Clemson University TigerPrints All Theses Theses 12-2018 System for the Application of Hydrostatic Pressure and Mechanical Strain to Cell Cultures Justin Bacaoat Clemson University,
[email protected] Follow this and additional works at: https://tigerprints.clemson.edu/all_theses Recommended Citation Bacaoat, Justin, "System for the Application of Hydrostatic Pressure and Mechanical Strain to Cell Cultures" (2018). All Theses. 2966. https://tigerprints.clemson.edu/all_theses/2966 This Thesis is brought to you for free and open access by the Theses at TigerPrints. It has been accepted for inclusion in All Theses by an authorized administrator of TigerPrints. For more information, please contact
[email protected]. SYSTEM FOR THE APPLICATION OF HYDROSTATIC PRESSURE AND MECHANICAL STRAIN TO CELL CULTURES A Thesis Presented to the Graduate School of Clemson University In Partial Fulfillment of the Requirements for the Degree Master of Science Bioengineering by Justin Bacaoat December 2018 Accepted by: Jiro Nagatomi, PhD, Committee Chair William Richardson, PhD Ken Webb, PhD ABSTRACT Pressure and stretch are two of the primary forces that result in mechanotransductory events which regulate certain aspects of human health and disease. Laboratory systems such as the commercially available Flexercell® system and a variety of custom-made setups are currently used in research to systematically apply stretch and hydrostatic pressure independently, or in conjunction to cell and tissue cultures. However, these systems do not allow for the decoupling of pressure and stretch under the same culture conditions. The present study aims to design, fabricate, and calibrate a device that can apply pressure and stretch simultaneously, as well as independently to cells in culture.