DOCSLIB.ORG

Pepperprint Product Handout

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Pepperprint Product Handout PepSlide® Analyzer PepSlide® Analyzer Specifications Quantify and analyze your peptide microarray data with our intuitive and easy-to-use software solution: • use microarray scanners‘ TIFF images and load design data from GAL files or PepSlide® PSF files • efficient tools for image processing, array alignment and spot finding • perfectly adapted to the PEPperCHIP® platform • full compatibility with Excel PEPperPRINT GmbH • recognition of custom spot sizes and morphologies ® ® Rischerstraße 12 Fon: +49 (0) 62 21 72 64 489 [email protected] PePperChiP PePperMap • state of the art background correction D-69123 Heidelberg Fax: +49 (0)6221 7264475 www.pepperprint.com High-Content Peptide Microarrays Epitope Mapping & Serum Profiling Services • cost-effective alternative to GenePix® Pro Custom Peptide Synthesis Peptide Specifications • HPLC purified custom peptides (95% purity on demand) • 1 mg (€ 11/amino acid), 5 mg (€ 22/amino acid) and 10 mg (€ 33/amino acid) batches • 16, 96 and 384-well small scale peptide libraries • linear and cyclic peptides • various modifications available (phosphorylation, PEGylation, biotinylation, acetylation, methylation etc.)PEPperCHIP® PEPperCHIP® • useful for competition and enzymatic assays, peptideGet access to custom and Get access to custom and immobilization and peptide array spotting standard peptide microarrays standard peptide microarrays with up to 9,000 features per with up to 9,000 features per Please inquire for other peptide purities, quantities and modifications. chip in a uniquely flexible and chip in a uniquely flexible and cost-effective manner. cost-effective manner. PEPperMAP® High Content Peptide Microarrays PEPperMAP® High Content Peptide Microarrays Benefit from our new peptide Epitope Mapping • Serum Profiling • Peptide Engineering Benefit from our new peptide Epitope Mapping • Serum Profiling • Peptide Engineering microarray platform and let microarray platform and let PEPperPRINT's experts get PEPperPRINT's experts get the results for you. the results for you. Pe PperChiP® Pe PperChiP® Applications PePperMAP® Services P E perCHIP® Specifications Epitope Mapping P E perMA ® Specifications • custom and off-the-shelf peptide microarrays • characterization of mono- & polyclonal antibodies Benefit from PEPperPRINT’s state-of-the-art and sera ® • up to 9,000 features per chip peptide microarray platform PEPperCHIP without • proteins are translated into overlapping peptides with any need for time, manpower or technical infra- • thorough customization covering any protein or maximum peptide-peptide overlap structure. peptide collection • custom peptide mapping contents from single to • fully adjusted to your research Just send us your antibody or serum sample and multiple antigens • standard slide formats with wide chip reader let PEPperPRINT’s experts get the results for you. compatibility • variable peptide lengths and overlap We are pleased to provide you with standard • GenePix® Pro and PepSlide® Analyzer support Epitope mapping of a polyclonal antibody against two • up to five one-by-one tests per chip possible PEPperCHIP® - PEPperPRINT’s new peptide microarray antigens as overlapping peptides in double spots (red; stained epitope mapping services and custom serum PEPperMAP® - The most convenient way to benefit from • most cost effective solutions platform with custom and standard contents control peptides frame the antigen-derived peptides) • minimal sample consumption profiling solutions for your research. PEPperPRINT’s new peptide microarray platform. P E perCHIP® Variants Immune Profiling of Blood Sera Epitope mapping P E perMA ® Elements of a monoclonal Benefit from custom contents adjusted to your research: • fingerprint analysis of immune responses in sera linked with infection, antibody with a • a custom peptide microarray with your antigen vaccination, oncogenesis, autoimmune diseases single epitope (left) as overlapping peptides • display your antigen(s) as overlapping peptides for epitope mapping compared to a identification of differential signatures in patient and control groups • polyclonal sample • all immunoassays with your antibody or serum • use more than 4,100 random peptides for mimotope discovery and (bottom) with two sample and secondary antibodies serum signaturing • custom and off-the-shelf contents available covering various indications, epitopes. organisms, strains, subtypes and antigen collection • control assays of Flag