FRWO completely undertaken, 3 other have been adopted, yet, not realized and actions have not been taken regarding 2 other decrees.
    [Show full text]
  • The Role of Creative Economy in the Realization of a Creative City: a Case Study of the City of Meybod in Yazd Province, Iran
    Geographia Polonica 2018, Volume 91, Issue 3, pp. 335-351 https://doi.org/10.7163/GPol.0124 INSTITUTE OF GEOGRAPHY AND SPATIAL ORGANIZATION POLISH ACADEMY OF SCIENCES www.igipz.pan.pl www.geographiapolonica.pl THE ROLE OF CREATIVE ECONOMY IN THE REALIZATION OF A CREATIVE CITY: A CASE STUDY OF THE CITY OF MEYBOD IN YAZD PROVINCE, IRAN Ali Bagheri Kashkouli1 • Asghar Zarabi1 • Mir Najaf Mousavi2 1Department of Geography and Planning Sciences University of Isfahan 81746-73441, Hezar Jarib str., Isfahan: Iran e‑mails: [email protected][email protected] 2Department of Geography Urmia University 5756151818, 165 Urmia: Iran e-mail: [email protected] Abstract In a society, cities are the centers of human interactions, creativity, knowledge, diversity, culture, commerce and economic creativity. Owing to the importance of innovation, knowledge acquisition, and the increased recognition by the government in Iran, many cities have developed strategies and implemented programs to improve their ‘innovative milieus’ and to attract ‘creative people’ in creative industries in order to aid the restructuring and growth of their economy. This paper is a case study serving as a contribution to the current research in the field of small cities, with a focus on the city of Meybod, Yazd. The research examines the factors affecting the attraction and retention of creative people and creative businesses in Meybod, based on the data collected from the Statistical Center of Iran, the management and planning organization of Yazd province, government reports, and key informant interviews. The findings reveal that the attraction of creative people and creative businesses is a complex process.
    [Show full text]
  • Healthmed 6 9 Web.Pdf
    Volume 6 / Number 9 / 2012 HealthJournal of Society for development in new net environmentMED in B&H EDITORIAL BOARD Sadržaj / Table of Contents Editor-in-chief Mensura Kudumovic Comparison of side effects and marital satisfaction Execute Editor Mostafa Nejati between the women taking Cyclofem and Depo Medroxyprogesteron contraceptive ampoules ............... 2944 Associate Editor Azra Kudumovic Maryam Gholamitabar Tabari, Esmaeilzadeh Sedigheh, Technical Editor Eldin Huremovic Ali Bijani, Leily Moslemi Cover design Almir Rizvanbegovic Depression in children and adolescents: family narratives in brazilian primary attention ........................ 2950 Mirza Basic Modesto Leite Rolim Neto, Alberto Olavo Advincula Reis, Luiz Carlos de Abreu, Jose Cezario de Almeida, Marina Members Lucena de Aguiar Ferreira Paul Andrew Bourne (Jamaica) Xiuxiang Liu (China) Physical injuries of homecare Korean patients with senile dementia ........................................................... 2955 Nicolas Zdanowicz (Belgique) Hyung-Sik Kim, Mi-Hyun Choi, Soon-Cheol Chung, Farah Mustafa (Pakistan) Jeong-Han Yi Yann Meunier (USA) Suresh Vatsyayann (New Zealand) Mean platelet volume is incresed in patients Maizirwan Mel (Malaysia) With chronic Hepatitis C .................................................... 2960 Canan Demir, Mehmet Demir Budimka Novakovic (Serbia) Diaa Eldin Abdel Hameed Mohamad (Egypt) Using transcutaneous electrical nerve stimulation Zmago Turk (Slovenia) on acupuncture points for labor augmentation .............. 2965 Chao Chen (Canada)
    [Show full text]
  • Central Iran اﯾران ﻣرﮐزى
    © Lonely Planet Publications 221 Central Iran ﺍﻳﺮﺍﻥ ﻣﺮﮐﺰی Ever since Cyrus the Great’s dramatic rise from provincial overlord to ruler of the largest empire on earth, central Iran has been something of a showcase for the region’s great- est civilisations. The unrelenting splendour and majesty of Esfahan, the refined elegance of Shiraz and the mud-brick antiquity of Yazd, Abyaneh and Kharanaq are a fascinating contrast, representing the fusion over 2500 years of myriad cultures and starkly differ- ent terrains. Then, of course, there’s that monumental expression of artistic harmony commissioned by Darius I – Persepolis. With so much on offer, it’s no surprise that the towns of Iran’s central provinces are where you’ll probably spend the most time. But it’s not just about ticking off the popular sights, because central Iran has many an unsung gem. Kashan, with its splendid mosques, gardens and magnificently restored traditional houses, is one. If you want to get off the beaten track there’s the desert oasis of Garmeh, the cave village of of Garmeh, caravanserai stops such as Zein-o-din and Toudeshk, or the chance to camp with nomads in the Zagros Mountains. CENTRAL IRAN Central Iran’s people are as diverse as the places they live. In Qom they’re conservative and religious, Shirazis are laid-back and fun-loving, and the Qashqa’i and Bakhtiari nomads live a lifestyle dictated by nature. Often you will experience the region’s cultural richness and physical beauty in combination: sitting in the garden of Hafez’s tomb discussing the ways of the world with a Shirazi medical student, perhaps; watching a nomad woman make yogurt by hand; or drinking tea with a carpet salesman in Esfahan’s Imam Sq.
