Animal Serum Multitest Slides Buffers and Solutions

Total Page:16

File Type:pdf, Size:1020Kb

Animal Serum Multitest Slides Buffers and Solutions Animal Serum Code Description Size Price £ G210 Calf (Aseptic Donor) 100ml 13.90 G250 Calf (Aseptic Donor) 500ml 38.60 G212 Calf (Newborn) - under 14 days old 100ml 5.65 G252 Calf (Newborn) - under 14 days old 500ml 22.30 G610 Horse (Natural Clot) 100ml 7.80 G650 Horse (Natural Clot) 500ml 31.90 Multitest Slides Code Wells Size (mm) 61100-00 1 8 61100-01 3 8 61100-04 3 14 61100-05 8 6 61100-09 10 8 61100-10 2 10 61100-11 10 5 61100-12 36 2 61100-14 10 6 61100-16 10 7 61100-18 4 14 61100-19 12 8 61100-24 1 18 61100-25 15 4 61100-26 14 5 61100-28 21 4 61100-29 12 6 61100-30 3 11 61100-33 12 3 61100-36 18 5 61100-50 30 3 61100-60 4 6 Buffers and Solutions Code Description Size Price £ HDS15/25 Sorensen's Buffer pH 6.5 (1 vial makes 5L) 25 vials 35.00 HDS20/25 Sorensen's Buffer pH 7.0-7.6 (1 vial makes 5L)* 25 vials 36.75 HDS25 PBS for Osmotic Fragility (makes 250ml) 25ml 2.65 HDS35 PBS pH 7.2 or 7.6 for Immunofluorescence (makes 2L) 100ml 3.40 Immunocytochemistry Substrate Tablets Code Description Pack Price £ HD4190 AEC Effervescent Buffered pH 5.1 5mg x 50 44.80 HD4170 DAB Effervescent Buffered pH 7.0 10mg x 50 63.10 HD4240 DAB Unbuffered 10mg x 50 55.20 HD4120 Fast Red Standard Unbuffered 2mg x 50 47.90 HD4360 Ureaperoxide 5.68mg x 50 65.80 Embedding and Mounting Media, Fixatives and Chemicals Code Description Size Price £ Size Price £ HC8503 Acetone 2L 4.25 HC8510 Acacia Powder 100g 5.35 HC8520 Aluminium Sulphate 500g 4.20 HC8530 Aniline Xylene 500ml 11.15 HC8540 Beeswax - White 100g 4.10 500g 14.30 HC8542 Berlese Fluid (gum chloral) 500ml 55.15 HC8550 Canada Balsam (filtered) 25g 12.00 HC8555 Canada Balsam (dried) 25g 13.45 HC8560 Canada Balsam (50% in xylene) 25ml 11.05 100ml 28.10 HC8562 Canada Balsam (75% in xylene) 25ml 13.50 100ml 34.50 HC8565 Carbol Xylene 500ml 13.45 HC8570 Carnoy Fixative 500ml 6.40 HC8802 Schaudinn Fixative 500ml 8.20 Embedding and Mounting Media, Fixatives and Chemicals Continued Code Description Size Price £ Size Price £ HC8585 Charcoal - Activated 500g 6.10 CC500 Citroclear 25L 106.65 HC8595 Cytological Fixative Spray 50ml 2.30 HC8620 Di-n-butyl Phthalate 500ml 8.35 HC8625 Di Potassium Hydrogen Orthophosphate 500g 9.25 1kg 15.40 HC8630 Egg Albumen 25g 3.55 100g 10.50 HC8640 Farrant Medium 100ml 5.75 500ml 15.05 HC8645 Formaldehyde 40% 2.5L 10.00 25L 33.10 HC8650 Formaldehyde Neut. Buff. 10% 25L 20.75 HC8660 Formaldehyde - Saline 25L 20.75 HC8665 Formaldehyde - Saline 5 x conc. 5L 13.55 HC8675 Gelatin 100g 3.60 500g 10.65 HC8685 Glycerol 500ml 6.45 HC8690 Glycerin Albumen 25ml 2.55 100ml 6.55 HC8695 Glycerin Jelly 500g 12.25 HC8710 Gum Arabic 100ml 4.90 500ml 15.05 HC8717 Indian Ink 25ml 7.05 HC8720 Iodine (resublimed) 100g 7.80 500g 32.50 HC3951 Lactophenol 100ml 5.50 