COLL 1 Cytosolic internalization of luminescent quantum dots Hedi M. Mattoussi1,
[email protected], Anshika Kapur1, Goutam Palui1, Wentao Wang1, Scott Medina2, Joel Schneider2. (1) Chem Biochem, Florida State University, Tallahassee, Florida, United States (2) Center for Cancer Research, National Cancer Institute, , Frederick, Maryland, United States The remarkable progress made over the past two decades to grow inorganic nanomaterials, combined with careful surface functionalization strategies offer an opportunity to develop novel platforms for use in molecular imaging and as diagnostic tools. A successful integration into biological systems requires devising strategies to promote their intracellular uptake while circumventing endocytosis. We report on the use of an amphiphilic anti-microbial peptide as means of promoting the cytosolic uptake of luminescent QDs. The peptide is synthesized with a terminal cysteine to allow conjugation onto QDs that have been coated with multifunctional metal-coordinating ligands. Using fluorescence imaging and flow cytometry we find that incubating cells with the QD-peptide leads to delivery into the cytoplasm without affecting the cellular morphology or viability. We observed a homogeneous distribution of QD staining throughout the cytoplasm and without co-localization with labelled endosomes. Additional experiments where endocytosis has been eliminated (such as pre-treatment with specific inhibitors) have shown minimal effects on the intracellular QD uptake. COLL 2 Influence of PEGyalation on the interaction of colloids with cells Wolfgang Parak1,2,
[email protected]. (1) Universitaet Marburg, Marburg, Germany (2) CIC Biomagune, San Sebastian, Spain Several homologous nanoparticle libraries were synthesized in which inorganic nanoparticles (Au, FePt) were coated with polyethylene glycol (PEG).