PCTK2 Protein Full-Length Recombinant Human Protein Expressed in Sf9 Cells

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Catalog # Aliquot Size P10-34G-20 20 µg P10-34G-50 50 µg PCTK2 Protein Full-length recombinant human protein expressed in Sf9 cells Catalog # P10-34G Lot # Z1204 -3 Product Description Purity Recombinant full-length human PCTK2 (CDK17) was expressed by baculovirus in Sf9 insect cells using an N- terminal GST tag. This gene accession number is NM_002595 . The purity of PCTK2 protein was determined to be >75% by Gene Aliases densitometry. Approx. MW 88 kDa . PCTAIRE2; PCTK2 Formulation Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. Storage and Stability Store product at –70 oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons (1). The identification of a large family of cdc2-related kinases PCTK2 Protein opens the possibility of combinatorial regulation of the Full-length recombinant human protein expressed in Sf9 cells cell cycle together with the emerging large family of cyclins (2). Catalog # P10-34G Lot # Z1204-3 References Purity >75% Concentration 0.1 µ g/ µl 1. Hirose T. et.al: PCTAIRE 2, a Cdc2-related serine/threonine Stability 1yr At –70 oC from date of shipment kinase, is predominantly expressed in terminally Storage & Shipping Store product at –70 oC. For optimal storage, differentiated neurons. Eur J Biochem. 1997 Oct aliquot target into smaller quantities after 15;249(2):481-8. centrifugation and store at recommended temperature. For most favorable performance, 2. Meyerson, M.et.al: A family of human cdc2-related protein avoid repeated handling and multiple kinases. EMBO J. 11: 2909-2917, 1992. freeze/thaw cycles. Product shipped on dry ice. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .

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Profiling Data
Compound Name DiscoveRx Gene Symbol Entrez Gene Percent Compound Symbol Control Concentration (nM) JNK-IN-8 AAK1 AAK1 69 1000 JNK-IN-8 ABL1(E255K)-phosphorylated ABL1 100 1000 JNK-IN-8 ABL1(F317I)-nonphosphorylated ABL1 87 1000 JNK-IN-8 ABL1(F317I)-phosphorylated ABL1 100 1000 JNK-IN-8 ABL1(F317L)-nonphosphorylated ABL1 65 1000 JNK-IN-8 ABL1(F317L)-phosphorylated ABL1 61 1000 JNK-IN-8 ABL1(H396P)-nonphosphorylated ABL1 42 1000 JNK-IN-8 ABL1(H396P)-phosphorylated ABL1 60 1000 JNK-IN-8 ABL1(M351T)-phosphorylated ABL1 81 1000 JNK-IN-8 ABL1(Q252H)-nonphosphorylated ABL1 100 1000 JNK-IN-8 ABL1(Q252H)-phosphorylated ABL1 56 1000 JNK-IN-8 ABL1(T315I)-nonphosphorylated ABL1 100 1000 JNK-IN-8 ABL1(T315I)-phosphorylated ABL1 92 1000 JNK-IN-8 ABL1(Y253F)-phosphorylated ABL1 71 1000 JNK-IN-8 ABL1-nonphosphorylated ABL1 97 1000 JNK-IN-8 ABL1-phosphorylated ABL1 100 1000 JNK-IN-8 ABL2 ABL2 97 1000 JNK-IN-8 ACVR1 ACVR1 100 1000 JNK-IN-8 ACVR1B ACVR1B 88 1000 JNK-IN-8 ACVR2A ACVR2A 100 1000 JNK-IN-8 ACVR2B ACVR2B 100 1000 JNK-IN-8 ACVRL1 ACVRL1 96 1000 JNK-IN-8 ADCK3 CABC1 100 1000 JNK-IN-8 ADCK4 ADCK4 93 1000 JNK-IN-8 AKT1 AKT1 100 1000 JNK-IN-8 AKT2 AKT2 100 1000 JNK-IN-8 AKT3 AKT3 100 1000 JNK-IN-8 ALK ALK 85 1000 JNK-IN-8 AMPK-alpha1 PRKAA1 100 1000 JNK-IN-8 AMPK-alpha2 PRKAA2 84 1000 JNK-IN-8 ANKK1 ANKK1 75 1000 JNK-IN-8 ARK5 NUAK1 100 1000 JNK-IN-8 ASK1 MAP3K5 100 1000 JNK-IN-8 ASK2 MAP3K6 93 1000 JNK-IN-8 AURKA AURKA 100 1000 JNK-IN-8 AURKA AURKA 84 1000 JNK-IN-8 AURKB AURKB 83 1000 JNK-IN-8 AURKB AURKB 96 1000 JNK-IN-8 AURKC AURKC 95 1000 JNK-IN-8
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Sarcoma Genome Project (Phase I) Genome-Wide Molecular Genetic Analysis of 7 Sarcoma Types
Liposarcoma Genomic Alterations Define New Targets for Therapy Samuel Singer, MD Memorial Sloan-Kettering Cancer Center Sarcoma Disease Management Program Chief Gastric and Mixed Tumor Service Liposarcoma • ~20% of 12,000 new STS cases in US each year, the majority are sporadic • Distinct cytogenetic subgroups comprising 5 subtypes • Simple (translocation-associated) − Myxoid and round cell liposarcoma: >90% t(12;16) • Complex rearrangements (alterations in cell cycle genes and checkpoint defects) – Well-differentiated and Dedifferentiated liposarcoma: ~90% 12q amplification – Pleomorphic liposarcoma • Genetic events associated with liposarcomagenesis remain undiscovered Therapeutic Challenges • More than 60% of patients with newly diagnosed liposarcoma eventually die of disease • Diverse histopathology and biological behavior • WDLS and DDLS are not very responsive to chemotherapy • Pressing need to develop subtype specific molecularly targeted therapeutics for patients with advanced/recurrent disease Histologic distribution of Liposarcoma subtype MSKCC Clinical Sarcoma Database 1772 liposarcoma patients over 29 years (1982–2011) Site‐specific histologic subtype distribution WD Sarcoma Genome Project (Phase I) Genome-wide molecular genetic analysis of 7 sarcoma types (Phase I) DNA copy number + LOH 207 sarcomas and Sequencing 225 matched normals genes and 451 Clinical microRNAs annotation database Transcriptional changes (MSKCC) (protein-coding genes) Functional genetic screen of amplified genes in DDLPS Sarcoma Genome Project (Phase
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Targeting Cyclin-Dependent Kinase 9 and Myeloid Cell Leukaemia 1 in MYC-Driven B-Cell Lymphoma
Targeting cyclin-dependent kinase 9 and myeloid cell leukaemia 1 in MYC-driven B-cell lymphoma Gareth Peter Gregory ORCID ID: 0000-0002-4170-0682 Thesis for Doctor of Philosophy September 2016 Sir Peter MacCallum Department of Oncology The University of Melbourne Doctor of Philosophy Submitted in total fulfilment of the degree of Abstract Aggressive B-cell lymphomas include diffuse large B-cell lymphoma, Burkitt lymphoma and intermediate forms. Despite high response rates to conventional immuno-chemotherapeutic approaches, an unmet need for novel therapeutic by resistance to chemotherapy and radiotherapy. The proto-oncogene MYC is strategies is required in the setting of relapsed and refractory disease, typified frequently dysregulated in the aggressive B-cell lymphomas, however, it has proven an elusive direct therapeutic target. MYC-dysregulated disease maintains a ‘transcriptionally-addicted’ state, whereby perturbation of A significant body of evidence is accumulating to suggest that RNA polymerase II activity may indirectly antagonise MYC activity. Furthermore, very recent studies implicate anti-apoptotic myeloid cell leukaemia 1 (MCL-1) as a critical survival determinant of MYC-driven lymphoma. This thesis utilises pharmacologic and genetic techniques in MYC-driven models of aggressive B-cell lymphoma to demonstrate that cyclin-dependent kinase 9 (CDK9) and MCL-1 are oncogenic dependencies of this subset of disease. The cyclin-dependent kinase inhibitor, dinaciclib, and more selective CDK9 inhibitors downregulation of MCL1 are used
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PCTK2 (CDK17)/Cycliny, Active Recombinant Full-Length Human Proteins Expressed in Sf9 Cells
Catalog # Aliquot Size P10-10G -05 5 µg P10-10G -10 10 µg PCTK2 (CDK17)/CyclinY, Active Recombinant full-length human proteins expressed in Sf9 cells Catalog # P10-10G Lot # Z1168-3 Product Description Specific Activity Recombinant full-length human PCTK2 (CDK17) and Cyclin Y (2-end) were co-expressed by baculovirus in Sf9 140,000 insect cells using N-terminal GST tags. The PCTK2 (CDK17) gene accession number is NM_002595 and Cyclin Y is 105,000 NM_145012. 70,000 Gene Aliases 35,000 Activity (cpm) Activity PCTK2 (CDK17): PCTAIRE2; PCTK2 CyclinY: CCNY; C10orf9; CBCP1; CCNX; CFP1 0 0 40 80 120 160 200 Formulation Protein (ng) Recombinant protein stored in 50mM Tris-HCl, pH 7.5, The specific activity of PCTK2 (CDK17)/Cyclin Y was 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM determined to be 24 nmol/min/mg as per activity assay DTT, 0.1mM PMSF, 25% glycerol. protocol. Storage and Stability Purity Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable The purity of PCTK2 (CDK17) performance, avoid repeated handling and multiple /Cyclin Y was determined to be freeze/thaw cycles. >70% by densitometry, PCTK2 (CDK17) approx. MW 88kDa and Scientific Background Cyclin Y Approx. MW 64kDa. PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons (1). The identification of a large family of cdc2-related kinases opens the possibility of combinatorial regulation of the cell PCTK2 (CDK17)/CyclinY, Active cycle together with the emerging large family of cyclins Recombinant full-length human proteins expressed in Sf9 cells (2).
