Datasheet M01464-1 Anti-PDIA3 Antibody

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Product datasheet Anti-PDIA3 Antibody Catalog Number: M01464-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-PDIA3 Antibody Gene Name PDIA3 Source Mouse Species Reactivity mouse,rat Tested Application IP, IF, IHC, WB Contents Antibody concentration: 1 mg/ml, stored in PBS pH7.4, 50% glycerol, 0.09% sodium azide Immunogen Human recombinant ERp57 (Grp58) Purification Protein G Purified Observed MW Dilution Ratios WB (1:2000), IHC (1:100), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user. Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme. It is mapped on 15q15.3. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. Reference Anti-PDIA3 Antibody被引用在0文献中。暂无引用 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-PDIA3 Antibody Catalog Number: M01464-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Selected Validation Data FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Powered by TCPDF (www.tcpdf.org).

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Processing and Presentation of Glycoproteins in the MHC Class I and II Antigen Presentation Pathways Denise Golgher, Tim Elliott and Mark Howarth
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PDIA3 Recombinant Protein Description Product Info
9853 Pacific Heights Blvd. Suite D. San Diego, CA 92121, USA Tel: 858-263-4982 Email: [email protected] 32-2652: PDIA3 Recombinant Protein ERp57,ERp60,ERp61,GRP57,GRP58,HsT17083,P58,PI-PLC,ER60,Protein disulfide-isomerase A3,Disulfide Alternative isomerase ER-60,Endoplasmic reticulum resident protein 60,ER protein 60,58 kDa microsomal Name : protein,Endoplasmic reticulum resident protein 57, Description Source : Escherichia Coli. PDIA3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 518 amino acids (25-505 a.a.) and having a molecular wieght of 58.5 kDa. The PDIA3 is fused to 37 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques. PDIA3 is an enzyme that belongs to the endoplasmic reticulum and interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. PDIA3 has protein disulfide isomerase activity. Complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. PDIA3 is expressed in the lumbar spinal cord from rats submitted to peripheral lesion during neonatal period. PDIA3 interacts with thiazide-sensitive sodium-chloride cotransporter in the kidney and is induced by glucose deprivation. PDIA3 is part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is important for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Product Info Amount : 25 µg Purification : Greater than 95.0% as determined by SDS-PAGE. The PDIA3 protein solution contains 20mM Tris-HCl, pH-8, 1mM DTT, 0.1M NaCl and 10% Content : glycerol.
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