and HA control peptides • decode known peptides and epitopes with full permutation scans • high reproducibility and data consistency for statistical analysis of • read-out of microarray data • translate custom peptide lists into peptide microarrays microarray data • data quantification and evaluation • include multiple array copies per chip for one-by-one tests • material saving with suited PEPperCHIP® Incubation Trays Custom solutions from single • numerous standard contents available (antigens from EBV, HBV, H1N1, Custom peptide microarrays with more than 8,000 features for the discovery of serum signatures Green/red frames: Staining of routinely implemented HA • detailed report with protocols, experimental antigens to full proteome sets. HCV, Dengue virus, HPV, melanoma etc.) (left) and full permutation scans of a known antibody epitope (right) and Flag control peptides. details and results P E perCHIP® Accessories Antigen/Epitope/Mimotope Discovery P E perMA ® Options • PEPperCHIP® Incubation Trays with 3/1, 3/2, 3/3, 3/4, 3/5, 3/16 and 3/(3,4,5) well formats Access high content microarrays with up to 25,000 peptides for According to your demand, we offer various epitope mapping options from only € 675 on as well as ® 33 one-by-one assays with multiple array copies the discovery and development of: completely customized PEPperMAP solutions. 33 minimal sample consumption • new epitopes and mimotopes • Type 1 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into 13mer peptides with a peptide-peptide overlap of 12 amino acids 33 enables shaking incubation • serum biomarker and antibody signatures • Type 2 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into active antigens in complex protein collections • PEPperCHIP® Staining Kit with labeled anti-HA and anti-Flag • 10, 12 and 15mer peptides with a peptide-peptide overlap of 9, 11 and 14 amino acids antibodies for control assays • protein target binders and affinity tags • Type 1+/2+ Mapping with success-related fees ® • PepSlide Analyzer for software-assisted image analysis of • peptide hit discovery and lead optimization • Type 3 Mapping: Full epitope scans with thorough sequence permutations peptide microarray data High content PEPperCHIP® with 25,000 peptides for antigen, epitope, mimotope Please inquire or download our Product Catalog (www.pepperprint.com/ PEPperPRINT_Catalog.pdf) for a • GenePix® Pro support with GAL files PEPperCHIP® Incubation Tray (3/4 well format) and target binder discovery (right) comprehensive product overview and pricing information. Pe PperChiP® Pe PperChiP® Applications PePperMAP® Services P E perCHIP® Specifications Epitope Mapping P E perMA ® Specifications • custom and off-the-shelf peptide microarrays • characterization of mono- & polyclonal antibodies Benefit from PEPperPRINT’s state-of-the-art and sera ® • up to 9,000 features per chip peptide microarray platform PEPperCHIP without • proteins are translated into overlapping peptides with any need for time, manpower or technical infra- • thorough customization covering any protein or maximum peptide-peptide overlap structure. peptide collection • custom peptide mapping contents from single to • fully adjusted to your research Just send us your antibody or serum sample and multiple antigens • standard slide formats with wide chip reader let PEPperPRINT’s experts get the results for you. compatibility • variable peptide lengths and overlap We are pleased to provide you with standard • GenePix® Pro and PepSlide® Analyzer support Epitope mapping of a polyclonal antibody against two • up to five one-by-one tests per chip possible PEPperCHIP® - PEPperPRINT’s new peptide microarray antigens as overlapping peptides in double spots (red; stained epitope mapping services and custom serum PEPperMAP® - The most convenient way to benefit from • most cost effective solutions platform with custom and standard contents control peptides frame the antigen-derived peptides) • minimal sample consumption profiling solutions for your research. PEPperPRINT’s new peptide microarray platform. P E perCHIP® Variants Immune Profiling of Blood Sera Epitope mapping P E perMA ® Elements of a monoclonal Benefit from custom contents adjusted to your research: • fingerprint analysis of immune responses in sera linked with infection, antibody with a • a custom peptide microarray with your antigen vaccination, oncogenesis, autoimmune diseases single epitope (left) as overlapping peptides • display your antigen(s) as overlapping peptides for epitope mapping compared to a identification of differential signatures in patient and control groups • polyclonal sample • all immunoassays with your antibody or serum • use more than 4,100 random peptides for mimotope discovery and (bottom) with two sample and secondary antibodies serum signaturing • custom and off-the-shelf contents available covering various indications, epitopes. organisms, strains, subtypes and antigen collection • control assays of Flag and HA control peptides • decode known peptides
