RPS27A Antibody Cat. No.: 18-452 RPS27A Antibody Immunofluorescence analysis of U2OS cells using RPS27A antibody (18-452) at dilution of 1:100. Blue: DAPI for nuclear staining. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of IMMUNOGEN: human RPS27A (NP_001170884.1). TESTED APPLICATIONS: IF, WB WB: ,1:500 - 1:2000 APPLICATIONS: IF: ,1:50 - 1:200 POSITIVE CONTROL: 1) Jurkat 2) NIH/3T3 September 28, 2021 1 https://www.prosci-inc.com/rps27a-antibody-18-452.html 3) Mouse kidney 4) Mouse liver 5) Rat liver PREDICTED MOLECULAR Observed: 14kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: RPS27A UBA80, UBCEP1, Ubiquitin-40S ribosomal protein S27a, Ubiquitin carboxyl extension ALTERNATE NAMES: protein 80UBC, S27A, CEP80, HEL112, UBCEP80 GENE ID: 6233 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post- translationally processed, generating free ubiquitin monomer and ribosomal protein BACKGROUND: S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified. September 28, 2021 2 https://www.prosci-inc.com/rps27a-antibody-18-452.html ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 28, 2021 3 https://www.prosci-inc.com/rps27a-antibody-18-452.html.