OSBPL1A Antibody Cat

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OSBPL1A Antibody Cat. No.: 58-741 OSBPL1A Antibody Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse, Rat This OSBPL1A antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 736-763 amino acids from the C-terminal region of human OSBPL1A. TESTED APPLICATIONS: WB APPLICATIONS: For WB starting dilution is: 1:1000 PREDICTED MOLECULAR 108 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig October 1, 2021 1 https://www.prosci-inc.com/osbpl1a-antibody-58-741.html CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: OSBPL1A Oxysterol-binding protein-related protein 1, ORP-1, OSBP-related protein 1, OSBPL1A, ALTERNATE NAMES: ORP1, OSBP8, OSBPL1, OSBPL1B ACCESSION NO.: Q9BXW6 PROTEIN GI NO.: 20143880 GENE ID: 114876 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although BACKGROUND: some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. REFERENCES: 1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : 2) Hsu, Y.H., et al. PLoS Genet.(6), E1000977 (2010) : 3) Rocha, N., et al. J. Cell Biol. 185(7):1209-1225(2009) 4) Melzer, D., et al. PLoS Genet.(5), E1000072 (2008) : ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 1, 2021 2 https://www.prosci-inc.com/osbpl1a-antibody-58-741.html.

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S1 Supplemental Materials Supplemental Methods Supplemental Figure 1. Immune Phenotype of Mcd19 Targeted CAR T and Dose Titratio
Supplemental Materials Supplemental Methods Supplemental Figure 1. Immune phenotype of mCD19 targeted CAR T and dose titration of in vivo efficacy. Supplemental Figure 2. Gene expression of fluorescent-protein tagged CAR T cells. Supplemental Figure 3. Fluorescent protein tagged CAR T cells function similarly to non-tagged counterparts. Supplemental Figure 4. Transduction efficiency and immune phenotype of mCD19 targeted CAR T cells for survival study (Figure 2D). Supplemental Figure 5. Transduction efficiency and immune phenotype of CAR T cells used in irradiated CAR T study (Fig. 3B-C). Supplemental Figure 6. Differential gene expression of CD4+ m19-humBBz CAR T cells. Supplemental Figure 7. CAR expression and CD4/CD8 subsets of human CD19 targeted CAR T cells for Figure 5E-G. Supplemental Figure 8. Transduction efficiency and immune phenotype of mCD19 targeted wild type (WT) and TRAF1-/- CAR T cells used for in vivo study (Figure 6D). Supplemental Figure 9. Mutated m19-musBBz CAR T cells have increased NF-κB signaling, improved cytokine production, anti-apoptosis, and in vivo function. Supplemental Figure 10. TRAF and CAR co-expression in human CD19-targeted CAR T cells. Supplemental Figure 11. TRAF2 over-expressed h19BBz CAR T cells show similar in vivo efficacy to h19BBz CAR T cells in an aggressive leukemia model. S1 Supplemental Table 1. Probesets increased in m19z and m1928z vs m19-musBBz CAR T cells. Supplemental Table 2. Probesets increased in m19-musBBz vs m19z and m1928z CAR T cells. Supplemental Table 3. Probesets differentially expressed in m19z vs m19-musBBz CAR T cells. Supplemental Table 4. Probesets differentially expressed in m1928z vs m19-musBBz CAR T cells.
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bioRxiv preprint doi: https://doi.org/10.1101/679456; this version posted June 21, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Animal complexity and information flow 1 1 2 3 4 5 Variation in protein coding genes identifies information flow as a contributor to 6 animal complexity 7 8 Jack Dean, Daniela Lopes Cardoso and Colin Sharpe* 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Institute of Biological and Biomedical Sciences 25 School of Biological Science 26 University of Portsmouth, 27 Portsmouth, UK 28 PO16 7YH 29 30 * Author for correspondence 31 [email protected] 32 33 Orcid numbers: 34 DLC: 0000-0003-2683-1745 35 CS: 0000-0002-5022-0840 36 37 38 39 40 41 42 43 44 45 46 47 48 49 Abstract bioRxiv preprint doi: https://doi.org/10.1101/679456; this version posted June 21, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Animal complexity and information flow 2 1 Across the metazoans there is a trend towards greater organismal complexity. How 2 complexity is generated, however, is uncertain. Since C.elegans and humans have 3 approximately the same number of genes, the explanation will depend on how genes are 4 used, rather than their absolute number.
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Molecular Cancer Therapeutics 151 Distinct gene expression patterns in a tamoxifen-sensitive human mammary carcinoma xenograft and its tamoxifen-resistant subline MaCa 3366/TAM Michael Becker,1 Anette Sommer,2 of acquired tamoxifen resistance. Finally, genes whose Jo¨rn R. Kra¨tzschmar,2 Henrik Seidel,2 expression profiles are distinctly changed between the Hans-Dieter Pohlenz,2 and Iduna Fichtner1 two xenograft lines will be further evaluated as potential targets for diagnostic or therapeutic approaches of 1 Max-Delbrueck-Center for Molecular Medicine, Experimental tamoxifen-resistant breast cancer. [Mol Cancer Ther Pharmacology, and 2Research Laboratories, Schering AG, Berlin, Germany 2005;4(1):151–68] Abstract Introduction The reasons why human mammary tumors become Breast cancer is the most common type of cancer in women resistant to tamoxifen therapy are mainly unknown. of the Western world. Due to advances in early detection Changes in gene expression may occur as cells acquire and treatment, breast cancer survival rates have increased resistance to antiestrogens. We therefore undertook a com- markedly over the past decades. After surgery, estrogen parative gene expression analysis of tamoxifen-sensitive receptor a (ERa)–positive breast cancer is usually treated and tamoxifen-resistant human breast cancer in vivo with endocrine therapy. Tamoxifen, a nonsteroidal anti- models using Affymetrix oligonucleotide arrays to analyze estrogen, also termed selective estrogen receptor modula- differential gene expression. Total RNAs from the tamox- tor, is the first-line therapy for premenopausal and, until ifen-sensitive patient-derived mammary carcinoma xeno- recently, also for postmenopausal hormone receptor– graft MaCa 3366 and the tamoxifen-resistant model MaCa positive women (1).