Load more

Recommended publications
  • Inkjet Printing for the Production of Protein Microarrays Mcwilliam Et Al Mimb Protein Microarrays Chapter 21
    Methods in Molecular Biology Protein Microarrays Chapter 21: Inkjet Printing for the Production of Protein Microarrays Iain McWilliam*, Marisa Chong Kwan, Duncan Hall Arrayjet Ltd., MIC, Roslin, EH25 9RE, UK * Corresponding Author Keywords i. Inkjet ii. Microarrayer iii. Protein microarray iv. Printing buffer v. Diagnostic microarray vi. Non-contact vii. JetSpyder viii. JetGuard ix. Microarray production x. Antibody microarray Introduction Since their inception in 1995, microarrays have been the icon of the ‘omics revolution. Whereas DNA can be printed onto a wide range of surface chemistries with a wide range of buffers, proteins must be printed in buffers which protect them and onto substrates which maintain their structural integrity, binding sites and activity. Common buffers for protein storage contain cryoprotectorants (e.g. glycerol (1) or ethylene glycol) which add to the often viscous nature of protein solutions, so the ability of a microarrayer to print such solutions without extensive modification is a distinct advantage when producing protein microarrays. The most popular substrates for protein microarrays, designed specifically with structural protection in mind, are made with thin nitrocellulose or hydrogel coatings and are fragile themselves, which encourages the use of a non—contact method of printing. When addressing production throughput requirements, the ability to handle multiple samples simultaneously and to print those samples quickly on—the—fly is important to minimise production timescales. The technology should also be scalable and robust enough to accommodate a shift from R+D level production to full—scale, manufacturing levels of production. Arrayjet has used the key factors of being able to print potentially viscous protein solutions with a non—contact printing method to develop a range of microarrayers, all centred around an industrially proven inkjet printhead, which are highly suited to printing high quality protein microarrays at all levels of production.
    [Show full text]
  • Process Development for Manufacturing Stochastic Peptide Microarrays
    Process Development for Manufacturing Stochastic Peptide Microarrays Zur Erlangung des akademischen Grades eines DOKTORS DER INGENIEURSWISSENSCHAFTEN (Dr.-Ing.) von der KIT-Fakultät für Maschinenbau des Karlsruher Instituts für Technologie (KIT) angenommene DISSERTATION von M.Sc. Roman Popov Tag der mündlichen Prüfung: 20. März 2018 Hauptreferent: apl. Prof. Dr. Alexander Nesterov-Müller Korreferenten: Prof. Dr. Jan Korvink Prof. Dr.-Ing. Matthias Franzreb Dieses Werk ist unter einer Creative Commons Namensnennung – Nicht-kommerziell – Weitergabe unter gleichen Bedingungen 4.0 International Lizenz (CC BY-NC-SA 4.0) lizensiert: https://creativecommons.org/licenses/by-nc-sa/4.0/deed.de This document is licensed under a Creative Commons Attribution- NonCommercial-ShareAlike 4.0 International License (CC BY-NC-SA 4.0): https://creativecommons.org/licenses/by-nc-sa/4.0/deed.en Abstract Tackling the challenges of developing therapies for cancer, infections, or immune system disorders requires understanding and manipulation of the metabolic pathways related to a disease state or a pathogen. An essential role in the corresponding biochemical cascades is played by proteins of various types, whose functions are manifested through their interactions with other molecules. In basic and applied research, the functionality and binding properties of proteins are more often studied using peptide microarrays, which are collections of protein fragments displayed on a solid support in a spot array format. Commercially available peptide microarrays are manufactured using various methods, which result in different spot densities and costs per spot. While being relatively simple and straightforward to implement, the wide-spread SPOT- technique provides less than a thousand of peptides on a standard size substrate, which is not sufficient for many biological applications.
