DOCSLIB.ORG

Histology of the Testicle, Spermatogenesis

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Histology of the Testicle, Spermatogenesis Histology of the testicle, spermatogenesis Dr. Zsuzsanna Tóth Semmelweis University Department of Anatomy, Histology and Embryology The structure of the testis lobule GE Lu interstitium Trichrom staining Seminiferous convoluted tubules: • germinal (spermatogenic) epithelium; Sertoli cells, developing germinal cells • basal lamina • myoid cells • connective tissue wall H & E Lu GE Sp GE Leydig cell Myoid cells Intersitial Leydig cells produce testosterone • Form groups (10-20 cells) • Steroid producing cell characteristics: polygonal shape, acidophylic cytoplasm, round nucleus with nucleolus, abundant SER, lipid dropplets in the cytoplasm • Cytoplasmatic hexagonal prisms: Reinke crystalloids • The number of the Leydig cells decreases in erderly • Regulation by the pituitary gland, Luteinizing-hormone (LH) – stimulatory The role of testosterone in sexual differentiation SRY gene: sex-determining region of the Y chromosome) Types of cell division redukció Generation of genetic diversity in meiosis I. • Genetic variation: homologous recombination random segregation of the chromosomes Meiosis II is similar to mitosis • Meiosis II : starts without DNA replication • Produces four haploid daughter cells: • male: 4 sperms, 22+X or 22+Y chromosome sets • female: 1 ovum and 3 polar bodies (22+X) Spermatogenesis • Spermatogonia are of endodermal (4th week, yolk sac) origin • Spermatogenic syntitium: sister cells are connected by bridges of cytoplasm (intense cell-cell communication) 64 • The grouped cells develop days synchronously • Meiosis starts at the puberty and then it takes place continously • Follicle stimulating hormone (FSH) have stimulatory effect The Sertoli cell Supporting and nutrient function Cheng C Y , and Mruk D D Physiol Rev 2002;82:825-874 • Columnar epithelial cells, develop from the epithelial sex cords AdluminalAdluminalis • Span from the basement membrane to the lumen compartmentcompartment • Ovoid, triangular cell nucleus, pale nuclear staining, the nucleolus is visible • Intercellular pockets - nursery for the proliferating germinal cells Blood Vér- testis-here barriergát • Tight junctions between Sertoli cells: basal and adluminal compartments: Blood testis barrier( avascular parenchyma, protection against autoimmunity) • Phagocytic activity BasalisBasal • Secrete testicular fluid, androgen-binding protein, inhibin compartment compartment • Anti-mullerian hormone secretion during embryonic development The blood-testis barrier defines the adluminal and basal compartments Cheng C Y , and Mruk D D Physiol Rev 2002;82:825-874 ©2002 by American Physiological Society Spermatogenesis waves move in spirals Groupings of various generations in a tubule cross-section: spermatogonia spermatocytes spermatids seminiferous tubule Morphology of developing germinal cells Spermatogonia: are in contact with the basal lamina, have medium sized, round nucleus Primary spermatocytes (MI): are the largest cells, with large round nucleus, visible chromosomes Secondary spermatocytes (MII): are smaller, have very short lifetime, rarely seen Round and elongated spermatides: are small with condensed (dark) nuclei in the innermost layer Spermatozoa: head and tail, are seen in the lumen Sertoli cells: large cells, ovoid, pale nucleus with nucleolus Spermiogenesis: maturation of the spermatids into spermatozoa • Development of motility: flagellum formation • Size reduction: Nuclear condensation: the DNA is tightly packed, most of the RNA is eliminated Reduction of cytoplasmatic mass • Formation of the acrosome: a cell organelle, with enzymes necessary to fertilization of the ovum • Energy: many mitochondria (ATP) The structure of the sperm cell human spermatozoa (~60 µm) Acrosome (contains enzymes necessary for