DOCSLIB.ORG

Some Reproductive Aspects of the Indian Squid Loligo Duvauceli in the Gulf of Suez, Egypt

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Some Reproductive Aspects of the Indian Squid Loligo Duvauceli in the Gulf of Suez, Egypt Egyptian Journal of Aquatic Biology & Fisheries Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt. ISSN 1110 – 6131 Vol. 25(3): 367 – 382 (2021) www.ejabf.journals.ekb.eg Some reproductive aspects of the Indian squid Loligo duvauceli in the Gulf of Suez, Egypt. Ahmed G.A. Gewida1,*; Mohamed H. Yassien2; Mohsen S. Hussein1 and Ahmed Mamoon1 1 Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo 11884, Egypt. 2 National Institute of Oceanography and Fisheries, Suez Branch, Egypt. *Corresponding author: [email protected] ARTICLE INFO ABSTRACT Article History: A better knowledge of the reproductive biology of Loligo Received: Jan. 30, 2021 duvauceli squid, such as its sex ratio, sexual maturation, and reproductive Accepted: April 30, 2021 seasonality is fundamental for establishing effective fishery management Online: May 30, 2021 measures to ensure the fishery is sustainable. Samples of Loligo _______________ duvauceli were collected monthly for one fishing season during September 2014 to April 2015 at Attaka fishing harbor in the Gulf of Suez. A total of Keywords: 759 individuals (434 males, 268 females, and 57 not recognized) in size Maturity stage, range of 3.5 to 29.5 cm dorsal mantle length (DML) were used for this Reproductive biology, study. The sex ratio of males: females was 1.6:1.0. Females outnumbered Sex ratio, males only in February. However, males were dominant in the rest of the Spawning season. fishing season. Four maturity stages for both sexes were recognized. Monthly variations in maturity stages and maturity indices showed that the spawning season extends from April to October (spring to early autumn) for females, while males from April to September (spring to late autumn). Length at first sexual maturity indicated that the females attain maturity at smaller DML than males. The mean length at maturity for this species was 14.8 and 17.5cm DML for females and males, respectively. INTRODUCTION The Gulf of Suez is considered as one of the major sources of fish production in the Egyptian sector of the Red Sea. Its importance as fish resource can be attributed to the shallowness and sandy bottom which make it suitable for trawling. It is characterized by the presence of a great diversity of highly economic fish and invertebrate species (Sanders and Morgan, 1989). The increasing exploitation of finfish resources and the depletion of a number of major fish stocks that formerly supported industrial-scale fisheries, forces continued attention to the once-called ‘unconventional marine resources’, which include numerous species of cephalopods (Jereb and Roper, 2010). All cephalopods are dioecious. Sexual maturation involves the onset of the development of sexual organs from immature to mature individuals. Typically, a 868 Gewida et al., 2021 certain minimum size must be reached before maturation can proceed (Jackson et al., 1997). All loliginid squids have a strong preference for the benthic environment for feeding and spawning (Boyle & Rodhouse, 2005 and Yassien et al., 2016). They are all demersal spawners, where the egg capsules are attached to any natural or artificial surfaces such as rocks, macroalgae, fishing traps etc. Some species, such as Loligo vulgaris, directly insert their egg masses into sandy sea floors (Sauer et al., 1993). Loligo duvauceli is one of the loliginid species that is abundant in the Egyptian Red Sea coast and the Gulf of Suez (Riad, 2020). Along these coasts, the commercial catch of L. duvauceli is mainly landed as by-catch of multispecies demersal trawling fisheries (Yassien et al., 2016). Previous reproductive studies on Loligo duvauceli have been done in different regions such as India: (Mohamed, 1993; Thomas & Kizhakudan, 2006; Mishra et al., 2012), Thailand: (Supongpan et al., 1992; Chotiyaputta, 1996; Sukramongkol et al., 2007) and China: (Sang, 2007; Wang et al., 2015). Recently, few