Key Factors Influencing the Occurrence and Frequency of Ciguatera
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Development of a Quantitative PCR Assay for the Detection And
bioRxiv preprint doi: https://doi.org/10.1101/544247; this version posted February 8, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Development of a quantitative PCR assay for the detection and enumeration of a potentially ciguatoxin-producing dinoflagellate, Gambierdiscus lapillus (Gonyaulacales, Dinophyceae). Key words:Ciguatera fish poisoning, Gambierdiscus lapillus, Quantitative PCR assay, Great Barrier Reef Kretzschmar, A.L.1,2, Verma, A.1, Kohli, G.S.1,3, Murray, S.A.1 1Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia 2ithree institute (i3), University of Technology Sydney, Ultimo, 2007 NSW, Australia, [email protected] 3Alfred Wegener-Institut Helmholtz-Zentrum fr Polar- und Meeresforschung, Am Handelshafen 12, 27570, Bremerhaven, Germany Abstract Ciguatera fish poisoning is an illness contracted through the ingestion of seafood containing ciguatoxins. It is prevalent in tropical regions worldwide, including in Australia. Ciguatoxins are produced by some species of Gambierdiscus. Therefore, screening of Gambierdiscus species identification through quantitative PCR (qPCR), along with the determination of species toxicity, can be useful in monitoring potential ciguatera risk in these regions. In Australia, the identity, distribution and abundance of ciguatoxin producing Gambierdiscus spp. is largely unknown. In this study we developed a rapid qPCR assay to quantify the presence and abundance of Gambierdiscus lapillus, a likely ciguatoxic species. We assessed the specificity and efficiency of the qPCR assay. The assay was tested on 25 environmental samples from the Heron Island reef in the southern Great Barrier Reef, a ciguatera endemic region, in triplicate to determine the presence and patchiness of these species across samples from Chnoospora sp., Padina sp. -
Circulation and Suspended Sediment Transport in a Coral
Marine Pollution Bulletin Archimer 2010, Volume 61, Issues 7-12, Pages 269-296 http://archimer.ifremer.fr http://dx.doi.org/10.1016/j.marpolbul.2010.06.023 © 2010 Elsevier Ltd All rights reserved. ailable on the publisher Web site Circulation and suspended sediment transport in a coral reef lagoon: The south-west lagoon of New Caledonia S. Ouillona,b,*, P. Douilletc, J.P. Lefebvrec, R. Le Gendred, A. Jouone, P. Bonnetone, J.M. Fernandezd, C. Chevillond, O. Magandf, J. Lefèvred, P. Le Hirg, R. Laganierh, F. Dumasg, P. Marchesielloa,d, A. Bel Madanii, S. Andréfouëtd, J.Y. Panchéd, R. Fichezc a Université de Toulouse, UPS (OMP-PCA), LEGOS, 14 av. Edouard Belin, Toulouse F-31400, France b IRD, LEGOS, 14 av. Edouard Belin, Toulouse F-31400, France c IRD, UAM, Dpt Hidrobio – DIV de Ciencas Bio y Salud, AV. San Rafael Atlixco 186 – Col. Vicentina 09340, Mexico DF, Mexico blisher-authenticated version is av d IRD Nouméa, BP A5, 98848 Nouméa cedex, New Caledonia e Université de Bordeaux, CNRS, UMR 5805, Bordeaux F-33000, France f LGGE, CNRS, Université Joseph Fourier, 54 rue Molière, Saint Martin d’Hères cedex F-38402, France g DYNECO/PHYSED, Ifremer, BP 70, Plouzané F-29280, France h Université Paris Diderot – Paris 7, Case 7001, 75205 Paris cedex 13, France i IRD, IMARPE/CIMOBP, Instituto del Mar del Perù, Esquina de Gamarra y General Valle S/N Chucuito, Callao, Peru *: Corresponding author : S. Ouillon, Tel.: +33 5 61 33 29 02, email address : [email protected] Abstract: The south-west lagoon of New Caledonia is a wide semi-open coral reef lagoon bounded by an intertidal barrier reef and bisected by numerous deep inlets. -
New Record of the Chinamanfish, Symphorus
