<p>Electronic Supplementary Material (ESI) for Soft Matter. This journal is © The Royal Society of Chemistry 2017 </p><p><strong>Supporting information. </strong></p><p>Tailored Microstructure of Colloidal Lipid Particles for Pickering Emulsions with Tunable Properties </p><p>Anja A.J. Schröder<sup style="top: -0.303em;">1,2</sup>, Joris Sprakel<sup style="top: -0.303em;">2</sup>, Karin Schroën<sup style="top: -0.303em;">1</sup>, Claire C. Berton-Carabin<sup style="top: -0.303em;">1 </sup><br>1. Laboratory&nbsp;of Food Process Engineering, Wageningen University and Research, Bornse <br>Weilanden 9, 6708 WG Wageningen, The Netherlands. <br>2. Physical&nbsp;Chemistry and Soft Matter, Wageningen University and Research, Stippeneng <br>4, 6708 WE Wageningen, The Netherlands. </p><p>Email addresses: <a href="mailto:[email protected]" target="_blank">[email protected]</a>, <a href="mailto:[email protected]" target="_blank">[email protected]</a>, <a href="mailto:[email protected]" target="_blank">[email protected]</a>, <a href="mailto:[email protected]" target="_blank">[email protected] </a></p><p>S1 </p><p>S1. Tripalmitin CLP dispersions produced with 0.5% w/w (left) and 1% w/w (right) Tween40 in the aqueous phase. </p><p>S2. Fatty acid composition of palm stearin. </p><p><strong>Component name </strong><br><strong>C14:0 </strong><br><strong>Percentage average </strong></p><p>1.16 <br>82.18 <br>5.12 9.03 1.83 0.02 0.32 0.25 0.04 0.05 </p><p><strong>C16:0 C18:0 C18:1 C18:2 C18:3 C20:0 C22:0 C22:1 C24:0 </strong></p><p>S3. Hydrodynamic diameter (z-average) measured by dynamic light scattering, and, D[4,3], D[3,2], D10, D50 and D90 measured by static light scattering of CLPs composed of (a) tripalmitin, (b) tripalmitin/tricaprylin 4:1, (c) palm stearin. </p><p>S2 </p><p>Type of lipid </p><ul style="display: flex;"><li style="flex:1">z-average </li><li style="flex:1">PdI </li><li style="flex:1">D[4,3] A </li></ul><p></p><p>(μm) </p><p>D[3,2] </p><p>(μm) </p><p>D10 </p><p>(μm) </p><p>D50 </p><p>(μm) </p><p>D90 </p><p></p><ul style="display: flex;"><li style="flex:1">(μm) </li><li style="flex:1">(μm) </li></ul><p></p><p>Tripalmitin 0.162&nbsp;± <br>0.005 <br>0.112 ±&nbsp;0.130 ± <br>0.02 0.004 <br>0.118 ± <br>0.003 <br>0.082 ± <br>0.002 <br>0.124 ± <br>0.004 <br>0.184 ± <br>0.005 </p><p></p><ul style="display: flex;"><li style="flex:1">Tripalmitin/ 0.130&nbsp;± </li><li style="flex:1">0.148 ±&nbsp;0.131 ± </li></ul><p>0.02 0.003 <br>0.117 ± <br>0.001 <br>0.080 ± <br>0.004 <br>0.124 ± <br>0.002 <br>0.191 ± </p><ul style="display: flex;"><li style="flex:1">0.010 </li><li style="flex:1">tricaprylin </li></ul><p>(4:1) <br>0.011 <br>Palm stearin <br>0.158 ± 0.007 </p><ul style="display: flex;"><li style="flex:1">0.199 ±&nbsp;0.133 ± </li><li style="flex:1">0.119 ± </li></ul><p>0.002 <br>0.081 ± <br>0.005 <br>0.127 ± <br>0.000 <br>0.195 ± </p><ul style="display: flex;"><li style="flex:1">0.010 </li><li style="flex:1">0.02 </li><li style="flex:1">0.002 </li></ul><p></p><p></p><ul style="display: flex;"><li style="flex:1"><strong>B</strong></li><li style="flex:1"><strong>A</strong></li></ul><p></p><p>S4. TEM images of tripalmitin/tricaprylin (4:1 w/w) CLPs. </p><p>S3 </p><p>S5. TEM 3D volume model from the tomogram of tripalmitin CLPs. </p><p>S4 </p><p><strong>Liquid </strong></p><p><strong>αβ’ β</strong></p><p></p><ul style="display: flex;"><li style="flex:1">30 </li><li style="flex:1">40 </li><li style="flex:1">50 </li><li style="flex:1">60 </li><li style="flex:1">70 </li></ul><p></p><p><strong>Temperature (°C) </strong></p><p>S6. Structure models and Gibbs energy (G)–temperature relationship of three polymorphs of tripalmitin. Addapted from Sato, 2001 and Rønholt, Mortensen, &amp; Knudsen, 2013. </p><p>S5 </p><p></p><ul style="display: flex;"><li style="flex:1"><em>I II </em></li><li style="flex:1"><em>III </em></li></ul><p></p><p>S7. Fluorescence recovery after photobleaching curve of an emulsion stabilized by tripalmitin CLPs, showing the normalized fluorescence intensity before bleaching (I), during bleaching (II) and during recovery (III). </p><p>S8. Polarized light image of an O/W emulsion produced by high pressure homogenization with 50% w/w oil and 4.5% w/w tripalmitin CLPs. </p><p>S6 </p><p>S9. Polarized light image of an O/W emulsion produced wth a one-step emulsification process, where tripalmitin (1% w/w), sunflower oil (10% w/w) and an aqueous phase containing 2% w/w Tween 40 were directly manually homogenized. </p><p>S7 </p>