Catalog # Aliquot Size
P10 34G 20 20 g P10 34G 50 50 g
PCTK2 Protein Full-length recombinant human protein expressed in Sf9 cells
Catalog # P10 34G
Lot # Z1204 -3
Product Description Purity
Recombinant full-length human PCTK2 (CDK17) was expressed by baculovirus in Sf9 insect cells using an N- terminal GST tag. This gene accession number is
NM_002595 . The purity of PCTK2 protein was determined to be >75% by Gene Aliases densitometry. Approx. MW 88 kDa . PCTAIRE2; PCTK2
Formulation
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Storage and Stability
Store product at –70 oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background
PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons (1). The identification of a large family of cdc2-related kinases PCTK2 Protein opens the possibility of combinatorial regulation of the Full-length recombinant human protein expressed in Sf9 cells cell cycle together with the emerging large family of cyclins (2). Catalog # P10-34G Lot # Z1204-3 References Purity >75% Concentration 0.1 µ g/ µl 1. Hirose T. et.al: PCTAIRE 2, a Cdc2-related serine/threonine Stability 1yr At –70 oC from date of shipment kinase, is predominantly expressed in terminally Storage & Shipping Store product at –70 oC. For optimal storage, differentiated neurons. Eur J Biochem. 1997 Oct aliquot target into smaller quantities after 15;249(2):481-8. centrifugation and store at recommended temperature. For most favorable performance, 2. Meyerson, M.et.al: A family of human cdc2-related protein avoid repeated handling and multiple kinases. EMBO J. 11: 2909-2917, 1992. freeze/thaw cycles. Product shipped on dry ice.
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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.