Tailored Microstructure of Colloidal Lipid Particles for Pickering Emulsions with Tunable Properties

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Tailored Microstructure of Colloidal Lipid Particles for Pickering Emulsions with Tunable Properties

Electronic Supplementary Material (ESI) for Soft Matter. This journal is © The Royal Society of Chemistry 2017 Supporting information. Tailored Microstructure of Colloidal Lipid Particles for Pickering Emulsions with Tunable Properties Anja A.J. Schröder1,2, Joris Sprakel2, Karin Schroën1, Claire C. Berton-Carabin1 1. Laboratory of Food Process Engineering, Wageningen University and Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands. 2. Physical Chemistry and Soft Matter, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands. Email addresses: [email protected], [email protected], [email protected], [email protected] S1 S1. Tripalmitin CLP dispersions produced with 0.5% w/w (left) and 1% w/w (right) Tween40 in the aqueous phase. S2. Fatty acid composition of palm stearin. Component name Percentage average C14:0 1.16 C16:0 82.18 C18:0 5.12 C18:1 9.03 C18:2 1.83 C18:3 0.02 C20:0 0.32 C22:0 0.25 C22:1 0.04 C24:0 0.05 S3. Hydrodynamic diameter (z-average) measured by dynamic light scattering, and, D[4,3], D[3,2], D10, D50 and D90 measured by static light scattering of CLPs composed of (a) tripalmitin, (b) tripalmitin/tricaprylin 4:1, (c) palm stearin. S2 Type of z-average PdI D[4,3] A D[3,2] D10 D50 D90 lipid (μm) (μm) (μm) (μm) (μm) (μm) Tripalmitin 0.162 ± 0.112 ± 0.130 ± 0.118 ± 0.082 ± 0.124 ± 0.184 ± 0.005 0.02 0.004 0.003 0.002 0.004 0.005 Tripalmitin/ 0.130 ± 0.148 ± 0.131 ± 0.117 ± 0.080 ± 0.124 ± 0.191 ± tricaprylin 0.011 0.02 0.003 0.001 0.004 0.002 0.010 (4:1) Palm 0.158 ± 0.199 ± 0.133 ± 0.119 ± 0.081 ± 0.127 ± 0.195 ± stearin 0.007 0.02 0.002 0.002 0.005 0.000 0.010 A B S4. TEM images of tripalmitin/tricaprylin (4:1 w/w) CLPs. S3 S5. TEM 3D volume model from the tomogram of tripalmitin CLPs. S4 Liquid (a.u) α energy β’ Gibbs β 30 40 50 60 70 Temperature (°C) S6. Structure models and Gibbs energy (G)–temperature relationship of three polymorphs of tripalmitin. Addapted from Sato, 2001 and Rønholt, Mortensen, & Knudsen, 2013. S5 I II III S7. Fluorescence recovery after photobleaching curve of an emulsion stabilized by tripalmitin CLPs, showing the normalized fluorescence intensity before bleaching (I), during bleaching (II) and during recovery (III). S8. Polarized light image of an O/W emulsion produced by high pressure homogenization with 50% w/w oil and 4.5% w/w tripalmitin CLPs. S6 S9. Polarized light image of an O/W emulsion produced wth a one-step emulsification process, where tripalmitin (1% w/w), sunflower oil (10% w/w) and an aqueous phase containing 2% w/w Tween 40 were directly manually homogenized. S7.

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