DOCSLIB.ORG

Effect of Selenium on Glucosinolate and Isothiocyanate Concentrations in Arabidopsis Thaliana and Rapid-Cycling Brassica Oleracea

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Effect of Selenium on Glucosinolate and Isothiocyanate Concentrations in Arabidopsis Thaliana and Rapid-Cycling Brassica Oleracea University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Masters Theses Graduate School 12-2009 Effect of Selenium on Glucosinolate and Isothiocyanate Concentrations in Arabidopsis thaliana and Rapid-Cycling Brassica oleracea Thomas Casey Barickman University of Tennessee - Knoxville Follow this and additional works at: https://trace.tennessee.edu/utk_gradthes Part of the Plant Sciences Commons Recommended Citation Barickman, Thomas Casey, "Effect of Selenium on Glucosinolate and Isothiocyanate Concentrations in Arabidopsis thaliana and Rapid-Cycling Brassica oleracea. " Master's Thesis, University of Tennessee, 2009. https://trace.tennessee.edu/utk_gradthes/509 This Thesis is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Masters Theses by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a thesis written by Thomas Casey Barickman entitled "Effect of Selenium on Glucosinolate and Isothiocyanate Concentrations in Arabidopsis thaliana and Rapid-Cycling Brassica oleracea." I have examined the final electronic copy of this thesis for form and content and recommend that it be accepted in partial fulfillment of the equirr ements for the degree of Master of Science, with a major in Plant Sciences. Carl E. Sams, Major Professor We have read this thesis and recommend its acceptance: Dennis Deyton, Dr. Dean A. Kopsell Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official studentecor r ds.) To the Graduate Council: I am submitting herewith a thesis written by Thomas Casey Barickman entitled “Effect of Selenium on Glucosinolate and Isothiocyanate Concentrations in Arabidopsis thaliana and Rapid-Cycling Brassica oleracea.” I have examined the final electronic copy of this thesis for form and content and recommend that it be accepted in partial fulfillment of the requirements for the degree of Master of Science with a major in Plant Sciences Carl E. Sams, Major Professor We have read this thesis and recommend its acceptance: Dennis Deyton Dr. Dean A. Kopsell Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official student records.) Effect of Selenium on Glucosinolate and Isothiocyanate Concentrations in Arabidopsis thaliana and Rapid-Cycling Brassica oleracea A Thesis Presented for the Master of Science Degree The University of Tennessee, Knoxville Thomas Casey Barickman December 2009 Copyright © 2009 by Thomas Casey Barickman All rights reserved. iii Acknowledgements I want to thank everyone in my family especially my wife Sommer and daughter Carly for their love, support, and encouragement. I also want to thank my mentor and friend Dr. Carl Sams for his guidance and support not only in my job but my life. I also want to thank Dr. Dean Kopsell for his support and advice on all subjects of my career and everyone who helped me throughout my thesis. iv Abstract Brassica vegetables play a unique nutritional and sensory role in human diets around the world. Their characteristic flavors come from the break down products of glucosinolate (GS) compounds, a large group of nitrogen (N) and sulfur (S) containing glucosides. Glucosinolates are hydrolyzed by myrosinase to isothiocyanates (ITCs) which are biologically active. Mounting evidence of this process is of scientific interest due to the potential for high consumption of Brassica vegetables containing several GSs and their respective hydrolysis products that are associated with cancer chemoprevention. Glucosinolates are sulfur-rich hydrophilic, nonvolatile plant secondary metabolites; and. over the past few decades, their importance has increased following discoveries of their hydrolysis products, ITCs, as potential anticarcinogens. The importance of selenium (Se) to human health has increased in recent years due its antioxidant potential and cancer suppression properties. Recent studies have demonstrated that certain Se containing compounds like Se-methyl-Se-Cysteine and Se-methionine are effective chemoprotective agents, reducing the incidence of breast, liver, prostate, and colorectal cancers in model systems. Brassicaa species are able to hyperaccumulate selenium at concentrations of up to 10-15 mg Se·g-1 dry weight in their shoots while growing on naturally-occurring soils containing only 0.2-10 mg Se·kg-1. The non-specific integration of Se into the S assimilation pathway enables the plant to metabolize selenoamino acids, selenocysteine and selenomethionine, into proteins. The process is believed to be the major contributor of Se toxicity in plants. The ability of hyperaccumulators to accrue and tolerate high concentrations of Se is thought to be associated with a distinct metabolic capacity that enables the plants to convert these selenoamino acids into non-protein amino acids. v Table of Contents Chapter 1: Introduction................................................................................................................... 1 Introduction............................................................................................................................... 2 Rapid cycling Brassica oleracea:........................................................................ 3 Arabidopsis thaliana: ........................................................................................... 4 Glucosinolate Biosynthesis: ................................................................................ 5 Enzymatic Hydrolysis of Glucosinolates:.......................................................... 