Identification of Antikinetoplastid Compounds From
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IDENTIFICATION OF ANTIKINETOPLASTID COMPOUNDS FROM PSOROTHAMNUS POLYDENIUS AND P. ARBORESCENS DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University BY Manar Mahfouz Salem Hemida, B.S., M.S. * * * * * THE OHIO STATE UNIVERSITY 2005 Dissertation Committee: Approved By Professor Karl A. Werbovetz, Adviser Professor A. Douglas Kinghorn Professor Ching-Shih Chen Adviser Graduate Program in Pharmacy Copyright by Manar Mahfouz Salem Hemida 2005 ABSTRACT Parasitic diseases such as leishmaniasis and trypanosomiasis remain a major public health problem. They exact a heavy toll of illness and death especially in developing countries. Common chemotherapeutic agents currently used are often inadequate, requiring long courses of parenteral administration, having toxic side effects or are becoming less effective due to the emergence of resistant strains. Thus, there is an urgent need for new, effective, and inexpensive drugs. Natural products are important sources of novel therapeutic agents in the fight against parasitic diseases. The goal of our research is to identify effective antileishmanial and trypanocidal agents from plant sources using a systematic approach. A natural products library of a representative diversity was screened for antiparasitic activity against Leishmania donovani and Trypanosoma brucei brucei. The active samples were further evaluated regarding their toxicity versus mammalian cell lines. From the results, the plant genus Psorothamnus was identified as a promising source of potential new antiparasitic compounds. ii Bioactivity-guided fractionation of the methanolic extract of Psorothamnus polydenius yielded the new chalcone, 2,2′,4′-trihydroxy-6′-methoxy-3′,5′- dimethylchalcone, together with six other known compounds, 2′,4′-dihydroxy-6′- methoxy-3′,5′-dimethylchalcone, dalrubone, demethoxymatteucinol, eriodictyol, oleanolic acid and photodalrubone. This was the first report of chalcones in P. polydenius. The extracts and isolated compounds were tested in vitro for their antiprotozoal activity against Leishmania donovani and Trypanosoma brucei. The isolated chalcones exhibited leishmanicidal (IC50 5.0–7.5 µg/mL, respectively) and trypanocidal (IC50 6.3–6.8 µg/ml, respectively) properties. Dalrubone displayed 6-fold selectivity for axenic L. donovani parasites over vero cells. Furthermore, treatment of L. mexicana-preinfected macrophages with the chalcones (12.5 µg/mL) or dalrubone (25 µg/mL) reduced the number of infected macrophages by at least 96% while posing no toxicity to the host cell. Flow cytometry analysis showed that dalrubone does not affect the cell cycle progression of the parasite. Electron microscopy revealed significant ultrastructural changes in the parasite suggesting perturbations in the secretory pathways of the parasite. However, dalrubone did not display a favorable in vivo activity against L. donovani infection in mice, possibly because of a low potency. Another member of the same genus, P. arborescens, exhibited significant activity against Leishmania donovani axenic amastigotes and Trypanosoma brucei brucei bloodstream forms. Bioactivity-guided fractionation of the root extract of P. arborescens yielded the new isoflavone 5,7,3΄,4΄-tetrahydroxy-2΄-(3,3-dimethylallyl)isoflavone (5.1a) and the new 2-arylbenzofuran 2-(2΄-hydroxy-4΄,5΄-methylenedioxyphenyl)-6- methoxybenzofuran-3-carbaldehyde (5.7), together with seven other known compounds, iii fremontin, glycyrrhisoflavone, calycosin, maackiain, 4-hydroxymaackiain, oleanolic acid, and isoliquiritigenin. In addition, the structure of the isoflavone fremontin was revised using spectroscopic and chemical methods and was assigned a new structure. The isoflavone 5.1a and isoliquiritigenin displayed IC50 values of 4.6 and 5.3 μg/mL, respectively, against L. donovani axenic amastigotes. Calycosin exhibited selective toxicity against T. b. brucei (IC50 3.6 μg/mL) compared to L. donovani amastigotes and Vero cells (IC50 values 28.5 and 45.1 μg/mL, respectively). These results prompted us to test a small group of structurally-related isoflavones for their antitrypanosomal activities. Genistein and 7,3΄,4΄-trihydroxyisoflavone displayed promising activity (IC50 values 1.1 and 1.9 μg/mL, equivalent to 4.2 and 7.1 μM, respectively) and selectivity (IC50 values 33 and 135 μM, respectively, versus Vero cells). The mechanism of the antikinetoplastid activity of isoflavonoids is not clear although electron microscopy studies indicated that treatment of L. donovani promastigotes with isoflavone 5.1a resulted in significant changes in the mitochondrial ultrastructure. Our results suggest that the isoflavone skeleton deserves