PSMB5 Antibody Cat. No.: 57-791

PSMB5 Antibody

Western blot analysis of PSMB5 using rabbit polyclonal PSMB5 Antibody immunohistochemistry analysis in PSMB5 Antibody using 293 cell lysates (2 ug/lane) either formalin fixed and paraffin embedded human skin tissue nontransfected (Lane 1) or transiently transfected (Lane 2) followed by peroxidase conjugation of the secondary with the PSMB5 . antibody and DAB staining.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

This PSMB5 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 235-263 amino acids from the C-terminal region of human PSMB5.

TESTED APPLICATIONS: IHC-P, WB

For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50

October 1, 2021 1 https://www.prosci-inc.com/psmb5-antibody-57-791.html PREDICTED MOLECULAR 28 kDa WEIGHT:

Properties

This antibody is purified through a A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: PSMB5

Proteasome subunit beta type-5, Macropain epsilon chain, Multicatalytic endopeptidase ALTERNATE NAMES: complex epsilon chain, chain 6, Proteasome epsilon chain, Proteasome subunit MB1, Proteasome subunit X, PSMB5, LMPX, MB1, X

ACCESSION NO.: P28074

PROTEIN GI NO.: 187608890

GENE ID: 5693

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/-dependent process in a non-lysosomal pathway. An BACKGROUND: essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.

October 1, 2021 2 https://www.prosci-inc.com/psmb5-antibody-57-791.html REFERENCES: 1) Ri, M., et al. Leukemia 24(8):1506-1512(2010)

2) Lu, S., et al. Exp. Hematol. 37(7):831-837(2009)

3) Lu, S., et al. Exp. Hematol. 36(10):1278-1284(2008)

4) Oerlemans, R., et al. Blood 112(6):2489-2499(2008)

ANTIBODIES FOR RESEARCH USE ONLY.

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October 1, 2021 3 https://www.prosci-inc.com/psmb5-antibody-57-791.html