6 Brassica Vegetables and Disease Prevention:......................................................................... 7 Sulfur.......................................................................................................................................... 9 Selenium................................................................................................................................... 11 Literature Cited ...................................................................................................................... 13 Chapter 2....................................................................................................................................... 25 Impact of Selenium Fertilization on Glucosinolate Concentration in Arabidopsis thaliana and Rapid Cycling Brassica oleracea ................................................................................................. 25 Abstract.................................................................................................................................... 26 Introduction............................................................................................................................. 27 Methods and Materials........................................................................................................... 30 Results and Discussion............................................................................................................ 32 Literature Cited ...................................................................................................................... 35 Chapter 3....................................................................................................................................... 42 The Effect of Selenium Fertilization on Glucosinolate Conversion to Isothiocyanates in Arabidopsis thaliana and Rapid-Cycling Brassica oleracea........................................................ 42 Abstract.................................................................................................................................... 43 Materials and Methods........................................................................................................... 47 Results and Discussion............................................................................................................ 50 Literature Cited ...................................................................................................................... 60 Appendices.................................................................................................................................... 69 Appendix A.............................................................................................................................. 79 Appendix B .............................................................................................................................. 82 Appendix C.............................................................................................................................. 99 SAS Output Statements.......................................................................................................... 99 Vita ......................................................................................................................................... 105 vi List of Tables TABLE 1. GLUCOSINOLATE (GS) CONCENTRATIONS (MEAN ± SE) IN ARABIDOPSIS THALIANA AND RAPID- CYCLING BRASSICA OLERACEALEAF TISSUE GROWN AT DIFFERENT SELENIUM (SE) CONCENTRATIONS....71 TABLE 2. GLUCOSINOLATE (GS) CONCENTRATIONS (MEAN ± SE) IN RAPID-CYCLING BRASSICA OLERACEA GROWN AT DIFFERENT SELENIUM CONCENTRATIONS ...................................................................................72
Load more

Recommended publications
  • The Effect of Processing on the Glucosinolate Profile of Mustard Seed
    The effect of processing on the glucosinolate profile of mustard seed Katherine Cools, and Terry, L.A.* Plant Science Laboratory, Cranfield University, Bedfordshire, MK43 0AL, UK. * Corresponding author. E-mail address: [email protected] (L.A. Terry) Tel.: +44-7500-766-490 Co-author e-mail address: [email protected] (K.Cools) Abstract Brassica juncea mustard seed are used to make mustard paste or condiment. Mustard seed contains glucosinolates which are converted to isothiocyanates following cell disruption by the enzyme, myrosinase. Isothiocyanates are sulphur-containing compounds which give a pungent flavour to the mustard condiment. Three mustard seed cultivars from two seasons were processed into Dijon- and wholegrain-style mustard and glucosinolates and isothiocyanates analysed. Canadian cv. Centennial tended to contain higher glucosinolates compared with the French cv. AZ147 and Ukrainian cv. Choraiva. Conversion of the mustard seed into a wholegrain condiment had less effect on total isothiocyanates and sinigrin content compared with the Dijon preparation. The Canadian mustard cultivars produced wholegrain-style mustard with higher total isothyocyantes and sinigrin compared with the French and Ukrainian cultivars. In summary, results herein suggest that Canadian mustard seed cvs. Centennial and and Forge, and wholegrain processing may result in a condiment with greater bioactive composition. Keywords: Brassica juncea, condiment, cultivar, isothiocyanate, sinigrin. 1 1. INTRODUCTION Brassica juncea L. (syn. Sinapis juncea L.) is a hydrid between B. rapa and B. nigra giving it the characteristics of rapid growth from B. rapa and the mustard oil of B. nigra. There are two forms of B. juncea; the oilseed type and the vegetable type which is used for its edible leaves, stems and roots (Dixon, 2007).