penetration into the ovum) Head nucleus cell membrane mitochondria Neck centrioles axoneme Mid (connecting) microtubule complex, outer dense fibres dense fibers piece spiral mitochondrial complex cell membrane longitudinal column outer dense axial filament of the fibrous sheath Tail fibres circumferential axoneme (9+2 pairs of MT) Principal ribs of the FS cell longitudinal column piece microtubules (MT), membrane of the fibrous sheath dense fibres axoneme (9 pairs of outer microtubules) cell membrane axoneme (central pair of inner microtubules) End piece microtubules cell membrane Motility is only activated during ejaculation. Step by step spermiogenesis 1. Golgi phase: proacrosomal granules pinch off from Golgi; primitive flagellum formation (axoname) starts from the periferal centriole at the opposite pole of the cell. 2. Cap phase: granules fuse (PAS+) and form the acrosomal cap that associates with the rostral nuclear envelope, chromatin condensation starts, the flagellum grows 3. Acrosomal phase: further chromatin condensation, excentric nucleus with closly adhered acrosome, elongation, the peripheral centriole forms 9 dense fibres and the fibrous sheeths around the axoname. Spiral mitochondria form. 4. Maturation phase (spermiation): formation of the residual body (RB, excess cytoplasm) – Sertoli cells phagocyte the RBs, cytoplasmatic bridges disappear, free sperms enter the lumen Straight tubules (tubuli seminiferi recti) • radiate from the mediastinum, within the septa • transfer speratozoa from the seminiferous tubules toward the rete testis • epithelium: proximally single columnar (Sertoli) cells, distally cuboidal cells Rete testis • labyrinth like network of anastomosing channels embedded in the fibrous tissue of the mediastinum • flattened squamous, or low cuboidal epithelium lining, microvilli • mixes, transports and modifies the luminar fluid • drains into the efferent ductules Regulation of the testicular hormone production Influencing factors: • temperature • stress • inflammation (mumps) • ischaemia • radiation • cytotoxic drugs (chemotherapy).
Load more

Recommended publications
  • Epididymo-Orchitis
    Epididymo-orchitis In men over the age of 35 years the most Epididymo-orchitis Bladder common cause is a urine infection – with local Seminal spread of infection from the bladder. This may Epidiymo-orchitis – the basics vesicle Epididymo-orchitisIt is a condition- the basics affecting men characterised by also occur after surgical procedures such as pain and swelling inside the scrotum (ball bag) Prostate Rectum cystoscopy or catheterisation. Epididymo-orchitisand is duea tocondition an infection eitherthat in causesthe: pain and Urethra Occasionally it may also be due to a ‘gut’ swelling inside the scrotum (ball bag). epididymis – tube carrying the sperm from bacterial infection from insertive anal Te s t i s the testicle to the vas deferens and then the intercourse. It is due to an infectionurethra either or water in pipe the: (epididymitis) Rarely epididymo-orchitis may be caused by Penis • epididymistesticle – tube (orchitis) carrying the sperm from the other infections such as mumps or tuberculosis. testicle to theepididymis vas deferensand testicle (epididymo-orchitis)and then the Vas urethra or water pipe (epididymitis) deferens What would I notice if I had epididymo-orchitis? • In men under the age of 35 years it is usually A rapid onset of pain and swelling in one or testicle (orchitis) Epididymis caused by a sexually transmitted infection (STI) sometimes both of your testicles. • epididymisin theand water testicle pipe e.g. (epididymo chlamydia or gonorrhoea.-orchitis) Scrotal Te s t i s Some men may also notice a discharge from Skin Prompt medical assessment is needed to the tip of the water pipe and/or pain on passing In people undermake 35 sure theyou don’t infection have a twisted is testicleoften sexually urine.
