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Animal Serum Multitest Slides Buffers and Solutions

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Animal Serum Code Description Size Price £ G210 Calf (Aseptic Donor) 100ml 13.90 G250 Calf (Aseptic Donor) 500ml 38.60 G212 Calf (Newborn) - under 14 days old 100ml 5.65 G252 Calf (Newborn) - under 14 days old 500ml 22.30 G610 Horse (Natural Clot) 100ml 7.80 G650 Horse (Natural Clot) 500ml 31.90 Multitest Slides Code Wells Size (mm) 61100-00 1 8 61100-01 3 8 61100-04 3 14 61100-05 8 6 61100-09 10 8 61100-10 2 10 61100-11 10 5 61100-12 36 2 61100-14 10 6 61100-16 10 7 61100-18 4 14 61100-19 12 8 61100-24 1 18 61100-25 15 4 61100-26 14 5 61100-28 21 4 61100-29 12 6 61100-30 3 11 61100-33 12 3 61100-36 18 5 61100-50 30 3 61100-60 4 6 Buffers and Solutions Code Description Size Price £ HDS15/25 Sorensen's Buffer pH 6.5 (1 vial makes 5L) 25 vials 35.00 HDS20/25 Sorensen's Buffer pH 7.0-7.6 (1 vial makes 5L)* 25 vials 36.75 HDS25 PBS for Osmotic Fragility (makes 250ml) 25ml 2.65 HDS35 PBS pH 7.2 or 7.6 for Immunofluorescence (makes 2L) 100ml 3.40 Immunocytochemistry Substrate Tablets Code Description Pack Price £ HD4190 AEC Effervescent Buffered pH 5.1 5mg x 50 44.80 HD4170 DAB Effervescent Buffered pH 7.0 10mg x 50 63.10 HD4240 DAB Unbuffered 10mg x 50 55.20 HD4120 Fast Red Standard Unbuffered 2mg x 50 47.90 HD4360 Ureaperoxide 5.68mg x 50 65.80 Embedding and Mounting Media, Fixatives and Chemicals Code Description Size Price £ Size Price £ HC8503 Acetone 2L 4.25 HC8510 Acacia Powder 100g 5.35 HC8520 Aluminium Sulphate 500g 4.20 HC8530 Aniline Xylene 500ml 11.15 HC8540 Beeswax - White 100g 4.10 500g 14.30 HC8542 Berlese Fluid (gum chloral) 500ml 55.15 HC8550 Canada Balsam (filtered) 25g 12.00 HC8555 Canada Balsam (dried) 25g 13.45 HC8560 Canada Balsam (50% in xylene) 25ml 11.05 100ml 28.10 HC8562 Canada Balsam (75% in xylene) 25ml 13.50 100ml 34.50 HC8565 Carbol Xylene 500ml 13.45 HC8570 Carnoy Fixative 500ml 6.40 HC8802 Schaudinn Fixative 500ml 8.20 Embedding and Mounting Media, Fixatives and Chemicals Continued Code Description Size Price £ Size Price £ HC8585 Charcoal - Activated 500g 6.10 CC500 Citroclear 25L 106.65 HC8595 Cytological Fixative Spray 50ml 2.30 HC8620 Di-n-butyl Phthalate 500ml 8.35 HC8625 Di Potassium Hydrogen Orthophosphate 500g 9.25 1kg 15.40 HC8630 Egg Albumen 25g 3.55 100g 10.50 HC8640 Farrant Medium 100ml 5.75 500ml 15.05 HC8645 Formaldehyde 40% 2.5L 10.00 25L 33.10 HC8650 Formaldehyde Neut. Buff. 10% 25L 20.75 HC8660 Formaldehyde - Saline 25L 20.75 HC8665 Formaldehyde - Saline 5 x conc. 5L 13.55 HC8675 Gelatin 100g 3.60 500g 10.65 HC8685 Glycerol 500ml 6.45 HC8690 Glycerin Albumen 25ml 2.55 100ml 6.55 HC8695 Glycerin Jelly 500g 12.25 HC8710 Gum Arabic 100ml 4.90 500ml 15.05 HC8717 Indian Ink 25ml 7.05 HC8720 Iodine (resublimed) 100g 7.80 500g 32.50 HC3951 Lactophenol 