Orchid Research Newsletter 73 (PDF)
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CRIOPRESERVAÇÃO DE ORQUÍDEAS BRASILEIRAS DO GÊNERO Cattleya
CRIOPRESERVAÇÃO DE ORQUÍDEAS BRASILEIRAS DO GÊNERO Cattleya RENATO GOBBI VETTORAZZI UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO - UENF CAMPOS DOS GOYTACAZES - RJ FEVEREIRO - 2020 CRIOPRESERVAÇÃO DE ORQUÍDEAS BRASILEIRAS DO GÊNERO Cattleya RENATO GOBBI VETTORAZZI “Tese apresentada ao Centro de Ciências e Tecnologias Agropecuárias da Universidade Estadual do Norte Fluminense Darcy Ribeiro, como parte das exigências para obtenção do título de Doutor em Genética e Melhoramento de Plantas” Orientadora: Prof.ª Virginia Silva Carvalho CAMPOS DOS GOYTACAZES - RJ FEVEREIRO – 2020 FICHA CATALOGRÁFICA UENF - Bibliotecas Elaborada com os dados fornecidos pelo autor. V592 Vettorazzi, Renato Gobbi. Criopreservação de orquídeas brasileiras do gênero Cattleya / Renato Gobbi Vettorazzi. - Campos dos Goytacazes, RJ, 2020. 118 f. : il. Bibliografia: 80 - 93. Tese (Doutorado em Genética e Melhoramento de Plantas) - Universidade Estadual do Norte Fluminense Darcy Ribeiro, Centro de Ciências e Tecnologias Agropecuárias, 2020. Orientadora: Virginia Silva Carvalho. 1. Orchidaceae. 2. Banco Criogênico. 3. Vitrificação. 4. Manutenção da Diversidade. 5. Conservação de Germoplasma. I. Universidade Estadual do Norte Fluminense Darcy Ribeiro. II. Título. CDD - 631.5233 AGRADECIMENTOS A Deus, pela proteção, graças e bênçãos concedidas; À minha esposa Milena, por não medir esforços em ajudar, pelo companheirismo, carinho, paciência, compreensão e, sobretudo, pelo amor sempre presente; À minha família, pelas orações e por me fazer uma pessoa digna. Em especial, -
Checklist of the Orchids of the Crimea (Orchidaceae)
J. Eur. Orch. 46 (2): 407 - 436. 2014. Alexander V. Fateryga and Karel C.A.J. Kreutz Checklist of the orchids of the Crimea (Orchidaceae) Keywords Orchidaceae, checklist of species, new nomenclature combinations, hybrids, flora of the Crimea. Summary Fateryga, A.V. & C.A.J. Kreutz (2014): Checklist of the orchids of the Crimea (Orchidaceae).- J. Eur. Orch. 46 (2): 407-436. A new nomenclature checklist of the Crimean orchids with 49 taxa and 16 hybrids is proposed. Six new taxa are added and ten taxa are excluded from the latest checklist of the Crimean vascular flora published by YENA (2012). In addition, five nomenclature changes are proposed: Epipactis persica (Soó) Nannf. subsp. taurica (Fateryga & Kreutz) Fateryga & Kreutz comb. et stat. nov., Orchis mascula (L.) L. var. wanjkovii (E. Wulff) Fateryga & Kreutz stat. nov., Anacamptis ×simorrensis (E.G. Camus) H. Kretzschmar, Eccarius & H. Dietr. nothosubsp. ticinensis (Gsell) Fateryga & Kreutz stat. nov., ×Dactylocamptis uechtritziana (Hausskn.) B. Bock ex M. Peregrym & Kuzemko nothosubsp. magyarii (Soó) Fateryga & Kreutz comb. et stat. nov., and Orchis ×beyrichii Kern. nothosubsp. mackaensis (Kreutz) Fateryga & Kreutz comb. et stat. nov. Moreover, a new variety, Limodorum abortivum (L.) Sw. var. viridis Fateryga & Kreutz var. nov. is described. Zusammenfassung Fateryga, A.V. & C.A.J. Kreutz (2014): Eine Übersicht der Orchideen der Krim (Orchidaceae).- J. Eur. Orch. 46 (2): 407-436. Eine neue nomenklatorische Liste der Orchideen der Krim mit 49 Taxa und 16 Hybriden wird vorgestellt. Sechs Arten sind neu für die Krim. Zehn Taxa, die noch bei YENA (2012) in seiner Checklist aufgelistet wurden, kommen auf der Krim nicht vor und wurden gestrichen. -