    [Show full text]
  • Isothiocyanates; Sources, Physiological Functions and Food Applications
    1 Plant Archives Vol. 20, Supplement 2, 2020 pp. 2758-2763 e-ISSN:2581-6063 (online), ISSN:0972-5210 ISOTHIOCYANATES; SOURCES, PHYSIOLOGICAL FUNCTIONS AND FOOD APPLICATIONS Mudasir Yaqoob* 1,2 , Poonam Aggarwal 2, Mukul Kumar 1, Neha Purandare 1 1Department of Food Technology and Nutrition, Lovely professional University Phagwara India 2Department of Food Science &Technology Punjab agriculture University Ludhiana Email: [email protected] Abstract Foods are complex mixture of major and minor nutrients. They also contain some non-nutrient mixtures which exert beneficial effects to the human body known as phytochemicals. Phytochemicals are the chemical compounds that are synthesised by the plant cells to fight any kind of stress conditions. Isothiocyanates (ITCs) are the secondary metabolites produced by the plant from the enzymatic hydrolysis of glucosinolates by myrosinase during the plant tissue injury. They are present in Cruciferous vegetables, such as cabbage, broccoli and kale and are sulphur rich compounds. Isothiocyanates have been found to play an important role for the plant against microbial and pest infections. ITCs have been found to have beneficial activities like antibacterial, antifungal, bioherbicidal, and antioxidant, biopesticidal, anticarcinogenic and antimutagenic. Due to these activities they are having applications in food preservation like fruit juices and as an additive in Mayonnaise. Their recent application has been in the food packing’s and in MAP. The present review gives the insight of these phytochemicals, their physiological functions and food applications Keywords : Isothiocyanates, glucosionolate, sources, biocidal, food applications. Introduction in the vapour phase (Isshiki et al., 1992) and due to this characteristic property, it has been able to be used as food Isothiocyanates are the type of phytochemicals that are preservative in a number of foods stuffs.
    [Show full text]
  • Glucosinolates As Undesirable Substances in Animal Feed1
    The EFSA Journal (2008) 590, 1-76 Glucosinolates as undesirable substances in animal feed1 Scientific Panel on Contaminants in the Food Chain (Question N° EFSA-Q-2003-061) Adopted on 27 November 2007 PANEL MEMBERS Jan Alexander, Guðjón Atli Auðunsson, Diane Benford, Andrew Cockburn, Jean-Pierre Cravedi, Eugenia Dogliotti, Alessandro Di Domenico, Maria Luisa Férnandez-Cruz, Peter Fürst, Johanna Fink-Gremmels, Corrado Lodovico Galli, Philippe Grandjean, Jadwiga Gzyl, Gerhard Heinemeyer, Niklas Johansson, Antonio Mutti, Josef Schlatter, Rolaf van Leeuwen, Carlos Van Peteghem and Philippe Verger. SUMMARY Glucosinolates (alkyl aldoxime-O-sulphate esters with a β-D-thioglucopyranoside group) occur in important oil- and protein-rich agricultural crops, including among others Brassica napus (rapeseed of Canola), B. campestris (turnip rape) and Sinapis alba (white mustard), all belonging to the plant family of Brassicaceae. They are present in all parts of these plants, with the highest concentrations often found in seeds. Several of these Brassica species are important feed ingredients and some species are also commonly used in human nutrition such as cauliflower, cabbages, broccoli and Brussels sprouts. Glucosinolates and their breakdown products determine the typical flavour and (bitter) taste of these vegetables. 1For citation purposes: Opinion of the Scientific Panel on Contaminants in the Food Chain on a request from the European Commission on glucosinolates as undesirable substances in animal feed, The EFSA Journal (2008) 590, 1- 76 © European Food Safety Authority, 2008 Glucosinolates as undesirable substances in animal feed The individual glucosinolates vary in structure and the configuration of their side chain. They are hydrophilic and rather stable and remain in the press cake of oilseeds when these are processed and de-oiled.
