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JOURNAL OF VIROLOGY Volume 80 September 2006 No. 18 SPOTLIGHT Articles of Significant Interest Selected from This Issue by 8847 the Editors STRUCTURE AND ASSEMBLY Alphavirus Capsid Protein Helix I Controls a Checkpoint Eunmee M. Hong, Rushika Perera, 8848–8855 in Nucleocapsid Core Assembly and Richard J. Kuhn Mutation at Residue 523 Creates a Second Receptor Tatiana Bousse and Toru Takimoto 9009–9016 Binding Site on Human Parainfluenza Virus Type 1 Hemagglutinin-Neuraminidase Protein Proteomic and Biochemical Analysis of Purified Human Elena Chertova, Oleg Chertov, Lori 9039–9052 Immunodeficiency Virus Type 1 Produced from Infected V. Coren, James D. Roser, Charles Monocyte-Derived Macrophages M. Trubey, Julian W. Bess, Jr., Raymond C. Sowder II, Eugene Barsov, Brian L. Hood, Robert J. Fisher, Kunio Nagashima, Thomas P. Conrads, Timothy D. Veenstra, Jeffrey D. Lifson, and David E. Ott Oligomerization of Hantavirus Nucleocapsid Protein: Agne Alminaite, Vera Halttunen, 9073–9081 Analysis of the N-Terminal Coiled-Coil Domain Vibhor Kumar, Antti Vaheri, Liisa Holm, and Alexander Plyusnin Crystal Structure of the Receptor-Binding Protein Head Stefano Ricagno, Vale´rie 9331–9335 Domain from Lactococcus lactis Phage bIL170 Campanacci, Ste´phanie Blangy, Silvia Spinelli, Denise Tremblay, Sylvain Moineau, Mariella Tegoni, and Christian Cambillau GENOME REPLICATION AND REGULATION OF VIRAL GENE EXPRESSION High-Throughput, Library-Based Selection of a Murine Julie H. Yu and David V. Schaffer 8981–8988 Leukemia Virus Variant To Infect Nondividing Cells Temporal Transcription Program of Recombinant Shih Sheng Jiang, I-Shou Chang, 8989–8999 Autographa californica Multiple Nucleopolyhedrosis Virus Lin-Wei Huang, Po-Cheng Chen, Chi-Chung Wen, Shu-Chen Liu, Li- Chu Chien, Chung-Yen Lin, Chao A. Hsiung, and Jyh-Lyh Juang Proteomic Analysis of the Kaposi’s Sarcoma-Associated Huaxin Si, Subhash C. Verma, and 9017–9030 Herpesvirus Terminal Repeat Element Binding Proteins Erle S. Robertson Rotavirus Nonstructural Protein NSP3 Is Not Required Hilda Montero, Carlos F. Arias, and 9031–9038 for Viral Protein Synthesis Susana Lopez Gene-Specific Inhibition of Reovirus Replication by RNA Takeshi Kobayashi, James D. 9053–9063 Interference Chappell, Pranav Danthi, and Terence S. Dermody Amino Acids in the Basic Domain of Epstein-Barr Virus Lee Heston, Ayman El-Guindy, Jill 9115–9133 ZEBRA Protein Play Distinct Roles in DNA Binding, Countryman, Charles Dela Cruz, Activation of Early Lytic Gene Expression, and Promotion Henri-Jacques Delecluse, and of Viral DNA Replication George Miller A Pseudoknot in a Preactive Form of a Viral RNA Is Part Jiuchun Zhang, Guohua Zhang, 9181–9191 of a Structural Switch Activating Minus-Strand Synthesis Rong Guo, Bruce A. Shapiro, and Anne E. Simon Continued on following page Continued from preceding page Cross-Interaction between JC Virus Agnoprotein and Dorota Kaniowska, Rafal Kaminski, 9288–9299 Human Immunodeficiency Virus Type 1 (HIV-1) Tat Shohreh Amini, Sujatha Modulates Transcription of the HIV-1 Long Terminal Radhakrishnan, Jay Rappaport, Repeat in Glial Cells Edward Johnson, Kamel Khalili, Luis Del Valle, and Armine Darbinyan An Element of the Tertiary Structure of Peach Latent Carmen Herna´ndez, Francesco Di 9336–9340 Mosaic Viroid RNA Revealed by UV Irradiation Serio, Silvia Ambro´s, Jose´-Antonio