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i JEOBP JOURNAL OF ESSENTIAL OIL- BEARING PLANTS (ISSN Print: 0972-060X) (ISSN Online: 0976-5026) VOLUME 14 NUMBER 2 March/April 2011 Founder Late Mr. H.K.L. Bhalla Publisher Har Krishan Bhalla & Sons 7/1/2C, Prem Nagar, P.O. Prem Nagar Dehra Dun- 248007 (Uttaranchal) INDIA Web site: www.jeobp.com E-mail: <[email protected]> <[email protected]> Cited in: Analytical Abstracts, Cambridge Scientific Abstracts, Chemical Abstracts, Elsevier SCOPUS, EMBiology, Science Citation Index Expanded (Thomson ISI), Forestry Abstracts, Medicinal and Aromatic Plant Abstracts ii JEOBP JOURNAL OF ESSENTIAL OIL- BEARING PLANTS EDITORS AND EDITORIAL BOARD Hony. Chief Editor Prof. C.S. Mathela Emeritus Professor Chemistry Department of Chemistry Kumaun University, Nainital, India-263002 (India) Managing Editor Arvinder Singh Bhalla 7/1/2C, Prem Nagar, P.O. Prem Nagar, Dehradun- 248007 (India) e-mail: <[email protected]> <[email protected]> EDITORIAL BOARD Prof. E.A. Aboutable Prof. Mitsuo Miyazawa Cairo University (Egypt) Kinki University, Osaka (Japan) Prof. Chlodwig M. FRANZ Prof. J.C. Chalchat University of Veterinary Medicine Vienna University Blaise Pascal de clermont Vienna (Austria) 63177 Aubiere Cedex (France) Prof. C. Menut Prof. Jose Guilherme Maia Universite Montpellier II, (France) DEQAL - UFPA, Belem, PA, (Brazil) Prof. Gerhard Buchbauer Prof. Naoharu Watanabe University of Vienna, (Austria) Shizuoka University (Japan) Prof. Victor David Dr. Leopold Jirovetz Univ. of Bucharest (Romania) University of Vienna (Austria) Prof. Pat Sandra Dr. Jorge A. Pino Research Institute of Chromatography Instituto de Investigaciones para la Kennedypark 20, 8500 Kortrijk (Belgium) Industria Alimentaria, La Habana (Cuba) Prof. William N. Setzer Prof. Fatemeh Sefidkon University of Alabama in Huntsville Res. Inst. of Forests and Rangelands Huntsville, AL 35899 (USA) Tehran (Iran) Dr. Gopal Rao Mallavarapu Prof. Jeno Bernath A-602, Renaissance Temple Bells, Corvinus University of Budapest Yeshwanthapur, Bangalore (India) Budapest (Hungary) iii Prof. Farid CHEMAT Dr. Chandi C. Rath Université d’Avignon North Orrisa University Avignon (France) Takatpur, Baripada, Orrisa (India) Prof. Zrira Saadia Prof. Giuseppe Alonzo Institut Agronomique et Veterinaire Univ. delgi studi di Palermo Hassan II, Rabat (Morocco) Palermo (Italy) Prof. N.Y. Osee Muyima Prof. M. Ali University of Fort Hare Jamia Hamdard (Hamdard University) Alice (South Africa) New Delhi (India) Prof. Mehmet Musa ÖZCAN Dr. Danuta Kalemba Selcuk University Inst. of General Food Chemistry Konya (Turkey) Technical Univ. Lodg (Poland) Dr. Igor Jerkovic Prof. Dr. Azza Amin Ezz El - Din University of Split, 21000 Split National Research Center (Croatia) Dokki, Cairo, Egypt Prof. Gurdip Singh Prof. Bruno Marongiu DDU, Gorakhpur University Università degli Studi di Cagliari Gorakhpur (India) Monserrato (Cagliari), Italy Dr. Nebojsa Simic Dr. V.K. Varshney Norwegian University of Science Forest Research Institute and Technology, Trondheim (Norway) Dehra Dun (India) Dr. Inder Pal Singh Dr. Lee Seong Wei National Institute of Pharmaceutical Education Universiti Malaysia Terengganu and Research (NIPER), Mohali (India) Terengganu (Malaysia) Dr. Hema Lohani Dr. B.R. Rajeswara Rao Centre for Aromatic Plants Central Institute of Medicinal and Dehradun (India) Aromatic Plants, Hyderabad (India) iv JEOBP JOURNAL OF ESSENTIAL OIL- BEARING PLANTS Volume 14 Number 2 March - April 2011 CONTENTS 1. Thujone-Rich Essential Oils of Artemisia rutifolia Stephan ex Spreng. Growing Wild in 136 Tajikistan by Farukh S. Sharopov and William N. Setzer (Tajikistan, USA). 