DOCSLIB.ORG

Desarrollo Y Aplicación De Herramientas Genómicas Para La Mejora De Especies Cucurbitáceas Por Calidad Y Resistencia a Enferm

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Desarrollo Y Aplicación De Herramientas Genómicas Para La Mejora De Especies Cucurbitáceas Por Calidad Y Resistencia a Enferm Desarrollo y aplicación de herramientas genómicas para la mejora de especies cucurbitáceas por calidad y resistencia a enfermedades. TESIS DOCTORAL Presentada por: Cristina Esteras Gómez Dirigida por: Dra. María Belén Picó Sirvent Valencia, mayo 2012 Agradecimientos Quiero expresar mi agradecimiento de forma muy especial a la directora de esta Tesis, la Dra. Belén Picó, por su constante apoyo durante todos estos años, por tantas enseñanzas recibidas y por la total confianza que ha depositado siempre en mí. Así mismo, me gustaría agradecer al Dr. Fernando Nuez, director del COMAV, el haberme dado la posibilidad de realizar los trabajos de la presente Tesis doctoral en el marco de varios proyectos desarrollados en dicho Instituto. Desearía dar las gracias también a todos aquellos que han colaborado de una forma u otra a que saliera adelante este trabajo, en especial a Cristina Roig, que tantas cosas me ha enseñado y con la que tantas horas he pasado en el laboratorio, a José Blanca, Antonio Monforte, Joaquín Cañizares, Pello Ziarsolo y los investigadores de otras instituciones con las que se ha colaborado como el IRTA, IFAPA y la Universidad de Nápoles Federico II. También agradecer todo su apoyo a todos mis compañeros del COMAV, con los que he compartido tantas horas, y en especial a los que “conviven” conmigo en el despacho, a Alicia, Javi y Santi, por sus inestimables consejos siempre que las cosas no salían, su ayuda y sus risas en tantos otros momentos. Y, por supuesto, a todos aquellos que en algún momento u otro han pertenecido al grupo de Cucurbitáceas del COMAV, en especial a Eva y Mercè. Finalmente, mi más profundo agradecimiento a mi familia, sin la cual nada de esto habría sido posible, y en especial a Juanra, por toda su paciencia en los malos momentos. 3 ABSTRACT Melon (Cucumis melo ) and summer squash (Cucurbita pepo ) are economically important cucurbits cultivated worldwide. New, better-adapted, cultivars, resistant to pests and diseases, and varieties displaying better quality traits are required to optimize their production and to meet market demands. Crop breeding must be carried out efficiently and competitively, using the existing genetic knowledge and the molecular tools available for each species. The development of genomic tools to speed up Cucurbits breeding programs is the main objective of this Doctoral Thesis. Molecular markers are essential tools for the construction of genetic maps, for an efficient MAS selection, for QTL (Quantitative Trait Loci ) analysis and mapping and for the development of new breeding populations. Molecular markers are also necessary tools for biodiversity analysis. High-quality markers, such as microsatellites (Simple Sequence Repeats, SSRs) and SNPs (Single Nucleotide Polymorphisms) have been developed and/or validated for both species in this Thesis. The generation of sequence information, required for the development of SSRs and SNPs has dramatically changed in the last 6 years due to the advent of new technological advances. In fact, a fairly complete melon transcriptome has been produced using high-throughput 454 sequencing (NGS, Next Generation Sequencing) and combining the newly produced ESTs with the previously existing Sanger sequences. The melon transcriptome characterization presented is the most complete to date (53.252 unigenes with over 63% of them annotated). The generation of large SSRs and SNPs collections for these species, by mapping the ESTs (Expressed Sequence Tags) against the new version of the transcriptome has been a great achievement starting a new era in melon breeding. The obtained collection of molecular markers (more than 3.000 SSRs and 38.000 SNPs) has allowed the construction of melon maps with increased marker density. 