    [Show full text]
  • Curriculum Vitae Gholamhosein Pourghanbari Marvast Avian
    Curriculum Vitae Gholamhosein Pourghanbari Marvast Avian Medicine Specialist Assistant Professor, Avian Medicine Department, School Of Veterinary Medicine, Ardakan University, Ardakan, Yazd, Iran. Phone number: +98-9177098780 Tel: +98-35-32240926 Fax: +98-35-32226767 Email: [email protected], [email protected] Personal Information: . Gender: Male . Date of birth: 22.5.1983 . Nationality: Iranian Education: 2014- present: Assistant Professor, School Of Veterinary Medicine, Ardakan University, Ardakan, Yazd, Iran. 2009 - 2013: PhD Degree in Avian Medicine, School of Veterinary Medicine, Shiraz University, Shiraz .Iran 2001-2008: DVM Degree at School of Veterinary Medicine, Shiraz University ,Shiraz .Iran 2000-2001: Pre-University, Biological Science at High School AveSina. Mehriz, Yazd. Iran 1997-2000: High School Diploma In Biological Science at High School AveSina. Mehriz,Yazd .Iran. 1 Experimental Experience: Expert at: 1. PCR Test 2. Revers Polymeras Chain Reaction Test (RT.PCR) 3. Real Time Polymeras Chain Reaction Test (RT.PCR) 4. ELISA Test 5.HA Test 6.HI Test 7. Cell culture Test 8. Bactrial culture Test 9. Water Binding Capacity Test (WBC) 10. Extract releas valum Test (ERV) 11. Tyrosin Value Test Familiar with: 1.VN Test (Viral Neutralization) 2. Chicken Egg Inoculation (CAM, CAC, Yolk Sac) Observer in: 1.Western Blot Test 2. Immunohistochemistry Test 3. Electron Microscope (EM) 4. HPLC Executive Experience Research Project Leader: Egg Enrichment in Layers by Aromatic Plants. Collage of Veterinary Medicine, Shiraz University.2010- Present. Cooperator of Research Project: Partridge Egg Incubation, Partridge Raising and Study On Influenza Viruses Pathogenesis in Partridge. Collage of Veterinary Medicine, Shiraz University. Shiraz. Iran. 2010. Research and Didactic Project Leader: Ostrich Chicken Raising.
    [Show full text]
  • Groundwater for Emergency Situations
    The designations employed and the presentation of material throughout the publication do not imply the expression of any opinion whatsoever on the part of UNESCO concerning the legal status of any country, territory, city or of its authorities, or concerning the delimitation of its frontiers or boundaries. Published in 2007 by the United Nations Educational, Scientific and Cultural Organization 7, Place de Fontenoy, 75352 Paris 07 SP (France) Composed by Marina Rubio, 93200 Saint-Denis IHP/2007/GW-15 © UNESCO 2007 FOREWORD During the 15th session of the Intergovernmental Council of the International Hydrological Pro- gramme (IHP) the project ‘Groundwater for Emergency Situations (GWES) was approved and included in the Implementation Plan of the Sixth Phase of the IHP (2002–2007) under the title ‘Identification and management of strategic groundwater bodies to be used for emergency situ - ations as a result of extreme events or in case of conflicts’. The aim of the GWES project is 1/ to consider extreme events (natural and man-induced) that could adversely influence human health and life, 2/ to support countries repeatedly affected by such events in the setting up of emergency plans and mitigation schemes to secure drinking water supply, and 3/ to identify in advance potential safe groundwater resources which could temporarily replace damaged water supply systems. The results of this project will allow countries to minimize the dependence of threatened population on vulnerable drinking water supplies. Groundwater bodies are naturally less vulnerable and more resistant than surface waters to external impact. Deep aquifers naturally protected from the earth surface by geological environ- ment should be therefore, identified and evaluated.
