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PSMB5 Antibody Cat PSMB5 Antibody Cat. No.: 57-791 PSMB5 Antibody Western blot analysis of PSMB5 using rabbit polyclonal PSMB5 Antibody immunohistochemistry analysis in PSMB5 Antibody using 293 cell lysates (2 ug/lane) either formalin fixed and paraffin embedded human skin tissue nontransfected (Lane 1) or transiently transfected (Lane 2) followed by peroxidase conjugation of the secondary with the PSMB5 gene. antibody and DAB staining. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human This PSMB5 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 235-263 amino acids from the C-terminal region of human PSMB5. TESTED APPLICATIONS: IHC-P, WB For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50 October 1, 2021 1 https://www.prosci-inc.com/psmb5-antibody-57-791.html PREDICTED MOLECULAR 28 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: PSMB5 Proteasome subunit beta type-5, Macropain epsilon chain, Multicatalytic endopeptidase ALTERNATE NAMES: complex epsilon chain, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit MB1, Proteasome subunit X, PSMB5, LMPX, MB1, X ACCESSION NO.: P28074 PROTEIN GI NO.: 187608890 GENE ID: 5693 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An BACKGROUND: essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. October 1, 2021 2 https://www.prosci-inc.com/psmb5-antibody-57-791.html REFERENCES: 1) Ri, M., et al. Leukemia 24(8):1506-1512(2010) 2) Lu, S., et al. Exp. Hematol. 37(7):831-837(2009) 3) Lu, S., et al. Exp. Hematol. 36(10):1278-1284(2008) 4) Oerlemans, R., et al. Blood 112(6):2489-2499(2008) ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 1, 2021 3 https://www.prosci-inc.com/psmb5-antibody-57-791.html.
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