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PCTK2 (CDK17)/Cycliny, Active Recombinant Full-Length Human Proteins Expressed in Sf9 Cells
Catalog # Aliquot Size P10-10G -05 5 µg P10-10G -10 10 µg PCTK2 (CDK17)/CyclinY, Active Recombinant full-length human proteins expressed in Sf9 cells Catalog # P10-10G Lot # Z1335 -1 Product Description Specific Activity Recombinant full-length human PCTK2 (CDK17) and Cyclin Y (2-end) were co-expressed by baculovirus in Sf9 160,000 insect cells using N-terminal GST tags. The PCTK2 (CDK17) gene accession number is NM_002595 and Cyclin Y is 120,000 NM_145012 . 80,000 Gene Aliases 40,000 (cpm) Activity PCTK2 (CDK17): PCTAIRE2; PCTK2 0 CyclinY: CCNY; C10orf9; CBCP1; CCNX; CFP1 0 120 240 360 480 Protein (ng) Formulation The specific activity of PCTK2 (CDK17)/Cyclin Y was determined Recombinant protein stored in 50mM Tris-HCl, pH 7.5, to be 21 nmol /min/mg as per activity assay protocol. 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM Purity DTT, 0.1mM PMSF, 25% glycerol. Storage and Stability Store product at –70 oC. For optimal storage, aliquot The purity of PCTK2 (CDK17) /Cyclin target into smaller quantities after centrifugation and Y was determined to be >75% by store at recommended temperature. For most favorable densitometry, PCTK2 (CDK17) performance, avoid repeated handling and multiple approx. MW 88kDa and Cyclin freeze/thaw cycles. Y Approx. MW 64kDa . Scientific Background PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons (1). The PCTK2 (CDK17)/CyclinY, Active identification of a large family of cdc2-related kinases Recombinant full-length human proteins expressed in Sf9 cells opens the possibility of combinatorial regulation of the Catalog # cell cycle together with the emerging large family of P10-10G Specific Activity 21 nmol/min/mg cyclins (2).
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Human Kinases Info Page
Human Kinase Open Reading Frame Collecon Description: The Center for Cancer Systems Biology (Dana Farber Cancer Institute)- Broad Institute of Harvard and MIT Human Kinase ORF collection from Addgene consists of 559 distinct human kinases and kinase-related protein ORFs in pDONR-223 Gateway® Entry vectors. All clones are clonal isolates and have been end-read sequenced to confirm identity. Kinase ORFs were assembled from a number of sources; 56% were isolated as single cloned isolates from the ORFeome 5.1 collection (horfdb.dfci.harvard.edu); 31% were cloned from normal human tissue RNA (Ambion) by reverse transcription and subsequent PCR amplification adding Gateway® sequences; 11% were cloned into Entry vectors from templates provided by the Harvard Institute of Proteomics (HIP); 2% additional kinases were cloned into Entry vectors from templates obtained from collaborating laboratories. All ORFs are open (stop codons removed) except for 5 (MST1R, PTK7, JAK3, AXL, TIE1) which are closed (have stop codons). Detailed information can be found at: www.addgene.org/human_kinases Handling and Storage: Store glycerol stocks at -80oC and minimize freeze-thaw cycles. To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette tip, puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar plate. To patch the hole, use sterile tape or a portion of a fresh aluminum seal. Note: These plasmid constructs are being distributed to non-profit institutions for the purpose of basic
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Human CDK17 / PCTK2 Gene Cdna Clone Plasmid