    [Show full text]
  • Brassica Nigra) Metabolomics, 15(10): 130
    http://www.diva-portal.org This is the published version of a paper published in Metabolomics. Citation for the original published paper (version of record): Papazian, S., Girdwood, T., Wessels, B A., Poelman, E H., Dicke, M. et al. (2019) Leaf metabolic signatures induced by real and simulated herbivory in black mustard (Brassica nigra) Metabolomics, 15(10): 130 https://doi.org/10.1007/s11306-019-1592-4 Access to the published version may require subscription. N.B. When citing this work, cite the original published paper. Permanent link to this version: http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-164033 Metabolomics (2019) 15:130 https://doi.org/10.1007/s11306-019-1592-4 ORIGINAL ARTICLE Leaf metabolic signatures induced by real and simulated herbivory in black mustard (Brassica nigra) Stefano Papazian1 · Tristan Girdwood1 · Bernard A. Wessels1 · Erik H. Poelman2 · Marcel Dicke2 · Thomas Moritz3 · Benedicte R. Albrectsen1 Received: 30 April 2019 / Accepted: 12 September 2019 © The Author(s) 2019 Abstract Introduction The oxylipin methyl jasmonate (MeJA) is a plant hormone active in response signalling and defence against herbivores. Although MeJA is applied experimentally to mimic herbivory and induce plant defences, its downstream efects on the plant metabolome are largely uncharacterized, especially in the context of primary growth and tissue-specifcity of the response. Objectives We investigated the efects of MeJA-simulated and real caterpillar herbivory on the foliar metabolome of the wild plant Brassica nigra and monitored the herbivore-induced responses in relation to leaf ontogeny. Methods As single or multiple herbivory treatments, MeJA- and mock-sprayed plants were consecutively exposed to cater- pillars or left untreated.
    [Show full text]
  • Brassica Oleracea Var. Capitata) Germplasm
    Article Profiling of Individual Desulfo-Glucosinolate Content in Cabbage Head (Brassica oleracea var. capitata) Germplasm Shiva Ram Bhandari 1, Juhee Rhee 2, Chang Sun Choi 3, Jung Su Jo 1, Yu Kyeong Shin 1 and Jun Gu Lee 1,4,* 1 Department of Horticulture, College of Agriculture & Life Sciences, Jeonbuk National University, Jeonju 54896, Korea; [email protected] (S.R.B.), [email protected] (J.S.J.), [email protected] (Y.K.S.) 2 National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea; [email protected] 3 Breeding Research Institute, Koregon Co., Ltd, Gimje 54324, Korea; [email protected] 4 Institute of Agricultural Science & Technology, Jeonbuk National University, Jeonju 54896, Korea * Correspondence: [email protected]; Tel.: +82-63-270-2578 Received: 24 March 2020; Accepted: 16 April 2020; Published: 17 April 2020 Abstract: Individual glucosinolates (GSLs) were assessed to select cabbage genotypes for a potential breeding program. One hundred forty-six cabbage genotypes from different origins were grown in an open field from March to June 2019; the cabbage heads were used for GSL analyses. Seven aliphatics [glucoiberin (GIB), progoitrin (PRO), epi-progoitrin (EPI), sinigrin (SIN), glucoraphanin (GRA), glucoerucin (GER) and gluconapin (GNA)], one aromatic [gluconasturtiin (GNS)] and four indolyl GSLs [glucobrassicin (GBS), 4-hydroxyglucobrassicin (4HGBS), 4-methoxyglucobrassicin (4MGBS), neoglucobrassicin (NGBS)] were found this study. Significant variation was observed in the individual GSL content and in each class of GSLs among the cabbage genotypes. Aliphatic GSLs were predominant (58.5%) among the total GSLs, followed by indolyl GSL (40.7%) and aromatic GSLs (0.8%), showing 46.4, 51.2 and 137.8% coefficients of variation, respectively.
