Mycoremediation Applications for Stormwater Management

Total Page:16

File Type:pdf, Size:1020Kb

Mycoremediation Applications for Stormwater Management Mycoremediation Applications for Stormwater Management Design Challenge: Airport Environmental Interactions; Innovative methods for stormwater management at airports Team Members (All Undergraduate): Gray Bender, Austin Bennett, Kevin Cooper, Melanie Dickison, Leopold Kisielinski, Erica Wiener University: University of Colorado, Boulder Advisor: Christopher Corwin, PhD Table of Contents Executive Summary ........................................................................................................................ 4 1.0 Problem Statement and Background .................................................................................... 5 2.0 Problem Solving and Design Approach ............................................................................... 7 3.0 Summaries of Literature Review ......................................................................................... 9 3.1 Bioretention ...................................................................................................................... 9 3.1.1 Background ................................................................................................................... 9 3.1.2 Case Studies ................................................................................................................ 11 3.2 Chemical Addition ......................................................................................................... 15 3.2.1 Background ................................................................................................................. 15 3.2.2 Case Studies ................................................................................................................ 17 3.3 Mycoremediation ........................................................................................................... 18 3.3.1 Background ................................................................................................................. 18 3.3.2 Case Studies ................................................................................................................ 19 4.0 Safety Risk Assessment and Regulatory Evaluation ......................................................... 21 5.0 Technical Description ........................................................................................................ 22 5.1 Bench-Scale Treatability Study ...................................................................................... 22 5.1.1 Substrates .................................................................................................................... 23 5.1.2 Filter Preparation ........................................................................................................ 24 5.2 Pilot-Scale Test .............................................................................................................. 26 5.3 Monitoring ...................................................................................................................... 27 5.4 Maintenance ................................................................................................................... 28 6.0 Airport Interactions ............................................................................................................ 29 7.0 Immediate Impacts ............................................................................................................. 29 7.1 Financial Analysis .......................................................................................................... 29 8.0 Future Impacts ................................................................................................................... 31 8.1 Commercial Potential ..................................................................................................... 31 8.1.1 Airplane Fuel .............................................................................................................. 31 8.1.2 Airplane Deicing Fluid ............................................................................................... 32 8.1.3 Assisting Revegetation ............................................................................................... 33 8.2 Social/Environmental Impacts ....................................................................................... 33 i 9.0 Conclusion ......................................................................................................................... 34 Appendix A: Team Contact Information .................................................................................... A-1 Team Members ....................................................................................................................... A-1 Advisor .................................................................................................................................... A-2 Appendix B: Description of University ...................................................................................... B-1 Appendix C: Description of Non-University Partners ................................................................ C-1 DIA ......................................................................................................................................... C-1 Fungi Perfecti .......................................................................................................................... C-1 Marc Beutel ............................................................................................................................. C-1 Appendix D: Sign-Off Forms ..................................................................................................... D-1 Appendix E: Evaluation of Educational Experience ................................................................... E-1 Students .................................................................................................................................... E-1 Faculty...................................................................................................................................... E-3 Appendix F: References ............................................................................................................... F-1 List of Figures Figure 1: View of Pond 927/Outfall A. .......................................................................................... 6 Figure 2: Photo of concrete cutting on a runway. ........................................................................... 7 Figure 3: Cost versus MCDA Score for each alternative. .............................................................. 9 Figure 4: Cross-sectional diagram of a detention system. ........................................................... 11 Figure 5: Concentration changes from inflow to outflow of specific pollutants. ......................... 12 Figure 6: Graph of bypass volume vs amount of rainfall for each precipitation event. ............... 13 Figure 7: Warm condition data compared to cold conditions ...................................................... 14 Figure 8: Concentrations changes from inflow to outflow of specific pollutants ........................ 15 Figure 9: Standard bubbler system. .............................................................................................. 16 Figure 10: A proposed pathway for the degradation of lignin using MnP. ................................. 19 Figure 11: Results of resiliency testing ......................................................................................... 23 Figure 12: Picture of visible mycelium within the mycofilter bucket. ......................................... 25 ii List of Tables Table 1: MCDA template used to rate alternatives. ........................................................................ 8 Table 2: Specific pollutants and the removal mechanisms ........................................................... 10 Table 3: pH measurements for different substrates ...................................................................... 20 Table 4: Different substrate combinations in the 2012 study. ...................................................... 23 Table 5: Proposed fungal species and substrate combinations for the bench-scale study ............ 24 Table 6: Bench-Scale Study Outcomes......................................................................................... 26 Table 7: Cost of Mycoremediation ............................................................................................... 30 Abbreviations ACRP – Airport Cooperative Research Program ADF – Aircraft Deicing Fluid BMP – Best Management Practice CDPHE – Colorado Department of Public Health and Environment DIA – Denver International Airport EMS – Environmental Management System EPA – Environmental Protection Agency LiP – Lignin Peroxidase MCDA – Multiple Criteria Decision Analysis MnP – Manganese Peroxidase NPDES – National Pollutant Discharge Elimination System Stormwater Program PAH – Polycyclic Aromatic Hydrocarbons PCB – Polychlorinated Biphenyls SMS – Spent Mushroom Substrate SSC – Suspended Sediment Concentration S3 – Sustainable Stormwater Solutions SWMP – Stormwater Management Plan TPH – Total Petroleum Hydrocarbons TSS – Total Suspended Solids TWG – Targeted Watershed Grant WSU – Washington State University iii Executive Summary Sustainable Stormwater Solutions (S3) is a group of six undergraduate engineering students from the University of Colorado, Boulder. In order to participate in the Airport Cooperative Research Program (ACRP) University
Recommended publications
  • Mycoremediation of Hexavalent Chromium by Macrofungi
    International Journal of Chemical Studies 2019; 7(3): 2777-2781 P-ISSN: 2349–8528 E-ISSN: 2321–4902 IJCS 2019; 7(3): 2777-2781 Mycoremediation of hexavalent chromium by © 2019 IJCS Received: 22-03-2019 macrofungi: A novel approach Accepted: 24-04-2019 Mownitha V Mownitha V, Avudainayagam S, Sara Parwin Banu K and Department of Environmental Krishnamoorthy AS Sciences, TNAU, Coimbatore, Tamil Nadu, India Abstract Avudainayagam S In the present study the biosorption efficiency of macrofungal cultures (Pleurotus florida, Pleurotus Department of Environmental eous, Hypsizygus ulmarius, Schizophyllum sp., Coprinus sp. and Ganoderma lucidum) in the removal of Sciences, TNAU, Coimbatore, hexavalent chromium (Cr (VI)) from aqueous solution was being explored. The influence of parameters Tamil Nadu, India like initial Cr (VI) concentration, macrofungal culture and contact time were studied. From the results, it was evident that maximum biosorption of Cr (VI) was observed in Schizophyllum sp. (86.24%) and Sara Parwin Banu K Ganoderma lucidum (83.97%) at the Cr (VI) concentration and contact time of 75 mg/L and 15 days Department of Environmental Sciences, TNAU, Coimbatore, respectively. The FTIR spectra of the fungal biomass before and after Cr (VI) biosorption confirmed the Tamil Nadu, India presence of functional groups and their involvement in the biosorption process. Hence, Schizophyllum sp. and Ganoderma lucidum can be utilized as a Mycoremediation tool in removing Cr (VI) from Krishnamoorthy AS contaminated water. Department of Plant Pathology, TNAU, Coimbatore, Keywords: Cr (VI), biosorption, macrofungal cultures, mycoremediation Tamil Nadu, India Introduction Heavy metal pollution is an overarching environmental problem which we are facing today.