    [Show full text]
  • Longitudinal Development of Antibody Responses in COVID-19 Patients of Different Severity with ELISA, Peptide, and Glycan Arrays: an Immunological Case Series
    pathogens Article Longitudinal Development of Antibody Responses in COVID-19 Patients of Different Severity with ELISA, Peptide, and Glycan Arrays: An Immunological Case Series Jasmin Heidepriem 1,†, Christine Dahlke 2,3,4,*,† , Robin Kobbe 2, René Santer 5, Till Koch 2,3,4 , Anahita Fathi 2,3,4 , Bruna M. S. Seco 1 , My L. Ly 2,3,4, Stefan Schmiedel 2, Dorothee Schwinge 6, Sonia Serna 7 , Katrin Sellrie 1, Niels-Christian Reichardt 7,8, Peter H. Seeberger 1 , Marylyn M. Addo 2,3,4,* , Felix F. Loeffler 1,* and on behalf of the ID-UKE COVID-19 Study Group ‡ 1 Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Muehlenberg 1, 14476 Potsdam, Germany; [email protected] (J.H.); [email protected] (B.M.S.S.); [email protected] (K.S.); [email protected] (P.H.S.) 2 Division of Infectious Diseases, First Department of Medicine, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany; [email protected] (R.K.); [email protected] (T.K.); [email protected] (A.F.); [email protected] (M.L.L.); [email protected] (S.S.) 3 Department of Clinical Immunology of Infectious Diseases, Bernhard Nocht Institute for Tropical Medicine, 20251 Hamburg, Germany 4 German Center for Infection Research, Partner Site Hamburg-Lübeck-Borstel-Riems, Citation: Heidepriem, J.; Dahlke, C.; 20251 Hamburg, Germany 5 Kobbe, R.; Santer, R.; Koch, T.; Fathi, Department of Pediatrics, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany; A.; Seco, B.M.S.; Ly, M.L.; Schmiedel, [email protected] 6 I.
    [Show full text]
  • SARS-Cov-2 Epitope Mapping on Microarrays Highlights Strong Immune-Response to N Protein Region
    bioRxiv preprint doi: https://doi.org/10.1101/2020.11.09.374082; this version posted November 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. SARS-CoV-2 epitope mapping on microarrays highlights strong immune-response to N protein region Angelo Musicò§1, Roberto Frigerio§1, Alessandro Mussida1, Luisa Barzon2, Alessandro Sinigaglia2, Silvia Riccetti2, Federico Gobbi3, Chiara Piubelli3, Greta Bergamaschi1, Marcella Chiari1, Alessandro Gori#*, Marina Cretich#* 1: Consiglio Nazionale delle Ricerche, Istituto di Scienze e Tecnologie Chimiche “Giulio Natta”” (SCITEC), Via Mario Bianco 9, 20131, Milano, Italy 2: Department of Molecular Medicine, University of Padova, Via A. Gabelli 63, 35121 Padova, Italy 3: Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Via Don A. Sempreboni, 5 – 37024 Negrar di Valpolicella (Verona), Italy §: these authors equally contributed #: these authors equally contributed *: corresponding authors [email protected] ; [email protected] Abstract A workflow for SARS-CoV-2 epitope discovery on peptide microarrays is herein reported. The process started with a proteome-wide screening of immunoreactivity based on the use of a high-density microarray followed by a refinement and validation phase on a restricted panel of probes using microarrays with tailored peptide immobilization through a click-based strategy. Progressively larger, independent cohorts of Covid-19 positive sera were tested in the refinement processes, leading to the identification of immunodominant regions on SARS-CoV-2 Spike (S), Nucleocapsid (N) protein and Orf1ab polyprotein. A summary study testing 50 serum samples highlighted an epitope of the N protein (region 155-171) providing 92% sensitivity and 100% specificity of IgG detection in Covid-19 samples thus being a promising candidate for rapid implementation in serological tests.
    [Show full text]
  • And Light-Directed Peptide Microarray Synthesis
    Development of Chip-Based Electrochemically- and Light-Directed Peptide Microarray Synthesis by Pallav Kumar A Dissertation Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy Approved October 2013 by the Graduate Supervisory Committee: Neal Woodbury, Chair James Allen Stephen Johnston ARIZONA STATE UNIVERSITY December 2013 ABSTRACT Peptide microarrays may prove to be a powerful tool for proteomics research and clinical diagnosis applications. Fodor et al. and Maurer et al. have shown proof-of- concept methods of light- and electrochemically-directed peptide microarray fabrication on glass and semiconductor microchips respectively. In this work, peptide microarray fabrication based on the abovementioned techniques were optimized. In addition, MALDI mass spectrometry based peptide synthesis characterization on semiconductor microchips was developed and novel applications of a CombiMatrix (CBMX) platform for electrochemically controlled synthesis were explored. We have investigated performance of 2-(2-nitrophenyl)propoxycarbonyl (NPPOC) derivatives as photo-labile protecting group. Specifically, influence of substituents on 4 and 5 positions of phenyl ring of NPPOC group on the rate of photolysis and the yield of the amine was investigated. The results indicated that substituents capable of forming a π-network with the nitro group enhanced the rate of photolysis and yield. Once such properly substituted NPPOC groups were used, the rate of photolysis/yield depended on the nature of protected amino group indicating that a different chemical step during the photocleavage process became the rate limiting step. We also focused on electrochemically-directed parallel synthesis of high-density peptide microarrays using the CBMX technology referred to above which uses electrochemically generated acids to perform patterned chemistry.
