Envis Bulletin Himalayan Ecology
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Phylogenetic Relationship and Evolution Analysis of the Peony Paeonia Species Using Multi-Locus Deoxyribonucleic Acid (DNA) Barcodes
Journal of Medicinal Plants Research Vol. 6(37), pp. 5048-5058, 26 September, 2012 Available online at http://www.academicjournals.org/JMPR DOI: 10.5897/JMPR11.1733 ISSN 1996-0875 ©2012 Academic Journals Full Length Research Paper Phylogenetic relationship and evolution analysis of the peony Paeonia species using multi-locus deoxyribonucleic acid (DNA) barcodes Yan-Lin Sun1,2 and Soon-Kwan Hong2,3* 1School of Life Sciences, Ludong University, Yantai, Shandong, 264-025, China. 2Department of Bio-Health Technology, Kangwon National University, Chuncheon, Kangwon, 200-701, Korea. 3Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Kangwon, 200-701, Korea. Accepted 6 July, 2012 Paeonia is a phylogenetically and taxonomically complex group. In this study, two chloroplast coding region of the matK and rbcL genes, two intergenic spacers, 5’ trnK-matK and matK-3’ trnK, the ribosomal RNA 18S and 5S genes, and the nuclear ribosomal DNA ITS gene were sequenced to study phylogenetic relationships of four Paeonia species. In the matK coding region, most nucleotide variations pointed at the divergence between Paeonia suffruticosa (MO) and other three species, strongly supporting the phylogenetic evolution of section Paeonia and section Moutan in the Paeonia genus; the variable sites of Paeonia lactiflora CHA1 accession compared to CHA accession were all identical to Paeonia obovata SHAN accession, indicating that CHA1 might be a hybrid result of CHA and SHAN. Another chloroplast coding region, rbcL showed lower variation rate and less phylogenetic information than the matK region in all four Paeonia species investigated in this study, while the 5S rRNA gene and nrDNA ITS region showed higher variation rate. -
Preliminary Investigation of Different Extracts of Curcuma Caesia for Its
Journal of Pharmacognosy and Phytochemistry 2015; 4(2): 116-120 E-ISSN: 2278-4136 P-ISSN: 2349-8234 Preliminary investigation of different extracts of JPP 2015; 4(2): 116-120 Received: 21-05-2015 Curcuma caesia for its antibacterial effects and Accepted: 23-06-2015 cytotoxic activity Suraj Shakya Nobel College, Faculty of Health Science, Suraj Shakya, Anil Giri, Pratima Chhetri, Sujana Shakya Department of Pharmacy, Kathmandu-Nepal. Abstract The present study is carried out with the different (Petroleum ether, Diethyl ether, Chloroform and Anil Giri Methanol) extracts of dried rootstocks and rhizome of Curcuma caesia. Traditional use of it as liver Nobel College, tonic, antifungal, pain reliever as well as anthelmintic inspired us to investigate the antimicrobial and Faculty of Health Science, Department of Pharmacy, cytotoxic activity of this plant. Preliminary phytochemical screening revealed the presence of alkaloids, Kathmandu-Nepal. terpenoids, flavonoids, deoxysugars as well as C-glycosides. The present study revealed that the tannin was absent in all extract of C. caesia, which may be a possible reason for not showing any antibacterial Pratima Chhetri activity. Also the brine shrimp bioassay revealed that petroleum ether extract, diethyl ether extract, Nobel College, chloroform extract and methanol extract showed LC50 value of 18.923 mcg/ml, 1.086 mcg/ml, 45.289 Faculty of Health Science, mcg/ml and 100 mcg/ml respectively and since the LC50 values less than 1000 mcg/ml indicate the Department of Pharmacy, cytotoxic property of extracts, all the extracts of the plant were biologically active. Kathmandu-Nepal. Keywords: Curcuma caesia, phytochemical screening, Antibacterial activity, cytotoxic activity. -
Climate-Glacier Relationship in the Monsoon-Arid Transition Zone: a Case Study in Himachal Pradesh, India
