DOCSLIB.ORG

Comprehensive Transcriptome Profiling of Peripheral Blood

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Comprehensive Transcriptome Profiling of Peripheral Blood Int. J. Med. Sci. 2020, Vol. 17 2077 Ivyspring International Publisher International Journal of Medical Sciences 2020; 17(14): 2077-2086. doi: 10.7150/ijms.46910 Research Paper Comprehensive Transcriptome Profiling of Peripheral Blood Mononuclear Cells from Patients with Sepsis Tianzhou Wu1*, Xi Liang1*, Yongpo Jiang2*, Qi Chen1, Huaping Zhang6, Sheng Zhang2, Chao Zhang3, Yuhang Lv6, Jiaojiao Xin1,4, Jing Jiang1,4, Dongyan Shi1,4, Xin Chen1,5, Jun Li1,4 and Yinghe Xu6 1. Precision Medicine Center, Taizhou Central Hospital, Taizhou University Medical School, Taizhou, China. 2. Department of intensive care unit, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Taizhou, China. 3. Department of intensive care unit, Taizhou Enze Medical Center (Group) Enze Hospital, Taizhou, China. 4. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine. 5. Institute of Pharmaceutical Biotechnology, Zhejiang University School of Medicine, Hangzhou, China. 6. Department of intensive care unit, Taizhou Central Hospital, Taizhou University Medical School, Taizhou, China. *These authors contributed equally to this work. Corresponding authors: Yinghe Xu. M.D. Professor, Department of intensive care unit, Taizhou Central Hospital, Taizhou University Medical School, Taizhou, China, E-mail: [email protected]; Co-corresponding: Jun Li. M.D. & Ph.D. Professor, E-mail: [email protected]; Xin Chen. Ph.D. Professor. E-mail: [email protected]. © The author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. Received: 2020.04.11; Accepted: 2020.07.14; Published: 2020.07.25 Abstract Background: Sepsis, as a clinical emergency, usually causes multiorgan dysfunction and can lead to high mortality. Establishment of specific and sensitive biomarkers for early diagnosis is critical to identify patients who would benefit from targeted therapy. In this study, we investigated this syndrome by analyzing the transcriptome of peripheral blood mononuclear cells (PBMCs) from patients with sepsis and identified sepsis-specific biomarkers. Methods: In this study, a total of 87 patients with sepsis and 40 healthy controls from a prospective multicenter cohort were enrolled. Samples from 44 subjects (24 patients with sepsis and 20 healthy controls) were sequenced and the remaining patients were included in the validation group. Using high-throughput sequencing, a gene expression profile of PBMCs from patients with sepsis was generated to elucidate the pathophysiology of sepsis and identify sepsis-specific biomarkers. Results: Principal component analysis (PCA) and unsupervised hierarchical cluster analysis showed that patients with sepsis separated from healthy controls. A total of 1639 differentially expressed genes (DEGs) were identified (|log2 fold change|>2, adjusted P value <0.05) between these two groups, with 1278 (78.0%) upregulated and 361 (22.0%) downregulated in patients with sepsis. Gene Ontology (GO) analysis of the upregulated DEGs identified 194 GO terms that were clustered into 27 groups, and analysis of the downregulated DEGs identified 20 GO terms that were clustered into 4 groups. Four unique genes were identified that could be predictive of patients with sepsis. External validation of the four genes using quantitative real-time polymerase chain reaction (qRT-PCR) was consistent with the results of mRNA sequencing, revealing their potential in sepsis diagnosis. Conclusions: The transcriptome characteristics of PBMCs, which were significantly altered in sepsis patients, provide new insights into sepsis pathogenesis. The four identified gene expression changes differentiated patients with sepsis from healthy subjects, which could serve as a convenient tool contributing to sepsis diagnosis. Key words: sepsis; transcriptomics; peripheral blood mononuclear cells; biomarker Introduction Sepsis is a potentially life-threatening condition damage, organ failure, and death. The incidence of in the intensive care unit (ICU) caused by the body's sepsis is very high (2.8 million deaths per year response to an infection, that can lead to tissue worldwide) in adults, with high mortality rates of 10– http://www.medsci.org Int. J. Med. Sci. 2020, Vol. 17 2078 50%, depending on age and disease severity [1, 2]. Study design Despite the burden on patients and the healthcare Patients were prospectively screened at 3 service system, treatment remains mainly futile. Thus, participating ICUs and enrolled between April 1, precise diagnosis and targeted therapy for sepsis is 2019, and November 20, 2019. The