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Impact of Non-Proteinogenic Amino Acids in the Discovery And

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Impact of Non-Proteinogenic Amino Acids in the Discovery And Amino Acids (2020) 52:1207–1226 https://doi.org/10.1007/s00726-020-02890-9 REVIEW ARTICLE Impact of non‑proteinogenic amino acids in the discovery and development of peptide therapeutics Yun Ding1 · Joey Paolo Ting1 · Jinsha Liu1 · Shams Al‑Azzam2 · Priyanka Pandya1 · Sepideh Afshar1 Received: 24 June 2020 / Accepted: 5 September 2020 / Published online: 18 September 2020 © The Author(s) 2020 Abstract With the development of modern chemistry and biology, non-proteinogenic amino acids (NPAAs) have become a powerful tool for developing peptide-based drug candidates. Drug-like properties of peptidic medicines, due to the smaller size and simpler structure compared to large proteins, can be changed fundamentally by introducing NPAAs in its sequence. While peptides composed of natural amino acids can be used as drug candidates, the majority have shown to be less stable in biological conditions. The impact of NPAA incorporation can be extremely benefcial in improving the stability, potency, permeability, and bioavailability of peptide-based therapies. Conversely, undesired efects such as toxicity or immunogenic- ity should also be considered. The impact of NPAAs in the development of peptide-based therapeutics is reviewed in this article. Further, numerous examples of peptides containing NPAAs are presented to highlight the ongoing development in peptide-based therapeutics. Keywords Non-proteinogenic amino acid · Peptide therapeutic · Discovery · Development Abbreviations H Histidine A Alanine HIV-1 Human immunodefciency virus-1 AA Amino acid HLA Human leukocyte antigen Aβ Amyloid-β I Isoleucine ACE Angiotensin-converting enzyme K Lysine AD Alzheimer’s disease M Methionine BBB Blood–brain barrier N Asparagine C Cysteine NAA Natural amino acid Cha Cyclohexylalanine NRP Non-ribosomal peptide CNS Central nervous system Orn Ornithine D Aspartic acid P Proline DPP-3 Dipeptidyl peptidase 3 PAMPA Parallel artifcial membrane permeability assay DPP-4 Dipeptidyl peptidase 4 PEG Polyethylene glycol chain E Glutamic acid PTM Post-translation modifcation F Phenylalanine Q Glutamine G Glycine R Arginine GLP-1 Glucagon-like peptide S Serine T Threonine NPAA Non-proteinogenic amino acid Handling Editor: J. D. Wade. V Valine * Sepideh Afshar W Tryptophan [email protected] Y Tyrosine 1 Protein Engineering, Lilly Biotechnology Center, Eli Lilly and Company, San Diego, CA 92121, USA 2 Professional Scientifc Services, Eurofns Lancaster Laboratories, Lancaster, PA 17605, USA Vol.:(0123456789)1 3 1208 Y. Ding et al. Introduction drug space. Many natural and synthetically optimized NRPs have made it to the clinic, including penicillin precursor Peptides have drawn much attention in the drug discovery ACV-tripeptide, immunosuppressant drug cyclosporine, space. Since the frst insulin native peptide was isolated and antibiotic vancomycin and many others (Saito et al. 1994; used for treating diabetes in the 1920s, over 150 peptide Ofenzeller et al. 1996; Byford et al. 1997; Mootz and Mara- therapies have entered clinical studies and over 60 have been hiel 1997; van Wageningen et al. 1998; Keating et al. 2001). approved (Lau and Dunn 2018). Peptides are attractive drug Here, we summarize diferent types of naturally occurring candidates because of their high selectivity, low toxicity, and and synthetically made non-proteinogenic amino acids. relative ease of synthesis. The structural diversity of the pep- Natural amino acids, except for Gly, exists in two enan- tides is driven by proteinogenic and non-proteinogenic build- tiomer confgurations: L and D. The L enantiomers of amino ing blocks. Non-proteinogenic amino acids (NPAAs) are not acids are the dominant form in nature. In fact, some organ- naturally encoded in the human genetic code or found in the isms have completely excluded D-amino acids to support polypeptide chains. On the other hand, in organisms such as peptide homochirality. On the other hand, many prokaryotic bacteria, fungi, plants, and marines, NPAAs are essential and eukaryotic organisms utilize D-amino acids in free form building blocks of polypeptide chains. Numerous NPAAs or in the context of polypeptide chains to induce a certain found in nature are analogs of natural amino acids (NAAs, or biological function. In Gram-positive eubacteria, D-Ala and proteinogenic amino acids) (Fichtner et al. 2017) and some D-Gln are produced in high quantities in the peptidoglycan exist as secondary metabolites in many organisms. layer of the cell wall to provide resistance from proteolysis While bioactive native peptides can intrinsically be used (Hancock 1960). Toxic peptides, such as conotoxins isolated as drug candidates, their low bioavailability and