Climate-Glacier relationship in the monsoon-arid transition zone : A Case study in Himachal Pradesh, India Farooq Azam Mohd To cite this version: Farooq Azam Mohd. Climate-Glacier relationship in the monsoon-arid transition zone : A Case study in Himachal Pradesh, India. Earth Sciences. Université de Grenoble, 2014. English. NNT : 2014GRENU032. tel-01230980 HAL Id: tel-01230980 https://tel.archives-ouvertes.fr/tel-01230980 Submitted on 19 Nov 2015 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THESIS Submitted to obtain the degree of DOCTOR OF THE UNIVERSITY OF GRENOBLE Speciality : Earth Sciences, Universe and Environment Arrêté ministériel : 1 November 2011 Presented by Mohd Farooq AZAM Thesis directed by Patrick Wagnon and co-directed by Christian Vincent & Ramanathan Alagappan Prepared in the Laboratoire de Glaciologie et de Géophysique de l’Environnement/Laboratoire d'Etudes des Transferts en Hydrologie et Environnement, UJF/CNRS in Doctoral school Earth Sciences, Universe and Environment Climate-Glacier relationship in the monsoon-arid transition zone: A Case study in Himachal Pradesh, India. Defended publicly : 17 December 2014, Before the jury : Mr. Gerhard KRINNER Research Director, CNRS, LGGE (France), President Mr. Martin HOELZLE Professor, University of Fribourg (Switzerland), Reporter Mr. -
Peziza and Pezizaceae Inferred from Multiple Nuclear Genes: RPB2, -Tubulin, and LSU Rdna
Molecular Phylogenetics and Evolution 36 (2005) 1–23 www.elsevier.com/locate/ympev Evolutionary relationships of the cup-fungus genus Peziza and Pezizaceae inferred from multiple nuclear genes: RPB2, -tubulin, and LSU rDNA Karen Hansen ¤, Katherine F. LoBuglio, Donald H. PWster Harvard University Herbaria, Cambridge, MA 02138, USA Received 5 May 2004; revised 17 December 2004 Available online 22 April 2005 Abstract To provide a robust phylogeny of Pezizaceae, partial sequences from two nuclear protein-coding genes, RPB2 (encoding the sec- ond largest subunit of RNA polymerase II) and -tubulin, were obtained from 69 and 72 specimens, respectively, to analyze with nuclear ribosomal large subunit RNA gene sequences (LSU). The three-gene data set includes 32 species of Peziza, and 27 species from nine additional epigeous and six hypogeous (truZe) pezizaceous genera. Analyses of the combined LSU, RPB2, and -tubulin data set using parsimony, maximum likelihood, and Bayesian approaches identify 14 Wne-scale lineages within Pezizaceae. Species of Peziza occur in eight of the lineages, spread among other genera of the family, conWrming the non-monophyly of the genus. Although parsimony analyses of the three-gene data set produced a nearly completely resolved strict consensus tree, with increased conWdence, relationships between the lineages are still resolved with mostly weak bootstrap support. Bayesian analyses of the three- gene data, however, show support for several more inclusive clades, mostly congruent with Bayesian analyses of RPB2. No strongly supported incongruence was found among phylogenies derived from the separate LSU, RPB2, and -tubulin data sets. The RPB2 region appeared to be the most informative single gene region based on resolution and clade support, and accounts for the greatest number of potentially parsimony informative characters within the combined data set, followed by the LSU and the -tubulin region. -
Table of Contents
Table of Contents Acknowledgements xi Foreword xii I. EXECUTIVE SUMMARY XIV II. INTRODUCTION 20 A. The Context of the SoE Process 20 B. Objectives of an SoE 21 C. The SoE for Uttaranchal 22 D. Developing the framework for the SoE reporting 22 Identification of priorities 24 Data collection Process 24 Organization of themes 25 III. FROM ENVIRONMENTAL ASSESSMENT TO SUSTAINABLE DEVELOPMENT 34 A. Introduction 34 B. Driving forces and pressures 35 Liberalization 35 The 1962 War with China 39 Political and administrative convenience 40 C. Millennium Eco System Assessment 42 D. Overall Status 44 E. State 44 F. Environments of Concern 45 Land and the People 45 Forests and biodiversity 45 Agriculture 46 Water 46 Energy 46 Urbanization 46 Disasters 47 Industry 47 Transport 47 Tourism 47 G. Significant Environmental Issues 47 Nature Determined Environmental Fragility 48 Inappropriate Development Regimes 49 Lack of Mainstream Concern as Perceived by Communities 49 Uttaranchal SoE November 2004 Responses: Which Way Ahead? 50 H. State Environment Policy 51 Institutional arrangements 51 Issues in present arrangements 53 Clean Production & development 54 Decentralization 63 IV. LAND AND PEOPLE 65 A. Introduction 65 B. Geological Setting and Physiography 65 C. Drainage 69 D. Land Resources 72 E. Soils 73 F. Demographical details 74 Decadal Population growth 75 Sex Ratio 75 Population Density 76 Literacy 77 Remoteness and Isolation 77 G. Rural & Urban Population 77 H. Caste Stratification of Garhwalis and Kumaonis 78 Tribal communities 79 I. Localities in Uttaranchal 79 J. Livelihoods 82 K. Women of Uttaranchal 84 Increased workload on women – Case Study from Pindar Valley 84 L. -