    [Show full text]
  • Development of an Efficient Glucosinolate Extraction Method
    This is a repository copy of Development of an efficient glucosinolate extraction method. White Rose Research Online URL for this paper: https://eprints.whiterose.ac.uk/113468/ Version: Accepted Version Article: Doheny-Adams, Timothy, Redeker, Kelly Robert orcid.org/0000-0002-1903-2286, Kittipol, Varanya et al. (2 more authors) (2017) Development of an efficient glucosinolate extraction method. Plant Methods. 17. ISSN 1746-4811 https://doi.org/10.1186/s13007-017-0164-8 Reuse This article is distributed under the terms of the Creative Commons Attribution (CC BY) licence. This licence allows you to distribute, remix, tweak, and build upon the work, even commercially, as long as you credit the authors for the original work. More information and the full terms of the licence here: https://creativecommons.org/licenses/ Takedown If you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing [email protected] including the URL of the record and the reason for the withdrawal request. [email protected] https://eprints.whiterose.ac.uk/ Plant M ethods Development of an efficient glucosinolate extraction method --Manuscript Draft-- Manuscript Number: PLME-D-16-00100R1 Full Title: Development of an efficient glucosinolate extraction method Article Type: Methodology Funding Information: Biotechnology and Biological Sciences Prof Ian Bancroft Research Council (BB/L002124/1) Biotechnology and Biological Sciences Prof Sue E Hartley Research Council (BB/K020463/1) Abstract: Abstract Background Glucosinolates, anionic sulfur rich secondary metabolites, have been extensively studied because of their occurrence in the agriculturally important brassicacae and their impact on human and animal health.
    [Show full text]
  • The Role and Effects of Glucosinolates of Brassica Species – a Review
    The role and effects of glucosinolates of Brassica species a review H. Zukalová, J. Vaák Czech University of Agriculture in Prague, Czech Republic ABSTRACT Glucosinolates are the substituted esters of thio amino acids and their synthesis is based on the corresponding amino acids. Methionine and cysteine are the natural donors in the case of the Brassica plants and L-tryptophane in the indole glucosinolates, respectively. In Brassica genus, alkenyl glucosinolates are mostly present and their content and composi- tion differ as far as the development stage and the part of the plant are concerned. The indole glucosinolates are present in a minority level. Their role of sulphur supply is questioned by their very low content between 2% in the beginning of vegetation and 0.1% in its end. Glucosinolates are discussed mostly from the aspect of their anti-nutrition, anti-microbial, anti-fungicidal, and anti-bacterial effects and as being natural bio-fumigants. Their decomposition products have the men- tioned properties. The products originate by prepared passive protection by the two-component system. From the as- pect of these properties, it is useful to divide them into the following three groups according to the characters of their decomposition products. The first group (I.), whose hydrolysis in the neutral and alkaline environment creates iso-thio- cyanates. These bioactive compounds form the natural protection of the plant with bio-fumigatory effects particularly. Their anti-nutritive effects can be compensated by iodine, contrary to the second group (II.). This group is created by hydroxy-glucosinolates, whose decomposition products – iso-thio-cyanates – are not stable and they cycle while produc- ing substituted 2-oxazolidinethione (goitrine – VTO).
    [Show full text]
  • Sinalbin Degradation Products in Mild Yellow Mustard Paste
    Sinalbin degradation products in mild yellow mustard paste Dragana Paunović1, Tatjana Šolević Knudsen2, Mirjana Krivokapić1, Branislav Zlatković1, Mališa Antić1,2 1University of Belgrade, Faculty of Agriculture, Belgrade, Serbia 2University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Belgrade, Serbia Abstract Sinalbin degradation products in mild yellow mustard paste were investigated. The anal- PROFESSIONAL PAPER yzed material consisted of a mild yellow mustard paste condiment and ground white mus- tard seeds which were originally used in the mustard paste production process. The samp- UDC 664.53:543.544.3.547.918 les were extracted in a Soxhlet extraction system and analyzed by gas chromatography– –mass spectrometry (GC–MS) technique. The only sinalbin degradation product in ground mustard seeds was 2-(4-hydroxyphenyl)acetonitrile. The most abundant sinalbin degrada- Hem. Ind. 66 (1) 29–32 (2012) tion product in yellow mustard paste was 4-(hydroxymethyl)phenol. Other compounds identified in this sample were: 4-methyl phenol, 4-ethyl phenol, 4-(2-hydroxyethyl)phenol doi: 10.2298/HEMIND110627055P and 2-(4-hydroxyphenyl) ethanoic acid. Keywords: mild mustard paste; sinalbin degradation products; extraction; GC–MS. Available online at the Journal website: http://www.ache.org.rs/HI/ Mustard is a condiment made from whole, pow- Although the chemical composition of mustard dered or crushed dried seeds of mustard plants: Bras- seeds, as well as degradation pathways of related glu- sica nigra (black mustard), Brassica juncea (brown mus- cosinolates, have been investigated in detail, only a few tard) or Sinapis alba (white mustard). The seeds are papers on the chemical composition of mustard paste usually mixed with wine, vinegar, water and various created from brown and black mustard have been pub- spices to create a paste ranging in color from bright yel- lished so far [9,10], and to the best of our knowledge, low to dark brown.