short circu- from carnivorous marine gastropod mollusks venom, con- lating plasma half-life hinder their direct use as therapeutics tain D-Trp or D-Leu to block neuromuscular transmission in and often require structural optimization. NPAAs provide a mammals (Jimenez et al. 2001). The toxic peptide, agatox- toolbox of physiochemical properties that expand from NAAs ins, isolated form North American funnel-web spider Age- (Stevenazzi et al. 2014; Xue et al. 2018). Various types of lenopsis aperta showed compromised potency as a venom chemical and enzymatic synthesis methodologies have made when D-Ser was substituted with Ser (Kuwada et al. 1994; NPAAs widely available (Stevenazzi et al. 2014a; Xue et al. Jimenez et al. 2001). D-Phe in gramicidin S and polymyxin B 2018). Moreover, chemical and biosynthetic strategies such contributes to peptides’ antimicrobial efcacy in membrane as orthogonal synthetase-tRNA approach and in vitro transla- disruption (Falagas et al. 2006). In humans and rodents, free tion methods by mRNA display have enabled the construction D-Ser and D-Asp are distributed at high concentrations in dif- of diverse peptide libraries that comprise NAA and NPAAs. ferent parts of the brain throughout embryotic development These libraries have efectively been employed for target and postnatal life. D-Ser and D-Asp selectively potentiates screening and selection to identify specifc peptides (Kent N-methyl-D-aspartate (NMDA)-type excitatory amino acid 1988; Tian et al. 2004; Ma and Hartman 2012). A few exam- receptor at its Glycine site to mediate neurotransmission ples of this approach will be highlighted here. (Hashimoto and Oka 1997). D-Asp is also found in elderly This review will focus on the roles of NPAAs in modulating people in various tissues such as tooth, bone, brain, and eye stability, potency, permeability, oral bioavailability, and immu- lenses (Fujii 2002). D-Ser has been detected in β amyloid nogenicity in peptides. We will present the predicament faced proteins of Alzheimer’s patients (Kaneko et al. 1995). in the oral peptide therapeutic space and how NPAA incor- Post-translation modification (PTM) of proteins and poration can play a role in improving peptide–drug pharma- peptides improves their functional and biological diversity cokinetic properties. While there is no magic combination of beyond the 20 natural amino acids. There are over 20 difer- NPAA and NAA that can address challenges associated with ent types of modifcations to the natural amino acids. The peptide drug discovery, this review will provide numerous PTMs such as phosphorylation, acetylation, and disulfde examples that validate the use of NPAA as a powerful tool to formation can be reversible and are commonly associated design stable, active, selective, and potent peptide therapeutics. with signaling and metabolic processes. Irreversible PTM reactions are typically associated with physiological cascade processes such as blood coagulation, peptide bond cleavage, Naturally occurring and synthetic NPAAs and protein splicing. Methylation is the most common PTM of Lys; however, some Lys residues in transcription factors Bacteria and fungi utilize non-ribosomal peptide synthetases are modifed by ubiquitination, sumoylation, and acetyla- (NRPSs) to synthesize hundreds of non-proteinogenic amino tion to regulate target gene expression in response to extra- acids for incorporation in non-ribosomal peptides (NRP). cellular signals. On a further note, modifcation of a Lys These bioactive peptides have revolutionized the peptide residue can infuence the modifcation of the neighboring residues. Sumoylation of Lys in human and yeast may have 1 3 Impact of non-proteinogenic amino acids in the discovery and development of peptide… 1209 a protective functionality against proteolysis (Freiman and increase metabolic stability, and lower clearance rate, Tjian 2003). For example, sumoylation of the Lys21 in IκBα thereby improving bioavailability (Sola and Griebenow prevents its ubiquitination and increases protein resistance 2009; Moradi et al. 2016). A pioneering example is gly- to proteasome-mediated degradation (Desterro et al. 1998). cosylated analogs of the oral peptide therapy somatostatin. Phosphorylation in prokaryotic and eukaryotic organisms The glycosylated version retained its original activity and is catalyzed by various kinases, where a phosphate group is showed ten times higher bioavailability (Albert et al. 1993). transferred into the hydroxyl-containing amino acid. Phos- Peptide half-life is expanded through lipidation, where long- phorylation is a common modifcation of Ser, Thr, and Tyr to chain saturated lipid is acylated to an amino acid to facilitate mediate receptor activation and cellular transduction signal- its binding to a carrier serum protein as demonstrated in the ing. Another essential modifcation
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