pathogens Article Development of a Multiplex PCR and Magnetic DNA Capture Assay for Detecting Six Species Pathogens of the Genera Anaplasma and Ehrlichia in Canine, Bovine, Caprine and Ovine Blood Samples from Grenada, West Indies Bhumika Sharma 1,* , Roman R. Ganta 2, Diana Stone 1, Andy Alhassan 1 , Marta Lanza-Perea 3, Vanessa Matthew Belmar 1, Inga Karasek 4, Elizabeth Cooksey 4, Catherine M. Butler 4, Kathryn Gibson 1,† and Melinda J. Wilkerson 1 1 Department of Pathobiology, School of Veterinary Medicine, St. George’s University, St. George, West Indies, Grenada;
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[email protected] (A.A.);
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[email protected] (M.J.W.) 2 Center of Excellence of Vector Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA;
[email protected] 3 Department of Small Animal Medicine & Surgery, School of Veterinary Medicine, St Georges University, St. George, West Indies, Grenada;
[email protected] 4 Department of Large Animal Medicine & Surgery, School of Veterinary Medicine, St. George’s University, St. George, West Indies, Grenada;
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[email protected] (E.C.); Citation: Sharma, B.; Ganta, R.R.;
[email protected] (C.M.B.) Stone, D.; Alhassan, A.; Lanza-Perea, * Correspondence:
[email protected] M.; Matthew Belmar, V.; Karasek, I.; † Current address: Office of Field Operations, Food Safety and Inspection Service, Cooksey, E.; Butler, C.M.; Gibson, K.; United States Department of Agriculture, Edinburg, VA 22824, USA. et al. Development of a Multiplex PCR and Magnetic DNA Capture Abstract: Infections with tick-borne pathogens belonging to Anaplasma/Ehrlichia in various vertebrate Assay for Detecting Six Species hosts are a persistent problem resulting in nonspecific clinical signs during early infection.