Bletilla Striata (Orchidaceae) Seed Coat Restricts the Invasion of Fungal Hyphae at the Initial Stage of Fungal Colonization
plants Article Bletilla striata (Orchidaceae) Seed Coat Restricts the Invasion of Fungal Hyphae at the Initial Stage of Fungal Colonization Chihiro Miura 1, Miharu Saisho 1, Takahiro Yagame 2, Masahide Yamato 3 and Hironori Kaminaka 1,* 1 Faculty of Agriculture, Tottori University, 4-101 Koyama Minami, Tottori 680-8553, Japan 2 Mizuho Kyo-do Museum, 316-5 Komagatafujiyama, Mizuho, Tokyo 190-1202, Japan 3 Faculty of Education, Chiba University, 1-33 Yayoicho, Inage-ku, Chiba 263-8522, Japan * Correspondence: [email protected]; Tel.: +81-857-31-5378 Received: 24 June 2019; Accepted: 8 August 2019; Published: 11 August 2019 Abstract: Orchids produce minute seeds that contain limited or no endosperm, and they must form an association with symbiotic fungi to obtain nutrients during germination and subsequent seedling growth under natural conditions. Orchids need to select an appropriate fungus among diverse soil fungi at the germination stage. However, there is limited understanding of the process by which orchids recruit fungal associates and initiate the symbiotic interaction. This study aimed to better understand this process by focusing on the seed coat, the first point of fungal attachment. Bletilla striata seeds, some with the seed coat removed, were prepared and sown with symbiotic fungi or with pathogenic fungi. The seed coat-stripped seeds inoculated with the symbiotic fungi showed a lower germination rate than the intact seeds, and proliferated fungal hyphae were observed inside and around the stripped seeds. Inoculation with the pathogenic fungi increased the infection rate in the seed coat-stripped seeds. The pathogenic fungal hyphae were arrested at the suspensor side of the intact seeds, whereas the seed coat-stripped seeds were subjected to severe infestation. -
Endophytic Colletotrichum Species from Bletilla Ochracea (Orchidaceae), with Descriptions of Seven New Speices
Fungal Diversity (2013) 61:139–164 DOI 10.1007/s13225-013-0254-5 Endophytic Colletotrichum species from Bletilla ochracea (Orchidaceae), with descriptions of seven new speices Gang Tao & Zuo-Yi Liu & Fang Liu & Ya-Hui Gao & Lei Cai Received: 20 May 2013 /Accepted: 1 July 2013 /Published online: 19 July 2013 # Mushroom Research Foundation 2013 Abstract Thirty-six strains of endophytic Colletotrichum ornamental plants and important research materials for coevo- species were isolated from leaves of Bletilla ochracea Schltr. lution between plants and fungi because of their special sym- (Orchidaceae) collected from 5 sites in Guizhou, China. biosis with mycorrhizal fungi (Zettler et al. 2004; Stark et al. Seventeen different species, including 7 new species (namely 2009; Nontachaiyapoom et al. 2010). Recently, the fungal C. bletillum, C. caudasporum, C. duyunensis, C. endophytum, communities in leaves and roots of orchid Bletilla ochracea C. excelsum-altitudum and C. guizhouensis and C. ochracea), have been investigated and the results indicated that there is a 8 previously described species (C. boninense, C. cereale, C. high diversity of endophytic fungi, including species from the destructivum, C. karstii, C. liriopes, C. miscanthi, C. genus Colletotrichum Corda (Tao et al. 2008, 2012). parsonsiae and C. tofieldiae) and 2 sterile mycelia were iden- Endophytic fungi live asymptomatically and internally with- tified. All of the taxa were identified based on morphology and in different tissues (e.g. leaves, roots) of host plants (Ganley phylogeny inferred from multi-locus sequences, including the and Newcombe 2006; Promputtha et al. 2007; Hoffman and nuclear ribosomal internal transcribed spacer (ITS) region, Arnold 2008). -