    [Show full text]
  • Mycoremediation by Oyster Mushroom
    Acta Scientific AGRICULTURE (ISSN: 2581-365X) Volume 5 Issue 5 May 2021 Perspective Mycoremediation by Oyster Mushroom Nidhi Akkin* Received: March 15, 2021 Plant Pathology, University of Agricultural Sciences Bangalore, Bangalore, Published: April 16, 2021 Karnataka, India © All rights are reserved by Nidhi Akkin. *Corresponding Author: Nidhi Akkin, Plant Pathology, University of Agricultural Sciences Bangalore, Bangalore, Karnataka, India. Oyster mushroom is the Pleurotus species, which is a well- parts, it is found that different species have different accumulation known fungus that can be used in bioremediation of the soil con- capacity for various metals like copper, zinc, molybdenum, manga- taminated by pesticides and heavy metals in ecosystem. Cultiva- nese and iron. When Pleurotus sajor-caju is grown on duckweed tion of this mushroom is an age-old practice. Though the biological which is rich in metals elements, it is found that there is accumula- remediation properties were known from a very long time, as long tion of cadmium ions above the permissible limits. Concentration as the period of world war one, but only a little was done to com- of mercury and cadmium is found to be high in basidium of P. os- mercialize it or incorporate it in our daily lives. The absorption po- treatus when grown in polluted areas. The uptake of copper and tential of Pleurotus species is still to be known to the fullest extent. cadmium ions is higher as compared to cobalt and mercuric ions by P. sajor-caju. Decomposition rate of DDVP in the soil was high in Mycoremediation is a method of clarifying heavy metals by the presence of Pleurotus pulmonarius.
    [Show full text]
  • Evaluation of Fungcoal As a Bioprocess Technology for Self-Cladding of Waste Coal Dumps
    Evaluation of Fungcoal as a bioprocess technology for self-cladding of waste coal dumps A thesis submitted in fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY (Environmental Biotechnology) of Rhodes University By Lerato M. Sekhohola FEBRUARY 2015 1 Abstract Low-grade coal, a poor source of energy, has long been regarded as waste material by the coal mining industry. Biological degradation of this coal material by ligninolytic fungal strains presents a viable strategy towards eliminating this unusable fossil fuel. To this end, a novel and patented bioprocess termed Fungcoal was developed. Fungcoal is a biological process utilised in the in situ treatment of waste coal and is based on the mutualistic relationship between the fungus Neosartorya fischeri and the graminaceous species Cynodon dactylon. The process facilitates the rapid conversion of waste coal into soil-like material that stimulates establishment of vegetation for eventual coal dump rehabilitation. While a number of in vitro studies have identified various fungal strains as efficient coal degraders, the mechanisms involved in the Fungcoal-stimulated degradation process have not been fully elucidated. Furthermore, implementation of Fungcoal at both pilot and commercial scale has not been achieved. Thus the objective of this work was to investigate Fungcoal as a bioprocess via examining the role of coal degrading fungi (CDF) and grasses as biocatalysts in coal biodegradation and for the self-cladding of waste coal dumps. Initially, waste coal degradation by N. fischeri, strain ECCN 84, was investigated, specifically focusing on the mechanisms underpinning the process. In vitro studies showed the addition of waste coal induced active fungal colonisation resulting in increased fungal biomass.
    [Show full text]
  • Glossary of Terms for Water Solutions: Bernheim Forest
    Glossary of Terms for Water Solutions: Bernheim Forest Algae bloom is an explosion of algae growth in a body of water due to an excess of nutrients in the water from fertilizer or organic waste pollution. The decomposition of dead algae can cause a shortage of oxygen in the water leading to the death of other water organisms. A “fish kill” is a good example of this process. Cisterns are underground tanks or containers used to hold water. At the Bernheim Forest Visitor Center, cisterns collect rainwater from the green roof that is subsequently used to flush the Center’s toilets. Channelization is the process of channelizing a stream, usually a gradual process that straightened the stream’s path by removing its meanders. It improved the landowner’s access to fertile bottomland and/or made it easier to navigate the stream. Channelization harms the health of the stream and the surrounding riparian zone and increases non-point-source pollution and flooding downstream. At Bernheim, the channelization of Wilson Creek was reversed, and the stream was restored to its natural meanders. Cobbles are rocks that have been rounded by wave action or running water. These rocks pile up to form riffles or shallow areas in meandering streams. Contaminants are impurities or substances that pollute other substances. In fact, pollution is defined as the introduction of contaminants into an environment that have negative impacts on the ecosystem to which they have been introduced. Detached wetlands are small temporary pools that form in the bottomlands of streams after flooding. They allow water to slow down and soak slowly into the watershed.