5 Similar resources have been developed for the first time in C. pepo . SSRs identified in the C. pepo transcriptome sequencing project has been applied to localize landraces with favourable commercial traits and whose alleles might be exploited in breeding programs. Likewise, the usefulness of both EST-SSRs and genomic SSRs for depicting genetic relationships has been proved. On the other hand, the employment of the first SNP set identified for Cucurbita through a massive genotyping platform, GoldenGate, has been a success (90%). The genetic map obtained, with more than 300 SNPs, has allowed to map a large set of QTLs related to early flowering, femaleness tendency, shape, size and fruit color, and other traits of interest in breeding. Therefore, these works report the validation and application of previously developed markers and the generation of new genomic tools to speed up the breeding process of these crops. The paper-based format of this Thesis confirms the interest of the obtained results. 6 RESUMEN El melón (Cucumis melo ) y el calabacín ( Cucurbita pepo ) son especies cucurbitáceas de gran importancia económica a nivel nacional y mundial. Para optimizar su producción se requiere de la obtención de nuevas variedades mejor adaptadas a los sistemas de cultivo, más resistentes frente a nuevas enfermedades o plagas y con mejores características organolépticas, que respondan a las cada vez mayores exigencias del mercado. La mejora debe realizarse de una forma eficiente y competitiva, apoyándose en los crecientes conocimientos genéticos en estas dos especies y en los últimos avances biotecnológicos. El desarrollo de herramientas genómicas con el fin de impulsar la mejora de estos cultivos es el principal objetivo de la presente Tesis doctoral. El desarrollo de marcadores moleculares es esencial para la construcción de mapas genéticos, para la realización de una selección más eficiente, para el análisis y cartografía de QTLs (Quantitative Trait Loci ) y para el desarrollo de líneas de premejora, además de ser una herramienta fundamental para el análisis de la biodiversidad. En esta Tesis se han desarrollo y/o validado marcadores de alta calidad, de tipo microsatélite (Simple Sequence Repeats, SSRs) y SNPs (Single Nucleotide Polymorphisms), para estas dos especies. La generación de información de secuencia, necesaria para el desarrollo de este tipo de marcadores, ha cambiado en el transcurso de los trabajos presentados en la Tesis, habiéndose abordado finalmente la secuenciación del transcriptoma de melón mediante técnicas de secuenciación de alto rendimiento (NGS, Next Generation Sequencing). La obtención de grandes colecciones de SSRs y SNPs en ambas especies, resultado del ensamblaje de ESTs (Expressed Sequence Tags) procedentes de secuencias Sanger previamente disponibles y de las nuevas secuencias obtenidas por secuenciación masiva, ha supuesto un gran avance para estas especies no 7 modelo, permitiendo la construcción de mapas más densos en melón y del primer mapa basado en SNPs en calabacín. En melón, la caracterización del transcriptoma presentada es, con 53.252 unigenes, de los cuales el 63% están anotados funcionalmente con términos GO, la más completa que existe. Este transcriptoma ha proporcionado una valiosa información para la anotación del genoma, actualmente en fase de publicación. Además de la identificación de más de 3.000 SSRs y 38.000 SNPs, ha supuesto el desarrollo de herramientas bioinformáticas para la selección de marcadores polimórficos entre diversos morfotipos de interés, lo que abre camino a una nueva era en la mejora de este cultivo. La aplicación de los SSRs identificados