    [Show full text]
  • Polypyrrole-Peptide Microarray for Biomolecular Interaction Analysis By
    Polypyrrole-peptide microarray for biomolecular interaction analysis by SPR imaging Marie-Bernadette Villiers 1 * , Sandra Cortès 1 , Carine Brakha 1 , Patrice Marche 1 , André Roget 2 , Thierry Livache 2 1 Institut d'oncologie/développement Albert Bonniot de Grenoble INSERM : U823 , CHU Grenoble , EFS , Université Joseph Fourier - Grenoble I , Institut Albert Bonniot, BP170, 38042 Grenoble Cedex 9,FR 2 SPRAM, Structures et propriétés d'architectures moléculaire CNRS : UMR5819 , Université Joseph Fourier - Grenoble I , CEA : DSM/INAC , 17 Rue des martyrs 38054 GRENOBLE CEDEX 9,FR * Correspondence should be adressed to: Marie-Bernadette Villiers <[email protected] > Abstract Nowadays, high-throughput analysis of biological events is a great challenge which could take benefit of the recent development of microarray devices. The great potential of such technology is related to the availability of a chip bearing a large set of probes, stable and easy to obtain, and suitable for ligand binding detection. Here, we described a new method based on polypyrrole chemistry and allowing the covalent immobilization of peptides in a microarray format and on a gold surface compatible with the use of Surface Plasmon Resonance. This technique is then illustrated by the detection and characterization of antibodies induced by hepatitis C virus and present in patients’serums. MESH Keywords Animals ; Antibodies ; analysis ; Humans ; Immunosorbent Techniques ; Models, Biological ; Peptides ; chemistry ; Polymers ; chemistry ; Protein Array Analysis ; instrumentation ; methods ; Protein Interaction Mapping ; instrumentation ; methods ; Pyrroles ; chemistry ; Surface Plasmon Resonance ; instrumentation ; methods Introduction Among the miniaturized devices used for biological analysis, chips bearing an array of peptides are of special interest. Indeed, peptide arrays open many applications such as enzyme substrate interaction measurement (1 ), epitope mapping (2 , 3 )… (for reviews, see (4 , 5 )).
    [Show full text]
  • Protein Microarrays As Tools for Functional Proteomics
    ics om & B te i ro o P in f f o o r l m a Journal of a n t r i Lueong et al., J Proteomics Bioinform 2013, S7 c u s o J DOI: 10.4172/jpb.S7-004 ISSN: 0974-276X Proteomics & Bioinformatics ReviewResearch Article Article OpenOpen Access Access Protein Microarrays as Tools for Functional Proteomics: Achievements, Promises and Challenges Smiths S Lueong, Jörg D Hoheisel and Mohamed Saiel Saeed Alhamdani* Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum (DKFZ), Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany Abstract The quest for a better understanding of organisms, and the human body in particular, at a comprehensive level has stimulated the development of techniques and processes that permit the analysis and assessment of biomedical information at high throughput. This has had substantial impact, not only by merely gathering knowledge but also by making scientists aware of the necessity to view organisms as complex biological systems rather than an assembly of individual biochemical pathways. Proteomics is still lagging behind genomics in this holistic analysis, however, because of the much higher degree of complexity that needs to be dealt with. Protein arrays, among other techniques, offer the prospect of advancing global protein analysis, similar to the impact of arrays in genomics. In basic research, protein arrays already contributed substantially, permitting the identification of many protein interactions and providing information on expression variations and structural changes, for example. Beside the fact of high throughput, arrays require relatively small reaction volumes, which is critical in view of the lack of means for in vitro protein amplification and beneficial for good assay sensitivity.