    [Show full text]
  • Ecotoxicity of Soil Contaminated with Diesel Fuel and Biodiesel Małgorzata Hawrot‑Paw 1*, Adam Koniuszy1, Grzegorz Zając2 & Joanna Szyszlak‑Bargłowicz2
    www.nature.com/scientificreports OPEN Ecotoxicity of soil contaminated with diesel fuel and biodiesel Małgorzata Hawrot‑Paw 1*, Adam Koniuszy1, Grzegorz Zając2 & Joanna Szyszlak‑Bargłowicz2 Fuels and their components accumulate in soil, and many soil organisms are exposed to this pollution. Compared to intensive research on the efect of conventional fuel on soil, very few studies have been conducted on soil ecotoxicity of biofuels. Considering the limited information available, the present study evaluated the changes caused by the presence of biodiesel and diesel fuel in soil. The reaction of higher plants and soil organisms (microbial communities and invertebrates) was analysed. Conventional diesel oil and two types of biodiesel (commercial and laboratory‑made) were introduced into the soil. Two levels of contamination were applied—5 and 15% (w/w per dry matter of soil). The plate method was used to enumerate microorganisms from soil contaminated with biodiesel and diesel fuel. Phytotoxicity tests were conducted by a 3‑day bioassay based on the seed germination and root growth of higher plant species (Sorghum saccharatum and Sinapis alba). Fourteen‑day ecotoxicity tests on earthworm were performed using Eisenia fetida. Based on the results of the conducted tests it was found out that the organisms reacted to the presence of fuels in a diverse manner. As to the microorganisms, both the growth and reduction of their number were noted. The reaction depended on the group of microorganisms, type of fuel and dose of contamination. The lipolytic and amylolytic microorganisms as well as Pseudomonas fuorescens bacteria were particularly sensitive to the presence of fuels, especially biodiesel.
    [Show full text]
  • Mycoremediation of Polycyclic Aromatic Hydrocarbons (PAH)-Contaminated Oil-Based Drill-Cuttings
    African Journal of Biotechnology Vol. 10(26), pp. 5149-5156, 13 June, 2011 Available online at http://www.academicjournals.org/AJB DOI: 10.5897/AJB10.1108 ISSN 1684–5315 © 2011 Academic Journals Full Length Research Paper Mycoremediation of polycyclic aromatic hydrocarbons (PAH)-contaminated oil-based drill-cuttings Reuben N. Okparanma*, Josiah M. Ayotamuno, Davidson D. Davis and Mary Allagoa Department of Agricultural and Environmental Engineering, Rivers State University of Science and Technology, Port Harcourt, P.M.B 5080, Rivers State, Nigeria. Accepted 4 March, 2011 Spent white-rot fungi (Pleurotus ostreatus) substrate has been used to biotreat Nigerian oil-based drill cuttings containing polycyclic aromatic hydrocarbons (PAHs) under laboratory conditions. The Latin square (LS) experimental design was adopted in which four options of different treatment levels were tested in 10 L plastic reactors containing fixed masses of the drill cuttings and fresh top-soil inoculated with varying masses of the spent P. ostreatus substrate. Each option was replicated three times and watered every 3 days under ambient conditions for a period of 56 days. Microcosm analysis with a series II model 5890 AGILENT Hp® GC-FID showed that, the PAHs in the drill cuttings were mainly composed of 2 to 5 fused rings with molecular-mass ranging from 128 to 278 g/mol, while the total initial PAHs concentration of the drill cuttings was 806.31 mg/kg. After 56 days of composting, the total amount of residual PAHs in the drill cuttings decreased to between 19.75 and 7.62%, while the overall degradation of PAHs increased to between 80.25 and 92.38% with increasing substrate addition.