    [Show full text]
  • Study of Genetic Diversity in Pomegranate Germplasm of Yazd Province of Iran
    IRANIAN JOURNAL of GENETICS and PLANT BREEDING, Vol. 6, No. 2, Oct 2017 Study of genetic diversity in pomegranate germplasm of Yazd province of Iran Mehdi Zahravi1*, Mohammad Reza Vazifeshenas2 1Department of Genetics and National Plant Gene Bank of Iran, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO). P. O. Box: 31359-33151, Karaj, Iran. 2Agriculture and Natural Resources Research Center of Yazd, Agricultural Research, Education and Extension Organization (AREEO), Yazd, Iran. *Corresponding author, Email: [email protected]. Tel: +98-26-32701260. Fax: +98-26-32716793. Abstract INTRODUCTION A total of 117 pomegranate genotypes collected Pomegranate (Punica granatum L.) is a member of from different areas of Yazd province of Iran were Punicaceae family and is considered for its edible fruits, studied for genetic variation by evaluating 23 medicinal properties and application in food industry morphological traits according to the international (Martinez-Nicolas et al., 2016). Pomegranate is descriptor. Similar diversity pattern of the originated from Iran and neighboring countries (Stover measured characteristics was observed in three and Mercure, 2007). The importance of this product in types of sweet, sweet-sour and sour varieties. The non-oil exports made it to be considered as an export traits shape of fruit base, suckering tendency, vigor commodity in the agricultural sector (Anonymous, of tree, fruit shape and aril color had the highest 2009). Iran is one of the largest pomegranate producers power of discrimination. The results of evaluation in the world (Sarkhosh et al., 2006). About 5% of the of genetic distances revealed discrepancies in the production of horticultural products and about 15% of pairs of genotypes with similar denominations.
    [Show full text]
  • A Comparative Analysis of Facility Level and Population Distribution in Urban Network of Yazd Province
    digitales archiv ZBW – Leibniz-Informationszentrum Wirtschaft ZBW – Leibniz Information Centre for Economics Rastegari, Mohammad Article A comparative analysis of facility level and population distribution in urban network of Yazd province Provided in Cooperation with: Iran Urban Economics Scientific Association, Tehran This Version is available at: http://hdl.handle.net/11159/482 Kontakt/Contact ZBW – Leibniz-Informationszentrum Wirtschaft/Leibniz Information Centre for Economics Düsternbrooker Weg 120 24105 Kiel (Germany) E-Mail: [email protected] https://www.zbw.eu/econis-archiv/ Standard-Nutzungsbedingungen: Terms of use: Dieses Dokument darf zu eigenen wissenschaftlichen Zwecken This document may be saved and copied for your personal und zum Privatgebrauch gespeichert und kopiert werden. Sie and scholarly purposes. You are not to copy it for public or dürfen dieses Dokument nicht für öffentliche oder kommerzielle commercial purposes, to exhibit the document in public, to Zwecke vervielfältigen, öffentlich ausstellen, aufführen, vertreiben perform, distribute or otherwise use the document in public. If oder anderweitig nutzen. Sofern für das Dokument eine Open- the document is made available under a Creative Commons Content-Lizenz verwendet wurde, so gelten abweichend von diesen Licence you may exercise further usage rights as specified in Nutzungsbedingungen die in der Lizenz gewährten Nutzungsrechte. the licence. Leibniz-Informationszentrum Wirtschaft zbw Leibniz Information Centre for Economics A Comparative Analysis of Facility Level and Population Distribution in Urban Network of Yazd Province Mohammad Rastegari* Regional planning, Islamic Azad University, Central Tehran Branch, Tehran, Iran Mohammad Hossein Saraei Department of Geography and Urban Planning, University of Yazd, Yazd, Iran Accepted:2015/11/14 Received:2015/08/16 Abstract: During last three decades, because of rapid influx of population into the cities, many problems have been emerged for cities.