Daro`s, and Ricardo Flores GENETIC DIVERSITY AND EVOLUTION Phylogenetic Analysis of Polyomavirus BK Sequences Preety M. Sharma, Gaurav Gupta, 8869–8879 Abhay Vats, Ron Shapiro, and Parmjeet Randhawa Genome of Horsepox Virus E. R. Tulman, G. Delhon, C. L. 9244–9258 Afonso, Z. Lu, L. Zsak, N. T. Sandybaev, U. Z. Kerembekova, V. L. Zaitsev, G. F. Kutish, and D. L. Rock Genome Characterization of Lipid-Containing Marine Mart Krupovicˇ, Heikki Vilen, Jaana 9270–9278 Bacteriophage PM2 by Transposon Insertion Mutagenesis K. H. Bamford, Hanna M. Kivela¨, Juha-Matti Aalto, Harri Savilahti, and Dennis H. Bamford Complete Genome Sequence of the Broad Host Range Tanja M. Ruokoranta, A. Marika 9326–9330 Single-Stranded RNA Phage PRR1 Places It in the Levivirus Grahn, Janne J. Ravantti, Minna M. Genus with Characteristics Shared with Alloleviviruses Poranen, and Dennis H. Bamford VIRUS-CELL INTERACTIONS Identification of Amino Acid Residues within Simian Virus Akira Nakanishi, Akiko Nakamura, 8891–8898 40 Capsid Proteins Vp1, Vp2, and Vp3 That Are Required Robert Liddington, and Harumi for Their Interaction and for Viral Infection Kasamatsu Vaccinia Virus Entry into Cells via a Low-pH-Dependent Alan C. Townsley, Andrea S. 8899–8908 Endosomal Pathway Weisberg, Timothy R. Wagenaar, and Bernard Moss Kaposi’s Sarcoma-Associated Herpesvirus Latency- Jianxin You, Viswanathan 8909–8919 Associated Nuclear Antigen Interacts with Bromodomain Srinivasan, Gerald V. Denis, Protein Brd4 on Host Mitotic Chromosomes William J. Harrington, Jr., Mary E. Ballestas, Kenneth M. Kaye, and Peter M. Howley Human Cytomegalovirus Inhibits Neuronal Differentiation Jenny Odeberg, Nina Wolmer, Scott 8929–8939 and Induces Apoptosis in Human Neural Precursor Cells Falci, Magnus Westgren, Åke Seiger, and Cecilia So¨derberg- Naucle´r Keratinocyte-Secreted Laminin 5 Can Function as a Timothy D. Culp, Lynn R. Budgeon, 8940–8950 Transient Receptor for Human Papillomaviruses by Binding M. Peter Marinkovich, Guerrino Virions and Transferring Them to Adjacent Cells Meneguzzi, and Neil D. Christensen Adeno-Associated Virus Type 2 Contains an Integrin ␣5␤1 Aravind Asokan, Julie B. Hamra, 8961–8969 Binding Domain Essential for Viral Cell Entry Lakshmanan Govindasamy, Mavis Agbandje-McKenna, and Richard J. Samulski Role for 3-O-Sulfated Heparan Sulfate as the Receptor for Vaibhav Tiwari, Christian Clement, 8970–8980 Herpes Simplex Virus Type 1 Entry into Primary Human Ding Xu, Tibor Valyi-Nagy, Corneal Fibroblasts Beatrice Y. J. T. Yue, Jian Liu, and Deepak Shukla Translocation of Tomato Bushy Stunt Virus P19 Protein into Tomas Canto, Joachim F. Uhrig, 9064–9072 the Nucleus by ALY Proteins Compromises Its Silencing Maud Swanson, Kathryn M. Wright, Suppressor Activity and Stuart A. MacFarlane Continued on following page Continued from preceding page Transcriptional Profiling Reveals a Possible Role for the Thomas Ruby, Catherine Whittaker, 9207–9216 Timing of the Inflammatory Response in Determining David R. Withers, Mounira K. Susceptibility to a Viral Infection Chelbi-Alix, Veronique Morin, Anne Oudin, John R. Young, and Rima Zoorob Modulation of the Unfolded Protein Response by the Severe Ching-Ping Chan, Kam-Leung Siu, 9279–9287 Acute Respiratory Syndrome Coronavirus Spike Protein King-Tung Chin, Kwok-Yung Yuen, Bojian Zheng, and Dong-Yan Jin Herpes Simplex Virus Type 1 Latently Infected Neurons Se´verine Maillet, Thierry