HC8725 Methanol (methyl alcohol) 25L 37.90 HC8735 Oil Cedarwood (natural) 500ml 21.30 HC8740 Oil Cedarwood (thickened for immersion) 500ml 29.50 HC8742 Oil Cedarwood (thickened - tropical grade) 100ml 9.15 HC8745 Oil Clove 100ml 7.80 HC8765 Paraffin Wax - MP 54°C 5Kg 17.40 10x1kg 47.47 HC8770 Paraffin Wax - MP 56°C 5Kg 17.40 10x1kg 47.47 HC8775 Paraffin Wax - MP 58°C 5Kg 17.40 10x1kg 47.47 HC8777 Paraffin Wax - Plasticised 56-58°C 10x1kg 53.20 HS640 Periodic Acid (1% aqueous) 250ml 6.55 HC8786 Polyvinyl Lactophenol 100ml 10.40 500ml 31.50 HC8787 Potassium Dihydrogen Orthophosphate 500g 6.70 1kg 11.40 HC8790 Potassium Iodide 500g 21.50 HC8802 Schaudinn Fixative 1L 13.45 HS735 Scott Tap Water Substitute 10 x conc. 100ml 6.00 HC8810 Susa Fixative 500ml 7.95 HC8838 Thiomersal (merthiolate) 25g 67.05 100g 170.95 HC8840 UV Free Mountant (aqueous) 100ml 9.80 HC8860 Zenker Fixative 500ml 6.10 Dry Dyes and Stains Code Description CI No. 10g £ 25g £ 100g £ HD1010 Acridine Orange 46005 7.55 37.05 HD1025 Acriflavine 46000 11.55 22.65 HD1045 Alcian Green 2GX 81.15 195.70 HD1050 Alcian Yellow 12840 153.00 HD1055 Alizarin Red S 58005 7.65 15.60 HD1060 Alizarin Yellow B 14030 6.90 13.90 34.65 HD1065 Alizarin Cyanin Green 61570 6.70 13.50 33.80 HD1070 Alkali Blue 5B 42750 10.10 19.85 HD1075 Alkali Blue 6B 42765 9.90 19.85 HD1080 Alphazurin A 42080 4.25 8.45 21.10 HD1085 Amaranth 16185 2.20 4.50 11.20 HD1092 Aniline Blue Alcohol Soluble 42755 23.10 HD1095 Aniline Blue/Orange G/PT Acid 11.70 23.35 HD1100 Astrazone Pink FG 48015 6.50 12.60 31.65 HD1105 Astrazone Red 6B 48020 6.50 12.60 31.65 HD1110 Atebrin 10.60 20.85 HD1115 Auramine O 41000 3.15 HD1120 Azo Black 30235 14.50 HD1130 Azo Phloxine 18050 6.40 13.00 32.50 HD1135 Azorubin S 16185 4.25 8.45 21.10 HD1140 Azur I 31.55 HD1145 Azur II 31.55 HD1150 Azur II Eosin 6.45 12.85 31.55 HD1155 Azur A 52005 18.65 37.35 Dry Dyes and Stains Continued Code Description CI No. 10g £ 25g £ 100g £ HD1160 Azur B 52010 33.50 65.80 HD1165 Azur A Eosinate 9.90 19.35 48.50 HD1170 Azur B Eosinate 10.70 20.90 52.40 HD1175 Biebrich Scarlet 26905 6.50 HD1180 Bismark Brown R 21010 3.10 6.25 15.35 HD1185 Bismark Brown Y 21000 4.25 8.70 21.30 HD1200 Brilliant Green 42040 2.30 4.60 11.85 HD1210 Brilliant Yellow 24890 4.90 9.90 HD1213 Bromocresol Green 14.20 27.75 HD1215 Carbol Fuchsin (powder) 6.30 12.60 HD1220 Carmalum (compound) 10.95 21.95 HD1225 Carmine 75470 18.55 36.80 HD1227 Carminic Acid 75470 99.20 HD1230 Carmoisine A 14720 35.00 HD1240 Celestine Blue 51050 57.60 HD1245 Chlorantine Fast Red 28160 54.60 HD1255 Chromotrope 2R 16570 7.25 HD1260 Chrysoidin Y 11270 5.00 7.60 19.90 HD1270 Congo Red 22120 7.65 HD1275 Coomassie Brilliant Blue G250 42655 4.75 9.70 23.80 HD1280 Coomassie Brilliant Blue R250 42660 4.75 9.70 24.30 HD1285 Cresyl Fast Violet 45.60 HD1290 Crystal Ponceau 16250 20.50 50.40 HD1315 Eosin Ethyl 45386 8.60 17.35 42.25 