gracias a la secuenciación del transcriptoma de C. pepo ha servido para localizar cultivares tradicionales cuya riqueza alélica podría ser explotada con fines comerciales. Asimismo, ha confirmado la eficacia de los EST-SSRs frente a los de origen genómico para estudios de variabilidad. Por otra parte, la utilización de los primeros SNPs identificados para Cucurbita ha supuesto la primera aplicación, con un 90% de éxito, de una plataforma de genotipado masivo, GoldenGate, en este género. El mapa genético basado en más de 300 SNPs ha permitido cartografiar un amplio conjunto de QTLs relacionados con la precocidad de la floración, la tendencia femenina de la planta, la forma, tamaño y coloración del fruto, además de otros caracteres que resultan de gran interés para la mejora. Por tanto, estos trabajos suponen la validación y la aplicación de marcadores previamente desarrollados y la generación de nuevas herramientas genómicas que supondrán la aceleración del proceso de mejora de estos cultivos. El formato de la Tesis basado en artículos ya publicados por el grupo confirma el interés de los resultados obtenidos. 8 RESUM El meló ( Cucumis melo ) i la carabasseta ( Cucurbita pepo ) són espècies cucurbitàcies de gran importància econòmica a nivell nacional i mundial. Per a optimitzar la seua producció es requereix de l´obtenció de noves varietats millor adaptades als sistemes de conreu, més resistents a noves malalties o plagues i amb millors característiques organolèptiques, que responguen a les cada vegada majors exigències del mercat. La millora ha de realitzar-se d´una forma eficient i competitiva, basant-se en els creixents coneiximents genètics en aquestes dues espècies i en els últims avançaments biotecnològics. El desenvolupament de ferramentes genòmiques per a impulsar la millora d´aquests conreus és el principal objectiu de la present Tesi doctoral. El desenvolupament de marcadors moleculars és essencial per a la construcció de mapes genètics, per a la realització d´una selecció més eficient, per a l´anàlisi i cartografia de QTLs (Quantitative Trait Loci ) i per al desenvolupament de línies de premillora, a més de
Load more

Recommended publications
  • Suggested Cultural Practices for Bitter Gourd Narinder P.S
    World Vegetable Center Publication Number: 17-818 International Cooperators’ Guide Suggested Cultural Practices for Bitter Gourd Narinder P.S. Dhillon, Peter Hanson, Wallace Chen, R. Srinivasan, Lawrence Kenyon, Ray-yu Yang, Jen Wen Luoh, Maureen Mecozzi Introduction Taiwan lowlands. Growers may need to modify the practices to suit local soil, weather, pest, and Bitter gourd (Momordica charantia L.) is one disease conditions of the most popular vegetables in Southeast 1 Asia. It is a member of the cucurbit family Climate and soil requirements along with cucumber, squash, watermelon, and Bitter gourd grows well in mean air muskmelon. It provides essential micronutrients temperatures of 24-27 °C and planted in a well such as vitamin A (green variety), vitamin C, drained sandy loam or clay loam soil rich in folate calcium and dietary fiber required for organic matter. Optimum soil pH is 6.0-6.7. It good health. Bitter gourd has been used in is normally grown as an annual crop, but can traditional medicine for managing diabetes and perform as a perennial in areas with mild, frost- other diseases. In the past decade, scientific free winters. The plant thrives in the tropics evidence increasingly has shown that bitter and subtropics from lowland areas to altitudes gourd can contribute to lowering high blood of up to 1,000 m. It is more tolerant to low sugar and high blood pressure, and help in temperatures compared to other gourds, but maintaining a healthy weight. Native to China or cool temperatures will retard growth and frost India, the fast-growing vine is grown throughout will kill the plant.