    [Show full text]
  • Vocabulario De Morfoloxía, Anatomía E Citoloxía Veterinaria
    Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) Servizo de Normalización Lingüística Universidade de Santiago de Compostela COLECCIÓN VOCABULARIOS TEMÁTICOS N.º 4 SERVIZO DE NORMALIZACIÓN LINGÜÍSTICA Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) 2008 UNIVERSIDADE DE SANTIAGO DE COMPOSTELA VOCABULARIO de morfoloxía, anatomía e citoloxía veterinaria : (galego-español- inglés) / coordinador Xusto A. Rodríguez Río, Servizo de Normalización Lingüística ; autores Matilde Lombardero Fernández ... [et al.]. – Santiago de Compostela : Universidade de Santiago de Compostela, Servizo de Publicacións e Intercambio Científico, 2008. – 369 p. ; 21 cm. – (Vocabularios temáticos ; 4). - D.L. C 2458-2008. – ISBN 978-84-9887-018-3 1.Medicina �������������������������������������������������������������������������veterinaria-Diccionarios�������������������������������������������������. 2.Galego (Lingua)-Glosarios, vocabularios, etc. políglotas. I.Lombardero Fernández, Matilde. II.Rodríguez Rio, Xusto A. coord. III. Universidade de Santiago de Compostela. Servizo de Normalización Lingüística, coord. IV.Universidade de Santiago de Compostela. Servizo de Publicacións e Intercambio Científico, ed. V.Serie. 591.4(038)=699=60=20 Coordinador Xusto A. Rodríguez Río (Área de Terminoloxía. Servizo de Normalización Lingüística. Universidade de Santiago de Compostela) Autoras/res Matilde Lombardero Fernández (doutora en Veterinaria e profesora do Departamento de Anatomía e Produción Animal.
    [Show full text]
  • Spermatogonia, Primary and Secondary Spermatocytes, and Early and Late Spermatids
    David (Michelangelo) MED316 REPRODUCTIVE SYSTEM AND DISORDERS HISTOLOGY OF THE MALE REPRODUCTIVE SYSTEM Histology of Testes and Spermatogenesis Dr. Sinan Özkavukcu Ankara University Faculty of Medicine Dept. of Histology – Embryology Lab Director - Center for Assisted Reproduction Male Reproductive System • Testes • Genital excurrent ducts • Ductuli efferentes • Ductus epididymis • Ductus deferens • Accessory sex glands • Seminal vesicles • Prostate • Bulbourethral glands • External genitalia • Penis • Scrotum Endocrine control of male reproduction • Onset of puberty • brain determines the timing of onset of puberty • crucial role in controlling sexual behavior and reproduction is played by the hypothalamus • Terminals of gonadotropin-releasing hormone (GnRH)-secreting neurons release their secretions in the median eminence and infundibulum, where they enter the hypophyseal portal system • GnRH is then driven to the anterior pituitary, precisely to the gonadotrophs, basophil-staining cells, which constitute 10%– 15% of anterior pituitary cells and are located throughout the entire anterior lobe • The gonadotrophs synthesize follicle-stimulating hormone (FSH) and luteinizing hormone (LH) and release them into the systemic circulation; both hormones reach the testis by testicular arteries. An Introduction to Male Reproductive Medicine, Niederberger Anti-Müllerian Hormone in Disorders of Sex Determination and Differentiation Arq Bras Endocrinol Metab vol 49 nº 1 Fevereiro 2005 Circulation of testes • The arterial supply to the testes follows the lobular division of seminiferous tubules, • Each lobulus is supplied by one recurrent artery; segmental arteries and capillaries become branched between the Leydig cells and then give rise to the venous system. • The pattern of blood supply to the testis is also essential for maintaining a lower testicular temperature compared with body temperature.