last follow-up was crucial to reducing mortality, with every hour of completed on February 18, 2020. Adult patients (aged delay increasing mortality risk [3]. >18 years) who were admitted to the ICU with sepsis Recent findings in transcriptome analysis have were enrolled in the study. Healthy subjects (with 0 in given us a greater understanding of the mechanisms SOFA and without infection) were also recruited as behind diseases. Genome-wide gene expression the control group from Physical Examination Center profiling is extensively used to discover new potential during the same time. Sepsis was defined as the biomarkers for the diagnosis or prediction of disease presence of an infection combined with an acute severity and identification of novel drug targets. change in SOFA score of 2 or more points [10]. However, tissue sampling has limited the accurate Detailed clinical data and outcomes for all enrolled investigation of sepsis pathogenesis. Therefore, blood patients were collected and recorded in case report can be used as a surrogate and can be obtained with a forms at admission and during the 28/90-day minimally invasive procedure. Many studies have follow-up. indicated that the messenger ribonucleic acid (mRNA) expression levels of specific genes in peripheral blood Methods of mRNA sequencing/mRNA mononuclear cells (PBMCs) can serve as a signature of sequencing sample preparation specific diseases, such as active pulmonary Samples were obtained from 105 patients with tuberculosis [4] and enterocolitis syndrome [5]. an acute change in SOFA score of 2 or more points Recently, many genes have also been identified for following admission to ICU, and 10 mL whole blood sepsis diagnosis and prognosis; for example, sample were taken. PBMCs were immediately SeptiCyte Lab combined with CEACAM4, LAMP1, isolated by Ficoll-PaqueTM PLUS medium (GE PLA2G7, and PLAC8 genes was identified to Healthcare, Uppsala, Sweden). Eighty-seven patients determine which patients had sepsis [6]. Meanwhile, diagnosed with infection were finally defined as seven genes (DYRK2, CCNB1IP1, TDRD9, ZAP70, sepsis, and their samples were used for mRNA ARL14EP, MDC1, and ADGRE3) had been identified sequencing or validation, as well as, samples of 40 as predictors of septic patients and their relationships healthy controls. Total RNA, which was freshly with clinical outcomes were observed [7]. However, extracted, was stored at -80°C for subsequent testing. these biomarkers lack sensitivity and specificity and A sequencing library was then prepared following the have been analyzed by microarray data [8, 9]. manufacturer’s instructions (TruSeqTM Small RNA We hypothesized that the pathological process of Sample Preparation Kit, Illumina, San Diego, CA, sepsis changes the transcriptome profile of PBMCs. In USA), consisting of purification of poly-A-containing this study, we characterized the transcriptome profile mRNA molecules, fragmentation of mRNA, end of PBMCs in patients with sepsis using high- repair, addition of a single ‘A’ base, ligation of throughput sequencing to establish alterations adapters, reverse transcription, PCR amplification present in gene expression patterns and identify and pooled gel purification steps. The pooled library potential biomarkers that could serve as a convenient consisted of sequences with lengths of approximately tool to improve sepsis diagnosis. 250 nucleotides. The library was sequenced using the HiSeq 2500 sequencing system (Illumina, San Diego, Methods CA, USA). The study was approved by the Clinical Research Ethics Committee of Taizhou Central Quantitative real-time polymerase chain Hospital (Taizhou University Medical School) reaction (Registration number: 2019-016, Principal Quantitative real-time polymerase chain reaction investigator: Yinghe Xu, Date of registration: (qRT-PCR) was used to validate the results of the February 26, 2019). This study was registered in the transcriptome analysis. Total RNA was isolated from Chinese Clinical Trial Registry (ChiCTR1900022081, PBMCs and reverse transcribed. qRT-PCR was Principal investigator: Yinghe Xu, Date of registration: performed using a two-step protocol. Complementary March 21, 2019). The trial was registered prior to DNA (cDNA) was synthesized using a RevertAid patient enrollment. Written informed consent was First Strand cDNA Synthesis Kit (Thermo, IL, USA). obtained from all participators or their legal The amount of cDNA was optimized so that representative. amplification of both control gene cDNA and the cDNAs of interest were in the exponential phase. http://www.medsci.org Int. J. Med. Sci. 2020, Vol. 17 2079 qRT-PCRs were performed with PowerUp SYBR enrichment analysis.
