23 (D) Parasite Very Similar to (A), the Micronucleus Is Thickening Prior To

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(d) Parasite very similar to ( a), the micronucleus is thickening prior to division. (e) Two parasites showing the early development of the flagellum ; in one there are two micronucle. (f) A similar parasite, the macronucleus shows evidences of division. Figure 9.—Parasites showing the early protrusion and growth of the flagellum. Stained by Giemsa's stain. Magnification 1,000 diameters. (a) Parasite showing the flagellum protruding as a short thick brush of fine filaments. (b) Twin parasites showing the early protrusion of the flage lum ; in one it can be traced into the vacuole to a point close to the micronucleus. (c) Parasite showing the flagellum passing into the vacuole to a point in close proximity to the micronucleus. (d) Large vacuolated parasite showing the flagellar vacuole passing to the margin prior to rupture. (e) Circular parasite with flagellum seen as a short curved rod ; the macronucleus is dumb-bell shaped and is about to divide. ( f) Large parasite with short flagellum which can be traced up to a chromatic dot close to the micronucleus ; the macronucleus has ust divided. Figure 10.—Parasites showing the method of formation of the true rosette. Stained by Giemsa's stain. Magnification 1 ,000 diameters. (a) Large oval cell consisting of deep blue granular protoplasm containing a number of small circular vacuoles. The original macronucleus has divided and one of the daughter nuclei is about to divide ; associated with the three micronuclei there are three large flagellar vacuoles. (b) Large parasite with two macro-and micronuclei, the former show signs of commencing division ; there are no flagellar vacuoles. (c) A similar cell ; note that the macronuclei are situated at opposite poles. (d) Large vacuolated parasite showing the recent division and commencing separat on of the micronuclei. (e) Elongated parasite, one of the daughter nuclei is commencing to divide. The two micronuclei have already divided. (f) Oval cell showing division of a daughter nucleus ; the micronucleus is thickened and elongated prior to transverse division.