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View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Progress in Lipid Research 61 (2016) 30–39 Contents lists available at ScienceDirect Progress in Lipid Research journal homepage: www.elsevier.com/locate/plipres Review The orchestra of lipid-transfer proteins at the crossroads between metabolism and signaling Antonella Chiapparino a, Kenji Maeda a,1, Denes Turei a,b, Julio Saez-Rodriguez b,2,Anne-ClaudeGavina,c,⁎ a European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1, D-69117 Heidelberg, Germany b European Molecular Biology Laboratory (EMBL), European Bioinformatics Institute (EBI), Cambridge CB10 1SD, UK c European Molecular Biology Laboratory (EMBL), Molecular Medicine Partnership Unit (MMPU), Meyerhofstrasse 1, D-69117 Heidelberg, Germany article info abstract Article history: Within the eukaryotic cell, more than 1000 species of lipids deﬁne a series of membranes essential for cell func- Received 29 September 2015 tion. Tightly controlled systems of lipid transport underlie the proper spatiotemporal distribution of membrane Accepted 15 October 2015 lipids, the coordination of spatially separated lipid metabolic pathways, and lipid signaling mediated by soluble Available online 1 December 2015 proteins that may be localized some distance away from membranes. Alongside the well-established vesicular transport of lipids, non-vesicular transport mediated by a group of proteins referred to as lipid-transfer proteins Keywords: (LTPs) is emerging as a key mechanism of lipid transport in a broad range of biological processes. More than a Signaling lipid Biological membranes hundred LTPs exist in humans and these can be divided into at least ten protein families.
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Goat Anti-ORP1 Antibody Peptide-Affinity Purified Goat Antibody Catalog # Af1758a
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Goat Anti-ORP1 Antibody Peptide-affinity purified goat antibody Catalog # AF1758a Specification Goat Anti-ORP1 Antibody - Product Information Application WB Primary Accession Q9BXW6 Other Accession NP_542164, 114876, 64291 (mouse), 259221 (rat) Reactivity Human Predicted Mouse, Rat, Dog, Cow Host Goat Clonality Polyclonal Concentration 100ug/200ul Isotype IgG Calculated MW 108470 AF1758a staining (0.5 µg/ml) of Human Goat Anti-ORP1 Antibody - Additional Information Muscle lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Gene ID 114876 Detected by western blot using chemiluminescence. Other Names Oxysterol-binding protein-related protein 1, ORP-1, OSBP-related protein 1, OSBPL1A, Goat Anti-ORP1 Antibody - Background ORP1, OSBP8, OSBPL1, OSBPL1B This gene encodes a member of the Format oxysterol-binding protein (OSBP) family, a 0.5 mg IgG/ml in Tris saline (20mM Tris group of intracellular lipid receptors. Most pH7.3, 150mM NaCl), 0.02% sodium azide, members contain an N-terminal pleckstrin with 0.5% bovine serum albumin homology domain and a highly conserved Storage C-terminal OSBP-like sterol-binding domain, Maintain refrigerated at 2-8°C for up to 6 although some members contain only the months. For long term storage store at sterol-binding domain. Transcript variants -20°C in small aliquots to prevent derived from alternative promoter usage freeze-thaw cycles. and/or alternative splicing exist; they encode different isoforms. Precautions Goat Anti-ORP1 Antibody is for research use Goat Anti-ORP1 Antibody - References only and not for use in diagnostic or therapeutic procedures.
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bioRxiv preprint doi: https://doi.org/10.1101/2020.02.24.962738; this version posted February 26, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 TITLE: PCV2 aggravated SS2 infection directly showed from differentially 2 expressed proteome of PK15 cells. 3 Tan Jimin1,2, Xiao Qi2,3,4, Li Xingguang5, Fan Hongjie1*, He Kongwang2,3,4* 4 5 6 1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China 7 2Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation 8 Base Ministry of Science and Technology, Nanjing, China 9 3Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences-Key 10 Laboratory of Veterinary Biological Engineering and Technology, Ministry of 11 Agriculture and Rural Affairs, Nanjing, China 12 4Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal 13 Infectious Disease and Zoonose, Yangzhou University, Yangzhou, China 14 5Hubei Engineering Research Center of Viral Vector, Wuhan University of 15 Bioengineering, Wuhan 430415, China. 16 Correspondence: 17 Fan Hongjie ([email protected]) 18 He Kongwang ([email protected]) 19 20 21 Running title: PCV2 and SS2 co-infection proteomic study of Porcine Kidney cell 15 22 23 bioRxiv preprint doi: https://doi.org/10.1101/2020.02.24.962738; this version posted February 26, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.
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