studies have been done in Egypt: Kilada & Riad (2010) who inspected the seasonal reproduction biology of Uroteuthis duvaucili in the northern Red Sea. Mohamed (2013) studied maturation, fecundity and seasonality reproduction of U. duvaucili in the Suez Canal. The main objective of the present study is to shed light on the reproductive aspects of L. duvauceli in the Gulf of Suez, including sex ratio, maturity stages and indices of reproductive parameters in addition to determining of the spawning season of this species. MATERIALS AND METHODS Sampling Monthly sampling was done throughout the period of the study during one fishing season (from September 2014 to April 2015). A total of 759 samples (434 males, 268 females and 57 not recognized) ranged in size from 3.5 to 29.5 cm DML and 3.2 to 224.1g were collected from commercial stern trawlers at Attaka fishing port of the Gulf of Suez. The squid specimens were packed into an insulated icebox to keep them fresh during transporting them to the laboratory for identification, sex determination and subsequent biological analysis on reproductive organs. Sex ratio Each individual was dissected by cutting along the ventral mantle to reveal the internal organs and sex was determined by assessing its sexual organs visually. As for some smaller individuals that could not be distinguished by sexual characteristics, they were then assigned as ‘not recognized’ where no maturity stage was applied. Monthly sex ratios were calculated to determine the variations in sex ratios on a monthly basis. The following measurements were recorded to the nearest 0.01g using an electronic balance: Reproductive aspects of the Indian squid in the Gulf of Suez. 369 ______________________________________________________________________________ Spermatophoric organs weight (SOW): Weight of the spermatophoric complex and spermatophoric sac in males. Testis weight (TW): Weight of the testis in g. Nidamental glands weight (NGW): Weight of the nidamental glands and its accessory glands in females. Oviductal gland weight (OGW): Weight of the oviductal gland in g. Ovary weight (OW): Weight of the ovary in g. Maturity stages A macroscopic visual maturity stage scale (After Lipinski and Underhill, 1995 with some modifications) was used to assess the gonads and their development. Four stages are defined for both males and females, stage I (immature), stage II (maturing), stage III (mature) and stage IV (spawning) as shown in figure (1). For males, stages were defined based on the development of the spermatophoric complex, development of spermatophoric sac, size and appearance of the testis, and the presence or absence of spermatophores (the sperm packets). For females, the color of the accessory nidamental glands, size of the nidamental glands and ovary, and the presence or absence of fully mature oocytes in the proximal oviduct was used for the assessments (Figure 1). Monthly variations in maturity stages Monthly variations in maturity stages were used to determine the spawning season and the occurrence of mature males and females of L. duvauceli squid in the Gulf of Suez. Length at first sexual maturity (Lm) The length at first sexual maturity (Lm); length at which 50% of squid reach their sexual maturity (Bakhayokho, 1983) was estimated by fitting the percentage of maturity against mid lengths. Lm was estimated as the point on X-axis corresponding to 50% point on Y-axis. Indices of reproductive parameters The reproductive parameters indices were calculated as follows: For females Gonadosomatic index (GSI) = 100 * OW / BW Nidamental gland index (NGI) = 100 * NGW/ BW Oviductal gland index (OGI) = 100 * OGW / BW Maturity coefficient (MC) = 100 * RSW / BW Where: BW = Total body weight, OW = Ovary weight, NGW =Nidamental gland weight, OGW = Oviductal gland weight, RSW = Reproductive system weight (NGW + OGW + OW) 873 Gewida et al., 2021 For males Gonadosomatic index (GSI) = 100 * TW / BW Spermatophoric organs index (SOI) = 100 * SOW / BW Maturity coefficient (MC) = 100 * RSW / BW Where: BW = Total body weight, TW = Testis weight, SOW = Spermatophoric organs (complex & Sac) weight, RSW = Reproductive system weight (SOW + TW) Figure 1. Different visual maturity stages of (a) male and (b) female of L. duvauceli (I): immature, (II): maturing, (III): mature, (IV): spawning. (After Lipinski and Underhill 1995) Reproductive aspects of the Indian squid in the Gulf of Suez. 371 ______________________________________________________________________________ RESULTS Sex ratio In total sample (702 specimens) of L. duvauceli the sex ratio (M: F) was 1.6:1.0. Males resembled 61.82%, while female formed 38.18% of the examined samples during the study period. Females outnumbered males only in February; however, males were dominant in the rest of the months (Figure 2). As to the seasonal variation of sex ratio in the present study, it was found that males were predominant over females in all seasons (Figure 3). Figure 2. Monthly variations in sex ratio of L. duvauceli collected from the Gulf of Suez. Figure 3. Seasonal variations in sex ratio of L. duvauceli from the Gulf of Suez. The sex ratio in relation to dorsal mantle length (DML) classes for 759 specimens of L. duvauceli (Figure 4). The not recognized specimens (7.51%) were smaller in size and less than 11.5 cm DML and a presence of female (35.31%) was 873 Gewida et al., 2021 noticed for individuals from 7.5 to 21.5 cm DML, while males (57.18%) clearly dominated from 7.5 cm DML onwards. Figure 4. Sex ratio for L. duvauceli in relation to dorsal mantle length from the Gulf of Suez. Maturity stages Four maturity stages (according to Lipinski and Underhill (1995) with some modifications) for both males and females were recorded throughout the sampling period and classified as follows: Stage I (immature) For males: Spermatophoric complex is visible and testes are small, usually milky white. For females: Nidamental glands are easily visible and ovary does not preserve well. Stage II (maturing) For males: Separate parts of the spermatophoric complex are visible. Testes are small and without its normal structure. For females: The nidamental glands do not obscure the underlying viscera, semi-translucent and white.
Load more

Recommended publications
  • Environmental Effects on Cephalopod Population Dynamics: Implications for Management of Fisheries
    Advances in Cephalopod Science:Biology, Ecology, Cultivation and Fisheries,Vol 67 (2014) Provided for non-commercial research and educational use only. Not for reproduction, distribution or commercial use. This chapter was originally published in the book Advances in Marine Biology, Vol. 67 published by Elsevier, and the attached copy is provided by Elsevier for the author's benefit and for the benefit of the author's institution, for non-commercial research and educational use including without limitation use in instruction at your institution, sending it to specific colleagues who know you, and providing a copy to your institution’s administrator. All other uses, reproduction and distribution, including without limitation commercial reprints, selling or licensing copies or access, or posting on open internet sites, your personal or institution’s website or repository, are prohibited. For exceptions, permission may be sought for such use through Elsevier's permissions site at: http://www.elsevier.com/locate/permissionusematerial From: Paul G.K. Rodhouse, Graham J. Pierce, Owen C. Nichols, Warwick H.H. Sauer, Alexander I. Arkhipkin, Vladimir V. Laptikhovsky, Marek R. Lipiński, Jorge E. Ramos, Michaël Gras, Hideaki Kidokoro, Kazuhiro Sadayasu, João Pereira, Evgenia Lefkaditou, Cristina Pita, Maria Gasalla, Manuel Haimovici, Mitsuo Sakai and Nicola Downey. Environmental Effects on Cephalopod Population Dynamics: Implications for Management of Fisheries. In Erica A.G. Vidal, editor: Advances in Marine Biology, Vol. 67, Oxford: United Kingdom, 2014, pp. 99-233. ISBN: 978-0-12-800287-2 © Copyright 2014 Elsevier Ltd. Academic Press Advances in CephalopodAuthor's Science:Biology, personal Ecology, copy Cultivation and Fisheries,Vol 67 (2014) CHAPTER TWO Environmental Effects on Cephalopod Population Dynamics: Implications for Management of Fisheries Paul G.K.