KOREAN JOURNAL OF ICHTHYOLOGY, Vol. 27, No. 2, 149-152, June 2015 Received: April 17, 2015 ISSN: 1225-8598 (Print), 2288-3371 (Online) Revised: June 8, 2015 Accepted: June 24, 2015 New Record of the Chinamanfish,Symphorus nematophorus (Perciformes: Lutjanidae) from Korea By Maeng Jin Kim, Tae Seok Mun1, Jeong-Hoon Lee1, Young Min Choi and Choon Bok Song2,* West Sea Fisheries Research Institute, National Fisheries Research and Development Institute (NFRDI), Incheon 400-420, Korea 1Southeast Sea Fisheries Research Institute, NFRDI, Tongyeong 690-943, Korea 2College of Ocean Sciences, Jeju National University, Jeju City 690-756, Korea ABSTRACT A single specimen of Symphorus nematophorus (481.0 mm in SL), belonging to the family Lutjanidae, was firstly collected by a set net in the coastal waters of Geojae Island, located in south sea of Korea on August 1, 2014. This specimen was characterized by having the anterior dorsal soft rays produced into filaments, a pit between eye and nostril, no tooth on vomer, and nine longitudinal blue strips on both sides of the body. We propose a new Korean name, “Sil-tung-dom-sok” and “Sil-tung-dom” for the genus and species, respectively. Key words: Lutjanidae, Symphorus nematophorus, new record, Geojae Island, Korea INTRODUCTION MATERIALS AND METHODS The snapper fish (family Lutjanidae), belonging to the The specimen was preserved in 10% formalin for a order Perciformes, comprises 185 species in 17 genera week and then transferred to 70% ethanol. Counts and worldwide (Nelson, 2006). Among them, 13 species in measurements are followed by the methods of Hubbs and five genera were known in Korea (Kim et al., 2005, 2012). -
Appendices Appendices
APPENDICES APPENDICES APPENDIX 1 – PUBLICATIONS SCIENTIFIC PAPERS Aidoo EN, Ute Mueller U, Hyndes GA, and Ryan Braccini M. 2015. Is a global quantitative KL. 2016. The effects of measurement uncertainty assessment of shark populations warranted? on spatial characterisation of recreational fishing Fisheries, 40: 492–501. catch rates. Fisheries Research 181: 1–13. Braccini M. 2016. Experts have different Andrews KR, Williams AJ, Fernandez-Silva I, perceptions of the management and conservation Newman SJ, Copus JM, Wakefield CB, Randall JE, status of sharks. Annals of Marine Biology and and Bowen BW. 2016. Phylogeny of deepwater Research 3: 1012. snappers (Genus Etelis) reveals a cryptic species pair in the Indo-Pacific and Pleistocene invasion of Braccini M, Aires-da-Silva A, and Taylor I. 2016. the Atlantic. Molecular Phylogenetics and Incorporating movement in the modelling of shark Evolution 100: 361-371. and ray population dynamics: approaches and management implications. Reviews in Fish Biology Bellchambers LM, Gaughan D, Wise B, Jackson G, and Fisheries 26: 13–24. and Fletcher WJ. 2016. Adopting Marine Stewardship Council certification of Western Caputi N, de Lestang S, Reid C, Hesp A, and How J. Australian fisheries at a jurisdictional level: the 2015. Maximum economic yield of the western benefits and challenges. Fisheries Research 183: rock lobster fishery of Western Australia after 609-616. moving from effort to quota control. Marine Policy, 51: 452-464. Bellchambers LM, Fisher EA, Harry AV, and Travaille KL. 2016. Identifying potential risks for Charles A, Westlund L, Bartley DM, Fletcher WJ, Marine Stewardship Council assessment and Garcia S, Govan H, and Sanders J. -
Of Spermonde Archipelago, South Sulawesi
Jurnal Iktiologi Indonesia, 10(1): 83-92, 2010 THE SNAPPER (LUTJANIDAE) OF SPERMONDE ARCHIPELAGO, SOUTH SULAWESI Andi Iqbal Burhanuddin1 and Yukio Iwatsuki2 1 Laboratory of Marine Biology, Faculty of Marine Science and Fisheries, Hasanuddin University 2 Division of Fisheries Science, Faculty of Agriculture, Miyazaki University, Japan Faculty of Marine Science and Fisheries, Hasanuddin University Jl. Perintis Kemerdekaan Km 10 Makassar 90245 correspondence author e-mail: [email protected] Received: 12 Mei 2010, Accepted: 15 Juni 2010 ABSTRACT Fish diversity monitoring could be used as a basis for formulating management of the fisheries resources. This study was conducted to describe the snapper of the family Lutjanidae from the Spermonde Archipelago, South Sulawesi. Spe- cimens were collected in Rajawali and Paotere Fish Landing Port Makassar, South Sulawesi from November 2005 to August 2009. The result showed that there was 42 species representing eight genera of family Lutjanidae inhabiting the area were examined and identified: Aphareus furca, A. rutilans, Aprion virescens, Etelis carbunculus, E. radiosus, Lut- janus argentimaculatus, L. bengalensis, L. biguttatus, L. bohar, L. boutton, L. carponotatus, L. decussatus, L. fulvus, L. sebae, L. fulviflamma, L. fuscescens. L. johnii, L. kasmira, L. gibbus, L. lemniscatus, L. lunulatus, L. lutjanus, L. mala- baricus, L. monostigma, L. quinquelineatus, L. rivulatus, L. russelli, L. sebae, L. timorensis, L. vitta, Macolor macularis, M. niger, Paracaesio kusakarii, Pristipomoides argyrogrammicus, P. auricilla, P. filamentosus, P. flavipinnis, P. mul- tidens, P. typus, P. zonatus, Symphorichthys spilurus, S. nematophorus. The local name available of each species was given. Key words: Lutjanidae, Snapper, South Sulawesi, Spermonde. INTRODUCTION diversity monitoring and could hopefully be used Spermonde archipelago (Spermonde shelf) as a basic data for formulating management regu- located in the Southern Makassar Strait, south- lation of the fisheries resources for the Spermon- west coast of Sulawesi Island. -