A Four-Locus Phylogeny of Rib-Stiped Cupulate Species Of
A peer-reviewed open-access journal MycoKeys 60: 45–67 (2019) A four-locus phylogeny of of Helvella 45 doi: 10.3897/mycokeys.60.38186 RESEARCH ARTICLE MycoKeys http://mycokeys.pensoft.net Launched to accelerate biodiversity research A four-locus phylogeny of rib-stiped cupulate species of Helvella (Helvellaceae, Pezizales) with discovery of three new species Xin-Cun Wang1, Tie-Zhi Liu2, Shuang-Lin Chen3, Yi Li4, Wen-Ying Zhuang1 1 State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China 2 College of Life Sciences, Chifeng University, Chifeng, Inner Mongolia 024000, China 3 College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, China 4 College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China Corresponding author: Wen-Ying Zhuang ([email protected]) Academic editor: T. Lumbsch | Received 11 July 2019 | Accepted 18 September 2019 | Published 31 October 2019 Citation: Wang X-C, Liu T-Z, Chen S-L, Li Y, Zhuang W-Y (2019) A four-locus phylogeny of rib-stiped cupulate species of Helvella (Helvellaceae, Pezizales) with discovery of three new species. MycoKeys 60: 45–67. https://doi. org/10.3897/mycokeys.60.38186 Abstract Helvella species are ascomycetous macrofungi with saddle-shaped or cupulate apothecia. They are distri- buted worldwide and play an important ecological role as ectomycorrhizal symbionts. A recent multi-locus phylogenetic study of the genus suggested that the cupulate group of Helvella was in need of comprehen- sive revision. In this study, all the specimens of cupulate Helvella sensu lato with ribbed stipes deposited in HMAS were examined morphologically and molecularly. -
Gori River Basin Substate BSAP
A BIODIVERSITY LOG AND STRATEGY INPUT DOCUMENT FOR THE GORI RIVER BASIN WESTERN HIMALAYA ECOREGION DISTRICT PITHORAGARH, UTTARANCHAL A SUB-STATE PROCESS UNDER THE NATIONAL BIODIVERSITY STRATEGY AND ACTION PLAN INDIA BY FOUNDATION FOR ECOLOGICAL SECURITY MUNSIARI, DISTRICT PITHORAGARH, UTTARANCHAL 2003 SUBMITTED TO THE MINISTRY OF ENVIRONMENT AND FORESTS GOVERNMENT OF INDIA NEW DELHI CONTENTS FOREWORD ............................................................................................................ 4 The authoring institution. ........................................................................................................... 4 The scope. .................................................................................................................................. 5 A DESCRIPTION OF THE AREA ............................................................................... 9 The landscape............................................................................................................................. 9 The People ............................................................................................................................... 10 THE BIODIVERSITY OF THE GORI RIVER BASIN. ................................................ 15 A brief description of the biodiversity values. ......................................................................... 15 Habitat and community representation in flora. .......................................................................... 15 Species richness and life-form -
Three Challenges to Contemporaneous Taxonomy from a Licheno-Mycological Perspective
Megataxa 001 (1): 078–103 ISSN 2703-3082 (print edition) https://www.mapress.com/j/mt/ MEGATAXA Copyright © 2020 Magnolia Press Review ISSN 2703-3090 (online edition) https://doi.org/10.11646/megataxa.1.1.16 Three challenges to contemporaneous taxonomy from a licheno-mycological perspective ROBERT LÜCKING Botanischer Garten und Botanisches Museum, Freie Universität Berlin, Königin-Luise-Straße 6–8, 14195 Berlin, Germany �[email protected]; https://orcid.org/0000-0002-3431-4636 Abstract Nagoya Protocol, and does not need additional “policing”. Indeed, the Nagoya Protocol puts the heaviest burden on This paper discusses three issues that challenge contempora- taxonomy and researchers cataloguing biodiversity, whereas neous taxonomy, with