    [Show full text]
  • Geographic Drivers of Diversification in Loliginid Squids with an Emphasis on the Western Atlantic Species
    bioRxiv preprint doi: https://doi.org/10.1101/2020.07.20.211896; this version posted July 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Original Article Geographic drivers of diversification in loliginid squids with an emphasis on the western Atlantic species Gabrielle Genty1*, Carlos J Pardo-De la Hoz1,2*, Paola Montoya1,3, Elena A. Ritschard1,4* 1Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá D.C, Colombia. 2Department of Biology, Duke University, Durham, North Carolina, 27708, United States of America 3Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, Bogotá, D.C., Colombia 4Department of Neuroscience and Developmental Biology, University of Vienna, Austria * These authors contributed equally to this work. Correspondence author: Gabrielle Genty, [email protected] Acknowledgements We would like to thank Daniel Cadena and Andrew J. Crawford for their suggestions and guidance during the early stages of this investigation. bioRxiv preprint doi: https://doi.org/10.1101/2020.07.20.211896; this version posted July 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 2 ABSTRACT Aim: Identifying the mechanisms driving divergence in marine organisms is challenging as opportunities for allopatric isolation are less conspicuous than in terrestrial ecosystems.
    [Show full text]
  • Cephalopod Reproductive Strategies Derived from Embryonic Shell Size
    Biol. Rev. (2017), pp. 000–000. 1 doi: 10.1111/brv.12341 Cephalopod embryonic shells as a tool to reconstruct reproductive strategies in extinct taxa Vladimir Laptikhovsky1,∗, Svetlana Nikolaeva2,3,4 and Mikhail Rogov5 1Fisheries Division, Cefas, Lowestoft, NR33 0HT, U.K. 2Department of Earth Sciences Natural History Museum, London, SW7 5BD, U.K. 3Laboratory of Molluscs Borissiak Paleontological Institute, Russian Academy of Sciences, Moscow, 117997, Russia 4Laboratory of Stratigraphy of Oil and Gas Bearing Reservoirs Kazan Federal University, Kazan, 420000, Russia 5Department of Stratigraphy Geological Institute, Russian Academy of Sciences, Moscow, 119017, Russia ABSTRACT An exhaustive study of existing data on the relationship between egg size and maximum size of embryonic shells in 42 species of extant cephalopods demonstrated that these values are approximately equal regardless of taxonomy and shell morphology. Egg size is also approximately equal to mantle length of hatchlings in 45 cephalopod species with rudimentary shells. Paired data on the size of the initial chamber versus embryonic shell in 235 species of Ammonoidea, 46 Bactritida, 13 Nautilida, 22 Orthocerida, 8 Tarphycerida, 4 Oncocerida, 1 Belemnoidea, 4 Sepiida and 1 Spirulida demonstrated that, although there is a positive relationship between these parameters in some taxa, initial chamber size cannot be used to predict egg size in extinct cephalopods; the size of the embryonic shell may be more appropriate for this task. The evolution of reproductive strategies in cephalopods in the geological past was marked by an increasing significance of small-egged taxa, as is also seen in simultaneously evolving fish taxa. Key words: embryonic shell, initial chamber, hatchling, egg size, Cephalopoda, Ammonoidea, reproductive strategy, Nautilida, Coleoidea.