2. Acetylcholinesterase Inhibition Activity of Portuguese Thymus Species Essential Oils by 140 Susana A. Dandlen, Maria G. Miguel, João Duarte, Maria L. Faleiro, Maria J. Sousa, Ana S. Lima, Ana C. Figueiredo, José G. Barroso, Luís G. Pedro (Portugal). 3. Influence of Summer Savory Essential Oil (Satureja hortensis) on Decay of Strawberry 151 and Grape by Neslihan Dikbas, Fatih Dadasoglu, Recep Kotan, Ahmet Cakir (Turkey). 4. Volatile Constituents from Leaves of Justicia pectoralis Jacq. var. tipo by Jorge A. Pino 161 (Cuba). 5. Effect of Ionizing Irradiation on Origanum Leaves (Origanum vulgare L.) Essential Oil 164 Composition by Juan J. Elizalde , Mónica Espinoza (Chile). 6. GC-MS Analysis of Ammoides atlantica (Coss. et Dur.) Wolf. from Algeria by Tarek - 172 Boudiar, Chawki Bensouici, Javad Safaei-Ghomi, Ahmed Kabouche and Zahia - Kabouche (Algeria, Iran). 7. Assessment of the Preservative Activity of Some Essential Oils to Reduce Postharvest 175 Fungal Rot on Kiwifruits (Actinidia deliciosa) by Habib Shirzad, Abbas Hassani, Ali Abdollahi, Youbert Ghosta, Seied Rasool Finidokht (Iran). 8. Chemical Composition and Antimicrobial Study of Essential Oil from the Leaves of 185 Curcuma haritha Mangaly and Sabu by Gopan Raj, Nediyaparambu S. Pradeep, Mathew Dan, Mathur G. Sethuraman and Varughese George (India). 9. Composition and Bioactivity of Essential Oils from Leaves and Fruits of Myrtus communis 192 and Eugenia supraxillaris (Myrtaceae) Grown in Egypt by E.A. Aboutabl, K.M. Meselhy, E.M. Elkhreisy, M.I.Nassar and R. Fawzi (Egypt). 10. Chemical Composition of the Essential Oil from Aerial Parts of Haplophyllum acutifolium 201 (DC.) G. Don from Iran by Javad Asili, Maryam Rajae Fard, Ali Ahi and Seyyed Ahmad Emami (Iran). 11. Chemical Composition and Antimicrobial Activity of the Essential Oil of Mentha pulegium 208 L. by K. Morteza-Semnani, M. Saeedi and Mohammad Akbarzadeh (Iran). 12. The Effect of Microwaves on Essential oils of White and Black Pepper (Piper nigrum L.) 214 and their Antioxidant Activities by Magda A. Abd El Mageed, Amr F. Mansour, Khaled F. El Massry, Manal M. Ramadan, Mohamed S. Shaheen (Egypt). v 13. Volatile Constituents of Chromolaena odorata (L.) R.M. King & H. Rob. Leaves from 224 Benin by Cosme Kossouoh, Mansour Moudachirou, Victor Adjakidje, Jean-Claude Chalchat, Gilles Figuérédo, Pierre Chalard (Benin, France). 14. Bioconversion of Essential Oil from Plants with Eugenol Bases to Vanillin by Serratia- 229 marcescens by A. Khanafari, M. Seyed Jafari Olia F. Sharifnia (Iran). 15. Essential Oil Composition and Antibacterial Activity of Nepeta glomerulosa Boiss from 241 Iran by Azizollah Nezhadali, Mahboobeh Masrornia, Hossein Bari, Mina Akbarpour, Mohammad Reza Joharchi and Mahboobeh Nakhaei -Moghadam (Iran). 