    [Show full text]
  • Quantitative Mapping of Protein-Peptide Affinity Landscapes
    RESEARCH ARTICLE Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads Huy Quoc Nguyen1‡, Jagoree Roy2†, Bjo¨ rn Harink1†, Nikhil P Damle2†§, Naomi R Latorraca3, Brian C Baxter4, Kara Brower5, Scott A Longwell5, Tanja Kortemme6,7, Kurt S Thorn4#, Martha S Cyert2, Polly Morrell Fordyce1,5,7,8* 1Department of Genetics, Stanford University, Stanford, United States; 2Department of Biology, Stanford University, Stanford, United States; 3Biophysics Program, Stanford University, Stanford, United States; 4Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United States; 5Department of Bioengineering, Stanford University, Stanford, United States; 6Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, United States; 7Chan Zuckerberg Biohub, San Francisco, United States; 8ChEM-H Institute, Stanford University, Stanford, United States *For correspondence: Abstract Transient, regulated binding of globular protein domains to Short Linear Motifs (SLiMs) [email protected] in disordered regions of other proteins drives cellular signaling. Mapping the energy landscapes of † These authors contributed these interactions is essential for deciphering and perturbing signaling networks but is challenging equally to this work due to their weak affinities. We present a powerful technology (MRBLE-pep) that simultaneously Present address: ‡Genentech, quantifies protein binding to a library of peptides directly synthesized
    [Show full text]
  • SPR Microscopy and Its Applications to High-Throughput Analyses of Biomolecular Binding Events and Their Kinetics
    ARTICLE IN PRESS Biomaterials 28 (2007) 2380–2392 www.elsevier.com/locate/biomaterials Review SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics Charles T. Campbella,Ã, Gibum Kimb aDepartment of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA bLumera Corporation, 19910 N, Creek Pkwy, suite 100, Bothell, WA 98011, USA Received 23 August 2006; accepted 31 January 2007 Abstract Surface plasmon resonance (SPR) sensing has long been used to study biomolecular binding events and their kinetics in a label-free way. This approach has recently been extended to SPR microscopy, which is an ideal tool for probing large microarrays of biomolecules for their binding interactions with various partners and the kinetics of such binding. Commercial SPR microscopes now make it possible to simultaneously monitor binding kinetics on 41300 spots within a protein microarray with a detection limit of 0.3 ng/cm2,oro50 fg per spot (o1 million protein molecules) with a time resolution of 1 s, and spot-to-spot reproducibility within a few percent. Such instruments should be capable of high-throughput kinetic studies of the binding of small (200 Da) ligands onto large protein microarrays. The method is label free and uses orders of magnitude less of the precious biomolecules than standard SPR sensing. It also gives the absolute bound amount and binding stoichiometry. r 2007 Elsevier Ltd. All rights reserved. Keywords: Surface plasmon resonance microscopy; Surface plasmon resonance imaging; Bioaffinity; Kinetics; Protein arrays; DNA arrays Contents 1. Introduction . 2380 2. Surface plasmon resonance (SPR) spectroscopy . 2382 3.
    [Show full text]
  • Antibody Validation with a Combination of Protein and Peptide Microarrays
    Application Note Antibody Validation with a Combination of Protein and Peptide Microarrays Introduction Antibodies are essential tools in life sciences and are applied in many research areas such as antibody-based proteomics, cellular biology or diagnostic test development. However, the specificity of mono- and polyclonal antibodies and hence their ability to generate reliable and robust data is under scrutiny in the scientific community. Non-specific binding or cross-reactions with non-target antigens may lead to inconclusive data or misleading results, and thus to a waste of time, money and samples. Highly specific antibodies are a prerequisite to generate reliable results, emphasizing the urgent need for appropriate validation methods in order to determine antibody specificity and cross-reactivity. Frequently used validation methods are often based on a purely functional approach (e.g. Western blot, IHC), but cover only a limited scope and - more importantly - lack a detailed understanding of antibody binding. This application note describes a novel experimental workflow for the unambiguous validation of antibodies by combining protein and peptide microarray screening (Fig. 1). In contrast to functional validation methods, this approach provides an in-depth view on antibody binding from the protein down to the epitope and amino acid level. Figure 1: Antibody validation workflow. Selected antibodies are initially screened with a HuProt® Human Proteome Microarray v3.1 covering ~20,000 full-length human proteins. Positive hit proteins are selected and printed as overlapping peptides on a PEPperCHIP® Peptide Microarray to perform a multiplexed epitope mapping with the same set of antibodies. In- depth analysis of the identified epitope(s) with regard to essential, conserved and variable amino acid positions by a PEPperMAP® Epitope Substitution Scan complete the antibody validation process.