further investigation as a template for novel antileishmanial and trypanocidal compounds. iv Dedicated to my parents v ACKNOWLEDGMENTS I sincerely thank my adviser, Dr. Karl A. Werbovetz, for his continuous support, guidance, and encouragement. I am especially grateful that he gave me the opportunity to pursue my research ideas and for always being there for advice and assisstance. I wish to thank Dr. John M. Cassady for granting access to his collection of natural products and for helping me obtain the plant samples used in this work. I am grateful to Dr. Richard W. Spjut for the collection and identification of the botanical material used in my dissertation and for the invaluable comments and suggestions regarding the taxonomy and botany of the samples he collected. I also thank Dr. Nanjun Sun in Dr. Cassady’s laboratory for the preparation of the extracts that were used in the initial screening process in this work. I would like to express my gratitude to Dr. Raymond W. Doskotch for his help, advice and inspiration, Dr. Robert W. Curley for discussions and help with the HPLC equipment, Mr. Jack Fowble for training on the NMR instrumentation. I would like also to thank Dr. Charles E. Cottrell for the valuable discussions and help on NMR techniques. I also thank Dr. Lucia E. Rosas for help and training to obtain murine macrophages. I would like to thank Kathy Brooks for all her help throughout my graduate years. vi I am deeply thankful to Dr. A. Douglas Kinghorn and Dr. Ching-Shih Chin for serving as members of my dissertation committee. I wish also to thank the faculty in the Division of Medicinal Chemistry and Pharmacognosy for excellent and enthusiastic teaching. I wish to express my gratitude to my friends and colleagues in the College of Pharmacy, especially former and present members of the Werbovetz laboratory, Dr. Denise Cottrell, Dr. Gautam Bhattacharya, Dr. Tesmol George, Adam Yakovitch and Dawn Delfin. I am also grateful for the support I received from my long-time friends and colleagues. My deepest gratitude and appreciation go to my parents for their love, inspiration, and always believing in me. Words can not adequately express my gratitude towards my husband, Mohammad, who endured many sacrifices during my graduate years. Without his love and endless support I would not have been able to accomplish this dissertation. I am grateful to my children, Basel and Nadine, for filling my life with joy and motivation. Furthermore, I wish to thank my brothers, sister, and the rest of my family for the support and encouragement. Most of all, I praise God for blessing me with the people in my life and for giving me the opportunity and ability to complete this dissertation. vii VITA October 29, 1972………….. Born – Shebin El-Koum, Egypt 1995 ………………………. B.S. Pharmacy, Tanta University, Egypt 1996 – 1997……………….. Instructor, College of Pharmacy, Tanta University, Egypt 1998 – 2000 ……………… Intern then Registered Pharmacist, Columbus, Ohio 2000 – 2002……………….. Graduate Teaching Associate, Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University 2003……………………….. M.S. Pharmacy, The Ohio State University 2002 – 2005……………….. Graduate Fellow, Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University PUBLICATIONS 1. Salem, Manar M. and Werbovetz, Karl A. Antiprotozoal compounds from Psorothamnus polydenius. Journal of Natural Products 2005, 68, 108-111. viii 2. Cottrell, Denise M.; Capers, Jeffrey; Salem, Manar M.; DeLuca-Fradley, Kate; Croft, Simon L.; Werbovetz, Karl A. Antikinetoplastid activity of 3-aryl-5- thiocyanatomethyl-1,2,4-oxadiazoles. Bioorganic & Medicinal Chemistry 2004, 12, 2815-2824. 3. Bhattacharya, Gautam; Herman, Johnathan; Delfin, Dawn; Salem, Manar M.; Barszcz, Todd; Mollet, Mike; Riccio, Guy; Brun, Reto; Werbovetz, Karl A. Synthesis and antitubulin activity of N1- and N4-substituted 3,5-dinitro sulfanilamides against African trypanosomes and Leishmania. Journal of Medicinal Chemistry 2004, 47, 1823-1832. 4. Werbovetz, Karl A.; Sackett, Dan L.; Delfin, Dawn; Bhattacharya, Gautam; Salem, Manar; Obrzut, Tomasz; Rattendi, Donna; Bacchi, Cyrus. Selective antimicrotubule activity of N1-phenyl-3,5-dinitro-N4,N4-di-n-propylsulfanilamide (GB-II-5) against kinetoplastid parasites. Molecular Pharmacology 2003, 64, 1325-1333. 5. Hua, Duy H.; Tamura, Masafumi; Egi, Masahiro; Werbovetz, Karl; Delfin, Dawn; Salem, Manar; Chiang, Peter K. Antiprotozoal activities of symmetrical bishydroxamic acids. Bioorganic & Medicinal Chemistry 2003, 11, 4357-4361. 6. Stephens, Chad E.; Brun, Reto; Salem, Manar M.; Werbovetz, Karl A.; Tanious, Farial; Wilson, W. David; Boykin, David W. The activity of diguanidino and “reversed” diamidino 2,5-diarylfurans versus Trypanosoma cruzi and Leishmania donovani. Bioorganic &