Naas, 9310–9321 Differentially Express Latency-Associated and ICP0 Sophie Crepin, Anne-Marie Roque- Transcripts Afonso, Florence Lafay, Stacey Efstathiou, and Marc Labetoulle The Virion Host Shutoff Protein (UL41) of Herpes Simplex Brunella Taddeo and Bernard 9341–9345 Virus 1 Is an Endoribonuclease with a Substrate Specificity Roizman Similar to That of RNase A CELLULAR RESPONSE TO INFECTION CD80 and CD86 Control Antiviral CD8؉ T-Cell Function Shinichiro Fuse, Joshua J. Obar, 9159–9170 and Immune Surveillance of Murine Gammaherpesvirus 68 Sarah Bellfy, Erica K. Leung, Weijun Zhang, and Edward J. Usherwood Poxvirus Tumor Necrosis Factor Receptor (TNFR)-Like T2 Lisa M. Sedger, Sarah R. Osvath, 9300–9309 Proteins Contain a Conserved Preligand Assembly Domain Xiao-Ming Xu, Grace Li, Francis That Inhibits Cellular TNFR1-Induced Cell Death K.-M. Chan, John W. Barrett, and Grant McFadden TRANSFORMATION AND ONCOGENESIS An Immunoreceptor Tyrosine Activation Motif in the Mouse Susan R. Ross, John W. Schmidt, 9000–9008 Mammary Tumor Virus Envelope Protein Plays a Role in Elad Katz, Laura Cappelli, Stacy Virus-Induced Mammary Tumors Hultine, Phyllis Gimotty, and John G. Monroe Envelope Proteins of Jaagsiekte Sheep Retrovirus and Sarah K. Wootton, Christine L. 9322–9325 Enzootic Nasal Tumor Virus Induce Similar Halbert, and A. Dusty Miller Bronchioalveolar Tumors in Lungs of Mice GENE DELIVERY ␣2,3 and ␣2,6 N-Linked Sialic Acids Facilitate Efficient Zhijian Wu, Edward Miller, Mavis 9093–9103 Binding and Transduction by Adeno-Associated Virus Types Agbandje-McKenna, and Richard 1 and 6 Jude Samulski VACCINES AND ANTIVIRAL AGENTS AIDS Vaccination Studies with an Ex Vivo Feline Mauro Pistello, Francesca Bonci, 8856–8868 Immunodeficiency Virus Model: Analysis of the Accessory J. Norman Flynn, Paola Mazzetti, ORF-A Protein and DNA as Protective Immunogens Patrizia Isola, Elisa Zabogli, Valentina Camerini, Donatella Matteucci, Giulia Freer, Paolo Pelosi, and Mauro Bendinelli Vaginal Protection and Immunity after Oral Immunization Xinyan Zhao, Manxin Zhang, 8880–8890 of Mice with a Novel Vaccine Strain of Listeria Zhongxia Li, and Fred R. Frankel monocytogenes Expressing Human Immunodeficiency Virus Type 1 gag Baculovirus-Derived Human Immunodeficiency Virus Type 1 L. Buonaguro, M. L. Tornesello, 9134–9143 Virus-Like Particles Activate Dendritic Cells and Induce Ex M. Tagliamonte, R. C. Gallo, L. X. Vivo T-Cell Responses Wang, R. Kamin-Lewis, S. Abdelwahab, G. K. Lewis, and F. M. Buonaguro Continued on following page Continued from preceding page Histatin 5-Derived Peptide with Improved Fungicidal Fedde Groot, Rogier W. Sanders, 9236–9243 Properties Enhances Human Immunodeficiency Virus Olivier ter Brake, Kamran Nazmi, Type 1 Replication by Promoting Viral Entry Enno C. I. Veerman,
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  • APICAL M2 PROTEIN IS REQUIRED for EFFICIENT INFLUENZA a VIRUS REPLICATION by Nicholas Wohlgemuth a Dissertation Submitted To

    APICAL M2 PROTEIN IS REQUIRED for EFFICIENT INFLUENZA a VIRUS REPLICATION by Nicholas Wohlgemuth a Dissertation Submitted To