    [Show full text]
  • Dietary Guidelines for Americans 2005
    Dietary Guidelines for Americans 2005 U.S. Department of Health and Human Services U.S. Department of Agriculture www.healthierus.gov/dietaryguidelines i MESSAGE FROM THE SECRETARIES We are pleased to present the 2005 Dietary Guidelines for Americans. This document is intended to be a primary source of dietary health information for policymakers, nutrition educators, and health providers. Based on the latest scientific evidence, the 2005 Dietary Guidelines provides information and advice for choosing a nutritious diet, maintaining a healthy weight, achieving adequate exercise, and “keeping foods safe” to avoid foodborne illness. This document is based on the recommendations put forward by the Dietary Guidelines Advisory Committee. The Committee was composed of scientific experts who were responsible for reviewing and analyzing the most current dietary and nutritional information and incorporating this into a scientific evidence-based report. We want to thank them and the other public and private professionals who assisted in developing this document for their hard work and dedication. The more we learn about nutrition and exercise, the more we recognize their importance in everyday life. Children need a healthy diet for normal growth and development, and Americans of all ages may reduce their risk of chronic disease by adopting a nutritious diet and engaging in regular physical activity. However, putting this knowledge into practice is difficult. More than 90 million Americans are affected by chronic diseases and conditions that compromise their quality of life and well-being. Overweight and obesity, which are risk factors for diabetes and other chronic diseases, are more common than ever before. To correct this problem, many Americans must make significant changes in their eating habits and lifestyles.
    [Show full text]
  • Refining of the Egusi Locus in Watermelon Using KASP Assays
    Scientia Horticulturae 257 (2019) 108665 Contents lists available at ScienceDirect Scientia Horticulturae journal homepage: www.elsevier.com/locate/scihorti Refining of the egusi locus in watermelon using KASP assays T ⁎ Lucky Paudela, Josh Clevengerb, Cecilia McGregorc, a Institute for Plant Breeding, Genetics & Genomics, University of Georgia, 1111 Plant Sciences Bldg., Athens, GA, 30602, USA b Institute for Plant Breeding, Genetics & Genomics, University of Georgia, 111 Riverbend Rd. CAGT, Athens, GA, 30602, USA c Department of Horticulture and Institute for Plant Breeding, Genetics & Genomics, University of Georgia, 1111 Plant Sciences Bldg., Athens, GA, 30602, USA ARTICLE INFO ABSTRACT Keywords: Egusi watermelon (Citrullus mucosospermus), a close relative of sweet watermelon, is an economically important Citrullus lanatus crop grown in many West African countries for its protein and lipid rich edible seeds. Egusi watermelon seeds Citrullus mucosospermus have a thick, fleshy mucilaginous seed coat layer surrounding the seed coat which is unique to egusi watermelon. Egusi watermelon The egusi seed trait is controlled by a single recessive Mendelian locus, eg, located on chromosome 6 from Seed coat type 6.75 Mb to 11.03 Mb. This region is 4.28 Mb wide and contains 241 candidate genes. The region lacks adequate QTL-seq markers for fine mapping and for marker-assisted selection (MAS) of the egusi trait. In this study, we used QTL- KASP assays seq to validate the position of the eg locus and to identify SNP markers to refine the locus. A genomic region associated with the egusi trait was confirmed on chromosome 6 from 5.25 Mb to 7.85 Mb partially overlapping the previously mapped eg locus.
    [Show full text]
  • Purple Hibiscus
    1 A GLOSSARY OF IGBO WORDS, NAMES AND PHRASES Taken from the text: Purple Hibiscus by Chimamanda Ngozi Adichie Appendix A: Catholic Terms Appendix B: Pidgin English Compiled & Translated for the NW School by: Eze Anamelechi March 2009 A Abuja: Capital of Nigeria—Federal capital territory modeled after Washington, D.C. (p. 132) “Abumonye n'uwa, onyekambu n'uwa”: “Am I who in the world, who am I in this life?”‖ (p. 276) Adamu: Arabic/Islamic name for Adam, and thus very popular among Muslim Hausas of northern Nigeria. (p. 103) Ade Coker: Ade (ah-DEH) Yoruba male name meaning "crown" or "royal one." Lagosians are known to adopt foreign names (i.e. Coker) Agbogho: short for Agboghobia meaning young lady, maiden (p. 64) Agwonatumbe: "The snake that strikes the tortoise" (i.e. despite the shell/shield)—the name of a masquerade at Aro festival (p. 86) Aja: "sand" or the ritual of "appeasing an oracle" (p. 143) Akamu: Pap made from corn; like English custard made from corn starch; a common and standard accompaniment to Nigerian breakfasts (p. 41) Akara: Bean cake/Pea fritters made from fried ground black-eyed pea paste. A staple Nigerian veggie burger (p. 148) Aku na efe: Aku is flying (p. 218) Aku: Aku are winged termites most common during the rainy season when they swarm; also means "wealth." Akwam ozu: Funeral/grief ritual or send-off ceremonies for the dead. (p. 203) Amaka (f): Short form of female name Chiamaka meaning "God is beautiful" (p. 78) Amaka ka?: "Amaka say?" or guess? (p.