    [Show full text]
  • Mycoremediation of Environmental Pollutants
    International Journal of ChemTech Research CODEN (USA): IJCRGG, ISSN: 0974-4290, ISSN(Online):2455-9555 Vol.10 No.3, pp 149-155, 2017 Mycoremediation of Environmental Pollutants Ved Prakash* Department of Biotechnology College of Engineering & Technology, IILM Academy of Higher Learning, Greater Noida, India Abstract : A wide number of fungal species have shown incredible abilities to degrade a growing list of persistent and toxic industrial waste products and chemical contaminants to less toxic form or non-toxic form. Mycelium reduces toxins by different enzymatic mechanism to restore the natural flora and fauna. White rot fungi has successfully been utilized in degradation of environmental pollutant like polyaromatic compounds, pesticides etc. The present review gives a insights on degradation aspects of heavy metals, PAH especially using different fungal species. White rot fungi has potential to degrade contaminants using wide range of enzymes. Mycoremediation is promising alternative to replace or supplement present treatment processes. Keywords: Mycoremediation, Heavy metal, PAH, White rot fungi, Contaminant. Introduction Recent rapid and progressive development of technology and industry has led to increasing the proportion of various environmental pollutants, such as pesticides, toxic xenobiotic, metals, metalloids and halogenated and polycyclic aromatic hydrocarbons1. Microbial remediation of metal is a complex process that depends on the chemistry of metal ions, cell wall composition of microorganism, cell physiology, and physic- chemical factors like pH, temperature, time, ionic strength and metal concentration2. The retention time of metals in soil is thousands of years because, unlike organic pollutant, metals are not degraded biologically. They rather are transformed from one oxidation state or organic complex into another and therefore persist in soil3.
    [Show full text]
  • Comprehensive Genomic and Transcriptomic Analysis Of
    1 Comprehensive Genomic and Transcriptomic Analysis of 2 Polycyclic Aromatic Hydrocarbon Degradation by a 3 Mycoremediation Fungus, Dentipellis sp. KUC8613 4 5 Hongjae Parka, Byoungnam Minb, Yeongseon Jangc, Jungyeon Kimj, Anna 6 Lipzenb, Aditi Sharmab, Bill Andreopoulosb, Jenifer Johnsonb, Robert Rileyb, 7 Joseph W. Spataforad, Bernard Henrissate,f,g, Kyoung Heon Kimj, Igor V. 8 Grigorievb, i, Jae-Jin Kimh, and In-Geol Choi1,* 9 10 aDepartment of Biotechnology, College of Life Sciences and Biotechnology, 11 Korea University, Seoul 02841, Korea, bUS Department of Energy Joint 12 Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, United 13 State, cDsivision of Wood Chemistry & Microbiology, Korea Forest Research 14 Institute, Seoul, 130-712, Korea, dDepartment of Botany and Plant 15 Pathology, Oregon State University, Corvallis, OR 97331-2902, United 16 States, eArchitecture et Fonction des Macromolécules Biologiques, Centre 17 National de la Recherche, France, fScientifique, Université d'Aix-Marseille, 18 France, Institut National de la Recherche Agronomique, USC 1408 AFMB, 19 Marseille, France, gDepartment of Biological Sciences, King Abdulaziz 20 University, Jeddah, Saudi Arabia, hDivision of Environmental Science and 21 Ecological Engineering, College of Life Sciences and Biotechnology, Korea 22 University, Seoul 02841, iDepartment of Plant and Microbial Biology, 23 University of California - Berkeley, Berkeley, 111 Koshland Hall, Berkeley, 24 CA 94720, jDepartment of Biotechnology, Graduate School, Korea 25 University, Seoul, 02841, South Korea 26 27 28 * Corresponding author: 29 In-Geol Choi 30 Tel.: +82-2-3290-3152, E-mail address: [email protected] 31 32 2 33 Abstract 34 The environmental accumulation of polycyclic aromatic 35 hydrocarbons (PAHs) is of great concern due to potential carcinogenic and 36 mutagenic risks, as well as their resistance to remediation.