HD1326 Eriochrome Black T 14645 4.00 8.15 19.95 HD1327 Eriochrome Cyanin R 43810 6.55 13.00 HD1330 Erioglaucine 42090 4.95 9.90 24.30 HD1335 Erythrosin B 45430 7.10 14.20 20.10 HD1340 Ethyl Orange 6.95 13.90 HD1348 Ethyl Violet 42600 6.75 9.55 33.10 HD1355 Evans Blue 23860 75.25 HD1365 Fast Blue BB Salt 37175 6.75 13.20 HD1370 Fast Blue RR Salt 37155 19.90 38.90 HD1390 Fast Red 7B 26050 5.90 18.90 HD1405 Fast Violet B Salt 37165 81.90 HD1420 Fluorescein (free acid) 45350 2.30 4.70 11.85 HD1435 Fuchsin Basic for ZN 42510 4.25 HD1450 Gallocyanin 51030 14.10 27.55 68.95 HD1455 Giemsa Stain 5.30 HD1460 Gram Iodine (compound) 2.85 HD1465 Haemalum Mayer (compound) 75290 10.45 20.90 52.20 HD1470 Haematein 75290 34.95 69.90 HD1475 Haematoxylin 75290 27.35 HD1490 Haematoxylin (Harris) 52.25 HD1497 Indigo 73000 7.10 13.90 HD1500 Indigo Carmine 73015 7.20 13.15 42.20 HD1510 Janus Green B 11050 50.40 HD1515 Jenner Stain 4.20 HD1520 Lacmoid 51400 8.05 16.10 HD1545 Lissamine Fast Red B 17045 3.60 7.45 18.25 HD1550 Lissamine Fast Yellow 18965 4.85 9.90 24.30 HD1555 Lissamine Green B 44090 4.95 9.90 24.30 HD1565 Litmus (pH indicator) 14.20 27.75 HD1570 Lugol Iodine (compound) 3.70 HD1575 Luxol Fast Blue MBS 74180 3.00 HD1590 Malachite Green 42000 4.60 HD1595 Martius Yellow 10315 10.10 HD1605 May Grunwald Stain 5.45 HD1615 Metanil Yellow 13065 2.50 5.35 13.00 HD1620 Methyl Blue 42780 3.15 15.65 HD1625 Methyl Green 42585 8.05 16.05 39.40 HD1635 Methyl Orange 13025 2.90 5.75 14.20 HD1640 Methyl Red 13020 4.00 8.15 HD1645 Methyl Violet 2B 42535 4.60 7.90 19.45 HD1650 Methyl Violet 6B 42555 21.10 HD1653 Methyl Yellow 11020 3.20 6.55 15.90 HD1655 Methylene Blue (chloride) 52015 3.50 7.20 18.00 HD1660 Methylene Blue Alkaline (Loeffler) 5.30 HD1665 Methylene Blue (Unna) 5.30 26.35 Dry Dyes and Stains Continued Code Description CI No. 10g £ 25g £ 100g £ HD1670 Milling Yellow 18950 5.60 11.50 28.15 HD1680 Naphthalene Black 10B 20470 28.95 HD1685 Naphthol Green B 10020 3.45 7.10 17.75 HD1715 Neutral Red 50040 37.75 HD1718 New Coccine 16255 8.85 17.90 HD1720 New Fuchsin 42520 5.40 10.75 26.35 HD1725 New Methylene Blue 52030 43.80 168.40 HD1730 Nigrosin (alcohol soluble) 50415 2.70 5.55 13.85 HD1735 Nigrosin (water soluble) 50420 2.70 5.55 13.85 HD1742 Ninhydrin 7.80 15.65 38.35 HD1745 Nuclear Fast Red 60760 40.85 HD1750 Oil Red O 26125 4.85 10.45 26.00 HD1755 Orange G 16230 2.85 HD1778 Phenolphthalein 2.40 4.95 HD1780 Phloxine B 45410 6.65 13.20 32.40 HD1785 Ponceau Fuchsin (Masson) 22.45 HD1790 Ponceau 2G 16100 5.55 11.05 27.65 HD1795 Ponceau S 27195 9.05 18.10 HD1800 Ponceau de Xylidine 16150 3.60 HD1805 Pontamine Sky Blue 6BX 24410 4.20 HD1810 Pyronin B 45010 32.55 HD1818 Red FB 6.55 13.05 31.90 HD1820 Rhodamine B 45170 2.50 5.20 13.00 HD1825 Rhodamine 6G 45160 4.25 8.60 HD1830 Rosaniline HCl 42510 4.25 9.15 HD1835 Rose Bengal 45440 6.65 13.40 42.20 HD1840 Rosolic Acid 