    [Show full text]
  • High Tunnel Melon and Watermelon Production
    High Tunnel Melon and Watermelon Production University of Missouri Extension M173 Contents Author Botany 1 Lewis W. Jett, Division of Plant Sciences, University of Missouri-Columbia Cultivar selection 3 Editorial staff Transplant production 4 MU Extension and Agricultural Information Planting in the high tunnel 5 Dale Langford, editor Dennis Murphy, illustrator Row covers 6 On the World Wide Web Soil management and fertilization 6 Find this and other MU Extension publications on the Irrigation 7 Web at http://muextension.missouri.edu Pollination 7 Photographs Pruning 8 Except where noted, photographs are by Lewis W. Jett. Trellising 8 Harvest and yield 9 Marketing 10 Pest management 10 Useful references 14 Melon and watermelon seed sources 15 Sources of high tunnels (hoophouses) 16 For further information, address questions to College of Dr. Lewis W. Jett Agriculture Extension State Vegetable Crops Specialist Food and Natural Division of Plant Sciences Resources University of Missouri Columbia, MO 65211 Copyright 2006 by the University of Missouri Board of Curators E-mail: [email protected] College of Agriculture, Food and Natural Resources High Tunnel Melon and Watermelon Production igh tunnels are low-cost, passive, melo has several botanical subgroups (Table 1). solar greenhouses that use no fossil In the United States, reticulatus and inodorus are Hfuels for heating or venting (Figure commercially grown, while the remaining groups 1). High tunnels can provide many benefits to are grown for niche or local markets. horticulture crop producers: The cantaloupe fruit that most Americans • High tunnels are used to lengthen the are familiar with is not actually a true cantaloupe.
    [Show full text]
  • DRIDIETARY REFERENCE INTAKES Thiamin, Riboflavin, Niacin, Vitamin
    Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline http://www.nap.edu/catalog/6015.html DIETARY REFERENCE INTAKES DRI FOR Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline A Report of the Standing Committee on the Scientific Evaluation of Dietary Reference Intakes and its Panel on Folate, Other B Vitamins, and Choline and Subcommittee on Upper Reference Levels of Nutrients Food and Nutrition Board Institute of Medicine NATIONAL ACADEMY PRESS Washington, D.C. Copyright © National Academy of Sciences. All rights reserved. Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline http://www.nap.edu/catalog/6015.html NATIONAL ACADEMY PRESS • 2101 Constitution Avenue, N.W. • Washington, DC 20418 NOTICE: The project that is the subject of this report was approved by the Governing Board of the National Research Council, whose members are drawn from the councils of the National Academy of Sciences, the National Academy of Engineering, and the Institute of Medicine. The members of the committee responsible for the report were chosen for their special competences and with regard for appropriate balance. This project was funded by the U.S. Department of Health and Human Services Office of Disease Prevention and Health Promotion, Contract No. 282-96-0033, T01; the National Institutes of Health Office of Nutrition Supplements, Contract No. N01-OD-4-2139, T024, the Centers for Disease Control and Prevention, National Center for Chronic Disease Preven- tion and Health Promotion, Division of Nutrition and Physical Activity; Health Canada; the Institute of Medicine; and the Dietary Reference Intakes Corporate Donors’ Fund.