    [Show full text]
  • Some Epidemiological Aspects of Cutaneous Leishmaniasis in a New Focus, Central Iran
    Hindawi Publishing Corporation Dermatology Research and Practice Volume 2015, Article ID 286408, 5 pages http://dx.doi.org/10.1155/2015/286408 Research Article Some Epidemiological Aspects of Cutaneous Leishmaniasis in a New Focus, Central Iran M. R. Yaghoobi-Ershadi,1 N. Marvi-Moghadam,1 R. Jafari,2 A. A. Akhavan,1 H. Solimani,3 A. R. Zahrai-Ramazani,1 M. H. Arandian,2 and A. R. Dehghan-Dehnavi4 1 Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2Isfahan Research Station, National Institute of Health Research, Isfahan, Iran 3Yazd Health Research Station, National Institute of Health Research, Yazd, Iran 4School of Public Health, Yazd University of Medical Sciences, Yazd, Iran Correspondence should be addressed to N. Marvi-Moghadam; nr [email protected] Received 15 July 2015; Accepted 7 September 2015 Academic Editor: Iris Zalaudek Copyright © 2015 M. R. Yaghoobi-Ershadi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Following the epidemic of cutaneous leishmaniasis in Khatam County, YazdProvince, this study was carried out to determine vector, and animal reservoir host(s) and investigate the human infection during 2005-2006. Four rural districts where the disease had higher prevalence were selected. Sticky paper traps were used to collect sand flies during April to November, biweekly. Meanwhile rodents were captured using Sherman traps from August to November. Households and primary schools were visited and examined for human infection in February 2006.
    [Show full text]
  • Advances in Environmental Biology, 8(16) Special 2014, Pages: 391-401
    Advances in Environmental Biology, 8(16) Special 2014, Pages: 391-401 AENSI Journals Advances in Environmental Biology ISSN-1995-0756 EISSN-1998-1066 Journal home page: http://www.aensiweb.com/AEB/ Strategies to Achieve on Sustainable Development Base on Analysis of Environmental Problems (Case Study: Cities of Yazd Province) 1Mir Najaf Mousavi, 2Alireza Soleimani and 3Ali Bagheri Kashkooli 1Associate Prof of Geography and Urban Planning, Urmia University, Iran. 2Department of Geography, Payame noorUniversity, P.O.Box 19395-3697, Iran. 3Ph. D. Student of Geography and Urban Planning, University of Isfahan, Iran. ARTICLE INFO ABSTRACT Article history: Background: Spread of urbanization lead to increasing the environmental problems for Received 30 August 2014 urban residents that it hasn’t agreement with sustainable development purposes. Received in revised form Objective: The aim of this paper is assessment and survey of environmental problems 17 November 2014 in cities of Yazd province base on urban sustainable development indexes with Accepted 25 November 2014 emphasize on the environmental aspects. Thus, methodology is applicable – Available online 8 December 2014 developmental and statistical population is 24 cities of yazd province. Base on last political division in 2011, yazd province has 11 cities, 24 town, 20 section and 51 rural Keywords: districts. Results: Based on statistical data in 2011, the city of yazd with population Environment, Sustainable 489152 persons has about 7 Equal populations than the second meybod city in the Development, Pollution, Waste province. Survey of environmental problems in yazd province shows that rapid Waters, Cities of Yazd Province developmental in industrial fields raised from economical needs and increasing of population in urban regions.
    [Show full text]
  • Assessment and Classification of Environmental Problems Based on Sustainable Development Indexes (Case Study: Cities of Yazd Province)
    Copyright © 2014 Scienceline Publication Journal of Civil Engineering and Urbanism Volume 4, Issue 2: 110-119 (2014) ISSN-2252-0430 Assessment and Classification of Environmental Problems Based on Sustainable Development Indexes (Case Study: Cities of Yazd Province) Mehdi Salehi1, MirNajaf Mousavi2*, Ali Bagheri Kashkooli3 1 PhD in Geography and Urban Planning,, Department of Geography, Baku University, Azarbaijan 2 Assistant Professor of Geography and Urban Planning, Department of Geography, Urmia University, Urmia, Iran 3 PhD Student in Geography and Urban Planning, Department of Geography, University of Isfahan, Iran *Corresponding author’s E-mail: [email protected] ABSTRACT: Spread of urbanization lead to increasing the environmental problems for urban residents that it hasn’t agreement with sustainable development purposes. The aim of this paper is assessment and survey of environmental problems in cities of Yazd province base on urban sustainable development indexes with emphasize on the environmental aspects. Thus, methodology is applicable –developmental and statistical population is 24 cities of Yazd province. Based on last political division in 2011, Yazd province has 11 cities, 24 town, 20 section and 51 rural districts. Based on statistical data in 2011, the city ORIGINAL ARTICLE of Yazd with population 489152 persons, has about 7 Equal population than the second Meybod city in Accepted 03 Oct. 2013 the province. Survey of environmental problems in Yazd province shows that rapid developmental in Received 15 Jun. 2013 industrial fields raised from economical needs and increasing of population in urban regions. In fact in the Yazd province region, most of industrial installation and plants has been built in city of Yazd and they (plants and industrial installation) are important factor for environmental pollution in Yazd.
    [Show full text]