43800 10.75 21.50 HD1855 Scarlet R 26105 2.95 5.90 14.80 HD1870 Sirius Red F3B 35780 8.80 17.60 43.05 HD1877 Solochrome Cyanin R 43820 6.55 13.00 HD1880 Solochrome Black 14645 4.00 8.15 19.95 HD1890 Sudan II 12140 3.45 6.95 17.40 HD1895 Sudan III 26100 3.70 7.55 18.90 HD1900 Sudan IV 26105 2.95 5.90 14.50 HD1905 Sudan Black B 26150 4.55 22.90 HD1910 Sudan Yellow 12055 4.25 8.60 HD1915 Sulpho Naphthyl Red 3.25 6.65 16.20 HD1925 Sunset Yellow 15985 3.65 7.55 18.90 HD1930 Tartrazine 19140 2.15 4.30 10.60 HD1945 Thioflavine T 49005 19.20 38.25 HD1955 Titan Yellow 19540 7.45 14.90 HD1965 Tropaeolin O 14270 7.15 14.30 HD1970 Trypan Blue 23850 31.10 HD1980 Uranin 45350 3.10 6.15 15.35 HD1990 Victoria Blue B 44045 3.25 6.55 16.20 HD2000 Water Blue 6B Extra P 42780 6.45 12.85 HD2005 Wright's Stain 4.40 HD2010 Xylene Cyanol 43535 68.10 Wet Dyes and Stains Code Description 100ml £ 250ml £ 500ml £ 1L £ HS105 Albert Stain 1 7.70 HS110 Albert Stain 2 6.55 HS115 Alcian Blue 8GX 9.15 HS120 Aniline Blue (1% aqueous) 3.60 5.35 10.00 HS125 Aniline Blue (Masson)
Recommended publications
  • Lysochrome Dyes Sudan Dyes, Oil Red Fat Soluble Dyes Used for Biochemical Staining of Triglycerides, Fatty Acids, and Lipoproteins Product Description
    FT-N13862 Lysochrome dyes Sudan dyes, Oil red Fat soluble dyes used for biochemical staining of triglycerides, fatty acids, and lipoproteins Product Description Name : Sudan IV Other names: Sudan R, C.I. Solvent Red 24, C.I. 26105, Lipid Crimson, Oil Red, Oil Red BB, Fat Red B, Oil Red IV, Scarlet Red, Scarlet Red N.F, Scarlet Red Scharlach, Scarlet R Catalog Number : N13862, 100g Structure : CAS: [85-83-6] Molecular Weight : MW: 380.45 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs =513-529nm(red);Sol(EtOH):0.09% S:22/23/24/25 Name : Sudan III Other names: Rouge Sudan ; rouge Ceresin ; CI 26100; CI Solvent Red 23 Catalog Number : 08002A, 25g Structure : CAS:[85-86-9] Molecular Weight : MW: 352.40 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs =503-510nm(red);Sol(EtOH):0.15% S:24/25 Name : Sudan Black B Other names: Sudan Black; Fat Black HB; Solvent Black 3; C.I. 26150 Catalog Number : 279042, 50g AR7910, 100tests stain for lipids granules Structure : CAS: [4197-25-5] S:22/23/24/25 Molecular Weight : MW: 456.54 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs=596-605nm(blue-black) Name : Oil Red O Other names: Solvent Red 27, Sudan Red 5B, C.I. 26125 Catalog Number : N13002, 100g Structure : CAS: [1320-06-5 ] Molecular Weight : MW: 408.51 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs =518(359)nm(red);Sol(EtOH): moderate; Sol(water): Insoluble S:22/23/24/25 Storage: Room temperature (Z) P.1 FT-N13862 Technical information & Directions for use A lysochrome is a fat soluble dye that have high affinity to fats, therefore are used for biochemical staining of triglycerides, fatty acids, and lipoproteins.