    [Show full text]
  • Torsión Del Cordón Espermático
    Torsión del cordón espermático A. SííMí MoYÁNO, J. J. GÓMEZ Ruíz, A. GÓMEZ VEGAS, J. Bi.k’ouriz IzouínRDo, J. CORRAL Rosíu.o y L. RESEL EsrÉvEz Cátedra y Servicio de Urología. Hospital Universitario San Carlos. Universidad Complutense de Madrid La primera descripción de una torsión o vólvulo del cordón espermático parece que fue realizada por Delasiauve’, en el año 1840, bajo el siguiente epígrafe: «Necrosis de un testiculo ectópico ocasionado por una hernia inguinal estrangulada en el adulto». La torsión del cordón espermático con la consecuente isquemia e infarto hemorrágico del parénquima testicular constituye uno de los accidentesvasculares dídimo epididimarios más importantes y que, a pesar del aumento progresivo de su incidencia anual, obliga a la orquiectomia tanto o más que ninguna otra patología testicular, incluido lostumores de dicho órgano’3. Según se desprende de la literatura médica revisada, al igual que de nuestra propia experiencia, será difícil que disminuya ostensiblemente el número de exéresis testiculares por esta causa patológica en un futuro próximo, aun contando en el mayorde loscasos con la colaboración del paciente, nuevas técnicas para un diagnóstico precoz y una actuación de urgencia quirúrgica4- <‘L É2AÑ¡9 El error o la tardanza en diagnosticar este proceso agudo puede suponer la pérdida de la glándula testicular y por ello el médico general o pediatra, que son losque suelen inicialmenteobservara estospacientes, debenconocer la existencia de esta patología, su diagnóstico y tratamiento precoz. De todas formas, aunque la situación anatómica del testículo y su contenido permiten realizar una exhaustiva exploración física, desgraciadamente todavía la remota posibilidad de una torsión del cordón espermático queda muchas veces descartada del diagnóstico diferencial al no pensar en ella.
    [Show full text]
  • Information for Parents About Retractile Testicles
    Patient and Family Education Information for Parents About Retractile Testicles Your son has a retractile testicle. This is not dangerous, but there are some things you should know about this condition. How do testicles develop? During pregnancy, the testicles in boy babies actually grow inside Tunnel the abdominal cavity, not in the scrotum. Four months before Testicles birth, a tunnel formed by the smooth lining of the intestinal To scrotum cavity pushes down through the groin into the scrotum. Between 1-2 months before birth, the testicles move down through his tunnel to be anchored in the scrotum. As the tunnel moves through the abdominal muscles, it is wrapped with some of the muscle tissue. This muscle is called the cremaster muscle. When a boy is cold or nervous the cremaster muscle contracts, pulling the testicle up out of the scrotum and into the groin area. Testicles move through Is it dangerous for my son to have a retractile testicle? tunnel to Tunnel scrotum Closes In some boys, even though the testicle makes the complete trip into the scrotum, that testicle may move up and down along the tunnel. Some doctors believe that this is an exaggerated reflex response. Such testicles are called ‘retractile testes’ because they retract into the groin when the cremaster muscle contracts. This retraction can make it hard to find the testicle on examination. Sometimes it may be difficult to tell the difference between a retractile testicle and an undescended testicle (one that never made the full trip into the scrotum during development). There are significant differences between the two conditions.
    [Show full text]
  • Methodical Complex on Gross Anatomy for Ii Course
    MINISTRY OF HIGHER AND SECONDARY SPECIAL EDUCATION OF UZBEKISTAN BUKHARA STATE MEDICAL INSTITUTE NAMED AFTER ABU ALI IBN SINO DEPARTMENT OF ANATOMY "APPROVED" by Vice-Rector for Academic and educational work, Associate prof. G.J.Jarilkasinova ________________________________ "_____" ________________ 2020 Area of knowledge: 500000 - Health and social care Education field: 510000 - Healthcare Educational direction: 5510100 - Medical business 5111000 - Professional education (5510100 - Medicine business) 5510200 - Pediatric Medicine 5510300 - Medico-prophylactic business 5510400 – Dentistry (by directions) 5510900 – Medico-biological business EDUCATIONAL - METHODICAL COMPLEX ON GROSS ANATOMY FOR II COURSE Bukhara 2020 The scientific program was approved by the Resolution of the Coordination Council No. ___ of August ___, 2020 on the activities of educational and methodological associations in the areas of higher and secondary special and vocational education. The teaching and methodical complex was developed by order of the Ministry of Higher and Secondary Special Education of the Republic of Uzbekistan dated March 1, 2017 No. 107. Compilers: Radjabov A.B. - Head of the Department of Anatomy, Associate Professor Khasanova D.A. - Assistant of the Department of Anatomy, PhD Bobomurodov N.L. - Associate Professor of the Department of Anatomy Reviewers: Davronov R.D. - Head of the Department Histology and Medical biology, Associate Professor Djuraeva G.B. - Head of the Department of the Department of Pathological Anatomy and Judicial Medicine, Associate Professor The working educational program for anatomy is compiled on the basis of working educational curriculum and educational program for the areas of 5510100 - Medical business. This is discussed and approved at the department Protocol № ______ of "____" _______________2020 Head of the chair, associate professor: Radjabov A.B.