100ml 5.50 HC8725 Methanol (methyl alcohol) 25L 37.90 HC8735 Oil Cedarwood (natural) 500ml 21.30 HC8740 Oil Cedarwood (thickened for immersion) 500ml 29.50 HC8742 Oil Cedarwood (thickened - tropical grade) 100ml 9.15 HC8745 Oil Clove 100ml 7.80 HC8765 Paraffin Wax - MP 54°C 5Kg 17.40 10x1kg 47.47 HC8770 Paraffin Wax - MP 56°C 5Kg 17.40 10x1kg 47.47 HC8775 Paraffin Wax - MP 58°C 5Kg 17.40 10x1kg 47.47 HC8777 Paraffin Wax - Plasticised 56-58°C 10x1kg 53.20 HS640 Periodic Acid (1% aqueous) 250ml 6.55 HC8786 Polyvinyl Lactophenol 100ml 10.40 500ml 31.50 HC8787 Potassium Dihydrogen Orthophosphate 500g 6.70 1kg 11.40 HC8790 Potassium Iodide 500g 21.50 HC8802 Schaudinn Fixative 1L 13.45 HS735 Scott Tap Water Substitute 10 x conc. 100ml 6.00 HC8810 Susa Fixative 500ml 7.95 HC8838 Thiomersal (merthiolate) 25g 67.05 100g 170.95 HC8840 UV Free Mountant (aqueous) 100ml 9.80 HC8860 Zenker Fixative 500ml 6.10 Dry Dyes and Stains Code Description CI No. 10g £ 25g £ 100g £ HD1010 Acridine Orange 46005 7.55 37.05 HD1025 Acriflavine 46000 11.55 22.65 HD1045 Alcian Green 2GX 81.15 195.70 HD1050 Alcian Yellow 12840 153.00 HD1055 Alizarin Red S 58005 7.65 15.60 HD1060 Alizarin Yellow B 14030 6.90 13.90 34.65 HD1065 Alizarin Cyanin Green 61570 6.70 13.50 33.80 HD1070 Alkali Blue 5B 42750 10.10 19.85 HD1075 Alkali Blue 6B 42765 9.90 19.85 HD1080 Alphazurin A 42080 4.25 8.45 21.10 HD1085 Amaranth 16185 2.20 4.50 11.20 HD1092 Aniline Blue Alcohol Soluble 42755 23.10 HD1095 Aniline Blue/Orange G/PT Acid 11.70 23.35 HD1100 Astrazone Pink FG 48015 6.50 12.60 31.65 HD1105 Astrazone Red 6B 48020 6.50 12.60 31.65 HD1110 Atebrin 10.60 20.85 HD1115 Auramine O 41000 3.15 HD1120 Azo Black 30235 14.50 HD1130 Azo Phloxine 18050 6.40 13.00 32.50 HD1135 Azorubin S 16185 4.25 8.45 21.10 HD1140 Azur I 31.55 HD1145 Azur II 31.55 HD1150 Azur II Eosin 6.45 12.85 31.55 HD1155 Azur A 52005 18.65 37.35 Dry Dyes and Stains Continued Code Description CI No. 10g £ 25g £ 100g £ HD1160 Azur B 52010 33.50 65.80 HD1165 Azur A Eosinate 9.90 19.35 48.50 HD1170 Azur B Eosinate 10.70 20.90 52.40 HD1175 Biebrich Scarlet 26905 6.50 HD1180 Bismark Brown R 21010 3.10 6.25 15.35 HD1185 Bismark Brown Y 21000 4.25 8.70 21.30 HD1200 Brilliant Green 42040 2.30 4.60 11.85 HD1210 Brilliant Yellow 24890 4.90 9.90 HD1213 Bromocresol Green 14.20 27.75 HD1215 Carbol Fuchsin (powder) 6.30 12.60 HD1220 Carmalum (compound) 10.95 21.95 HD1225 Carmine 75470 18.55 36.80 HD1227 Carminic Acid 75470 99.20 HD1230 Carmoisine A 14720 35.00 HD1240 Celestine Blue 51050 57.60 HD1245 Chlorantine Fast Red 28160 54.60 HD1255 Chromotrope 2R 16570 7.25 HD1260 Chrysoidin Y 11270 5.00 7.60 19.90 HD1270 Congo Red 22120 7.65 HD1275 Coomassie Brilliant Blue G250 42655 4.75 9.70 23.80 HD1280 Coomassie Brilliant Blue R250 42660 4.75 9.70 24.30 HD1285 Cresyl Fast Violet 45.60 HD1290 Crystal Ponceau 16250 20.50 50.40 HD1315 Eosin Ethyl 45386 