Copyrighted Material
Index Page numbers in italics refer to Figures; those in bold to Tables. Abies 32 albuminous cells 42, 44, 65, 65, Acacia alata 81, 85, 98 108 Acer 164 alcian blue 182 Acer pseudoplatanus 165, 166 alcohol-based fi xatives 171–2 achenes 128 aleurone grains 102 acid bog habitat 152 algae 6 Acmopyle pancheri 65 Alismatales 67 acrolein 172–3 Allium 18, 19, 111 fi xation procedure 174–5 Alnus glutinosa 28, 29, 37, 165, 167 adaptations 6–8, 135–53 Alnus nepalensis 29 ecological 73, 76, 137–8 Aloe 9, 76, 77, 78, 139 hydrophytes 150–2 Aloe lateritia var. kitaliensis 77, 79 mechanical 135–7 Aloe somaliensis 140 mesophytes 147–50 aloes 13, 76, 78, 86, 142, 157 practical aspects 152–3 Ammophila 139, 142 xerophytes see xerophytes Ammophila arenaria (marram grass) Aegilops crassa 95, 99, 102 82, 92, 141 aerial roots 49, 149 Anacardiaceae 86, 139 Aerva lanata 81 Anarthria 156 Aesculus hippocastanum 129 Anarthriaceae 156, 156 Aesculus pavia 44 angiosperms 4, 7, 10 Agave 10, 76 fl oral part vascularization 121–3 Agave franzonsinii 95, 102 phloem 65, 108 Agrostis 100, 138COPYRIGHTEDsecondary MATERIAL 43–5 Agrostis stolonifera 99 taxonomy 155 Ailanthus 159 wood (secondary xylem) 31–6, air spaces 36 hydrophytes 150 axial system 33 mesophyll 74, 97, 112 growth rings 33, 35, 41 xerophytes 146 rays 35–6 Ajuga reptans var. atropurpurescens ring porous 33–4, 41 110 animal feeds 159–60 Albuca 73 animal pests 162–3 288 Annonaceae 130 black ironwood (Krugiodendron annuals 7, 8, 57 ferreum) 33 Anthemis 128 Boehmeria 62 Index Anthemis arvenis 128, 130 Bombax (kapok) -
Half Yearly Report.Pdf
National Mission on Himalayan Studies (NMHS) HIMALAYAN RESEARCH FELLOWSHIP (FORMAT FOR THE HALF YEARLY PROGRESS REPORT) [Reporting Period: July to December 2020] Name of the Project: Systematics and Conservation of Indian Orchids with special emphasis to Himalayan species. Sanction Order No.: GBPNI/NMHS-2017-18/HSF-08, dated: 28.03.2018. 1st installment received: Rs. 33,56,232/- Name of the Institution/ University: Botanical Survey of India, Sikkim Himalayan Regional Centre Gangtok, Sikkim No. of Himalayan Research/Project One Associate: No. of Himalayan Junior Research/Project Six (6) Fellows: H-RAs Profile Description: Sl. Name of RA Date of Joining Name of the PI Qualification No. 1A. Dr. SAMIRAN PANDAY 17.12.2018 Dr. D.K. AGRAWALA M.Sc., Ph. D. (BOTANY) (Resigned on - 23.8.2019) 1B. Dr. RIJUPALIKA ROY 14.10.2019 Dr. D.K. AGRAWALA M.Sc., Ph. D. (BOTANY) NMHS Fellowship Grant Page 1 of 13 Progress Report: To be filled for each HRA in separate row. RA No. Research Objectives Achievements Addressed Location of Field Deliverables Site with Details, if any H-RA Project name: Systematics and Conservation of •Morphological Characterization done for •Literature survey • Study area for the 1A. Indian Orchids with special emphasis to 15 species under 8 genera conducted. project is entire Indian Himalayan Himalayan species. •Assigned geo co-ordinates: 23 species •Collection of type, Region covering 12 (425 sheets). protologue & states. Allotted group: Sub-families Vanilloideae, •Literature on selected species has been herbarium Orchidoideae and Epidendroideae with 22 collected and studied. specimen genera and 76 species. •Protologues, types & images of continued. -