    [Show full text]
  • Reimer Seeds Catalog
    LCTRONICLCTRONIC CATALOGCATALOG Cantaloupes & Melons CA52‐20 ‐ Amarillo Oro Melons CA24‐10 ‐ Ambrosia Melons 100 days. Cucumis melo. Open Pollinated. 86 days. Cucumis melo. (F1) The plant The plant produces good yields of 3 ½ to 5 lb produces high yields of 4 ½ to 5 lb round golden yellow oblong melons and can reach cantaloupes. These eastern type melons 15 lbs. It has a creamy white flesh that is have a terrific extra sweet flavor and peach‐ sweet. A winter‐type melon that is a good colored flesh. It has a nectarous aroma and shipper. An excellent choice for home is very juicy. Melons have small seed gardens and market growers. A pre‐1870 cavities. Ambrosia is recognized as one of heirloom variety from Spain. the best‐tasting melons. An excellent choice for home gardens and market growers. Disease Resistant: DM, PM. CA48‐20 ‐ Amish Melons CA31‐20 ‐ Casaba Golden Beauty Melons 90 days. Cucumis melo. Open Pollinated. 90 days. Cucumis melo. Open Pollinated. The plant produces high yields of 4 to 7 lb Plant produces good yields of 6 to 8 lb cantaloupes. The sweet orange flesh is very golden cantaloupes with dark green juicy and has a muskmelon flavor. It does mottling. The melon has white flesh that is well in most regions of the United States, very sweet. Stores well. Does well in hot dry even in extreme heat. An excellent choice climates. Excellent choice for home gardens for home gardens. An heirloom variety from and market growers. A heirloom variety the Amish community. dating back to the 1920s.
    [Show full text]
  • Citrullus Colocynthis (L.) Schrad
    Emirates Journal of Food and Agriculture. 2017. 29(2): 83-90 doi: 10.9755/ejfa.2016-11-1764 http://www.ejfa.me/ REVIEW ARTICLE Citrullus colocynthis (L.) Schrad. (colocynth): Biotechnological perspectives Jaime A. Teixeira da Silva1*, Abdullah I. Hussain2 1P. O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan, 2Department of Applied Chemistry and Biochemistry, Natural Product and Synthetic Chemistry Laboratory, Government College University, Faisalabad 38000, Pakistan ABSTRACT Citrullus colocynthis (L.) Schrad. is commonly known as colocynth. The fruit pulp of colocynth has medicinal properties while the seeds have nutritive qualities. C. colosynthis is resistant to high temperatures and grows in the desert regions of North Africa, the Middle East and Western Asia. C. colocynthis likely carries genes of interest that could be explored for inducing abiotic stress resistance in transgenic plants. Although the tissue culture and molecular biology of this species have been explored, the latter has been primarily used to resolve taxonomic relationships with other members of the Citrullus genus and curcubits. Genetic mining of the plant is scarce while genetic transformation protocols are also rare. The aim of the present review is to present a brief overview of the biotechnological perspectives of C. colocynthis. Keywords: Abiotic stress-resistance; Colocynth; Cucurbitaceae; Medicinal properties; Plant growth regulators; Tissue culture INTRODUCTION C. lanatus subsp. mucosospermus; Levi and Thomas, 2005), a position supported by morphological and phenetic analyses Citrullus colocynthis (L.) Schrad. (Cucurbitaceae) has medicinal (Achigan-Dako et al., 2015) and genetic studies (Paris, 2016) and ornamental purposes, the former derived primarily (Table 1). Jarret and Newman (2000) also showed that from the fruit pulp (de Smet, 1997).