examples from the fields of mycology for the intended target group, namely those seeking revenue and lichenology, formulated as three questions: (1) What is gain from nature, the protocol may not actually work effec- the importance of taxonomy in contemporaneous and future tively. The notion of currently freely accessible digital se- science and society? (2) An increasing methodological gap in quence information (DSI) to become subject to the protocol, alpha taxonomy: challenge or opportunity? (3) The Nagoya even after previous publication, is misguided and conflicts Protocol: improvement or impediment to the science of tax- with the guidelines for ethical scientific conduct. Through onomy? The importance of taxonomy in society is illustrated its implementation of the Nagoya Protocol, Colombia has using the example of popular field guides and digital me- set a welcome precedence how to exempt taxonomic and dia, a billion-dollar business, arguing that the desire to name systematic research from “access to genetic resources”, and species is an intrinsic feature of the cognitive component of hopefully other biodiversity-rich countries will follow this nature connectedness of humans. -
An Evolving Phylogenetically Based Taxonomy of Lichens and Allied Fungi
Opuscula Philolichenum, 11: 4-10. 2012. *pdf available online 3January2012 via (http://sweetgum.nybg.org/philolichenum/) An evolving phylogenetically based taxonomy of lichens and allied fungi 1 BRENDAN P. HODKINSON ABSTRACT. – A taxonomic scheme for lichens and allied fungi that synthesizes scientific knowledge from a variety of sources is presented. The system put forth here is intended both (1) to provide a skeletal outline of the lichens and allied fungi that can be used as a provisional filing and databasing scheme by lichen herbarium/data managers and (2) to announce the online presence of an official taxonomy that will define the scope of the newly formed International Committee for the Nomenclature of Lichens and Allied Fungi (ICNLAF). The online version of the taxonomy presented here will continue to evolve along with our understanding of the organisms. Additionally, the subfamily Fissurinoideae Rivas Plata, Lücking and Lumbsch is elevated to the rank of family as Fissurinaceae. KEYWORDS. – higher-level taxonomy, lichen-forming fungi, lichenized fungi, phylogeny INTRODUCTION Traditionally, lichen herbaria have been arranged alphabetically, a scheme that stands in stark contrast to the phylogenetic scheme used by nearly all vascular plant herbaria. The justification typically given for this practice is that lichen taxonomy is too unstable to establish a reasonable system of classification. However, recent leaps forward in our understanding of the higher-level classification of fungi, driven primarily by the NSF-funded Assembling the Fungal Tree of Life (AFToL) project (Lutzoni et al. 2004), have caused the taxonomy of lichen-forming and allied fungi to increase significantly in stability. This is especially true within the class Lecanoromycetes, the main group of lichen-forming fungi (Miadlikowska et al. -
Lichen Functional Trait Variation Along an East-West Climatic Gradient in Oregon and Among Habitats in Katmai National Park, Alaska
AN ABSTRACT OF THE THESIS OF Kaleigh Spickerman for the degree of Master of Science in Botany and Plant Pathology presented on June 11, 2015 Title: Lichen Functional Trait Variation Along an East-West Climatic Gradient in Oregon and Among Habitats in Katmai National Park, Alaska Abstract approved: ______________________________________________________ Bruce McCune Functional traits of vascular plants have been an important component of ecological studies for a number of years; however, in more recent times vascular plant ecologists have begun to formalize a set of key traits and universal system of trait measurement. Many recent studies hypothesize global generality of trait patterns, which would allow for comparison among ecosystems and biomes and provide a foundation for general rules and theories, the so-called “Holy Grail” of ecology. However, the majority of these studies focus on functional trait patterns of vascular plants, with a minority examining the patterns of cryptograms such as lichens. Lichens are an important component of many ecosystems due to their contributions to biodiversity