Proceedings of the 2008 Marine and Tropical Sciences Research Facility Annual Conference
Proceedings of the 2008 Marine and Tropical Sciences Research Facility Annual Conference 28 April – 1 May 2008 The Hotel Cairns, Abbott Street, Cairns Compiled by Robin Taylor1 and Suzanne Long2 1 Consultant with Page One 2 Reef and Rainforest Research Centre Limited Supported by the Australian Government’s Marine and Tropical Sciences Research Facility © Reef and Rainforest Research Centre Limited ISBN 9781921359279 This report should be cited as: Taylor, R. and Long, S. (2009) Proceedings of the 2008 Marine and Tropical Sciences Research Facility Annual Conference, 28 April – 1 May 2008. Reef and Rainforest Research Centre Limited, Cairns (230pp.). Sections of this report should be cited as, for example: Carmody, J. and Prideaux, B. (2009) Visitation and recreation in the Wet Tropics World Heritage Area: A comparison of urban and regional residents. In: Proceedings of the 2008 Marine and Tropical Sciences Research Facility Annual Conference, 28 April – 1 May 2008. Taylor, R. and Long, S. (eds.) Reef and Rainforest Research Centre Ltd, Cairns. Published by the Reef and Rainforest Research Centre on behalf of the Australian Government’s Marine and Tropical Sciences Research Facility. The Australian Government’s Marine and Tropical Sciences Research Facility (MTSRF) supports world-class, public good research. The MTSRF is a major initiative of the Australian Government, designed to ensure that Australia’s environmental challenges are addressed in an innovative, collaborative and sustainable way. The MTSRF investment is managed by the Department of the Environment, Water, Heritage and the Arts (DEWHA), and is supplemented by substantial cash and in-kind investments from research providers and interested third parties. -
Talkabout October / November 2
100th Edition !!!!! October/November 2011 INNISFAIL & DISTRICT Community Information Newsletter Produced by the WEB PAGE COMMUNITY SUPPORT CENTRE www.csci.org.au 13-17 Donald Street, P O Box 886, Innisfail 4860 Phone: 40438400 Fax: 4061 7312 Freecall: 1800 616 001 Email: [email protected] The Community Support Centre is funded primarily by the Department of Communities We look back at the best... DISCLAIMER ALL ARTICLES IN THIS MAGAZINE ARE PRINTED IN GOOD FAITH FOR THE COMMUNITY AND DO NOT NECESSARILY REPRESENT THE VIEWS OF THE INNISFAIL COMMUNITY SUPPORT CENTRE INC. THE CENTRE ACCEPTS NO RESPONSIBILITY FOR THESE ARTICLES. CONTENTS Page 2 Editorial etc Page 7 Rocky Creek Page 13-17 Local Stories Page 3 CAIC & Community Development Page 8 Gwen’s Story Page 18 Recipes Page 4 Show Society & Historical Society Page 9 Volunteers Page 5 The Mount Mulligan Disastor Page 10-11 Local Stories Page 6 Early Days of Flying Fish Point Page 12 Loss of an Icon & Fireman’s Story Talkabout October / November 2 Mary Camilleri who joined Gwen more than 10 years ago, and with her skills in using the MS Editorial Publisher program, sets the layout of Talkabout. Wow! Here we are with the 100th issue of our Phil Pearce has also taken on a dedicated little magazine—and how proud I am to be part Talkabout role. He now distributes some 2,000 of the team that helps produce it. It is copies every 2nd month to over 90 local outlets! distributed to a wide area of the Innisfail, In celebrating this 100th edition we decided to Babinda and Mission Beach areas and I’d publish a selection of the personal interest better include Miriwinni as they rang up for articles that have been printed over the years. -
Further Advance of Gambierdiscus Species in the Canary Islands, with the First Report of Gambierdiscus Belizeanus