    [Show full text]
  • Anatomy and Physiology of a Bull's Reproductive Tract
    Beef Cattle Handbook BCH-2010 Product of Extension Beef Cattle Resource Committee Reproductive Tract Anatomy and Physiology of the Bull E. J. Turman, Animal Science Department Oklahoma State University T. D. Rich, Animal Science Department Oklahoma State University The reproductive tract of the bull consists of the testicles normally and usually produces enough sperm so that and secondary sex organs, which transport the sperma- the male will be of near normal fertility. However, since tozoa from the testicle and eventually deposits them in this condition appears to have a hereditary basis, such the female reproductive tract. These organs are the epi- males should not be used for breeding. If both testicles didymis, vas deferens and penis, plus three accessory are retained, the male will be sterile. sex glands, the seminal vesicles, prostate and Cowper’s Usually, hormone production is near normal in the gland. This basic anatomy is illustrated in figure 1 as a cryptorchid testicle and the male develops and behaves greatly simplified diagrammatic sketch. like a normal male. If the retained testicle is not The testicle has two very vital functions: (1) produc- removed at time of castration, the male will develop the ing the spermatozoa; and (2) producing the specific secondary sex characters of an uncastrated male. This male hormone, testosterone. The testicles are located operation is not as simple, nor as safe, as removing tes- outside of the body cavity in the scrotum. This is essen- ticles that are in the scrotum. Thus, it is recommended tial for normal sperm formation since this occurs only at to select against this trait by culling cryptorchid males.
    [Show full text]
  • Leydig Cell Tumor of the Testis
    Odaba ş et al. Eastern Journal of Medicine 3 (2): 78-79, 1998. Leydig cell tumor of the testis ODABAŞ Ö.1, DİLEK F.H.2, AVANOĞLU H.1, ATILLA M.K.1, YILMAZ Y.1, AYDIN S.1 Departments of Urology1 and Pathology School of Medicine, Yüzüncü Yıl University, Van Key words Testicle, Leydig cell tumor, adult had bilateral testicular Leydig cell tumor with adrenocortical adenoma and suggested to examine Introduction the adrenal gland in patients with testicular Leydig cell tumors (4). Adult patients may present with Leydig cell tumor is a rare form of testicular gynaecomastia, loss of libido, feminine hair neoplasm. It represents only 1 to 3 percent of all distribution and genital under-development. testicular tumors although it is the most common Testicular swelling is usually present, but where a form of the sex cord-mesenchyme tumors. The discrete tumor mass is not palpable , ultrasound is majority have been recognized in males between the extremely useful in confirming the presence of a ages of 20 and 60 years. However approximately one tumor (5). In our case, the patient admitted with fourth have been reported before puberty (1). tescular swelling as reported usually, not with The etiology of Leydig cell tumors is unknown. In hormonal disturbance. contrast to germ cell tumors, there is no correlation with cryptorchidism. Causing experimental production of Leydig cell tumor in mice following chronic estrogen administration or intrasplenic testicular autografting shows its hormonal basis (1). In addition, estrogen and progesterone receptors were detected in about 70 per cent of the Leydig tumor cells in an immunohistologic study, though no receptor was observed in normal Leydig cells (2).