Human CDK17 / PCTK2 Gene cDNA clone plasmid Catalog Number: HG11126-M General Information Plasmid Resuspension protocol Gene : PCTAIRE protein kinase 2 1.Centrifuge at 5,000×g for 5 min. Official Symbol : CDK17 2.Carefully open the tube and add 100 l of sterile water to dissolve the DNA. Synonym : PCTAIRE2 3.Close the tube and incubate for 10 minutes at room temperature. 4.Briefly vortex the tube and then do a quick spin to concentrate × Source : Human the liquid at the bottom. Speed is less than 5000 g. 5.Store the plasmid at -20 ℃. cDNA Size: 1572bp The plasmid is ready for: RefSeq : NM_002595.2 • Restriction enzyme digestion • PCR amplification Plasmid: pMD-PCTK2 • E. coli transformation • DNA sequencing Description E.coli strains for transformation (recommended Lot : Please refer to the label on the tube but not limited) Sequence Description : Most commercially available competent cells are appropriate for the plasmid, e.g. TOP10, DH5α and TOP10F´. Identical with the Gene Bank Ref. ID sequence except for the point mutations 1569 T/C not causing the amino acid variation. Vector : pMD18-T Simple Shipping carrier : Each tube contains approximately 10 μg of lyophilized plasmid. Storage : The lyophilized plasmid can be stored at ambient temperature for three months. Quality control : The plasmid is confirmed by full-length sequencing with primers in the sequencing primer list. Sequencing primer list : M13-47 ： 5’ GCCAGGGTTTTCCCAGTCACGAC 3’ RV-M ： 5’ GAGCGGATAACAATTTCACACAGG 3’ Other M13 primers can also be used as sequencing primers. Manufactured By Sino Biological Inc., FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
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Gene Symbol Accession Alias/Prev Symbol Official Full Name AAK1 NM 014911.2 KIAA1048, Dkfzp686k16132 AP2 Associated Kinase 1
Gene Symbol Accession Alias/Prev Symbol Official Full Name AAK1 NM_014911.2 KIAA1048, DKFZp686K16132 AP2 associated kinase 1 (AAK1) AATK NM_001080395.2 AATYK, AATYK1, KIAA0641, LMR1, LMTK1, p35BP apoptosis-associated tyrosine kinase (AATK) ABL1 NM_007313.2 ABL, JTK7, c-ABL, p150 v-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1) ABL2 NM_007314.3 ABLL, ARG v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) (ABL2) ACVR1 NM_001105.2 ACVRLK2, SKR1, ALK2, ACVR1A activin A receptor ACVR1B NM_004302.3 ACVRLK4, ALK4, SKR2, ActRIB activin A receptor, type IB (ACVR1B) ACVR1C NM_145259.2 ACVRLK7, ALK7 activin A receptor, type IC (ACVR1C) ACVR2A NM_001616.3 ACVR2, ACTRII activin A receptor ACVR2B NM_001106.2 ActR-IIB activin A receptor ACVRL1 NM_000020.1 ACVRLK1, ORW2, HHT2, ALK1, HHT activin A receptor type II-like 1 (ACVRL1) ADCK1 NM_020421.2 FLJ39600 aarF domain containing kinase 1 (ADCK1) ADCK2 NM_052853.3 MGC20727 aarF domain containing kinase 2 (ADCK2) ADCK3 NM_020247.3 CABC1, COQ8, SCAR9 chaperone, ABC1 activity of bc1 complex like (S. pombe) (CABC1) ADCK4 NM_024876.3 aarF domain containing kinase 4 (ADCK4) ADCK5 NM_174922.3 FLJ35454 aarF domain containing kinase 5 (ADCK5) ADRBK1 NM_001619.2 GRK2, BARK1 adrenergic, beta, receptor kinase 1 (ADRBK1) ADRBK2 NM_005160.2 GRK3, BARK2 adrenergic, beta, receptor kinase 2 (ADRBK2) AKT1 NM_001014431.1 RAC, PKB, PRKBA, AKT v-akt murine thymoma viral oncogene homolog 1 (AKT1) AKT2 NM_001626.2 v-akt murine thymoma viral oncogene homolog 2 (AKT2) AKT3 NM_181690.1
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Supplemental Table 3 Two-Class Paired Significance Analysis of Microarrays Comparing Gene Expression Between Paired
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Kinome Expression Profiling to Target New Therapeutic Avenues in Multiple Myeloma
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Dissertation Submitted to the Combined Faculties for the Natural
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