BIO Web of Conferences 20, 03008 (2020) https://doi.org/10.1051/bioconf/20202003008 ICWEB 2019

The Abundance of Arbuscular Mycorrhiza Infective Propagules Under Galam Stand at Shallow Peat of South

Tri Wira Yuwati1, Annisa Noveani Rusalinda Rahmi2, Safinah Surya Hakim1 and Badruzsaufari2

1Banjarbaru Environment and Forestry Research and Development Institute, Banjarbaru, South Kalimatan 2Faculty of Mathematics and Natural Science, Lambung Mangkurat University, Banjarbaru,

Abstract. Colonization of arbuscular mycorrhiza on plants has been reported to give benefit to plants, especially at extreme sites such as degraded peatland. Galam (Melaleuca cajuputi) is an indigenous peatland species which grows on acidic condition. The number of arbuscular mycorrhiza infective propagules is important to be determined concerning the galam regeneration due to its offered benefits that support colonization. This research aims to determine the abundance of arbuscular mycorrhiza infective propagules under galam stand and to describe symbiotic forms of AMF colonization on the roots of ga lam. The Most Probable Number (MPN) method, wet sieving, and root staining from the modification of Vierherlig et al., 1996, and the calculation of root’s mycorrhizal colonization by grid line technique were the methods that were used in this research. The research used Complete Randomized Design (CRD) with a 5-fold factorial pattern. The results of this study indicated a significant difference between the abundance of AMF under galam stands at the depth of 0-15 cm and 15-30 cm respectively. The results of spores identification showed 4 genera of spores, namely: Glomus, Gigaspora, Scutellospora, and Acaulospora. The structure of root colonizations were hyphae, spores, vesicles, and arbuscular.

1 Introduction 2 Methods Gala m (Melaleuca cajuputi) is a woody plant species grows on shallow to moderately deep peat (1-2 m), 2.1 Soil and root sample collection which can adapt to acidic conditions with the pH range fro m 3-7. This plant has been recorded on shallow to Soil samples and root samples were collected under the moderately deep peat (1-2 m) in South Kalimantan [1]. galam trees of Liang Anggang shallow peat at South Moreover, Galam can grow under flooded conditions Kalimantan. Root sample collection was carried out due to the adaptation of its metabolisms [2]. It is called unproportionally from five galam trees; put in the paperbark in English due to its thin and papery white envelopes and labeled. Soil samples were collected bark [3]. Galam poles are used for construction from 5 galam trees and within each tree, samples were purposes, sawn timber, h igh-quality fuelwood, and replicated from 3 points. The number of soil samples charcoal. Due to the high demand for timber, the was approximately 1 kg for each point at two different availability of Galam has been decreasing due to depth: 0-15 cm and 15-30 cm. The soil samples were overexploitation. Galam is not cultivated. It has relied put in plastic bags and labeled. on natural regeneration and no yield management in the field. Thus, the sustainability of Ga la m trees is 2.2 The Most Probable Number (MPN) method threatened. Most plants, including Galam, associates with To obtain the number of infective propagules, a series arbuscular mycorrhiza fungi (AMF). AMF has the main of 4-fo ld d ilutions were prepared. The soil samples of 1 three functions including increasing plant growth, kg were air-dried and sieved at 0.5 c m x 0.5 c m sieve. enhancing the absorption of soil nutrients, and This sample was used as a base for the next dilution and protecting plants from the pest and disease attack and labeled substrate A. Then, 4 kg of soil sample was critica l site condition [4]. To conserve Galam, we need autoclave-sterilized, sieved, and dried. This sample is to know the benefit of AMF colonization on Galam. labeled as substrate B. Furthermore, 11 kg of soil was One of the ways to determine the benefit of AMF autoclave-sterilized, un-sieved. This sample then colonization is by providing the number of infective labeled as substrate C. The MPN method was then propagules under the Galam trees. Infective propagules started with putting 100 g of substrate C in each pot. including spores and sporocarps, extraradical mycelium, Then, the dilution of substrate A and B was applied 50 and colonized roots; establish associations when they g in each pot. The top layer of the pots was put 50 g of contact potential host-roots [5]. The objective of this substrate C. Three maize seeds were then sown in each research is to determine the abundance of arbuscular pot, watered daily, and grown for 4 weeks. After 4 mycorrhiza infective propagules under Galam stand and weeks, the plant’s roots were then harvested, cleaned, to describe symbiotic forms of AMF colonization on the and stained with the modification of the Vie rheilig roots of Galam. method [6]. In this method, the root samples were

© The Authors, published by EDP Sciences. This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/). BIO Web of Conferences 20, 03008 (2020) https://doi.org/10.1051/bioconf/20202003008 ICWEB 2019

washed with tap water, soaked them in KOH 10% for arbuscular mycorrhiza infective propagules under the 24 hours, soaked in HCl 0.1 N for 24 hours, and ink + Galam stand was ± 75298,29. vinegar 5% solution for 24 hours. The stained root samples were then checked for mycorrhizal 3.2 The arbuscular mycorrhiza spores colonization, counted, and reported as infected (+) or not infected (-). The arbuscular mycorrhiza spores that were isolated under the Galam trees were varied. There were six 2.3 The wet sieving method for isolating the species namely Glo mus sp. 1, Glomus sp. 2, Glomus AMF spores sp.3, Gigaspora sp., Acaulospora sp., and Scutellospora sp. The spores were presented in Figure 1. One hundred grams of soil sample was mixed with 1000 ml of tap water and mixed well. Then, it was poured onto a series of sieve 250, 125, 75, and 63 µm. The particle that was trapped in the sieve was then moved onto a Petri dish, checked, and counted under a stereomicroscope. The spores were then distinguished based on color and size.