    [Show full text]
  • THE UNIVERSITY of EDINBURGH
    THE UNIVERSITY of EDINBURGH Title Urethroscopy: an aid to diagnosis, treatment and prognosis of urethral conditions in the male due to gonorrhoea Author McFarlane, Wilfrid Qualification MD Year 1919 Thesis scanned from best copy available: may contain faint or blurred text, and/or cropped or missing pages. Digitisation Notes: • Page 8 of Supplement at back is missing Scanned as part of the PhD Thesis Digitisation project http://librarvblogs.is.ed.ac.uk/phddigitisation URETHROSCOPY an aid to Diagnosis, Treatment and Prognosis of Urethral Conditions in the Male due to GONORRHOEA. "by Wilfrid McFarlane, M.C., M.B., Ch.E. (Edin) L.R.C.P. & S.E. M.O. 9. Stationary Hospital, Havre. 1916 M.O. i/c Gonorrhoeal Division, Military Hospital, Hemel Hempstead. 1918 M.O. Venereal Hospital, Cambridge. Thesis for"the Degree of M.D. - f 1• THE ANATOMY AMD HISTOLOGY OF THE URETHRA AND THE PATHOLOGY OF GONORRHOEA. In order to make a correct diagnosis and to carry out a sound treatment of any disease it is essential to have an accurate knowledge of the anatomy of the l • organ affected and of the pathology of the disease affecting it. THE -ANATOMY OF THE MALE URETHRA. It is merely necessary to bring out those points which will enable one to understand the effect of Gonorrhoea on the urethra, especially in longstanding cases. The urethra is the channel by which urine passes from the bladder to the outside. Into this channel open the ejaculatory ducts and thus it acts also as a passage for the spermatic fluid. In its course from the neck of the bladder to the root of the penis the urethra describes a curve, the concavity of which looks upwards and forwards.
    [Show full text]
  • Eight Days of Water-Only Fasting Promotes Favorable Changes in the Functioning of the Urogenital System of Middle-Aged Healthy Men
    nutrients Article Eight Days of Water-Only Fasting Promotes Favorable Changes in the Functioning of the Urogenital System of Middle-Aged Healthy Men Sławomir Letkiewicz 1,2, Karol Pilis 1,* , Andrzej Sl˛ezak´ 1 , Anna Pilis 1, Wiesław Pilis 1, Małgorzata Zychowska˙ 3 and Józef Langfort 4 1 Department of Health Sciences, Jan Długosz University in Cz˛estochowa,42-200 Cz˛estochowa,Poland; [email protected] (S.L.); [email protected] (A.S.);´ [email protected] (A.P.); [email protected] (W.P.) 2 Urological and Andrological Clinic “Urogen”, 42-600 Tarnowskie Góry, Poland 3 Faculty of Physical Education, Department of Sport, Kazimierz Wielki University in Bydgoszcz, 85-091 Bydgoszcz, Poland; [email protected] 4 Institute of Sport Sciences, The Jerzy Kukuczka Academy of Physical Education, 40-065 Katowice, Poland; [email protected] * Correspondence: [email protected]; Tel.: +48-34-365-5983 or +48-508-204-403 Abstract: The aim of this study was to determine whether, after 8 days of water-only fasting, there are changes in the efficiency of the lower urinary tract, the concentration of sex hormones, and the symptoms of prostate diseases in a group of middle-aged men (n = 14). For this purpose, before and after 8 days of water-only fasting (subjects drank ad libitum moderately mineralized water), and the following somatic and blood concentration measurements were made: total prostate specific antigen (PSA-T), free prostate specific antigen (PSA-F), follicle stimulating hormone (FSH), luteotropic hormone (LH), prolactin (Pr), total testosterone (T-T), free testosterone (T-F), dehydroepiandrosterone (DHEA), sex hormone globulin binding (SHGB), total cholesterol (Ch-T), β-hydroxybutyrate (β-HB).