toxins Article Further Advance of Gambierdiscus Species in the Canary Islands, with the First Report of Gambierdiscus belizeanus Àngels Tudó 1, Greta Gaiani 1, Maria Rey Varela 1 , Takeshi Tsumuraya 2 , Karl B. Andree 1, Margarita Fernández-Tejedor 1 ,Mònica Campàs 1 and Jorge Diogène 1,* 1 Institut de Recerca i Tecnologies Agroalimentàries (IRTA), Ctra. Poble Nou Km 5.5, Sant Carles de la Ràpita, 43540 Tarragona, Spain; [email protected] (À.T.); [email protected] (G.G.); [email protected] (M.R.V.); [email protected] (K.B.A.); [email protected] (M.F.-T.); [email protected] (M.C.) 2 Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Osaka 599-8570, Japan; [email protected] * Correspondence: [email protected] Received: 22 September 2020; Accepted: 27 October 2020; Published: 31 October 2020 Abstract: Ciguatera Poisoning (CP) is a human food-borne poisoning that has been known since ancient times to be found mainly in tropical and subtropical areas, which occurs when fish or very rarely invertebrates contaminated with ciguatoxins (CTXs) are consumed. The genus of marine benthic dinoflagellates Gambierdiscus produces CTX precursors. The presence of Gambierdiscus species in a region is one indicator of CP risk. The Canary Islands (North Eastern Atlantic Ocean) is an area where CP cases have been reported since 2004. In the present study, samplings for Gambierdiscus cells were conducted in this area during 2016 and 2017. Gambierdiscus cells were isolated and identified as G. australes, G. excentricus, G. caribaeus, and G. -
The Age Natural Disaster Posters
The Age Natural Disaster Posters Wild Weather Student Activities Wild Weather 1. Search for an image on the Internet showing damage caused by either cyclone Yasi or cyclone Tracy and insert it in your work. Using this image, complete the Thinking Routine: See—Think— Wonder using the table below. What do you see? What do you think about? What does it make you wonder? 2. World faces growing wild weather threat a. How many people have lost their lives from weather and climate-related events in the last 60 years? b. What is the NatCatService? c. What does the NatCatService show over the past 30 years? d. What is the IDMC? e. Create a line graph to show the number of people forced from their homes because of sudden, natural disasters. f. According to experts why are these disasters getting worse? g. As human impact on the environment grows, what effect will this have on the weather? h. Between 1991 and 2005 which regions of the world were most affected by natural disasters? i. Historically, what has been the worst of Australia’s natural disasters? 3. Go to http://en.wikipedia.org/wiki/File:Global_tropical_cyclone_tracks-edit2.jpg and copy the world map of tropical cyclones into your work. Use the PQE approach to describe the spatial distribution of world tropical cyclones. This is as follows: a. P – describe the general pattern shown on the map. b. Q – use appropriate examples and statistics to quantify the pattern. c. E – identifying any exceptions to the general pattern. 4. Some of the worst Question starts a. -
Spatial and Temporal Patterns of Flood Plumes in the Great Barrier Reef, Australia
Spatial and temporal patterns of flood plumes in the Great Barrier Reef, Australia Thesis submitted by Michelle Jillian Devlin BSc (Bendigo College of Advanced Education (Latrobe University) Msc (James Cook University) February 2005 Thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Tropical Environment Studies and Geography Department, James Cook University. Spatial and temporal patterns of flood plumes in the Great Barrier Reef, Australia 1 STATEMENT OF ACCESS I, the undersigned, author of this work, understand that James Cook University will make this thesis available for use within the University Library and, via the Australian Digital Theses network, for use elsewhere. I understand that, as an unpublished work, a thesis has significant protection under the Copyright Act and; I do not wish to place any further restriction on access to this work. _________________________ ______________ Signature Date Spatial and temporal patterns of flood plumes in the Great Barrier Reef, Australia 2 STATEMENT OF SOURCES DECLARATION I declare that this thesis is my own work and has not been submitted in any form for another degree or diploma at any university or other institution of tertiary education. Information derived from the published or unpublished work of others has been acknowledged in the text and a list of references is given. ____________________________________ ____________________ Signature Date Spatial and temporal patterns of flood plumes in the Great Barrier Reef, Australia 3 Papers arising from this thesis Devlin , M., Brodie, J, Waterhouse, J., Mitchell, A., Audas, D. and Haynes, D. (2003). Exposure of Great Barrier Reef inner-shelf reefs to river-borne contaminants. -