16. Chemical Composition of Essential Oil from Doum Fruits Hyphaene thebaica (Palmae) by 245 Nahla A. Ayoub, Omayma A. Eldahshan, Abdel-Nasser B. Singab and Mohamed M. Al- Azizi (Egypt). 17. Comparison of Essential Oils from Ferula ovina (Boiss.) Aerial Parts in Fresh and Dry 250 Stages by Hossein Azarnivand, Marzieh Alikhah-Asl, Mohammad Jafari, Hossein Arzani, Gholamreza Amin, Seyed Saeed Mousavi (Iran). 18. In vitro Antibacterial and Antifungal Activity of Salvia multicaulis by Taran Mojtaba, 255 Ghasempour Hamid Reza, Safoora Borzo, Najafi Shiva, Samadian Esmaeil (Iran). Jeobp 14 (2) 2011 pp 136 - 139 136 Journal of Essential Oil Bearing Plants ISSN Print: 0972-060X Online: 0976-5026 www.jeobp.com Thujone-Rich Essential Oils of Artemisia rutifolia Stephan ex Spreng. Growing Wild in Tajikistan Farukh S. Sharopov 1 and William N. Setzer 2* 1 V. I. Nikinin Institute of Chemistry, Tajik Academy of Sciences, Ainy St. 299/2, Dushanbe, 734063, Tajikistan 2 Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA Received 27 September 2010; accepted in revised form 18 January 2011 Abstract: The essential oil from the aerial parts of Artemisia rutifolia Stephan ex Spreng., collected from two different regions of Tajikistan, were obtained by hydrodistillation and analyzed by GC-MS. A total of 77 compounds were identified in the oils, accounting for 98.6 % and 99.2 % of the two oils. Both essential oils were dominated by α-thujone (20.9 % and 36.6 %) and β-thujone (47.3 % and 36.1 %) with lesser amounts of 1,8-cineole (3.2 % and 11.7 %) and germacrene D (2.8 % and 1.8 %). Key words: Artemisia rutifolia, essential oil composition, thujone, Tajikistan. Introduction: Artemisia rutifolia Stephan ex Spreng. is a member of the Asteraceae (Compositae) and is distributed in Afghanistan, China, India, Kazakhstan, Kyrgyzstan, Mongolia, Nepal, Pakistan, Russian Federation and Tajikistan 1. The plant is an important traditional medicine. An infusion of the herb is taken to relieve painful urination; the fresh herb is used as an analgesic for toothache; the dried herb is used to treat excessive sweating; a decoction of the herb is gargled for treatment of angina, stomach problems, and heart problems. A. rutifolia essential oil has antibacterial, antifungal and anthelmintic activity 2. The essential oil of A. rutifolia growing in the Pamirs had been previously investigated by Goryaev in 1962, who reported the main constituents to be 1,8-cineole, α- and β-thujone, camphor, α- and β-pinene, camphene, and limonene 3. Shavarda examined A. rutifolia oil from the Mongolian People’s Republic and identified 15 components: 1,8-cineole (35.0 %), camphor (18.0 %), α- and β- thujone (11.0 %), terpinen-4-ol (7.0 %), α-terpineol (5.0 %), α-pinene, β-pinene, camphene, limonene, β-phellandrene, p-cymene, 4-phenylbutan-2-one, 4-phenyl-butan-2-ol, and 4-phenylbut-2-yl acetate 4. Experimental Plant material: Aerial parts of Artemisia rutifolia were collected from two regions of Tajikistan: Sample #1, the Khonaobod village, Muminobod region (38.107547 N, 69.966431 E, 1200 m above sea level), on 2 May 2010; Sample #2, the Chormaghzak