Mating Systems in the Pleurothallidinae (Orchidaceae): Evolutionary and Systematic Implications
LANKESTERIANA 11(3): 207—221. 2011. MATING SYSTEMS IN THE PLEUROTHALLIDINAE (ORCHIDACEAE): EVOLUTIONARY AND SYSTEMATIC IMPLICATIONS EDUARDO LEITE BORBA*, ARIANE RAQUEL BARBOSA, MARCOS CABRAL DE MELO, SAMUEL LOUREIRO GONTIJO & HENRIQUE ORNELLAS DE OLIVEIRA Departamento de Botânica, Instituto de Ciências Biológicas Universidade Federal de MinasGerais, Belo Horizonte, MG, 31270-901, Brazil * Corresponding author: [email protected] ABSTRACT. We developed a project addressing the determination of the reproductive system through experimental pollinations of species in the major genera representing all major lineages of Pleurothallidinae in order to determine occurrence of self-incompatibility in the subtribe, in which group it has possibly appeared for the first time, and how many times it has evolved. Additionally we surveyed the floral biology of species ofOctomeria , a genus with morphological characters typical of bee-pollinated flowers that was previously regarded as mellitophilous. At the moment, all but one of the species studied in selected large genera of the major lineages (Acianthera, Anathallis, Masdevallia, Octomeria, Specklinia, and Stelis) are self-incompatible. The species studied may possess complete, strong or partial self-incompatibility. We found two different sites where self-incompatibility reactions occur, the stigma and the stylar channel, and both sites were not found in the same genus except for Anathallis. In Anathallis, the two groups that differ morphologically (formerly Pleurothallis subgen. Specklinia sect. Muscosae -
Systematics and Evolution of the Genus Pleurothallis R. Br
Systematics and evolution of the genus Pleurothallis R. Br. (Orchidaceae) in the Greater Antilles DISSERTATION zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) im Fach Biologie eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät I der Humboldt-Universität zu Berlin von Diplom-Biologe Hagen Stenzel geb. 05.10.1967 in Berlin Präsident der Humboldt-Universität zu Berlin Prof. Dr. J. Mlynek Dekan der Mathematisch-Naturwissenschaftlichen Fakultät I Prof. Dr. M. Linscheid Gutachter/in: 1. Prof. Dr. E. Köhler 2. HD Dr. H. Dietrich 3. Prof. Dr. J. Ackerman Tag der mündlichen Prüfung: 06.02.2004 Pleurothallis obliquipetala Acuña & Schweinf. Für Jakob und Julius, die nichts unversucht ließen, um das Zustandekommen dieser Arbeit zu verhindern. Zusammenfassung Die antillanische Flora ist eine der artenreichsten der Erde. Trotz jahrhundertelanger floristischer Forschung zeigen jüngere Studien, daß der Archipel noch immer weiße Flecken beherbergt. Das trifft besonders auf die Familie der Orchideen zu, deren letzte Bearbeitung für Cuba z.B. mehr als ein halbes Jahrhundert zurückliegt. Die vorliegende Arbeit basiert auf der lang ausstehenden Revision der Orchideengattung Pleurothallis R. Br. für die Flora de Cuba. Mittels weiterer morphologischer, palynologischer, molekulargenetischer, phytogeographischer und ökologischer Untersuchungen auch eines Florenteils der anderen Großen Antillen wird die Genese der antillanischen Pleurothallis-Flora rekonstruiert. Der Archipel umfaßt mehr als 70 Arten dieser Gattung, wobei die Zahlen auf den einzelnen Inseln sehr verschieden sind: Cuba besitzt 39, Jamaica 23, Hispaniola 40 und Puerto Rico 11 Spezies. Das Zentrum der Diversität liegt im montanen Dreieck Ost-Cuba – Jamaica – Hispaniola, einer Region, die 95 % der antillanischen Arten beherbergt, wovon 75% endemisch auf einer der Inseln sind. -