    [Show full text]
  • SARS-Cov-2 Proteome-Wide Analysis Revealed Significant Epitope
    ORIGINAL RESEARCH published: 23 March 2021 doi: 10.3389/fimmu.2021.629185 SARS-CoV-2 Proteome-Wide Analysis Revealed Significant Epitope Signatures in COVID-19 Patients † † Tatjana Schwarz 1 , Kirsten Heiss 2 , Yuvaraj Mahendran 2, Fiordiligie Casilag 2, Florian Kurth 3, Leif E. Sander 3, Clemens-Martin Wendtner 4, Manuela A. Hoechstetter 4, ‡ Marcel A. Müller 1, Renate Sekul 2, Christian Drosten 1,5, Volker Stadler 2* ‡ and Victor M. Corman 1,5* 1 Institute of Virology, Charite´ —Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Edited by: zu Berlin, and Berlin Institute of Health, Berlin, Germany, 2 PEPperPRINT GmbH, R&D unit, Heidelberg, Germany, Sean X. Leng, 3 Department of Infectious Diseases and Respiratory Medicine, Charite´ —Universitätsmedizin Berlin, Corporate Member of Johns Hopkins University, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany, 4 Munich Clinic United States Schwabing, Academic Teaching Hospital, Ludwig-Maximilians University (LMU), Munich, Germany, 5 German Centre for Reviewed by: Infection Research, Associated Partner Charité, Berlin, Germany Andreas Radbruch, German Rheumatism Research Center (DRFZ), Germany The WHO declared the COVID-19 outbreak a public health emergency of international Hiromi Kubagawa, concern. The causative agent of this acute respiratory disease is a newly emerged German Rheumatism Research Center (DRFZ), Germany coronavirus, named SARS-CoV-2, which originated in China in late 2019. Exposure to *Correspondence: SARS−CoV−2 leads to multifaceted disease outcomes from asymptomatic infection to Victor M. Corman severe pneumonia, acute respiratory distress and potentially death. Understanding the [email protected] host immune response is crucial for the development of interventional strategies.
    [Show full text]
  • Pepperprint Epitope Mapping Solutions
    A Disruptive New Technology for Epitope Mapping! Only PEPperPRINT’s unique peptide microarray platform enables you to identify and map epitopes from antibodies and sera with unrivalled epitope resolution from single to hundreds of antigens at once order custom microarrays with up to 30,000 high fidelity peptides reliably receive your custom peptide microarrays within 3-5 weeks adjust your microarray from chip to chip and obtain rapid readouts benefit from the highest signal-to-noise ratios available cover protein conformations and posttranslational modifications Here are some examples of PEPperPRINT’s epitope mapping microarray applications: Linear and conformational epitope mappings for therapeutic antibodies in numerous development projects of various top 10 pharma companies Antibody cross-reactivity analyses for biopharmaceutical discovery projects Validation of diagnostic antibodies for an international diagnostics company In-depth specificity analysis of various anti-HLA-E research antibodies What our customers say: "Besides the fact that the data is useful to us, it remains as a fact that the quality of the testing is outstanding. The epitope is mapped." Patrizio Giacomini, Ph.D., (Assisstant Professor) Regina Elena National Cancer Institute, Rome "We have used the ‘Epitope Mapping Service’ from PEPperPRINT and the quality of results produced and professionalism of the service surpassed our expectations. The results obtained are very useful for our research and I would not hesitate to use this service in the future or to recommend it to fellow researchers working in the field." Javier Castillo-Olivares, Ph.D., (Fellow) Orbivirus Immunology, The Pirbright Institute, Woking “Congratulations to the whole PEPperPRINT team for the final report that is an example of clarity and good knowledge." Antonio Toniolo, M.D., (Professor of Medical Microbiology) FAMH, University of Insubria “The results are very clear and perfectly documented.
    [Show full text]