    [Show full text]
  • (12) United States Patent (10) Patent No.: US 6,905,539 B2 Patel Et Al
    USOO6905539B2 (12) United States Patent (10) Patent No.: US 6,905,539 B2 Patel et al. (45) Date of Patent: Jun. 14, 2005 (54) BLACK ERADICABLE INK, METHODS OF 5,478,382. A 12/1995 Miller et al............... 106/22 B ERADICATION OF THE SAME, 5,486.228 A 1/1996 Miller et al. ..... ... 106/22 B ERADICABLE INK KIT, AND ERADICATED 5,489,331 A 2/1996 Miller et al. .............. 106/22 B INK COMPLEX 5,492,558 A 2/1996 Miller et al. .............. 106/22 B 5,498.282 A 3/1996 Miller et al. .. ... 106/22 B (75) Inventors: Sanjay Patel, Cypress, CA (US); David 5,498,285 A 3/1996 Hooykaas ................... 106/486 Godbout, Westmont, IL (US); Wing 5,499,881. A 3/1996 Chang........................ 401/17 Sum Vincent Kwan, Chicago, IL (US) E. A to: Eli - - - t; (73) Assignee: Sanford L.P., Freeport, IL (US) SEA GE Air O..."; (*) Notice: Subject to any disclaimer, the term of this SCA : R. S. G.O.". patent is extended or adjusted under 35 5.877234. A 3/1999 Xuetal... 523/161 U.S.C. 154(b) by 0 days. 5,916,357. A 6/1999 Wang et al. ............. 106/31.23 5,964,931 A 10/1999 Korper .................... 106/31.93 (21) Appl. No.: 10/619,706 5,977.211 A 11/1999 Koyama ..................... 523/161 5.997,891 A 12/1999 Fuerst et al. ................ 424/401 (22) Filed: Jul. 15, 2003 6,037,391 A 3/2000 Iida ............................ 523/161 6,048.914 A 4/2000 Goto et al.
    [Show full text]
  • Study of the Effect of Sudan II and Treatment Water Coupled with Fe2+ in Modulating Hematological and Biochemical Changes in Rabbits’
    J Biochem Tech (2019) 10(1): 79-84 ISSN: 0974-2328 Study of the Effect of Sudan II and Treatment water Coupled with Fe2+ in Modulating Hematological and Biochemical Changes in Rabbits’ Elham M. Hussien*, Sawsan M. Abu El Hassan and Ferial M.N. Fudllalah Received: 19 November 2018 / Received in revised form: 02 March 2019, Accepted: 07 March 2019, Published online: 24 March 2019 © Biochemical Technology Society 2014-2019 © Sevas Educational Society 2008 Abstract Throughout the years, azo compounds including Sudan dyes have Finally, the present study proved that Sudan II treated with ferrous been widely used in industry as colorants in food, cosmetics, sulfate can keep hematological parameters, liver and kidney waxes, solvents, textiles, plastics and printing inks. Sudan dyes I, functions near the physiological normal state. II, III, IV, and their degradation products have been regarded to hurt human health. They cause cancer due to their teratogenicity, Keywords: Ferrous sulfate, Sudan dyes, liver and kidney genotoxicity and carcinogenicity effects. In the present work, the functions and rabbits. efficiency of ferrous sulfate with low cost and the first choice in the treatment of printing, dyeing and electroplating wastewater to Introduction reduce the toxicity of Sudan II-induced oxidative damage to hematological parameters, liver and kidney functions in adult Synthetic or natural food additives may be used as flavoring or female and male rabbits was investigated. Animals which received coloring tools (Sayed et al., 2012). Moreover, Amin et al. (2010) Sudan II were treated with ferrous sulfate as the sole source of explained that colorants provide an aesthetic appearance to foods potent liquid for 14 days at the dose of 111mg/kg B.wt before preferred by consumers.
    [Show full text]
  • Student Safety Sheets Dyes, Stains & Indicators
    Student safety sheets 70 Dyes, stains & indicators Substance Hazard Comment Solid dyes, stains & indicators including: DANGER: May include one or more of the following Acridine orange, Congo Red (Direct dye 28), Crystal violet statements: fatal/toxic if swallowed/in contact (methyl violet, Gentian Violet, Gram’s stain), Ethidium TOXIC HEALTH with skin/ if inhaled; causes severe skin burns & bromide, Malachite green (solvent green 1), Methyl eye damage/ serious eye damage; may cause orange, Nigrosin, Phenolphthalein, Rosaniline, Safranin allergy or asthma symptoms or breathing CORR. IRRIT. difficulties if inhaled; may cause genetic defects/ cancer/damage fertility or the unborn child; causes damages to organs/through prolonged or ENVIRONMENT repeated exposure. Solid dyes, stains & indicators including Alizarin (1,2- WARNING: May include one or more of the dihydroxyanthraquinone), Alizarin Red S, Aluminon (tri- following statements: harmful if swallowed/in ammonium aurine tricarboxylate), Aniline Blue (cotton / contact with skin/if inhaled; causes skin/serious spirit blue), Brilliant yellow, Cresol Red, DCPIP (2,6-dichl- eye irritation; may cause allergic skin reaction; orophenolindophenol, phenolindo-2,6-dichlorophenol, HEALTH suspected of causing genetic PIDCP), Direct Red 23, Disperse Yellow 7, Dithizone (di- defects/cancer/damaging fertility or the unborn phenylthiocarbazone), Eosin (Eosin Y), Eriochrome Black T child; may cause damage to organs/respiratory (Solochrome black), Fluorescein (& disodium salt), Haem- HARMFUL irritation/drowsiness or dizziness/damage to atoxylin, HHSNNA (Patton & Reeder’s indicator), Indigo, organs through prolonged or repeated exposure. Magenta (basic Fuchsin), May-Grunwald stain, Methyl- ene blue, Methyl green, Orcein, Phenol Red, Procion ENVIRON. dyes, Pyronin, Resazurin, Sudan I/II/IV dyes, Sudan black (Solvent Black 3), Thymol blue, Xylene cyanol FF Solid dyes, stains & indicators including Some dyes may contain hazardous impurities and Acid blue 40, Blue dextran, Bromocresol green, many have not been well researched.