    [Show full text]
  • The Bigfin Reef Squid)
    Southern Illinois University Carbondale OpenSIUC Honors Theses University Honors Program 5-11-2013 Phylogeography of Sepioteuthis lessoniana (the bigfin eefr squid) and Uroteuthis duvauceli (the Indian squid). Alexis M. Bergman Southern Illinois University Carbondale, [email protected] Follow this and additional works at: http://opensiuc.lib.siu.edu/uhp_theses Recommended Citation Bergman, Alexis M., "Phylogeography of Sepioteuthis lessoniana (the bigfin er ef squid) and Uroteuthis duvauceli (the Indian squid)." (2013). Honors Theses. Paper 353. This Dissertation/Thesis is brought to you for free and open access by the University Honors Program at OpenSIUC. It has been accepted for inclusion in Honors Theses by an authorized administrator of OpenSIUC. For more information, please contact [email protected]. Phylogeography of Sepioteuthis lessoniana (the bigfin reef squid) and Uroteuthis duvauceli (the Indian squid). Alexis Bergman A thesis submitted to the University Honors Program in partial fulfillment of the requirements for the Honors Degree Southern Illinois University April 18, 2013 2 Abstract Sepioteuthis lessoniana (the bigfin reef squid) and Uroteuthis duvauceli (the Indian squid) are two squid species found in largely overlapping regions in the Indian and Pacific Oceans. While both squids are important to fisheries throughout their ranges, very little taxonomic work has been done on either of them. Previous studies have led scientists to believe that S. lessoniana is actually a species complex (for example, there appear to be three species of “S. cf. lessoniana” in Japanese waters alone). The similarly broad geographic range of U. duvauceli suggests that this species could also harbor substantial cryptic genetic diversity. In order to evaluate genetic variation within these two species, regions of two mitochondrial genes—the large subunit ribosomal RNA gene (16S) and the cytochrome oxidase I gene (COI)—from specimens caught in regions throughout the northern Indian and western Pacific Oceans were sequenced and compared.
    [Show full text]
  • Reviews in Fisheries Science & Aquaculture World Squid Fisheries
    This article was downloaded by: [British Antarctic Survey] On: 13 July 2015, At: 06:37 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: 5 Howick Place, London, SW1P 1WG Reviews in Fisheries Science & Aquaculture Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/brfs21 World Squid Fisheries Alexander I. Arkhipkina, Paul G. K. Rodhouseb, Graham J. Piercecd, Warwick Sauere, Mitsuo Sakaif, Louise Allcockg, Juan Arguellesh, John R. Boweri, Gladis Castilloh, Luca Ceriolaj, Chih-Shin Chenk, Xinjun Chenl, Mariana Diaz-Santanam, Nicola Downeye, Angel F. Gonzálezn, Jasmin Granados Amoreso, Corey P. Greenp, Angel Guerran, Lisa C. Hendricksonq, Christian Ibáñezr, Kingo Itos, Patrizia Jerebt, Yoshiki Katof, Oleg N. Katuginu, Mitsuhisa Kawanov, Hideaki Kidokorow, Vladimir V. Kuliku, Vladimir V. Laptikhovskyx, Marek R. Lipinskid, Bilin Liul, Luis Mariáteguih, Wilbert Marinh, Ana Medinah, Katsuhiro Mikiy, Kazutaka Miyaharaz, Natalie Moltschaniwskyjaa, Hassan Moustahfidab, Jaruwat Nabhitabhataac, Nobuaki Nanjoad, Chingis M. Nigmatullinae, Tetsuya Ohtaniaf, Gretta Peclag, J. Angel A. Perezah, Uwe Click for updates Piatkowskiai, Pirochana Saikliangaj, Cesar A. Salinas-Zavalao, Michael Steerak, Yongjun Tianw, Yukio Uetaal, Dharmamony Vijaiam, Toshie Wakabayashian, Tadanori Yamaguchiao, Carmen Yamashiroh, Norio Yamashitaap & Louis D. Zeidbergaq a Fisheries Department, Stanley, Falkland Islands b British Antarctic Survey, Natural