and their key ecosystem services, such as contributions to mineral and hydrological cycles and ecosystem food webs. Lichens are also of special interest because of their reliance on atmospheric deposition for nutrients and water, which makes them particularly sensitive to air pollution. Therefore, they are often used as bioindicators of air pollution, climate change, and general ecosystem health. This thesis examines the functional trait patterns of lichens in two contrasting regions with fundamentally different kinds of data. To better understand the patterns of lichen functional traits, we examined reproductive, morphological, and chemical trait variation along precipitation and temperature gradients in Oregon. -
Conservation of Curcuma Caesia by in Vitro Techniques Wahengbam R
Helix Vol.3-4: 708-713 (2015) Conservation of Curcuma caesia by in vitro techniques Wahengbam R. C. Singh1, Heigrujam B. Singh1, Shamurailatpam Surodhani Devi1, Wahengbam N. Singh2, Nongmaithem M. Singh3, Yendrembam P. Devi3 1DBT-Biotech Hub, Department of Biotechnology, S. Kula Women’s College, Nambol,2 DBT- Biotech Hub, K. P. College, Hiyangthang, Manipur,3DBT-Biotech Hub, Pettigrew College, Ukhrul, Manipur Received: 5th April 2015, Accepted: 12th April 2015, Published: 1st July 2015 Abstract Black turmeric or black zedoary (Curcuma This study was conducted to develop an efficient caesia) is a perennial herb with bluish-black protocol for mass propagation of Curcuma caesia rhizome, native to North-East and Central India. L. Explants from underground rhizomes were The rhizome of black turmeric has a high economic cultured on Murashige and Skoog (MS) medium importance owing to its putative medicinal and it is a slow propagating species. Multiplication properties. In west Bengal, the rhizome of the plant and callus induction starting from the rhizome buds is used in Kali Puja, and hence the plant is called and shoot tips of Curcuma caesia in MS medium Kali haldi. By etymology, Kali is the feminine supplemented with NAA; 0.9 mg/l with kinetin; 0.9 form of Kala, which means black color and hence mg/l or NAA; 1.2 mg/l with 6-Benzylaminopurine the plant is termed as black turmeric in English. (BAP; 1.9 mg/l) was the optimum for rapid clonal This species has been regarded as endangered by propagation of Curcuma caesia. Callus induction the central forest department of India due to was conducted with 2,4-dichlorophenoxy-acetic biopiracy. -
A Synopsis of the Saddle Fungi (Helvella: Ascomycota) in Europe – Species Delimitation, Taxonomy and Typification
Persoonia 39, 2017: 201–253 ISSN (Online) 1878-9080 www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE https://doi.org/10.3767/persoonia.2017.39.09 A synopsis of the saddle fungi (Helvella: Ascomycota) in Europe – species delimitation, taxonomy and typification I. Skrede1,*, T. Carlsen1, T. Schumacher1 Key words Abstract Helvella is a widespread, speciose genus of large apothecial ascomycetes (Pezizomycete: Pezizales) that are found in terrestrial biomes of the Northern and Southern Hemispheres. This study represents a beginning on molecular phylogeny assessing species limits and applying correct names for Helvella species based on type material and specimens in the Pezizales university herbaria (fungaria) of Copenhagen (C), Harvard (FH) and Oslo (O). We use morphology and phylogenetic systematics evidence from four loci – heat shock protein 90 (hsp), translation elongation factor alpha (tef), RNA polymerase II (rpb2) and the nuclear large subunit ribosomal DNA (LSU) – to assess species boundaries in an expanded sample of Helvella specimens from Europe. We combine the morphological and phylogenetic information from 55 Helvella species from Europe with a small sample of Helvella species from other regions of the world. Little intraspecific variation was detected within the species using these molecular markers; hsp and rpb2 markers provided useful barcodes for species delimitation in this genus, while LSU provided more variable resolution among the pertinent species. We discuss typification issues and identify molecular characteristics for 55 European Helvella species, designate neo- and epitypes for 30 species, and describe seven Helvella species new to science, i.e., H. alpicola, H. alpina, H. carnosa, H. danica, H. nannfeldtii, H. pubescens and H.