    [Show full text]
  • The Sensitizing and Indicator Action of Victoria Blue and Janus Green on the Flocculation Reaction for Syphilis
    In the case of sulphonamides, cultures resistant to sulphanilamide were resistant or partially resistant to the other members of this group with the exception of marfanil. Resistance once acquired seems to be permanent, and so far we have not been successful in reducing it in vitro. REFERENCES. ALBERT, A., FRANCIS, A. E., GARROD, L. P., AND LINNELL, W. H.-(1938) Brit. J. exp. Path., 19, 41. LANDY, M., LARKUM, N. W., OswALD, E. J., AND STREIGHTOFF, F.-(1943) Science, 97, 265. LEVADITI, C., AND MCINTOSH, J.-(1910) Bull. Soc. Path. exot., 3, 368. MACLEAN, I. H., ROGERS, K. B., AND FLEMING, A.-(1939) Lancet, i, 562. MACLEOD, C. M.-(1940) J. exp. Med., 72, 217. RAMMELKAMP, C. H., AND MAXON, T.-(1942) Proc. Soc. exp. Biol., N.Y., 51, 386. RUBBO, S. D., ALBERT, A., AND MAxWELL, M.-(1942) Brit. J. exp. Path., 23, 69. TILLETT, W. S., CAMBIER, M. J., AND HARRIS, W. H.-(1943) J. clin. Invest., 22, 249. THE SENSITIZING AND INDICATOR ACTION OF VICTORIA BLUE AND JANUS GREEN ON THE FLOCCULATION REACTION FOR SYPHILIS. F. M. BERGER. From the Public Health Laboratory, County Hall, Wakefield. Received for publication November 9, 1943. DEAN (1937) found that isamine blue could act as an indicator of the reaction between an antigen and its homologous antibody. The addition of the dye to a mixture of horse serum and dilute antiserum produced a precipitate which was easily visible because it took up all the dye from the supernatant fluid. Prof. P. L. Suther- land suggested the possibility of using isamine blue as indicator in serological tests for syphilis.
    [Show full text]
  • Sudan Dyes and Other Illegal Dyes
    Sudan dyes and other illegal dyes Analysis in spices and food Sudan dyes, e.g. Sudan I to IV, Sudan In October 2004, the governmental Orange G, Sudan Red B, Sudan Red G, chemical institute “Bergisches Land” in Sudan Red 7B, and Sudan Black B as Wuppertal, Germany, reported the well as other dyes like 4-(Dimethylamino)- identification of two prohibited colours: azobenzene (Butter Yellow) and Para Butter Yellow and Para Red in bell pepper Red are basically synthetically produced powder and curry. azo dyes. Their degradation products are In February 2005, Great Britain experi- considered to be carcinogens and enced an extensive Rapid Alert action teratogens. Due to this fact, the EU does cycle. Due to the widespread use of one not permit the use of these colours as batch of chilli powder contaminated with food additives. However, in some Sudan I, batches of different products like countries, these dyes are still occasionally Worcester sauce, pizzas, pot noodles and used in order to intensify the colour of bell seafood sauces were subjected to com- pepper and chilli powder. plete recalls. Background Another recent concern is the discovery of the natural colour annatto in food and During 2003, the EU-Rapid Alert System spice mixes. Annatto (E 160b) is permit- (RASFF) issued a series of notifications ted by the EU for use in a variety of foods concerning the presence of Sudan dyes and beverages but not in spices and in chilli products and others such as spice mixtures. Its main colouring constit- spices, mixtures of spices, tomato uent is Bixin, with Norbixin being present sauces, pastas and sausages.