    [Show full text]
  • Nutritional Composition and Oil Characteristics of Golden Melon (Cucumis Melo ) Seeds
    Food Science and Quality Management www.iiste.org ISSN 2224-6088 (Paper) ISSN 2225-0557 (Online) Vol.27, 2014 Nutritional Composition and Oil Characteristics of Golden Melon (Cucumis melo ) Seeds Oluwatoyin H. Raji * Oluwaseun T. Orelaja Department of Food Technology, Moshood Abiola Polytechnic Abeokuta, P.O.box 2210 Abeokuta, Ogun state * E-mail of the corresponding author: [email protected] Abstract This study investigated the mineral and proximate composition of Golden/canary melon ( Cucumis melo ) seeds and the physiochemical properties of the seed oil. Proximate composition and physicochemical properties of oil were performed according to AOAC procedures. Minerals were determined using the method of Novozamsky et al. (1983). Results show that the seeds contained high percentage of crude fibre (33.94%) and low percentage of carbohydrate (3.14%). The seeds also contain high value of iron (136.5ppm), zinc (48.35ppm), manganese (25.70ppm), copper (15.40ppm) and low value of calcium (0.023±0.001%). Hexane extracted oil had acid value (2.68mgKOH/g) peroxide value (7.42mgKOH/g), iodine value of (117.43mgKOH/g), saponification value (191.42), free fatty acid (2.34) moisture content (5.68%), and refractive index (1.62) respectively. The seeds serve as good sources of crude fiber, fat and protein. Results also showed that the golden/canary melon oil is non rancid. Keywords: Physicochemical, Golden melon, Hexane extracted oil 1. Introduction Cucurbitaceae (Cucurbit) is an important family comprising one of the most genetically diverse groups of food plants. Most of the plants belonging to this family are frost sensitive and drought-tolerant (Whitaker and Bohn, 1950).
    [Show full text]
  • Generate Evaluation Form
    Great Lakes Fruit, Vegetable & Farm Market EXPO Michigan Greenhouse Growers EXPO December 5-7, 2017 DeVos Place Convention Center, Grand Rapids, MI Vine Crops Where: Grand Gallery (main level) Room E & F MI Recertification credits: 2 (1B, COMM CORE, PRIV CORE) OH Recertification credits: 1 (presentations as marked) CCA Credits: PM(1.0) CM(1.0) Moderator: Ben Phillips, Vegetable Extension Educator, MSU Extension, Saginaw, MI 9:00 am Integrated Management of Powdery Mildew and Gummy Stem Blight on Cucurbits: A Florida Perspective (OH: 2B, 0.5 hr) Gary Vallad, Plant Pathology Dept., Univ. of Florida 9:30 am Perspectives and Opportunities for Growing Orange Flesh and Specialty Melons Jonathan Schultheis, Dept. Horticultural Science, North Carolina State University 10:00 am Best Management Practices: Controlling Insects While Protecting Pollinators (OH: CORE, 0.5 hr) Rick Foster, Entomology Dept., Purdue Univ. 10:30 am Cover Cropping in Melons for Reduced Washing After Harvest Chris Gunter, Horticultural Science Dept., North Carolina State Univ. 11:00 am Session Ends Perspectives and Opportunities for Growing Orange Flesh and Specialty Melons Jonathan R. Schultheis North Carolina State University Department of Horticultural Science 2721 Founders Drive, 264 Kilgore Raleigh, NC 27695-7609 [email protected] Orange Flesh Melon Types: The landscape of orange flesh muskmelons (cantaloupes) has changed dramatically over the past five to ten years. Traditionally, there were two primary orange flesh melon types grown and sold; western types, which are fruits that range in size from about 3 to 5 pounds which are heavily netted and not sutured, and eastern types which have some netting, have a shorter shelf life but have a larger fruit size than a western melon, and tend to have a softer flesh with apparent more flavor than a western melon.