    [Show full text]
  • Age, Growth and Mortality Studies of Indian Squid, Uroteuthis (Photololigo) Duvauceli (D’ Orbigny) Along Ratnagiri Coast of Maharashtra, India
    Indian Journal of Geo-Marine Sciences Vol. 44(1), January 2015, pp. 93-96 Age, growth and mortality studies of Indian squid, Uroteuthis (Photololigo) duvauceli (d’ Orbigny) along Ratnagiri Coast of Maharashtra, India Pawar Nitin, Nirmale V.H., Metar S.Y., Bhosale, B.P. , Sawant, M.S & Naik, S.D. College of Fisheries, Ratnagiri-415629, Maharashtra, India *[E-mail: [email protected] ] Received 18 November 2013; revised 28 January 2014 The study on the growth and mortality of Indian squid Uroteuthis duvauceli was carried out based on the length frequency data collected during February 2012 to January 2013. Using ELEFAN, the asymptotic length ((L∞)) and growth coefficient (K) were estimated as 376 mm and 0.95 yr-1 respectively. Using the von Bertalanffy’s Growth Formula (VBGF), it is seen that L. duvauceli attain 233, 324 and 355 mm at the end of Ist – IIIrd years respectively. The total, natural and fishing -1 mortalities were 4.5, 1.82 and 2.68 yr respectively. The Lc50 was found to be 107 mm. The exploitation rate (U) and exploitation ratio (E) was calculated 0.53 and 0.54 respectively. The value of E max in the present study is lower than exploitation ratio E of 0.54.This indicates that to maximize the yield per recruit from L. duvauceli the efforts may be reduced from the present E of 0.54 to 0 .5 to sustain the stock. Key words: Indian squid, Uroteuthis duvauceli, growth, mortality, stock assessment conducted on growth, mortality and stock Introduction assessment of this species. The Indian squid Uroteuthis duvauceli .
    [Show full text]
  • New Molecular Phylogeny of the Squids of the Family
    Molecular Phylogenetics and Evolution 68 (2013) 293–299 Contents lists available at SciVerse ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev New molecular phylogeny of the squids of the family Loliginidae with emphasis on the genus Doryteuthis Naef, 1912: Mitochondrial and nuclear sequences indicate the presence of cryptic species in the southern Atlantic Ocean ⇑ João Bráullio de Luna Sales a, , Paul W. Shaw b, Manuel Haimovici c, Unai Markaida d, Divino B. Cunha a, Jonathan Ready a, Wilsea M.B. Figueiredo-Ready a, Horacio Schneider a, Iracilda Sampaio a a Universidade Federal do Pará, Campus Universitário de Bragança, Laboratório de Genética e Biologia Molecular, Bragança/PA CEP 68600-000, Brazil b Institute of Biological, Environmental and Rural Science (IBERS), Aberystwyth University, Penglais, Aberystwyth SY23 3DA, UK c Universidade Federal do Rio Grande (FURG), Laboratório de Recursos Demersais e Cefalópodes, Caixa Postal 474, Rio Grande/RS CEP 96201-900, Brazil d Laboratorio de Pesquerías Artesanales, El Colegio de La Fronteira Sur, Unidad Campeche, 24500 Campeche, Mexico article info abstract Article history: The family Loliginidae Lesueur, 1821, is currently considered to include seven genera and approximately Received 6 December 2012 50 species of neritic and coastal squids. These commercially important species occur in tropical and tem- Revised 23 March 2013 perate coastal waters around the world. The taxonomy of the family has been revised a number of times Accepted 25 March 2013 in recent years, focusing in particular on genera such as Doryteuthis, Sepioteuthis, Alloteuthis, and Uroteu- Available online 6 April 2013 this, which are represented by populations in the New World, Oceania, Europe/Africa, and Asia.