    [Show full text]
  • MYCOREMEDIATION of SOIL CONTAMINATED with CADMIUM and LEAD by Trichoderma Sp
    DOI: 10.2478/eces-2021-0020 ECOL CHEM ENG S. 2021;28(2):277-286 Katarzyna BANDURSKA 1*, Piotr KRUPA 1, Agnieszka BERDOWSKA 1 Igor JATULEWICZ 1 and Iwona ZAWIERUCHA 1 MYCOREMEDIATION OF SOIL CONTAMINATED WITH CADMIUM AND LEAD BY Trichoderma sp. Abstract: Conducted research determined the effect of the Trichoderma fungi on the presence of cadmium and lead ions in the soil contaminated by mentioned elements. The aim of the study was to demonstrate whether the fungi of this kind can contribute to remediation of soil by the immobilization of heavy metals. Experiments were conducted in laboratory conditions. The vaccine containing spores of Trichoderma asperellum was introduced into the soil contaminated with cadmium and lead by direct injection. Analyses of the soluble fraction of selected heavy metals were performed after 3 and 15 days of cultivation using atomic absorption spectrometry (AAS). Statistical significant positive effects on the immobilization of lead ions and no statistical differences in inhibition of cadmium translocation were observed. The results showed that Trichoderma fungi are suited to support the process of environment remediation by removal of lead. This suggests possible application of Trichoderma asperellum in mycoremediation and supporting role in phytoremediation of soil. Keywords: mycoremediation, Trichoderma sp., heavy metals, biological plant protection Introduction Natural environment is balanced by the circulation of chemical elements. However, the existing stability between its release and re-bond in the geological formations is often disturbed by human activity. Due to combustion of coals, liquid fuels, substances and raw material processing, large amounts of gaseous pollutants and dust are introduced into the atmosphere, soil and water.
    [Show full text]
  • Mycoremediation of Pcbs by Pleurotus Ostreatus: Possibilities and Prospects
    applied sciences Review Mycoremediation of PCBs by Pleurotus ostreatus: Possibilities and Prospects Se Chul Chun 1 , Manikandan Muthu 1, Nazim Hasan 2, Shadma Tasneem 2 and Judy Gopal 1,* 1 Department of Environmental Health Sciences, Konkuk University, Seoul 143-701, Korea; [email protected] (S.C.C.); [email protected] (M.M.) 2 Department of Chemistry, Faculty of Science, Jazan University, Jazan, P.O. Box 114, Saudi Arabia; [email protected] (N.H.); [email protected] (S.T.) * Correspondence: [email protected]; Tel.: +82-2450-0574; Fax: +82-2450-3310 Received: 22 August 2019; Accepted: 21 September 2019; Published: 8 October 2019 Abstract: With the rising awareness on environmental issues and the increasing risks through industrial development, clean up remediation measures have become the need of the hour. Bioremediation has become increasingly popular owing to its environmentally friendly approaches and cost effectiveness. Polychlorinated biphenyls (PCBs) are an alarming threat to human welfare as well as the environment. They top the list of hazardous xenobiotics. The multiple effects these compounds render to the niche is not unassessed. Bioremediation does appear promising, with myco remediation having a clear edge over bacterial remediation. In the following review, the inputs of white-rot fungi in PCB remediation are examined and the lacunae in the practical application of this versatile technology highlighted. The unique abilities of Pleurotus ostreatus and its deliverables with respect to removal of PCBs are presented. The need for improvising P. ostreatus-mediated remediation is emphasized. Keywords: mycoremediation; PCBs; Pleurotus; xenobiotics; fungus 1. Introduction The synthetic compounds obtained through chlorination of biphenyls are called polychlorinated biphenyls (PCBs), which are composed of a biphenyl molecule (two benzene rings linked by a C–C bond) that carries one to ten chlorine atoms.