    [Show full text]
  • Orchiopexy: Surgery for an Undescended Testicle
    In partnership with Primary Children’s Hospital Orchiopexy: Surgery for an undescended testicle Orchiopexy (or-key-oh-PEX-ee) is a procedure that brings the testicle(s) into the scrotum. Why does my child need an orchiopexy? The testicles develop in the abdomen while a baby is in utero and usually move to the scrotum by 6 months old. Your child may need an orchiopexy if their testicle or testicles have not moved to the scrotum by this time. Surgically moving the testicles to the scrotum helps them develop normally and allows healthy growth for future sperm production. What happens during an orchiopexy? During an orchiopexy, your child will be put under general anesthesia. The surgeon will make two small openings in the groin and find the undecended testicle(s). They will make sure the testicle is healthy and then put it into the scrotum. What should I do about nausea When can my child go home after and vomiting? an orchiopexy? After surgery, many children feel nauseated and may Your child can usually go home the same day as the vomit in the first 24 hours. This can be caused by orchiopexy. Watch your child carefully during the medicines during surgery, car movement, or pain ride home to make sure their head and neck don’t medicine after surgery. Help control nausea by: slump forward and close their airway. • Encouraging your child to lie still When can my child eat after • Offering clear liquids, which are important after an orchiopexy? surgery to help prevent dehydration After your child wakes up from surgery, they may • Slowly offering regular foods over a few days until have clear liquids like Pedialyte™, water, apple juice, your child can eat a normal diet sports drinks, ice pops, and plain gelatin.
    [Show full text]
  • Anti-Spermatogenic Effects of Methanolic Extract of Citrullus Colocynthis and Delonix Regia on Male Reproductive Organs of Wistar Rats
    ASJ: International Journal of Health, Safety and Environment (IJHSE) Vol. 6 (09) 30 December, 2020, Pp. 711 – 720 www.academiascholarlyjournal.org/ijhse/index_ijhse.htm ISSN: 2360-9311©Academia Scholarly Journals Also Available@: Archive.org/Payal_et_al. Open access Anti-spermatogenic Effects of Methanolic Extract of Citrullus colocynthis and Delonix regia on Male Reproductive Organs of Wistar Rats Payal Soan1, Ajit Kumar Sharma1 and Ravi Sharma2* 1Department of Botany, St. Wilfred College for Girls, Mansarovar, Jaipur (Rajasthan), India. 2Department of Botany, K.R. College, Mathura; Ex-Founder Principal ESS ESS College of Education Dayalbagh, Agra and Retd. Prof. Botany Agra College, Agra (Dr. B. R. Ambedkar University Agra, Formerly Agra University, Agra) UP India. *Corresponding Authors’ Contact Details: E-mail Address ✉: [email protected]; Phone no ☎: + 91 9897258005 Accepted December 18, 2020 The present investigation was carried out in the laboratory Departments of Botany and Zoology, St. Wilfred College for Girls, Mansarovar and Reproductive Physiology and Endocrinology Section, Centre of Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, Rajasthan, India with fruit extracts of Citrullus colocynthis and Delonix regia on Male Reproductive Organs of Wistar Rats during (2018-2020) at Jaipur, India for evaluation of some andrological parameters such as morphology of spermatozoa, sperm count, motility, fertility index. The experiments were performed with fruit extracts (of C. colocynthis and D. regia) in double distilled water (100 mg/ml) administered orally to Wistar Rats randomly (RBD) divided into three groups with three replicates each: Group_1: Control Distilled water treated Rats; Group_2: Rats treated at 100 mg/kg of C. colocynthis extract 60 days and Group_3: Rats treated at 100 mg/kg of D.
    [Show full text]