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Pepperprint Product Handout PepSlide® Analyzer PepSlide® Analyzer Specifications Quantify and analyze your peptide microarray data with our intuitive and easy-to-use software solution: • use microarray scanners‘ TIFF images and load design data from GAL files or PepSlide® PSF files • efficient tools for image processing, array alignment and spot finding • perfectly adapted to the PEPperCHIP® platform • full compatibility with Excel PEPperPRINT GmbH • recognition of custom spot sizes and morphologies ® ® Rischerstraße 12 Fon: +49 (0) 62 21 72 64 489 pepperchip@pepperprint.com PePperChiP PePperMap • state of the art background correction D-69123 Heidelberg Fax: +49 (0)6221 7264475 www.pepperprint.com High-Content Peptide Microarrays Epitope Mapping & Serum Profiling Services • cost-effective alternative to GenePix® Pro Custom Peptide Synthesis Peptide Specifications • HPLC purified custom peptides (95% purity on demand) • 1 mg (€ 11/amino acid), 5 mg (€ 22/amino acid) and 10 mg (€ 33/amino acid) batches • 16, 96 and 384-well small scale peptide libraries • linear and cyclic peptides • various modifications available (phosphorylation, PEGylation, biotinylation, acetylation, methylation etc.)PEPperCHIP® PEPperCHIP® • useful for competition and enzymatic assays, peptideGet access to custom and Get access to custom and immobilization and peptide array spotting standard peptide microarrays standard peptide microarrays with up to 9,000 features per with up to 9,000 features per Please inquire for other peptide purities, quantities and modifications. chip in a uniquely flexible and chip in a uniquely flexible and cost-effective manner. cost-effective manner. PEPperMAP® High Content Peptide Microarrays PEPperMAP® High Content Peptide Microarrays Benefit from our new peptide Epitope Mapping • Serum Profiling • Peptide Engineering Benefit from our new peptide Epitope Mapping • Serum Profiling • Peptide Engineering microarray platform and let microarray platform and let PEPperPRINT's experts get PEPperPRINT's experts get the results for you. the results for you. Pe PperChiP® Pe PperChiP® Applications PePperMAP® Services P E perCHIP® Specifications Epitope Mapping P E perMA ® Specifications • custom and off-the-shelf peptide microarrays • characterization of mono- & polyclonal antibodies Benefit from PEPperPRINT’s state-of-the-art and sera ® • up to 9,000 features per chip peptide microarray platform PEPperCHIP without • proteins are translated into overlapping peptides with any need for time, manpower or technical infra- • thorough customization covering any protein or maximum peptide-peptide overlap structure. peptide collection • custom peptide mapping contents from single to • fully adjusted to your research Just send us your antibody or serum sample and multiple antigens • standard slide formats with wide chip reader let PEPperPRINT’s experts get the results for you. compatibility • variable peptide lengths and overlap We are pleased to provide you with standard • GenePix® Pro and PepSlide® Analyzer support Epitope mapping of a polyclonal antibody against two • up to five one-by-one tests per chip possible PEPperCHIP® - PEPperPRINT’s new peptide microarray antigens as overlapping peptides in double spots (red; stained epitope mapping services and custom serum PEPperMAP® - The most convenient way to benefit from • most cost effective solutions platform with custom and standard contents control peptides frame the antigen-derived peptides) • minimal sample consumption profiling solutions for your research. PEPperPRINT’s new peptide microarray platform. P E perCHIP® Variants Immune Profiling of Blood Sera Epitope mapping P E perMA ® Elements of a monoclonal Benefit from custom contents adjusted to your research: • fingerprint analysis of immune responses in sera linked with infection, antibody with a • a custom peptide microarray with your antigen vaccination, oncogenesis, autoimmune diseases single epitope (left) as overlapping peptides • display your antigen(s) as overlapping peptides for epitope mapping compared to a identification of differential signatures in patient and control groups • polyclonal sample • all immunoassays with your antibody or serum • use more than 4,100 random peptides for mimotope discovery and (bottom) with two sample and secondary antibodies serum signaturing • custom and off-the-shelf contents available covering various indications, epitopes. organisms, strains, subtypes and antigen collection • control assays of Flag and HA control peptides • decode known peptides and epitopes with full permutation scans • high reproducibility and data consistency for statistical analysis of • read-out of microarray data • translate custom peptide lists into peptide microarrays microarray data • data quantification and evaluation • include multiple array copies per chip for one-by-one tests • material saving with suited PEPperCHIP® Incubation Trays Custom solutions from single • numerous standard contents available (antigens from EBV, HBV, H1N1, Custom peptide microarrays with more than 8,000 features for the discovery of serum signatures Green/red frames: Staining of routinely implemented HA • detailed report with protocols, experimental antigens to full proteome sets. HCV, Dengue virus, HPV, melanoma etc.) (left) and full permutation scans of a known antibody epitope (right) and Flag control peptides. details and results P E perCHIP® Accessories Antigen/Epitope/Mimotope Discovery P E perMA ® Options • PEPperCHIP® Incubation Trays with 3/1, 3/2, 3/3, 3/4, 3/5, 3/16 and 3/(3,4,5) well formats Access high content microarrays with up to 25,000 peptides for According to your demand, we offer various epitope mapping options from only € 675 on as well as ® 33 one-by-one assays with multiple array copies the discovery and development of: completely customized PEPperMAP solutions. 33 minimal sample consumption • new epitopes and mimotopes • Type 1 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into 13mer peptides with a peptide-peptide overlap of 12 amino acids 33 enables shaking incubation • serum biomarker and antibody signatures • Type 2 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into active antigens in complex protein collections • PEPperCHIP® Staining Kit with labeled anti-HA and anti-Flag • 10, 12 and 15mer peptides with a peptide-peptide overlap of 9, 11 and 14 amino acids antibodies for control assays • protein target binders and affinity tags • Type 1+/2+ Mapping with success-related fees ® • PepSlide Analyzer for software-assisted image analysis of • peptide hit discovery and lead optimization • Type 3 Mapping: Full epitope scans with thorough sequence permutations peptide microarray data High content PEPperCHIP® with 25,000 peptides for antigen, epitope, mimotope Please inquire or download our Product Catalog (www.pepperprint.com/ PEPperPRINT_Catalog.pdf) for a • GenePix® Pro support with GAL files PEPperCHIP® Incubation Tray (3/4 well format) and target binder discovery (right) comprehensive product overview and pricing information. Pe PperChiP® Pe PperChiP® Applications PePperMAP® Services P E perCHIP® Specifications Epitope Mapping P E perMA ® Specifications • custom and off-the-shelf peptide microarrays • characterization of mono- & polyclonal antibodies Benefit from PEPperPRINT’s state-of-the-art and sera ® • up to 9,000 features per chip peptide microarray platform PEPperCHIP without • proteins are translated into overlapping peptides with any need for time, manpower or technical infra- • thorough customization covering any protein or maximum peptide-peptide overlap structure. peptide collection • custom peptide mapping contents from single to • fully adjusted to your research Just send us your antibody or serum sample and multiple antigens • standard slide formats with wide chip reader let PEPperPRINT’s experts get the results for you. compatibility • variable peptide lengths and overlap We are pleased to provide you with standard • GenePix® Pro and PepSlide® Analyzer support Epitope mapping of a polyclonal antibody against two • up to five one-by-one tests per chip possible PEPperCHIP® - PEPperPRINT’s new peptide microarray antigens as overlapping peptides in double spots (red; stained epitope mapping services and custom serum PEPperMAP® - The most convenient way to benefit from • most cost effective solutions platform with custom and standard contents control peptides frame the antigen-derived peptides) • minimal sample consumption profiling solutions for your research. PEPperPRINT’s new peptide microarray platform. P E perCHIP® Variants Immune Profiling of Blood Sera Epitope mapping P E perMA ® Elements of a monoclonal Benefit from custom contents adjusted to your research: • fingerprint analysis of immune responses in sera linked with infection, antibody with a • a custom peptide microarray with your antigen vaccination, oncogenesis, autoimmune diseases single epitope (left) as overlapping peptides • display your antigen(s) as overlapping peptides for epitope mapping compared to a identification of differential signatures in patient and control groups • polyclonal sample • all immunoassays with your antibody or serum • use more than 4,100 random peptides for mimotope discovery and (bottom) with two sample and secondary antibodies serum signaturing • custom and off-the-shelf contents available covering various indications, epitopes. organisms, strains, subtypes and antigen collection • control assays of Flag and HA control peptides • decode known peptides
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