2.4 Morphological identification of arbuscular mycorrhiza spores

There were six genera of arbuscular mycorrhiza spores which form a symbiosis with plants namely Glomus, Gigaspora, Scutellospora, Acaulospora, Enthrophospora, and Sclerocystis. The spores were identified based on color, wall layer, Melzer’s reaction, bulbous suspensor, and sustending hyphae [7]. Figure 1. Arbuscular mycorrhiza spores under Galam stand at shallow peat of South Kalimantan 2.5 Data analysis

The infective propagules were counted using a formula The average number of spores per 100-gram soil is Log Ω = x . log a – K presented in Table 2. Ω: the number of infective propagules Table 2 . The average number of spores per 100-gram X: average number of infected pots soil under galam trees. a: dilution factors Mean number of spores per Soil Depth K: taken from Sieverding [8]. 100-gram soil The number of spores and the arbuscular mycorrhiza 0-15 cm 508.4 colonization in plant’s roots was presented 15-30 cm 337.2 descriptively.

The average number of spores under galam trees at the soil depth of 0-15 cm and 15-30cm were 508.4 and 3 Results 337.2, respectively.

3.1 Number of infectives propagules 3.3 Colonization of arbuscular mycorrhiza on roots The number of infective propagules under the Galam stand is presented in Table 1. The percentage of roots colonization is presented in Table 3. Table 1 . The number of infective propagules under the Galam stand. Table 3 . Arbuscular mycorrhiza colonization on galam Number of infective propagules (per roots at shallow peat of South Kalimantan. Sample number 50gram soil) Soil depth Colonization O’Connor 1 ± 85113.80 (cm) percentage (%) (2001) 2 ± 55667.28 0-15 70 High 3 ± 85113.80 15-30 57.42 High

The number of infective propagules under the The arbuscular mycorrhiza colonization on the Galam stand varied with the lowest of ± 55667,28 and roots of galam at shallow peat of Kalimantan at 0-15 c m the highest of ± 85113,8. The average number of and 15-30 cm depth were 70 % and 57.42%,

2 BIO Web of Conferences 20, 03008 (2020) https://doi.org/10.1051/bioconf/20202003008 ICWEB 2019 washed with tap water, soaked them in KOH 10% for arbuscular mycorrhiza infective propagules under the respectively. Both were categorized as high according moisture is higher. Moisture could accelerate the spores 24 hours, soaked in HCl 0.1 N for 24 hours, and ink + Galam stand was ± 75298,29. to O’Connor [10]. to germinate [15]. It means, the deeper the soil, the vinegar 5% solution for 24 hours. The stained root The symbiotic forms of AMF colonization on the arbuscular mycorrhiza could be in the form of hyphae samples were then checked for mycorrhizal 3.2 The arbuscular mycorrhiza spores Galam roots were found namely arbuscular and than spore. Moreover, Delv ian [15] stated that high soil colonization, counted, and reported as infected (+) or vesicular (Figure 2). moisture content could accelerate the spore to not infected (-). The arbuscular mycorrhiza spores that were isolated germinate and form colonization on the host plant’s under the Galam trees were varied. There were six roots. On the contrary, the formulation of new spores 2.3 The wet sieving method for isolating the species namely Glo mus sp. 1, Glomus sp. 2, Glomus will increase the dry condition and roots colonization AMF spores sp.3, Gigaspora sp., Acaulospora sp., and Scutellospora will be lower. It is a natural response of arbuscular sp. The spores were presented in Figure 1. mycorrhiza to keep its existence in nature. One hundred grams of soil sample was mixed with One of the ways to enhance land productivity is 1000 ml of tap water and mixed well. Then, it was by silvicultural techniques; one of them is the utilization poured onto a series of sieve 250, 125, 75, and 63 µm. of biofert ilizer. Considering the number of infective The particle that was trapped in the sieve was then propagules, s ome spores, and colonization rates, the moved onto a Petri dish, checked, and counted under a arbuscular mycorrhiza spores under the galam trees at stereomicroscope. The spores were then distinguished shallow peat of South Kalimantan is promising to be Figure 2. The symbiotic forms of AMF colonization on based on color and size. utilized as biofertilizer. Galam roots showing arbuscular (left) and vesicular (right)  2.4 Morphological identification of arbuscular  mycorrhiza spores The AMF arbuscular structures look like a tree; 5 Conclusion consists of intraradical hyphae. Arbuscules functions as There were six genera of arbuscular mycorrhiza spores a place for the exchange of nutrients and carbon The average number of arbuscular mycorrhiza infective which form a symbiosis with plants namely Glomus, between AMF and host plant. The molecular and propagules under the Galam stand of shallow peat Gigaspora, Scutellospora, Acaulospora, structural organizations of arbuscular facilitate and South Kalimantan were 55667-85113 propagules p er Enthrophospora, and Sclerocystis. The spores were regulate the processes of nutrient exchange [9]. The 50-gra m soil. The number of spores at 0-15 cm and identified based on color, wall layer, Melzer’s reaction, vesicles are terminal swellings of hyphae formed inter 15-30c m soil depth were 508 and 337, respectively. Figure 1. Arbuscular mycorrhiza spores under Galam bulbous suspensor, and sustending hyphae [7]. and intracellularly having a storage function [7]. There were four genera of arbuscular mycorrhiza spores stand at shallow peat of South Kalimantan found namely Glomus, Gigaspora, Acaulospora, and 2.5 Data analysis Scutellospora. Among the four genera, Glomus was