    [Show full text]
  • New Tetrachromic VOF Stain (Type III-G.S) for Normal and Pathological Fish Tissues C
    ORIGINAL PAPER New Tetrachromic VOF Stain (Type III-G.S) for Normal and Pathological Fish Tissues C. Sarasquete,* M. Gutiérrez Instituto de Ciencias Marinas de Andalucía, CSIC Polígono Río San Pedro, Apdo oficial, Puerto Real, Cádiz, Spain richrome methods invariably use dyes in acid ©2005, European Journal of Histochemistry pH solvents, usually diluted in aqueous acetic Tacid, and the concentration of this acid A new VOF Type III-G.S stain was applied to histological sec- matches the concentration of dye. Staining depends tions of different organs and tissues of healthy and pathologi- largely on the attachment of dyes to proteins. The cal larvae, juvenile and adult fish species (Solea senegalensis; acid pH itself is necessary to maximise the amount Sparus aurata; Diplodus sargo; Pagrus auriga; Argyrosomus regius and Halobatrachus didactylus). In comparison to the of dye that will attach to tissue amino groups. original Gutiérrez´VOF stain, more acid dyes of contrasting Proteins have both positively (amino groups) and colours and polychromatic/metachromatic properties were negatively (carboxyl and hydroxyl) charged groups. incorporated as essential constituents of the tetrachromic VOF Usually one predominates and this will have an stain. This facilitates the selective staining of different basic tissues and improves the morphological analysis of histo- overall negative or positive charge (being an acid or chemical approaches of the cell components. The VOF-Type III a basic protein). These charges can, however, bal- G.S stain is composed of a mixture of several dyes of varying ance each other out to some degree. Phosphate size and molecular weight (Orange G< acid Fuchsin< Light green<Methyl Blue<Fast Green), which are used simultane- groups of DNA and binding-proteins are important ously, and it enables the individual tissues to be selectively dif- in nuclear staining.The ionisation of basic groups of ferentiated and stained.
    [Show full text]
  • STAINING TECHNIQUES — Staining Is an Auxiliary Technique Used in Microscopy to Enhance Contrast in the Microscopic Image
    STAINING TECHNIQUES Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains or dyes are used in biology and medicine to highlight structures in biological tissues for viewing with microscope. Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. Using different stains, it is possible to stain preferentially certain cell components, such as a nucleus or a cell wall, or the entire cell. Most stains can be used on fixed, or non-living cells, while only some can be used on living cells; some stains can be used on either living or non-living cells. In biochemistry, staining involves adding a class specific (DNA, lipids, proteins or carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescence tagging can serve similar purposes Purposes of Staining The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample. Cells may also be enumerated by staining cells to determine biomass in an environment of interest. Stains may be used to define and examine bulk tissues (e.g. muscle fibers or connective tissues), cell populations (different blood cells) or organelles within individual cells. Biological staining is also used to mark cells in flow cytometry, flag proteins or nucleic acids on gel electrophoresis Staining is not limited to biological materials, it can also be used to study the morphology (form) of other materials e.g.
    [Show full text]
  • 20 to 30 Sec)
    457 Observations on a highly specific method for the histochemical detection of sulphated mucopolysaccharides, and its possible mechanisms By I. D. HEATH (From the Department of Anatomy, University of St. Andrews, Queen's College, Dundee. Present address: General Hospital, Nottingham) With 3 plates (figs, i to 3) Summary Whereas basic dyes in aqueous solutions stain chromatin, all mucins, mast cells, the ground substance of cartilage, and epidermis, it has been shown that a 0-03 % solution of basic dye in 5% aluminium sulphate produces a highly specific staining reaction for sulphated mucopolysaccharides. The best dyes are nuclear fast red (Herzberg) and methylene blue. Acid dyes in solutions of aluminium salts are induced to stain the ground substance of cartilage. These observations have been confirmed in a num- ber of species. Other metallic ions have similar properties and the use of green and purple chromic salts indicate that co-ordination plays a part in the reaction. Methylation, saponification, and sulphation experiments show that the sulphate group is essential. This has been confirmed by using pure chemical substances in gelatin models. Oxidation of keratin with performic acid, which produces sulphonic groups, causes hair (previously negative) to react. From this it is suggested that sul- phonic groups may also react, and that the reactive groups need not be attached to mucopolysaccharides. It is further suggested that the specificity of sulphated muco- polysaccharides is due to the fact that they are the only substances present in the tis- sues with a sufficient concentration of sulphate groups. Experiments with solochrome azurine show that the aluminium is attached to all tissue elements irrespective of their nature.