Aphyllorchis Queenslandica Click on Images to Enlarge
Species information Abo ut Reso urces Hom e A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Aphyllorchis queenslandica Click on images to enlarge Family Orchidaceae Scientific Name Aphyllorchis queenslandica Dockrill Dockrill, A.W. (1965) Orchadian 1: 115. Type: Queensland, Helenvale, May 1962, C. Le Roy: Holo: QRS. Common name Herbarium specimen. Copyright CSIRO Yellow Pauper Orchid Stem Above ground part of the plant (peduncle + inflorescence) about 65-90 cm tall. Leaves Plant devoid of chlorophyll. 'Leaves' (bracts) about 8-10 per plant, sessile, about 0.5-3 x 0.6-1.1 cm. Bracts 3-veined, venation longitudinal and parallel. Flowers Sepals about 13 x 2.5 mm. Petals about 11 x 2 mm, labellum larger. Labellum cream, margins upturned, purple. Column purple at the base, but yellow towards the apex. Stamen fused to the style to form a column. Staminal column about 7 x 1.5 mm. Ovary about 12 x 2 mm, outer surface with 6 longitudinal ribs. Fruit Inflorescence about 40-70 cm long, usually 6-12-flowered. Peduncle with up to 10 stem-clasping bracts, each bract about 5-20 x 6-10 mm. Bracts subtending the flowers about 10 x 2 mm and are not stem-clasping. Flowers about 20 mm wide on pedicels about 2-3 mm long. Sepals about 11-13 c 3-4 mm. Petals about 11 x 3 mm. Labellum about 6 x 2.5 mm. Column about 6-8 x 1.5 mm, curved, semi-cylindrical. -
Orchid Historical Biogeography, Diversification, Antarctica and The
Journal of Biogeography (J. Biogeogr.) (2016) ORIGINAL Orchid historical biogeography, ARTICLE diversification, Antarctica and the paradox of orchid dispersal Thomas J. Givnish1*, Daniel Spalink1, Mercedes Ames1, Stephanie P. Lyon1, Steven J. Hunter1, Alejandro Zuluaga1,2, Alfonso Doucette1, Giovanny Giraldo Caro1, James McDaniel1, Mark A. Clements3, Mary T. K. Arroyo4, Lorena Endara5, Ricardo Kriebel1, Norris H. Williams5 and Kenneth M. Cameron1 1Department of Botany, University of ABSTRACT Wisconsin-Madison, Madison, WI 53706, Aim Orchidaceae is the most species-rich angiosperm family and has one of USA, 2Departamento de Biologıa, the broadest distributions. Until now, the lack of a well-resolved phylogeny has Universidad del Valle, Cali, Colombia, 3Centre for Australian National Biodiversity prevented analyses of orchid historical biogeography. In this study, we use such Research, Canberra, ACT 2601, Australia, a phylogeny to estimate the geographical spread of orchids, evaluate the impor- 4Institute of Ecology and Biodiversity, tance of different regions in their diversification and assess the role of long-dis- Facultad de Ciencias, Universidad de Chile, tance dispersal (LDD) in generating orchid diversity. 5 Santiago, Chile, Department of Biology, Location Global. University of Florida, Gainesville, FL 32611, USA Methods Analyses use a phylogeny including species representing all five orchid subfamilies and almost all tribes and subtribes, calibrated against 17 angiosperm fossils. We estimated historical biogeography and assessed the -
Redalyc.ARE OUR ORCHIDS SAFE DOWN UNDER?