    APICAL M2 PROTEIN IS REQUIRED FOR EFFICIENT INFLUENZA A VIRUS REPLICATION by Nicholas Wohlgemuth A dissertation submitted to Johns Hopkins University in conformity with the requirements for the degree of Doctor of Philosophy Baltimore, Maryland October, 2017 © Nicholas Wohlgemuth 2017 All rights reserved ABSTRACT Influenza virus infections are a major public health burden around the world. This dissertation examines the influenza A virus M2 protein and how it can contribute to a better understanding of influenza virus biology and improve vaccination strategies. M2 is a member of the viroporin class of virus proteins characterized by their predicted ion channel activity. While traditionally studied only for their ion channel activities, viroporins frequently contain long cytoplasmic tails that play important roles in virus replication and disruption of cellular function. The currently licensed live, attenuated influenza vaccine (LAIV) contains a mutation in the M segment coding sequence of the backbone virus which confers a missense mutation (alanine to serine) in the M2 gene at amino acid position 86. Previously discounted for not showing a phenotype in immortalized cell lines, this mutation contributes to both the attenuation and temperature sensitivity phenotypes of LAIV in primary human nasal epithelial cells. Furthermore, viruses encoding serine at M2 position 86 induced greater IFN-λ responses at early times post infection. Reversing mutations such as this, and otherwise altering LAIV’s ability to replicate in vivo, could result in an improved LAIV development strategy. Influenza viruses infect at and egress from the apical plasma membrane of airway epithelial cells. Accordingly, the virus transmembrane proteins, HA, NA, and M2, are all targeted to the apical plasma membrane ii and contribute to egress.
  • Role of the Interaction Between Large T Antigen and Rb Family Members in the Oncogenicity of JC Virus

    Role of the Interaction Between Large T Antigen and Rb Family Members in the Oncogenicity of JC Virus

    Oncogene (2006) 25, 5294–5301 & 2006 Nature Publishing Group All rights reserved 0950-9232/06 $30.00 www.nature.com/onc REVIEW Role of the interaction between large T antigen and Rb family members in the oncogenicity of JC virus V Caracciolo1, K Reiss2, K Khalili2, G De Falco1,3 and A Giordano1,3 1Department of Biology, Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA, USA; 2Department of Neuroscience, Center for Neurovirology, Temple University, Philadelphia, PA, USA and 3Department of Human Pathology and Oncology, University of Siena, Siena, Italy Human polyomaviruses (JC virus,BK virus and simian stimulating DNA replication (Cinatl and Doerr, virus 40) are causative agents of some human diseases 2001).Indeed, DNA viruses depend on the replication and,interestingly,are involved in processes of cell machinery of the host cell to complete viral replication transformation and oncogenesis. These viruses need the cycle.As the enzymes for DNA replication are present cell cycle machinery of the host cell to complete their in sufficient amounts only during the S phase of the replication; so they evolved mechanisms that can interfere cell cycle, the viruses have evolved mechanisms to with the growth control of infected cells and force them meet this requirement.They encode proteins that into DNA replication. The retinoblastoma family of interfere with growth control mechanisms of the infected proteins (pRb),which includes pRb/p105,p107 and cells, which allow them to drive cells from quiescence pRb2/p130,acts as one of the most important regulators to DNA replication (Nemethova et al., 2004). The of the G1/S transition of the cell cycle.