    [Show full text]
  • Biodegradation of Polycyclic Aromatic Hydrocarbons in Petroleum Oil Contaminating the Environment
    Aim of the Work BIODEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN PETROLEUM OIL CONTAMINATING THE ENVIRONMENT Presented by Abir Moawad Partila A Thesis Submitted to Faculty of Science In Partial Fulfillment of the Requirements for the Degree of Ph.D. of Science (Microbiology) Botany Department Faculty of Science Cairo University (2013) i Aim of the Work ABSTRACT Student Name: Abir Moawad Partila Girgis Title of the thesis: Biodegradation of Polycyclic Aromatic Hydrocarbon in petroleum oil contaminating the environment Degree: Ph. D. (Microbioliogy) Soil and sludge samples polluted with petroleum waste from Cairo Oil Refining Company Mostorod, El-Qalyubiah, Egypt for more than 41 years were used for isolation of indigenous microbial communities. These communities were grown on seven polycyclic aromatic hydrocarbon compounds .Six isolates (MAM-26, 29, 43, 62, 68, 78) were able to grow on different concentrations of five chosen PAHs. The best degraders bacterial isolates MAM-29 and MAM-62 were identified by 16S-rRNA. As Achromobacterxylosoxidans and Bacillus amyloliqueficiensrespectively.The most promising bacterial strain Bacillus amyloliqueficiens have been exposed to different doses of gamma radiation to improve its qualities. Keywords: Polycyclic- Aromatic- Hydrocarbon – Biodegradation-Pollution. Supervisors: Signature 1- Prof. Dr. Youssry Saleh 2-Prof. Dr. Mervat Aly Abou-State Prof. Dr. Gamal Fahmy Chairman of Botany Department Faculty of Science-Cairo University ii Aim of the Work APROVAL SHEET FOR SUMISSION Thesis Title: Biodegradation of Polycyclic Aromatic Hydrocarbons In Petroleum Oil Contaminating The Environment Name of candidate: Abir Moawad Partila This thesis has been approved for submission by the supervisors: 1- Prof. Dr. Youssry Saleh Signature: 2- Prof.
    [Show full text]
  • Vermiremediation and Mycoremediation of Polycyclic Aromatic Hydrocarbons in Soil and Sewage Sludge Mixture: a Comparative Study
    International Journal of Environmental Science and Development, Vol. 4, No. 5, October 2013 Vermiremediation and Mycoremediation of Polycyclic Aromatic Hydrocarbons in Soil and Sewage Sludge Mixture: A Comparative Study A. B. Azizi, K. Y. Liew, Z. M. Noor, and Noorlidah Abdullah unburned fuel absorbed into street dust [4]. Abstract—Polycyclic aromatic hydrocarbons-contaminated Research on the potential utilisation of earthworms has soil and sewage sludge mixtures in microcosms were tested shown an ability to manage polluted land and even sewage separately in triplicate with two types of biological agents, sludge, which is termed as vermicomposting or namely earthworms (Lumbricus rubellus) and spent fungal vermistabilisation. In its basic form, this is a low-cost mycelia of Pleurotus sajor-caju (SPC) for 60 days. The results indicated that vermiremediation (VMR) and mycoremediation technology system that primarily uses earthworms in the (MYC) showed degradation result of 99.99% PAHs i.e. Anth, processing or treatment of organic wastes. In this field, Phen and BaP removal. Ten earthworms and 750 g of SPC application of Lumbricus rubellus is scarce compare to depicted optimum results in nutrient element content among Eisenia foetida. Apart from the role of earthworms in respective treatments. Treatment of MYC resulted a bioremediation, white-rot fungi also potential in producing consistence increment of soil macronutrients except for TP and extracellular enzymes, which degrade PAHs. Similar TKN (500 g treatment) compared to VMR. Hence, sixty days incubation of spent fungal mycelia of Pleurotus sajor-caju scenario reported for Pleurotus sajor-caju, which not compost (SPC) is potential to degrade 99.99% of Anth, Phen commonly tested on its ability to degrade persistence organic and BaP but at the same time enrich the soil macronutrients i.e.
    [Show full text]