Lankesteriana International Journal on Orchidology ISSN: 1409-3871 [email protected] Universidad de Costa Rica Costa Rica BACKHOUSE, GARY N. ARE OUR ORCHIDS SAFE DOWN UNDER? A NATIONAL ASSESSMENT OF THREATENED ORCHIDS IN AUSTRALIA Lankesteriana International Journal on Orchidology, vol. 7, núm. 1-2, marzo, 2007, pp. 28- 43 Universidad de Costa Rica Cartago, Costa Rica Available in: http://www.redalyc.org/articulo.oa?id=44339813005 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative LANKESTERIANA 7(1-2): 28-43. 2007. ARE OUR ORCHIDS SAFE DOWN UNDER? A NATIONAL ASSESSMENT OF THREATENED ORCHIDS IN AUSTRALIA GARY N. BACKHOUSE Biodiversity and Ecosystem Services Division, Department of Sustainability and Environment 8 Nicholson Street, East Melbourne, Victoria 3002 Australia [email protected] KEY WORDS:threatened orchids Australia conservation status Introduction Many orchid species are included in this list. This paper examines the listing process for threatened Australia has about 1700 species of orchids, com- orchids in Australia, compares regional and national prising about 1300 named species in about 190 gen- lists of threatened orchids, and provides recommen- era, plus at least 400 undescribed species (Jones dations for improving the process of listing regionally 2006, pers. comm.). About 1400 species (82%) are and nationally threatened orchids. geophytes, almost all deciduous, seasonal species, while 300 species (18%) are evergreen epiphytes Methods and/or lithophytes. At least 95% of this orchid flora is endemic to Australia. -
Phylogeny, Character Evolution and the Systematics of Psilochilus (Triphoreae)
THE PRIMITIVE EPIDENDROIDEAE (ORCHIDACEAE): PHYLOGENY, CHARACTER EVOLUTION AND THE SYSTEMATICS OF PSILOCHILUS (TRIPHOREAE) A Dissertation Presented in Partial Fulfillment of the Requirements for The Degree Doctor of Philosophy in the Graduate School of the Ohio State University By Erik Paul Rothacker, M.Sc. ***** The Ohio State University 2007 Doctoral Dissertation Committee: Approved by Dr. John V. Freudenstein, Adviser Dr. John Wenzel ________________________________ Dr. Andrea Wolfe Adviser Evolution, Ecology and Organismal Biology Graduate Program COPYRIGHT ERIK PAUL ROTHACKER 2007 ABSTRACT Considering the significance of the basal Epidendroideae in understanding patterns of morphological evolution within the subfamily, it is surprising that no fully resolved hypothesis of historical relationships has been presented for these orchids. This is the first study to improve both taxon and character sampling. The phylogenetic study of the basal Epidendroideae consisted of two components, molecular and morphological. A molecular phylogeny using three loci representing each of the plant genomes including gap characters is presented for the basal Epidendroideae. Here we find Neottieae sister to Palmorchis at the base of the Epidendroideae, followed by Triphoreae. Tropidieae and Sobralieae form a clade, however the relationship between these, Nervilieae and the advanced Epidendroids has not been resolved. A morphological matrix of 40 taxa and 30 characters was constructed and a phylogenetic analysis was performed. The results support many of the traditional views of tribal composition, but do not fully resolve relationships among many of the tribes. A robust hypothesis of relationships is presented based on the results of a total evidence analysis using three molecular loci, gap characters and morphology. Palmorchis is placed at the base of the tree, sister to Neottieae, followed successively by Triphoreae sister to Epipogium, then Sobralieae.