    [Show full text]
  • Thioglycosides and Cancer Prevention
    Archives of Organic and Inorganic L UPINE PUBLISHERS Chemical Sciences Open Access DOI: 10.32474/AOICS.2018.02.000141 ISSN: 2637-4609 Review Article Thioglycosides and Cancer Prevention Anubhuti Sharma*, Ashok Kumar, Arun Kumar, HS Meena, Prashant Yadav, Priti Gupta, Vijaya Laxmi Tripathi, Aadityendra K Sharma and PK Rai ICAR-DRMR, India Received: March 26, 2018; Published: April 06, 2018 *Corresponding author: Anubhuti Sharma, ICAR-DRMR, Bharatpur, Rajasthan, India, Tel: ; Email: Abstract Plant food contains numerous nutritional & anti-nutritional compounds, which prevents various non-communicable diseases such as cancer. This paper gives an overview of the cancer preventive effect of brassica. In recent years, considerable attention has been paid to cancer protective role of Brassica through major secondary metabolites i.e. glucosinolates. Glucosinolates and their breakdown products have been widely studied for their biological effect on human and animal nutrition. These are responsible for the secretion of detoxifying enzymes that remove carcinogens from the organism. Furthermore, they activate proteins and II phase detoxifying enzymes. They act as a major defence system to plants and humans. However breakdown products of glucosinolates e.g. isothiocyanate and indole regulates cancer cell development by regulating target enzymes, which are involved in controlling apoptosis and blocking of the cell cycle. The present paper reviews the available literature on glucosinolates, their biosynthesis, Keywords:hydrolysis, their Glucosinolates; health promoting Brassica; beneficial Isothiocyanates; effects and Phase possible II enzymes role in imparting defence against diseases especially cancer. Introduction selenium, organo sulphur compounds and ascorbic acids etc. Rapeseed-mustard group of crops is considered as the oldest [2] with anti-proliferative activities.
    [Show full text]
  • Development of HPLC Method for Quantification of Sinigrin
    molecules Article Development of HPLC Method for Quantification of Sinigrin from Raphanus sativus Roots and Evaluation of Its Anticancer Potential Anroop B. Nair 1,* , Dipal Gandhi 2 , Snehal S. Patel 3 , Mohamed A. Morsy 1,4 , Bapi Gorain 5,6, Mahesh Attimarad 1 and Jigar N. Shah 7 1 Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa 31982, Saudi Arabia; [email protected] (M.A.M.); [email protected] (M.A.) 2 Department of Pharmacognosy, Institute of Pharmacy, Nirma University, Ahmedabad 382481, Gujarat, India; [email protected] 3 Department of Pharmacology, Institute of Pharmacy, Nirma University, Ahmedabad 382481, Gujarat, India; [email protected] 4 Department of Pharmacology, Faculty of Medicine, Minia University, El-Minia 61511, Egypt 5 School of Pharmacy, Faculty of Health and Medical Sciences, Taylor’s University, Subang Jaya, Selangor 47500, Malaysia; [email protected] 6 Centre for Drug Delivery and Molecular Pharmacology, Faculty of Health and Medical Sciences, Taylor’s University, Subang Jaya, Selangor 47500, Malaysia 7 Department of Pharmaceutics, Institute of Pharmacy, Nirma University, Ahmedabad 382481, Gujarat, India; [email protected] * Correspondence: [email protected]; Tel.: +966-536-219-868 Academic Editor: Natalizia Miceli Received: 14 October 2020; Accepted: 23 October 2020; Published: 26 October 2020 Abstract: Sinigrin, a precursor of allyl isothiocyanate, present in the Raphanus sativus exhibits diverse biological activities, and has an immense role against cancer proliferation. Therefore, the objective of this study was to quantify the sinigrin in the R. sativus roots using developed and validated RP-HPLC method and further evaluated its’ anticancer activity.
