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Supplementary material BMJ Open

Table S1 Extent of reproduction of anatomical features in digital and 3D printed model of the specimens. Digital Digital Anatomic feature print Anatomic feature print model model (for P P A A emissary vein)

Superior A A Lambda A A of the A A P NR skull P P P P

Sagittal suture NR NR

Mastoid notch (for P P P P digastric muscle) Occipital groove (for Incisive P P P P ) (jugular Palatine P P P P foramen in its depth) Intermaxillary suture NR NR NR NR

Zygomatic process P P Parietal bone P P

Zygomatic bone P P Occipital bone P P

Frontal bone P P Hypoglossal P P

Inferior of Sphenoidal bone P P Occipital condyle P P the skull Pterygoid process P P and fossa P P

Hamulus NR A Basilar part P P

Pterygoid fossa P P P P

Lateral plate P P NR NR

Greater wing P P Inferior nuchal line P P

Foramen ovale P P P P

Foramen spinosum LP NR Superior nuchal line P NR External occipital Spine P P P P protuberance P P P P

Li Q-Y, et al. BMJ Open 2020; 10:e034900. doi: 10.1136/bmjopen-2019-034900 Supplementary material BMJ Open

Zygomatic process P P Horizontal plate P P

Articular tubercle P P P NR

Mandibular fossa P P Pyramidal process P P

Styloid process P P NR A

Petrotympanic fissure P P P P

Carotid canal P P Choanae P P (external opening)

Tympanic canaliculus NR A P P

External acoustic P P Ala P P Groove for Mastoid process P P pharyngotympanic P P (auditory, eustachian) tube Stylomastoid NR NR Foramen lacerum P P foramen Petrous part P P

Frontal bone P P Ethmoidal bone P P

Glabella P P Orbital plate P P Supra-orbital notch P P Perpendicular plate P P (foramen) Orbital surface P P P NR

Anterior P P P NR of the P P Vomer P P skull Orbital surface of frontal P P P P bone

Orbital surface of lesser Frontal process P P P P wing of sphenoidal bone

Orbital surface P P P NR

Temporal process P P (foramen) P P

Li Q-Y, et al. BMJ Open 2020; 10:e034900. doi: 10.1136/bmjopen-2019-034900 Supplementary material BMJ Open

Zygomaticofacial Orbital surface of greater A A P P foramen wing of sphenoidal hone

Orbital surface of Maxilla P P P P zygomatic bone Zygomaticofacial Zygomatic process P P A A foramen Infra-orbital foramen P P P NR

Alveolar process P P Infra-orbital groove P P

Anterior nasal spine P P Supra-orbital notch P P Posterior and Anterior Coronal suture A A NR A ethmoidal foramina Orbital plate of Parietal bone P P P P ethmoidal bone P P Lacrimal bone P P

Sphenoidal bone P P Fossa for lacrimal sac P P

Orbital process of Lesser wing P P P P palatine bone Orbital surface of Greater wing P P P P maxilla Temporal bone P P lnfra-orbital foramen P P

Glabella P P A A

Alveolar process P P P P

Temporal process P P Inferior orbital P P

Zygomatic arch P P Infratemporal surface of P P

Temporal fossa P P Alveolar foramina A A Lateral of Temporal bone the skull P P Tuberosity of maxilla P P squamous part Zygomatic process P P P P Lateral plate of pterygoid P P P P process Supramastoid crest P P NR A

External acoustic P P External acoustic meatus P P

Li Q-Y, et al. BMJ Open 2020; 10:e034900. doi: 10.1136/bmjopen-2019-034900 Supplementary material BMJ Open

meatus

Mastoid process P P P P

Lambdoid suture P NR Articular tubercle P P

Occipital bone P P P P Atlas of Human Anatomy, 6th edition (edited by Frank H. Netter, MD) was used as a reference book for anatomical annotations, with all listed anatomical features observed on in original bone specimens. Abbreviations: P: present distinctly, LP: lost partly, NR: not well-resolved.

Li Q-Y, et al. BMJ Open 2020; 10:e034900. doi: 10.1136/bmjopen-2019-034900 Supplementary material BMJ Open

Table S2 Sample points’ deviation distribution and average deviation of 3D comparison between specimens and 3D printed models.

Femur Rib Total Total >=Min =Min =Min =Min

Li Q-Y, et al. BMJ Open 2020; 10:e034900. doi: 10.1136/bmjopen-2019-034900 Supplementary material BMJ Open

Table S3 Sample points’ deviation distribution and average deviation of 3D comparison between digital models and 3D printed models.

Femur Rib Total Total >=Min =Min =Min =Min

Li Q-Y, et al. BMJ Open 2020; 10:e034900. doi: 10.1136/bmjopen-2019-034900