dominantly found. The colonization of arbuscular The average number of spores per 100-gram soil is The infective propagules were counted using a formula 4 Discussion mycorrhiza on the roots was ranging from 57 – 70%; presented in Table 2. Log Ω = x . log a – K categorized as high. These findings showed that Galam Ω: the number of infective propagules Table 2 . The average number of spores per 100-gram The average number of infective propagules under the was able to get the benefit of AMF colonization and the X: average number of infected pots soil under galam trees. galam tree at shallow peat of South Kalimantan was ± soil under galam trees was the potential to be developed a: dilution factors Mean number of spores per 75298,29 per 50-gram soil; or 1505 per gram soil. as biofertilizer. Soil Depth K: taken from Sieverding [8]. 100-gram soil According to The Indonesian Ministry of Agriculture The number of spores and the arbuscular mycorrhiza 0-15 cm 508.4 Decree No. 70/ 2011 on Biofertilizer Standard, the colonization in plant’s roots was presented 15-30 cm 337.2 number of arbuscular mycorrhiza propagules shall descriptively. higher than 50 per gram soil. Moreover, if we are References

looking at the root colonization at 0-15 cm and 15-30 The average number of spores under galam trees at the cm, both exceeded more than 50%. According to soil depth of 0-15 cm and 15-30cm were 508.4 and 1. W. Giesen. Vegetation of the Negara River Basin. O’Connor [10], it was categorized as high colonization. 337.2, respectively. In Zieren, M., Permana, T and Giesen, W (eds.). 3 Results Thus, according to The Indonesian Ministry of Proceeding of Workshop on Conservation of Agriculture Decree No. 70/ 2011 on Biofertilizer Sungai Negara Wetlands Barito Basin, Banjarbaru 3.3 Colonization of arbuscular mycorrhiza on 3.1 Number of infectives propagules Standard, the minimum percentage of colonization for (6-8 March 1989), (1990) roots the biofertilizer is more than 50%. It means, from the 2. S. Kogawara, T. Yamanoshita, M. Norisada, M. The number of infective propagules under the Galam number of infective propagules and arbuscular The percentage of roots colonization is presented in Masumori, and K. Ko jima. Photosynthesis and stand is presented in Table 1. mycorrhiza colonization, we can determine that Galam Table 3. photoassimilate transport during root hypoxia in Table 1 . The number of infective propagules under the can get the benefit from arbuscular mycorrhiza. Melaleuca cajuputi, a flood-tolerant species, and in Galam stand. Table 3 . Arbuscular mycorrhiza colonization on galam Glomus was the species that most found under the Eucalyptus camaldulensis, a moderately Number of infective propagules (per roots at shallow peat of South Kalimantan. Galam trees at shallow peat of South Kalimantan, flood-tolerant species. Tree Physiology 26, 1413– Sample number 50gram soil) Soil depth Colonization O’Connor Glomus is known to be adaptable in various soil and has 1423, (2006) 1 ± 85113.80 (cm) percentage (%) (2001) the widest habitat [11]. Glomus has a high tolerance to 3. W. Giesen. Case Study: Melaleuca cajuputi 2 ± 55667.28 0-15 70 High extreme conditions and can form symbiosis [12]. (gela m) – a useful species and an option for 3 ± 85113.80 15-30 57.42 High Ramadhani [13] added that Gigaspora is also able to paludiculture in degraded peatlands. Sustainable survive in the extreme condition such as acidic or Peatlands for People & Climate (SPPC) Project. drought. Yu wat i et al [14] also found that Glomus sp The number of infective propagules under the The arbuscular mycorrhiza colonization on the Wetlands International. p 16, (2015) was dominantly found at deep after burnt peatland at rd Galam stand varied with the lowest of ± 55667,28 and roots of galam at shallow peat of Kalimantan at 0-15 c m 4. S.E. Smith, D.J. Read. Mycorrhizal Symbiosis. 3 the highest of ± 85113,8. The average number of and 15-30 cm depth were 70 % and 57.42%, . The number of spores was higher eds. Elsevier. Amsterdam, (2008) at 0-15 cm than 15-30 cm. The deeper the peat soil, the

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