    [Show full text]
  • Download (5MB)
    Mar. Fish. Infor. Serv., T & E Ser., No. 231, 2017 1 From the Editorial Board……. Warm greetings to all he effects of global warming and Climate Change happening around Tthe world is becoming evident each passing day. The warmer sea surface temperatures triggering coral bleaching eventually leading to their death and affecting the marine ecosystem adversely, has been reported from several places globally. A case study on the coral bleaching phenomenon in the Palk Bay is presented in this compilation of MFIS. Innovations in seaweed farming integrated with fish farming in cages, trade in marine ornamental gastropods, emerging fishery resources, records of marine biodiversity and fish seed prospecting to augment supply of fish seeds for aquaculture activities are also reported. Marine Fisheries Information Service Marine Fisheries 2 Mar. Fish. Infor. Serv., T & E Ser., No. 231, 2017 Marine Fisheries Information Service No. 231, January-March 2017 Abbreviation - Mar. Fish. Infor. Serv., T & E Ser. CONTENTS Coral bleaching: causes, consequences and mitigation 3 Experimental cultivation of seaweed Kappaphycus alvarezii using net-tube method 9 Ornamental gastropod shell trade in India : A macroeconomic assessment 11 Marine cage farming of Asian seabass under participatory mode - A success story 13 Large scale farming of green mussel in Ashtamudi Lake, Kerala 15 Standardization of culture aspects of Apocyclops cmfri sp. nov. a native cyclopoid copepod 17 A note on the button shells fishery in Kakinada Bay 19 Distribution of Plesionika semilaevis
    [Show full text]
  • Molecular Identification and Genetic Variation Studies in Economically Important Cephalopods at Beypore Fishing Harbour (Kozhikode), South West Coast of India
    Lincy A et al . (2021) Notulae Scientia Biologicae 13(1):10862 DOI:10.15835/nsb13110862 AcademicPres Research Article Notulae Scientia Biologicae Molecular identification and genetic variation studies in economically important cephalopods at Beypore Fishing Harbour (Kozhikode), South West coast of India Alex LINCY 1*, Madhavankonath K. ANIL 2, Muthusamy THANGARAJ 3, Jean J. JOSE 1 1University of Kerala, St. Gregorios College, Department of Zoology, Kottarakara-691531, India; [email protected] (*corresponding author); [email protected] 2Central Marine Fisheries Research Institute, Regional Centre, Vizhinjam-695521, Kerala, India; [email protected] 3Annamalai University, Faculty of Marine Sciences, Centre of Advanced Study in Marine Biology, Parangipettai-608502, Tamil Nadu, India; [email protected] Abstract Cephalopods are ecologically and economically important marine groups in the world. Biodiversity description is essential for sustainable utilization of natural resources and to characterize biological entities for conservation. DNA barcoding is an effective tool used for identification of organisms at species level and is been widely used for delineate several ambiguity species. In this study, partial sequence of mitochondrial cytochrome c oxidase 1 (CO1) gene with a mean size of 680 bp was amplified by universal primers. Totally 13 individuals of Cephalopods comprising of three species, were barcoded and genetic variation was analysed. The maximum A+T content (67.60%) was recorded in Cistopus indicus and minimum (63.70%) in Sepioteuthis lessoniana . The maximum K2P distance (0.268) was found between the genus Cistopus and Sepioteuthis whereas the minimum distance (0.188) was observed between Uroteuthis and Sepia. The neighbour joining tree revealed three distinct clades represents Loligonidae, Sepiidae and Octopodidae with high boot strap values.
    [Show full text]
  • United States National Museum Bulletin 234
    i's {(mi lw|f SMITHSONIAN INSTITUTION UNITED STATES NATIONAL MUSEUM BULLETIN 234 WASHINGTON, D.C. 1963 M USEUM OF NATURAL HIS t]o R Y Cephalopods of the Philippine Islands GILBERT L. VOSS SMITHSONIAN INSTITUTION WASHINGTON, 1963 Publications of the United States National Museum The scientific publications of the United States National Museum include two series, Proceedings of the United States National Museum and United States National Museum Bulletin. In these series are published original articles and monogi-aphs dealing with the collections and work of the Museum and setting forth newly acquired facts in the fields of Anthropology, Biology, Geology, History, and Technology. Copies of each publication are distributed to libraries and scientific organizations and to specialists and others interested in the different subjects. The Proceedings, begun in 1878, are intended for the publication, in separate form, of shorter papers. These are gathered in volumes, octavo in size, with the publication of each paper recorded in the table of contents of the volume. In the Bulletin series, the first of which was issued in 1875, appear longer, separate publications consisting of monographs (occasionally in several parts) and volumes in which are collected works on related subjects. Bulletins are either octavo or quarto in size, depending on the needs of the presentation. Since 1902 papers relating to the botanical collections of the Museum have been published in the Bulletin series under the heading Contributions from the United States National Eerbarium. This work forms number 234 of the Bulletin series. Frank A. Tayloe, Director, United States National Museum. /^HSO,^ U.S* GOVERNMENT PRINTING OFFICE WASHINGTON i 1963 For sale by the Superintendent of Documents, U.S.
