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Anti-WDR37 Antibody, Mouse Monoclonal Clone WDR37-9, Purified from Hybridoma Cell Culture Catalog Number SAB4200464 Product

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Anti-WDR37 Antibody, Mouse Monoclonal Clone WDR37-9, Purified from Hybridoma Cell Culture Catalog Number SAB4200464 Product Anti-WDR37 antibody, Mouse monoclonal clone WDR37-9, purified from hybridoma cell culture Catalog Number SAB4200464 Product Description Reagent Anti-WDR37 (mouse IgG1 isotype) is derived from the Supplied as a solution in 0.01 M phosphate buffered hybridoma WDR37-9 produced by the fusion of mouse saline, pH 7.4, containing 15 mM sodium azide as a myeloma cells and splenocytes from BALB/c mice preservative. immunized with a synthetic peptide corresponding to a sequence at the N-terminus of human WDR37 Antibody Concentration: ~ 1.0 mg/mL (GeneID: 22884), conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Precautions and Disclaimer Antibody Isotyping Reagents, Catalog Number ISO2. For R&D use only. Not for drug, household, or other The antibody is purified from culture supernatant of uses. Please consult the Material Safety Data Sheet hybridoma cells grown in a bioreactor. for information regarding hazards and safe handling practices. Anti-WDR37 recognizes human, bovine, dog and mouse WDR37. The product may be used in several Storage/Stability immunochemical techniques including immunoblotting For extended storage, freeze at −20 °C in working (~ 55 kDa), immunocytochemistry and aliquots. Repeated freezing and thawing, or storage in immunohistochemistry. Staining of the WDR37 band in “frost-free” freezers, is not recommended. If slight immunoblotting is specifically inhibited by the turbidity occurs upon prolonged storage, clarify the immunizing protein solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 WD-repeats are motifs that are found in a variety of hours. proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary Product Profile propeller structure. Proteins containing WD-repeats Immunoblotting: a working concentration of participate in a wide range of cellular functions, such as 3.0-6.0 g/mL is recommended using U87 total cell chromatin assembly, cell cycle control, signal extracts. transduction, RNA processing, apoptosis and vesicular trafficking.1 A member of this family, WDR37 (WD Immunofluorescence: a working concentration of repeat- containing protein 37), is a 494 amino acid 2.5-5.0 g/mL is recommended using A431 cells. protein that contains seven WD-repeats. The gene encoding WDR37 maps to human chromosome Note: In order to obtain the best results using various 10q15.3. Interestingly, this gene is located in loci techniques and preparations, we recommend responsible for renal function and chronic kidney determining optimal working dilutions by titration. disease (CKD). This expands the understanding of biologic mechanisms of kidney function by identifying References loci potentially influencing nephrogenesis, podocyte 1. Smith, T.F., Subcell. Biochem., 48, 20-30 (2008). function, angiogenesis, solute transport, and metabolic 2. Köttgen, A., et al., Nat. Genet., 42, 376-384 (2010). functions of the kidney.2 RC,GG,RC,PHC 03/21-1 ©2021 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip. .
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