Recovery Plan for the Southern Cassowary Casuarius Casuarius Johnsonii Prepared by Peter Latch for the Cassowary Recovery Team
Recovery plan for the southern cassowary Casuarius casuarius johnsonii Prepared by Peter Latch for the Cassowary Recovery Team Title: Recovery plan for the southern cassowary Casuarius casuarius johnsonii Prepared by: Peter Latch for the Cassowary Recovery Team © The State of Queensland, Environmental Protection Agency, 2007 Copyright protects this publication. Except for purposes permitted by the Copyright Act, reproduction by whatever means is prohibited without the prior written knowledge of the Environmental Protection Agency. Inquiries should be addressed to PO Box 15155, CITY EAST, QLD 4002. Copies may be obtained from the: Executive Director Sustainable Communities Environmental Protection Agency PO Box 15155 CITY EAST QLD 4002 Disclaimer: The Australian Government, in partnership with the Environmental Protection Agency, facilitates the publication of recovery plans to detail the actions needed for the conservation of threatened native wildlife. This State approved recovery plan was prepared with financial support form the Australian Government and has been adopted as a National Recovery Plan under the provisions of the Commonwealth Environment Protection and Biodiversity Conservation Act 1999. The attainment of objectives and the provision of funds may be subject to budgetary and other constraints affecting the parties involved, and may also be constrained by the need to address other conservation priorities. Approved recovery actions may be subject to modification due to changes in knowledge and changes in conservation status. Publication reference: Latch, P. 2007. National recovery plan for the southern cassowary Casuarius casuarius johnsonii . Report to Department of the Environment, Water, Heritage and the Arts, Canberra. Environmental Protection Agency. 2 Contents Executive Summary................................................................................................................... 4 1. General information.............................................................................................................. -
Molecular Identification of Gambierdiscus and Fukuyoa
marine drugs Short Note Molecular Identification of Gambierdiscus and Fukuyoa (Dinophyceae) from Environmental Samples Kirsty F. Smith 1,*, Laura Biessy 1, Phoebe A. Argyle 1,2, Tom Trnski 3, Tuikolongahau Halafihi 4 and Lesley L. Rhodes 1 1 Coastal & Freshwater Group, Cawthron Institute, Private Bag 2, 98 Halifax Street East, Nelson 7042, New Zealand; [email protected] (L.B.); [email protected] (P.A.A.); [email protected] (L.L.R.) 2 School of Biological Sciences, University of Canterbury, Private Bag 4800, 20 Kirkwood Avenue, Christchurch 8041, New Zealand 3 Auckland War Memorial Museum, Private Bag 92018, Victoria Street West, Auckland 1142, New Zealand; [email protected] 4 Ministry of Fisheries, P.O. Box 871, Nuku’alofa, Tongatapu, Tonga; [email protected] * Correspondence: [email protected]; Tel.: +64-3-548-2319 Received: 30 March 2017; Accepted: 28 July 2017; Published: 2 August 2017 Abstract: Ciguatera Fish Poisoning (CFP) is increasing across the Pacific and the distribution of the causative dinoflagellates appears to be expanding. Subtle differences in thecal plate morphology are used to distinguish dinoflagellate species, which are difficult to determine using light microscopy. For these reasons we sought to develop a Quantitative PCR assay that would detect all species from both Gambierdiscus and Fukuyoa genera in order to rapidly screen environmental samples for potentially toxic species. Additionally, a specific assay for F. paulensis was developed as this species is of concern in New Zealand coastal waters. Using the assays we analyzed 31 samples from three locations around New Zealand and the Kingdom of Tonga.