    [Show full text]
  • Pyrolysis Kinetics of Melon (Citrullus Colocynthis L.) Seed Husk
    Pyrolysis kinetics of Melon (Citrullus colocynthis L.) seed husk Bemgba Bevan Nyakuma Centre for Hydrogen Energy, Institute of Future Energy, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia. *Corresponding author Email: [email protected], [email protected] Abstract This study is aimed at investigating the thermochemical fuel characteristics and kinetic decomposition of melon seed husks (MSH) under inert (pyrolysis) conditions. The calorific value, elemental composition, proximate analyses and thermal kinetics of MSH was examined. The kinetic parameters; activation energy E and frequency factor A for MSH decomposition under pyrolysis conditions were determined using the Kissinger and isoconversional Flynn-Wall-Ozawa (FWO) methods. The values of E for MSH ranged from 146.81 to 296 kJ/mol at degrees of conversion α = 0.15 to 0.60 for FWO. The decomposition of MSH process was fastest at α = 0.15 and slowest at α = 0.60 with average E and A values of 192.96 kJ/mol and 2.86 x 1026 min-1, respectively at correlation values of 0.9847. The kinetic values of MSH using the Kissinger method are E = 161.26 kJ/mol and frequency factor, A = 2.08 x 1010 min-1 with the correlation value, R2 = 0.9958. The results indicate that MSH possesses important characteristics of a potential solid biofuel (SBF) for future thermochemical applications in clean energy and power generation. Keywords: Biofuel, Pyrolysis, Kinetics, Melon, Seed, Husks Introduction Melon (Citrullus colocynthis L.) is an important oil seed and perennial cash crop widely cultivated in many parts of Africa. The vegetable oil extracted from melon seed is primarily used for domestic consumption although medicinal uses [1, 2] and industrial applications for biodiesel [3, 4], soap, detergents and margarine production, have been reported.
    [Show full text]
  • Ck01e Index.Qxp 4/24/2006 2:09 PM Page 258 Ck01e Index.Qxp 4/24/2006 2:09 PM Page 259
    ck01e index.qxp 4/24/2006 2:09 PM Page 258 ck01e index.qxp 4/24/2006 2:09 PM Page 259 Index BOTANICAL LATIN NAMES Citrofortunella mitis J. Ingram and H. E. Feijoa sellowiana, 109 Moore, 121 Ficus carica, 66 Abelmoschus manihot, 239 Citrus aurantium Linn, 123 Acer saccharum, 206 Citrus aurantium var. grandis, 132 Ganoderma lucidum, 56 Achras zapota, L., 204 Citrus bigaradia Risso, 123 Garcinia mangostana Linn., 206 Actinidia arguta, 190 Citrus decumana, 132 Gaylussacia, 63 Actinidia chinensis, 190 Citrus grandis, 132 Gelidium cartilagineum Gaill., 202 Actinidia deliciosa, 190 Citrus maxima (Burm.) Merr., 132 Gelidium corneum Lam., 202 Actinidia species, 190 Citrus mitis Blanco, 121 Glycyrrhiza glabra, 56 Aloysia triphylla, 161-62 Citrus reticulata, 120, 123 Amelanchier bartramiana, 208 Citrus sinensis (L.) Osbeck, 123, 129, 181 Hibiscus sabdariffa Linn., 207, 238 Amelanchier sanguinea (Purch.) D C, Citrus sinensis ‘Maltese’, 181 Hylocereus undatus, 199 207 Coccoloba uvifera, 208 Amelanchier sanguinea var. alnifolia, 208 Cucumis metuliferus, 205 Ipomoea batatas, 234 Angelica archangelica, 134, 176 Cucurbitaceae, 169 Annona cherimola Mill., 208 Cucurbita pepo Linn., 169 Lansium domesticum, 205 Annona muricata, 208, 239 Cucurbita pepo melopepo, 169 Lippia citriodora, 161-62 Annona reticulate, 239 Cucurbita pepo pepo, 169 Litchi chinensis Sonn., 205 Annona squamosa, 208, 239 Cucurbita pepos, 171 Anthemis nobilis, 128-29 Cucurbita species, 173 Malpighia punicifolia Linn., 238 Anthriscus cerefolium (L.) Hoffm., 200 Cucurbita texana, 169 Mammea
    [Show full text]