8.60 17.35 42.25 HD1326 Eriochrome Black T 14645 4.00 8.15 19.95 HD1327 Eriochrome Cyanin R 43810 6.55 13.00 HD1330 Erioglaucine 42090 4.95 9.90 24.30 HD1335 Erythrosin B 45430 7.10 14.20 20.10 HD1340 Ethyl Orange 6.95 13.90 HD1348 Ethyl Violet 42600 6.75 9.55 33.10 HD1355 Evans Blue 23860 75.25 HD1365 Fast Blue BB Salt 37175 6.75 13.20 HD1370 Fast Blue RR Salt 37155 19.90 38.90 HD1390 Fast Red 7B 26050 5.90 18.90 HD1405 Fast Violet B Salt 37165 81.90 HD1420 Fluorescein (free acid) 45350 2.30 4.70 11.85 HD1435 Fuchsin Basic for ZN 42510 4.25 HD1450 Gallocyanin 51030 14.10 27.55 68.95 HD1455 Giemsa Stain 5.30 HD1460 Gram Iodine (compound) 2.85 HD1465 Haemalum Mayer (compound) 75290 10.45 20.90 52.20 HD1470 Haematein 75290 34.95 69.90 HD1475 Haematoxylin 75290 27.35 HD1490 Haematoxylin (Harris) 52.25 HD1497 Indigo 73000 7.10 13.90 HD1500 Indigo Carmine 73015 7.20 13.15 42.20 HD1510 Janus Green B 11050 50.40 HD1515 Jenner Stain 4.20 HD1520 Lacmoid 51400 8.05 16.10 HD1545 Lissamine Fast Red B 17045 3.60 7.45 18.25 HD1550 Lissamine Fast Yellow 18965 4.85 9.90 24.30 HD1555 Lissamine Green B 44090 4.95 9.90 24.30 HD1565 Litmus (pH indicator) 14.20 27.75 HD1570 Lugol Iodine (compound) 3.70 HD1575 Luxol Fast Blue MBS 74180 3.00 HD1590 Malachite Green 42000 4.60 HD1595 Martius Yellow 10315 10.10 HD1605 May Grunwald Stain 5.45 HD1615 Metanil Yellow 13065 2.50 5.35 13.00 HD1620 Methyl Blue 42780 3.15 15.65 HD1625 Methyl Green 42585 8.05 16.05 39.40 HD1635 Methyl Orange 13025 2.90 5.75 14.20 HD1640 Methyl Red 13020 4.00 8.15 HD1645 Methyl Violet 2B 42535 4.60 7.90 19.45 HD1650 Methyl Violet 6B 42555 21.10 HD1653 Methyl Yellow 11020 3.20 6.55 15.90 HD1655 Methylene Blue (chloride) 52015 3.50 7.20 18.00 HD1660 Methylene Blue Alkaline (Loeffler) 5.30 HD1665 Methylene Blue (Unna) 5.30 26.35 Dry Dyes and Stains Continued Code Description CI No. 10g £ 25g £ 100g £ HD1670 Milling Yellow 18950 5.60 11.50 28.15 HD1680 Naphthalene Black 10B 20470 28.95 HD1685 Naphthol Green B 10020 3.45 7.10 17.75 HD1715 Neutral Red 50040 37.75 HD1718 New Coccine 16255 8.85 17.90 HD1720 New Fuchsin 42520 5.40 10.75 26.35 HD1725 New Methylene Blue 52030 43.80 168.40 HD1730 Nigrosin (alcohol soluble) 50415 2.70 5.55 13.85 HD1735 Nigrosin (water soluble) 50420 2.70 5.55 13.85 HD1742 Ninhydrin 7.80 15.65 38.35 HD1745 Nuclear Fast Red 60760 40.85 HD1750 Oil Red O 26125 4.85 10.45 26.00 HD1755 Orange G 16230 2.85 HD1778 Phenolphthalein 2.40 4.95 HD1780 Phloxine B 45410 6.65 13.20 32.40 HD1785 Ponceau Fuchsin (Masson) 22.45 HD1790 Ponceau 2G 16100 5.55 11.05 27.65 HD1795 Ponceau S 27195 9.05 18.10 HD1800 Ponceau de Xylidine 16150 3.60 HD1805 Pontamine Sky Blue 6BX 24410 4.20 HD1810 Pyronin B 45010 32.55 HD1818 Red FB 6.55 13.05 31.90 HD1820 Rhodamine B 45170 2.50 5.20 13.00 HD1825 Rhodamine 6G 45160 4.25 8.60 HD1830 Rosaniline HCl 42510 4.25 9.15 HD1835 Rose Bengal 45440 6.65 13.40 42.20 