RPS27A Antibody Cat. No.: 18-452

RPS27A Antibody

Immunofluorescence analysis of U2OS cells using RPS27A antibody (18-452) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of IMMUNOGEN: human RPS27A (NP_001170884.1).

TESTED APPLICATIONS: IF, WB

WB: ,1:500 - 1:2000 APPLICATIONS: IF: ,1:50 - 1:200

POSITIVE CONTROL: 1) Jurkat

2) NIH/3T3

September 28, 2021 1 https://www.prosci-inc.com/rps27a-antibody-18-452.html 3) Mouse kidney

4) Mouse liver

5) Rat liver

PREDICTED MOLECULAR Observed: 14kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: RPS27A

UBA80, UBCEP1, Ubiquitin-40S ribosomal protein S27a, Ubiquitin carboxyl extension ALTERNATE NAMES: protein 80UBC, S27A, CEP80, HEL112, UBCEP80

GENE ID: 6233

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post- translationally processed, generating free ubiquitin monomer and ribosomal protein BACKGROUND: S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

September 28, 2021 2 https://www.prosci-inc.com/rps27a-antibody-18-452.html ANTIBODIES FOR RESEARCH USE ONLY.

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September 28, 2021 3 https://www.prosci-inc.com/rps27a-antibody-18-452.html