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Custom Peptide Microarray Service Custom Peptide Microarray Service MULTIPLEX PROTEIN KINASE ASSAYS Perform protein kinase reactions on arrays of comprehensive sets of substrates. Measure kinase activity and inhibitor effects. Obtain hundreds of reaction profiles and curves in parallel for parameters such as VMAX, KM, and KI. Use of microarray titer plate format will increase your workflow by at least 40-fold that of a single microtiter plate. LARGE SCALE HIGH RESOLUTION EPITOPE MAPPING Perform large scale parallel epitope mapping from pathogens. Identify high affinity antibody binding peptides. Screen sensitive antibody-detection peptides. Detect autoantibodies/biomarkers from serum or biological samples, determine Kd (binding affinity) of hundreds of binding peptides, obtain titration curves for antibodies. PATHWAY PHOSPHOPEPTIDE-BINDING PROTEIN STUDIES Profile SH2-containing proteins in complex biological samples by an array of phosphopeptides which are carefully curated from literature. Screen effective SH2 binding inhibitors and measure inhibitory activities. Screen signature phosphopeptides for protein capture as a tool for signaling pathway characterization. PEPTIDES AND PEPTIDOMIMETICS FOR PROTEIN CAPTURE Screen biomarkers (proteins and peptide binding targets) from biological samples such as blood serum or cell lysates. Use novel protein capture peptides to detect and profile on the same chip platform a wide range of molecular biomarkers: signaling protein kinases, pathway phosphoprotein-binding and domain-binding proteins, immuno-responsive antibodies, metabolites and metabolic molecules, and other small molecules. THERAPEUTIC SYNTHETIC PEPTIDE SCREENING Rapidly design, synthesize, and screen diverse peptides, peptide analogs, and peptides modified with glyco-units against important therapeutic targets. www.lcsciences.com 1-888-528-8818 Cost Effective 1-Stop Proteomics Solution Comprehensive Sample to Data Service This is a comprehensive PROTEOMICS service - send us your sample - we’ll manufacture a peptide microarray of standard content or synthesize a custom designed array, carry out the sample assays you request, perform data collection and analysis, and deliver a results report to you. Our cost effective 1-stop solution can save you tremendous time and money and the use of a microarray titer plate format will increase your workflow to 40X that of a single microtiter plate. High Throughput Format Thousands of different peptide sequences are synthesized on one array (1 cm2) 1 peptide microarray titer plate = 40 X 96-well microtiter plates. Target thousands of peptide sequences at once - perform thousands of multiplex parallel assays at addressable chip locations. Generate hundreds of binding curves in a single experiment. Scaleable to proteomic scale. Pico-Liter Peptide Microarray on a µParaflo® microfluidics microchip Innovative Microfluidic Array Platform Peptides are synthesized by conventional t-boc chemistry on a proprietary µParaflo® microfluidic biochip. The microfluidic technology ensures: An engineerable, reproducible, automatable, and scalable proteomic platform. Uniform distribution of the sample solutions on the array. Efficient sample-peptide contact. Rapid and straightforward optimization. Low background and specific binding by stringency wash processes. Enclosed environment for sample stability and free of contamination. Dye oxidation and deterioration are prevented. Sensitive fluorescence detection. Microarray titer plate format increases your workflow 40X that of a single microtiter plate. www.lcsciences.com 1-888-528-8818 30-10B-02 Protein Assay on a Peptide Microarray Quantitative Results Quantitative Results Estimated Order Kd #(1=we (Releaive akest Generate information about specific probe sequences - sequences AU) binding)10000Series# 10000 can be defined to each single amino acid residue and the PTP_N3235 0.54 9 8 interpretation of the assay results are taken directly from digitalPTPN11-1121 0.60 8 9 PTPN11-1157 0.63 7 11 image read out of an addressable microarray. PTPN11-1178 0.63 7 7500 12 7500 Make use of convenient, safe, sensitive fluorescence detection.PTP_N0218 0.70 6 7 PTPN6-1211 0.90 5 13 Achieve reliable and quantifiable results through the highlyEGFR_Y1197 stringent 1.01 4 2 50% Binding Strength50% Binding Strength 5000 5000 design and use of negative and positive control references.CagAD1 False 1.11 3 1 positive readings are minimized. STAT6-1684 1.11 3 18 GABI_Y627 1.39 2 3 IRS1_Y1222 1.39 2 5 2500 2500 SRC-1543 1.39 2 15 SRC-1565 1.47 2 16 GRB2-0521 1.47 1 4 PDGFRB_Y579 1.47 1 0 6 0 PTPN11-1122 1.49 1 0.0110 0.01 0.1 0.1 1 1 10 10 SRC-1566 1.49 1 17 Ultra-low Sample Consumption Miniaturized array features reduces consumption of valuable samples to the sub-nanoliter to