HD1840 Rosolic Acid 43800 10.75 21.50 HD1855 Scarlet R 26105 2.95 5.90 14.80 HD1870 Sirius Red F3B 35780 8.80 17.60 43.05 HD1877 Solochrome Cyanin R 43820 6.55 13.00 HD1880 Solochrome Black 14645 4.00 8.15 19.95 HD1890 Sudan II 12140 3.45 6.95 17.40 HD1895 Sudan III 26100 3.70 7.55 18.90 HD1900 Sudan IV 26105 2.95 5.90 14.50 HD1905 Sudan Black B 26150 4.55 22.90 HD1910 Sudan Yellow 12055 4.25 8.60 HD1915 Sulpho Naphthyl Red 3.25 6.65 16.20 HD1925 Sunset Yellow 15985 3.65 7.55 18.90 HD1930 Tartrazine 19140 2.15 4.30 10.60 HD1945 Thioflavine T 49005 19.20 38.25 HD1955 Titan Yellow 19540 7.45 14.90 HD1965 Tropaeolin O 14270 7.15 14.30 HD1970 Trypan Blue 23850 31.10 HD1980 Uranin 45350 3.10 6.15 15.35 HD1990 Victoria Blue B 44045 3.25 6.55 16.20 HD2000 Water Blue 6B Extra P 42780 6.45 12.85 HD2005 Wright's Stain 4.40 HD2010 Xylene Cyanol 43535 68.10 Wet Dyes and Stains Code Description 100ml £ 250ml £ 500ml £ 1L £ HS105 Albert Stain 1 7.70 HS110 Albert Stain 2 6.55 HS115 Alcian Blue 8GX 9.15 HS120 Aniline Blue (1% aqueous) 3.60 5.35 10.00 HS125 Aniline Blue (Masson)
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    457 Observations on a highly specific method for the histochemical detection of sulphated mucopolysaccharides, and its possible mechanisms By I. D. HEATH (From the Department of Anatomy, University of St. Andrews, Queen's College, Dundee. Present address: General Hospital, Nottingham) With 3 plates (figs, i to 3) Summary Whereas basic dyes in aqueous solutions stain chromatin, all mucins, mast cells, the ground substance of cartilage, and epidermis, it has been shown that a 0-03 % solution of basic dye in 5% aluminium sulphate produces a highly specific staining reaction for sulphated mucopolysaccharides. The best dyes are nuclear fast red (Herzberg) and methylene blue. Acid dyes in solutions of aluminium salts are induced to stain the ground substance of cartilage. These observations have been confirmed in a num- ber of species. Other metallic ions have similar properties and the use of green and purple chromic salts indicate that co-ordination plays a part in the reaction. Methylation, saponification, and sulphation experiments show that the sulphate group is essential. This has been confirmed by using pure chemical substances in gelatin models. Oxidation of keratin with performic acid, which produces sulphonic groups, causes hair (previously negative) to react. From this it is suggested that sul- phonic groups may also react, and that the reactive groups need not be attached to mucopolysaccharides. It is further suggested that the specificity of sulphated muco- polysaccharides is due to the fact that they are the only substances present in the tis- sues with a sufficient concentration of sulphate groups. Experiments with solochrome azurine show that the aluminium is attached to all tissue elements irrespective of their nature.