    [Show full text]
  • Science Journals — AAAS
    SCIENCE ADVANCES | RESEARCH ARTICLE ECOLOGY Copyright © 2021 The Authors, some rights reserved; Offshore pelagic subsidies dominate carbon inputs exclusive licensee American Association to coral reef predators for the Advancement C. Skinner1,2*, A. C. Mill1, M. D. Fox3, S. P. Newman1,4, Y. Zhu1, A. Kuhl5, N. V. C. Polunin1 of Science. No claim to original U.S. Government Works. Distributed Coral reefs were traditionally perceived as productive hot spots in oligotrophic waters. While modern evidence under a Creative indicates that many coral reef food webs are heavily subsidized by planktonic production, the pathways through Commons Attribution which this occurs remain unresolved. We used the analytical power of carbon isotope analysis of essential amino License 4.0 (CC BY). acids to distinguish between alternative carbon pathways supporting four key reef predators across an oceanic atoll. This technique separates benthic versus planktonic inputs, further identifying two distinct planktonic pathways (nearshore reef-associated plankton and offshore pelagic plankton), and revealing that these reef predators are overwhelmingly sustained by offshore pelagic sources rather than by reef sources (including reef-associated plankton). Notably, pelagic reliance did not vary between species or reef habitats, emphasizing that allochthonous energetic subsidies may have system-wide importance. These results help explain how coral reefs maintain excep- tional productivity in apparently nutrient-poor tropical settings, but also emphasize their susceptibility to future ocean productivity fluctuations. Downloaded from INTRODUCTION evidence to determine the actual origins of pelagic inputs to coral Ecosystem function relies on the movement and storage of energy reefs. However, as climate change is predicted to cause declines in and nutrients (1).
    [Show full text]
  • Extraction and Purification of Collagen from Marine Squid Uroteuthis Duvauceli
    ijlpr 2020; doi 10.22376/ijpbs/lpr.2020.10.4.L77-89 International Journal of Life science and Pharma Research Biotechnology for prospective Medical Science Research Article Extraction and Purification of Collagen from Marine Squid Uroteuthis Duvauceli Jency George1* and Dr. Manjusha W. A.2 1*Research Scholar, Register no: 18113082022004, Interdisciplinary Research Centre, Department of Biotechnology, Malankara Catholic College, Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli - 627 012, Tamil Nadu, India. 2Head and Assistant Professor of Biotechnology Department, Malankara Catholic College, MS University, Tirunelveli, India Abstract: Collagen is a protein, which plays a significant role in the development of tissues and organs. These are used for cosmetic surgery and as wound healing aids in burn patients, remaking of bone and a wide assortment of dental, orthopedic and surgical purposes. These days’ collagen is isolated from calf, porcine tissue and so forth. But it can cause the onset of diseases such as, for example, Bovine spongiform encephalopathy (BSE), Transmissible spongiform encephalopathy (TSE), Foot-and-mouth illness (FMD), and so forth and furthermore cultural and social concerns. As another option, collagen can be separated from marine squid. The aim of the present work is to isolate and partially characterize the collagen from the marine squid, Uroteuthis duvauceli. The confined collagen was evaluated by utilizing Bradford test and partially purified by using gel filtration on a Sepharose 4B column. These filtered collagens were affirmed by SDS PAGE. These days industrially accessible collagen in the market are costly and are not affordable to the common people. The present data on collagens from the marine squid, could help the future endeavors to unwind the therapeutically significant, more secure collagens from marine squid for their utilization in the biomedical field.
    [Show full text]