picoliter level per reaction. Perform thousands of peptide assays per array using only sub-µg of protein. Customer Designed Sequences Flexible Design Completely customizable design of peptides according to your needs. All arrays are synthesized to order. Choose from our standard microarray content, customer specified sequences and layout, or sequences custom designed by LC Sciences. Quickly revise microarray design and content to keep experiments moving forward based on previous results. Conventional t-boc synthesis chemistry is completely compatible with amino acid analogs. Incorporate analogs to expand the range of applications. www.lcsciences.com 1-888-528-8818 www.lcsciences.com 1-888-528-8818 30-10B-03 Innovative Microfluidic Array Platform Microfluidic Array Platform These are not spotted arrays! A proprietary µParaflo® microfluidic biochip is used and custom peptide sequences are synthesized on-chip. The microfluidic technology produces a uniform distribution of the sample solutions on the array, ensures efficient sample-peptide contact and enhances binding reactions and stringency wash processes. The microarray chip consists of thousands of three-dimensional chambers and is a closed system. Under these conditions multiplex protein assays are carried out in a way much like in thousands of pico-liter tubes, enclosure keeps the proteins in a stable environment, in solution and protected from air– oxidation/contamination. The miniaturized system provides automation, sample/reagent-savings and simplicity in operation. In situ Parallel Synthesis In situ peptide synthesis using PGA (photogenerated acid) coupled with conventional t-boc chemistry and a programmable process means high probe quality, tight process control, and complete content flexibility. Our advanced manufacturing process ensures highly uniform spots and high reproducibility from array to array and yet permits total customization of contents on each individual array. In comparison, spotted microarrays tend to suffer from poor spot uniformity and large spot to spot and array to array variations, which lead to large data deviations. The spotting process requires significant up-front investment for peptide libraries and spotting equipment and thus is inflexible for customization. Complete Control of Peptide Microarray Quality We ensure reliable and quantifiable results through the use of multiple negative and positive controls as well as reference peptides. Multiple QC measures are implemented at various stages of array manufacturing and assay processes. The evaluation of signal intensities of these control spots allows assessment of spot uniformity, cross-array spot-to-spot uniformity, and binding specificities and strength. Comprehensive and Current Peptide Content Each peptide of a predetermined sequence is synthesized on a long spacer. The peptide sequences may be standard catalog content (from LC Sciences’ library containing database and/or literature validated peptides) or custom content (customer defined sequences). We use the most updated information on proteins for peptide microarray design to deliver arrays of comprehensive, systematic and up to date content. Sequence repeats are used on each array to allow statistical analysis of the data. Multiple-Time Repetitive Assays on the Same Chip Peptide microarrays can be used for time-, protein/antibody/enzyme concentration-, or co-factor/inhibitor-dependent measurements. The array can be used multiple times, each time assay is carried out and assay signals are collected by fluorescence scan. These assays generate a very large set of data for proteomic assays and valuable information which would take weeks and months to produce using a conventional single protein/antibody/enzyme assay kit. www.lcsciences.com 1-888-528-8818 30-3010B-10B-04- 04 Pico-liter Scale Microarray Titer Plates Peptide Concentration Variation On-Chip Our unique synthesis chemistry makes it possible to vary the substrate density of reaction sites to create a gradient across the array. This array can generate the same amount of data equivalent to that of 40 conventional microtiter plates! This form of miniaturized multiplex parallel protein/antibody assays saves assay samples, reagents, labor and time in generating binding affinity or enzymatic reaction curves with a single experiment, allowing cross comparison of the systems assayed. The use of comparisons provides a more comprehensive picture and more reliable results. Close up view of a µParaFlo® biochip Plate 1 Seq 001 002 003 004 005 006 007 008 Plate 2 Repeat Seq 011 012 013 014 015 016 017 018 Plate 3 Spots Density Repeat Seq 101 102 103 104 105 106 107 108 Spots Density Repeat Peptide Concentration Variation Across the Microarray Titer Plate Spots Density 1 Microarray = 40
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