  • Biological Stains & Dyes

    Biological Stains & Dyes

    BIOLOGICAL STAINS & DYES Developed for Biology, microbiology & industrial applications ACRIFLAVIN ALCIAN BLUE 8GX ACRIDINE ORANGE ALIZARINE CYANINE GREEN ANILINE BLUE (SPIRIT SOLUBLE) www.lobachemie.com BIOLOGICAL STAINS & DYES Staining is an important technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues. Loba Chemie offers comprehensive range of Stains and dyes, which are frequently used in Microbiology, Hematology, Histology, Cytology, Protein and DNA Staining after Electrophoresis and Fluorescence Microscopy etc. Many of our stains and dyes have specifications complying certified grade of Biological Stain Commission, and suitable for biological research. Stringent testing on all batches is performed to ensure consistency and satisfy necessary specification particularly in challenging work such as histology and molecular biology. Stains and dyes offer by Loba chemie includes Dry – powder form Stains and dyes as well as wet - ready to use solutions. Features: • Ideally suited to molecular biology or microbiology applications • Available in a wide range of innovative chemical packaging options. Range of Biological Stains & Dyes Product Code Product Name C.I. No CAS No 00590 ACRIDINE ORANGE 46005 10127-02-3 00600 ACRIFLAVIN 46000 8063-24-9 00830 ALCIAN BLUE 8GX 74240 33864-99-2 00840 ALIZARINE AR 58000 72-48-0 00852 ALIZARINE CYANINE GREEN 61570 4403-90-1 00980 AMARANTH 16185 915-67-3 01010 AMIDO BLACK 10B 20470
  • Mechanistic Insights Into Photodegradation of Organic Dyes Using Heterostructure Photocatalysts

    Mechanistic Insights Into Photodegradation of Organic Dyes Using Heterostructure Photocatalysts

    catalysts Review Mechanistic Insights into Photodegradation of Organic Dyes Using Heterostructure Photocatalysts Yi-Hsuan Chiu 1 , Tso-Fu Mark Chang 2,*, Chun-Yi Chen 2,*, Masato Sone 2 and Yung-Jung Hsu 1,3,* 1 Department of Materials Science and Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan; [email protected] 2 Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503, Japan; [email protected] 3 Center for Emergent Functional Matter Science, National Chiao Tung University, Hsinchu 30010, Taiwan * Correspondence: [email protected] (T.-F.M.C.); [email protected] (C.-Y.C.); [email protected] (Y.-J.H.) Received: 12 April 2019; Accepted: 3 May 2019; Published: 9 May 2019 Abstract: Due to its low cost, environmentally friendly process, and lack of secondary contamination, the photodegradation of dyes is regarded as a promising technology for industrial wastewater treatment. This technology demonstrates the light-enhanced generation of charge carriers and reactive radicals that non-selectively degrade various organic dyes into water, CO2, and other organic compounds via direct photodegradation or a sensitization-mediated degradation process. The overall efficiency of the photocatalysis system is closely dependent upon operational parameters that govern the adsorption and photodegradation of dye molecules, including the initial dye concentration, pH of the solution, temperature of the reaction medium, and light intensity.Additionally, the charge-carrier properties of the photocatalyst strongly affect the generation of reactive species in the heterogeneous photodegradation and thereby dictate the photodegradation efficiency.
  • Color Regulatory Affairs Quality Standards for Colors a Safety

    Color Regulatory Affairs Quality Standards for Colors a Safety

    Color Regulatory Affairs Quality Standards for Colors A safety perspective for the brands Sue Ann McAvoy, MS, CFS Sensient Colors LLC September 23, 2015 Why do we use colorants? Effective Color Usage Drives Consumer Trial and Acceptance • …a lasting color impression is made within 90 seconds and accounts for 60 percent of the acceptance or rejection of an object … • Color has a major impact on flavor perception and flavor acceptance. • Color plays a critical role in product identification. Research has indicated that people “see” flavors (Acree 2013, Lewis and others 2008), i.e, they form an opinion about the flavor of a food or beverage, based on its color. What is Permitted Worldwide? Issues Facing the Global Color Industry .Southampton Study .Sustainability Image can be enhanced by renewable natural colors .Ongoing food safety issues .Lack of specific quality and safety specifications in the regulations .On going import detentions involving color Synthetic Raw Materials Under Pressure • Increasing concern and Central Government activity around chemical industry in China and India • Availability of raw materials has become much more volatile • Cost are increasing and needs for environmental controls become more wide spread • Cost could double for synthetic colors over next 2 – 3 years • Natural colors may become an attractive alternatives from a supply security and environmentally responsible standpoint. Trends Towards Color from Naturals Sources in Europe CONFIDENTIAL Global Food Colours Market by Country/Region, 2012 17% 9% 36% Europe