    [Show full text]
  • Biological Stains & Dyes
    BIOLOGICAL STAINS & DYES Developed for Biology, microbiology & industrial applications ACRIFLAVIN ALCIAN BLUE 8GX ACRIDINE ORANGE ALIZARINE CYANINE GREEN ANILINE BLUE (SPIRIT SOLUBLE) www.lobachemie.com BIOLOGICAL STAINS & DYES Staining is an important technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues. Loba Chemie offers comprehensive range of Stains and dyes, which are frequently used in Microbiology, Hematology, Histology, Cytology, Protein and DNA Staining after Electrophoresis and Fluorescence Microscopy etc. Many of our stains and dyes have specifications complying certified grade of Biological Stain Commission, and suitable for biological research. Stringent testing on all batches is performed to ensure consistency and satisfy necessary specification particularly in challenging work such as histology and molecular biology. Stains and dyes offer by Loba chemie includes Dry – powder form Stains and dyes as well as wet - ready to use solutions. Features: • Ideally suited to molecular biology or microbiology applications • Available in a wide range of innovative chemical packaging options. Range of Biological Stains & Dyes Product Code Product Name C.I. No CAS No 00590 ACRIDINE ORANGE 46005 10127-02-3 00600 ACRIFLAVIN 46000 8063-24-9 00830 ALCIAN BLUE 8GX 74240 33864-99-2 00840 ALIZARINE AR 58000 72-48-0 00852 ALIZARINE CYANINE GREEN 61570 4403-90-1 00980 AMARANTH 16185 915-67-3 01010 AMIDO BLACK 10B 20470
    [Show full text]
  • Mechanistic Insights Into Photodegradation of Organic Dyes Using Heterostructure Photocatalysts
    catalysts Review Mechanistic Insights into Photodegradation of Organic Dyes Using Heterostructure Photocatalysts Yi-Hsuan Chiu 1 , Tso-Fu Mark Chang 2,*, Chun-Yi Chen 2,*, Masato Sone 2 and Yung-Jung Hsu 1,3,* 1 Department of Materials Science and Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan; [email protected] 2 Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503, Japan; [email protected] 3 Center for Emergent Functional Matter Science, National Chiao Tung University, Hsinchu 30010, Taiwan * Correspondence: [email protected] (T.-F.M.C.); [email protected] (C.-Y.C.); [email protected] (Y.-J.H.) Received: 12 April 2019; Accepted: 3 May 2019; Published: 9 May 2019 Abstract: Due to its low cost, environmentally friendly process, and lack of secondary contamination, the photodegradation of dyes is regarded as a promising technology for industrial wastewater treatment. This technology demonstrates the light-enhanced generation of charge carriers and reactive radicals that non-selectively degrade various organic dyes into water, CO2, and other organic compounds via direct photodegradation or a sensitization-mediated degradation process. The overall efficiency of the photocatalysis system is closely dependent upon operational parameters that govern the adsorption and photodegradation of dye molecules, including the initial dye concentration, pH of the solution, temperature of the reaction medium, and light intensity.Additionally, the charge-carrier properties of the photocatalyst strongly affect the generation of reactive species in the heterogeneous photodegradation and thereby dictate the photodegradation efficiency.
    [Show full text]
  • Color Regulatory Affairs Quality Standards for Colors a Safety
    Color Regulatory Affairs Quality Standards for Colors A safety perspective for the brands Sue Ann McAvoy, MS, CFS Sensient Colors LLC September 23, 2015 Why do we use colorants? Effective Color Usage Drives Consumer Trial and Acceptance • …a lasting color impression is made within 90 seconds and accounts for 60 percent of the acceptance or rejection of an object … • Color has a major impact on flavor perception and flavor acceptance. • Color plays a critical role in product identification. Research has indicated that people “see” flavors (Acree 2013, Lewis and others 2008), i.e, they form an opinion about the flavor of a food or beverage, based on its color. What is Permitted Worldwide? Issues Facing the Global Color Industry .Southampton Study .Sustainability Image can be enhanced by renewable natural colors .Ongoing food safety issues .Lack of specific quality and safety specifications in the regulations .On going import detentions involving color Synthetic Raw Materials Under Pressure • Increasing concern and Central Government activity around chemical industry in China and India • Availability of raw materials has become much more volatile • Cost are increasing and needs for environmental controls become more wide spread • Cost could double for synthetic colors over next 2 – 3 years • Natural colors may become an attractive alternatives from a supply security and environmentally responsible standpoint. Trends Towards Color from Naturals Sources in Europe CONFIDENTIAL Global Food Colours Market by Country/Region, 2012 17% 9% 36% Europe
    [Show full text]