    [Show full text]
  • A Straightforward Method for Glucosinolate Extraction and Analysis with High-Pressure Liquid Chromatography (HPLC)
    Journal of Visualized Experiments www.jove.com Video Article A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC) Katharina Grosser1,2, Nicole M. van Dam1,2 1 German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig 2 Institute of Ecology, Friedrich Schiller University Jena Correspondence to: Nicole M. van Dam at [email protected] URL: https://www.jove.com/video/55425 DOI: doi:10.3791/55425 Keywords: Chemistry, Issue 121, Arabidopsis, Brassica, cabbage, European community official method, mustard, high-pressure liquid chromatography-photodiode array (HPLC-PDA), indole glucosinolates, oilseed rape, response factors Date Published: 3/15/2017 Citation: Grosser, K., van Dam, N.M. A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC). J. Vis. Exp. (121), e55425, doi:10.3791/55425 (2017). Abstract Glucosinolates are a well-studied and highly diverse class of natural plant compounds. They play important roles in plant resistance, rapeseed oil quality, food flavoring, and human health. The biological activity of glucosinolates is released upon tissue damage, when they are mixed with the enzyme myrosinase. This results in the formation of pungent and toxic breakdown products, such as isothiocyanates and nitriles. Currently, more than 130 structurally different glucosinolates have been identified. The chemical structure of the glucosinolate is an important determinant of the product that is formed, which in turn determines its biological activity. The latter may range from detrimental (e.g., progoitrin) to beneficial (e.g., glucoraphanin). Each glucosinolate-containing plant species has its own specific glucosinolate profile. For this reason, it is important to correctly identify and reliably quantify the different glucosinolates present in brassicaceous leaf, seed, and root crops or, for ecological studies, in their wild relatives.
    [Show full text]
  • Bioavailability of Glucosinolates and Their Breakdown Products
    Bioavailability of glucosinolates and their breakdown products impact of processing Barba Orellana, Francisco Jose; Nikmaram, Nooshin; Roohinejad, Shahin; Khelfa, Anissa; Zhu, Zhenzhou; Koubaa, Mohamed Published in: Frontiers in Nutrition DOI: 10.3389/fnut.2016.00024 Publication date: 2016 Document version Publisher's PDF, also known as Version of record Citation for published version (APA): Barba Orellana, F. J., Nikmaram, N., Roohinejad, S., Khelfa, A., Zhu, Z., & Koubaa, M. (2016). Bioavailability of glucosinolates and their breakdown products: impact of processing. Frontiers in Nutrition, 3, [24]. https://doi.org/10.3389/fnut.2016.00024 Download date: 30. sep.. 2021 REVIEW published: 16 August 2016 doi: 10.3389/fnut.2016.00024 Bioavailability of Glucosinolates and Their Breakdown Products: Impact of Processing Francisco J. Barba1,2*, Nooshin Nikmaram3, Shahin Roohinejad4, Anissa Khelfa5, Zhenzhou Zhu6 and Mohamed Koubaa5 1 Department of Food Science, Faculty of Science, University of Copenhagen, Copenhagen, Denmark, 2 Nutrition and Food Science Area, Faculty of Pharmacy, Universitat de València, València, Spain, 3 Department of Food Science and Technology, Faculty of Agricultural Engineering, Islamic Azad University of Sabzevar, Sabzevar, Iran, 4Burn and Wound Healing Research Center, Division of Food and Nutrition, Shiraz University of Medical Sciences, Shiraz, Iran, 5 Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Centre de Recherche de Royallieu, Compiègne Cedex, France, 6 School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China Glucosinolates are a large group of plant secondary metabolites with nutritional effects, and are mainly found in cruciferous plants. After ingestion, glucosinolates could be partially absorbed in their intact form through the gastrointestinal mucosa.
    [Show full text]