Load more

Recommended publications
  • The Neuroprotective Effect of Tea Polyphenols on the Regulation of Intestinal Flora
    molecules Review The Neuroprotective Effect of Tea Polyphenols on the Regulation of Intestinal Flora Zhicheng Zhang 1,2,3, Yuting Zhang 4, Junmin Li 2,3,*, Chengxin Fu 1,* and Xin Zhang 4,* 1 Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, China; [email protected] 2 Taizhou Biomedical Industry Research Institute Co., Ltd., Taizhou 317000, China 3 College of Life Sciences, Taizhou University, Taizhou 317000, China 4 Department of Food Science and Engineering, Ningbo University, Ningbo 315211, China; [email protected] * Correspondence: [email protected] (J.L.); [email protected] (C.F.); [email protected] (X.Z.) Abstract: Tea polyphenols (TPs) are the general compounds of natural polyhydroxyphenols extracted in tea. Although a large number of studies have shown that TPs have obvious neuroprotective and neuro repair effects, they are limited due to the low bioavailability in vivo. However, TPs can act indirectly on the central nervous system by affecting the “microflora–gut–brain axis”, in which the microbiota and its composition represent a factor that determines brain health. Bidirectional communication between the intestinal microflora and the brain (microbe–gut–brain axis) occurs through a variety of pathways, including the vagus nerve, immune system, neuroendocrine pathways, and bacteria-derived metabolites. This axis has been shown to influence neurotransmission and behavior, which is usually associated with neuropsychiatric disorders. In this review, we discuss that TPs and their metabolites may provide benefits by restoring the imbalance of intestinal microbiota and Citation: Zhang, Z.; Zhang, Y.; Li, J.; that TPs are metabolized by intestinal flora, to provide a new idea for TPs to play a neuroprotective Fu, C.; Zhang, X.
    [Show full text]
  • Ecological Engineering Effects of Plant Diversity on Microbial Biomass And
    Ecological Engineering 36 (2010) 62–68 Contents lists available at ScienceDirect Ecological Engineering journal homepage: www.elsevier.com/locate/ecoleng Effects of plant diversity on microbial biomass and community metabolic profiles in a full-scale constructed wetland Chong-Bang Zhang a, Jiang Wang a, Wen-Li Liu a, Si-Xi Zhu b, Han-Liang Ge b, Scott X. Chang c, Jie Chang b, Ying Ge b,∗ a School of Life Sciences, Taizhou University, Linhai 317000, PR China b College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China c Department of Renewable Resources, University of Alberta, Edmonton, Alberta, Canada T6G 2E3 article info abstract Article history: There has been less understanding of relations of microbial community patterns with plant diversity Received 14 June 2009 in constructed wetlands. We conducted a single full-scale subsurface vertical flow constructed wetland Received in revised form 1 August 2009 (SVFCW, 1000 m2) study focusing on domestic wastewater processing. This study measured the size and Accepted 14 September 2009 structure of microbial community using fumigation extraction and BIOLOG EcoplateTM techniques, to examine the effects of macrophyte diversity on microbial communities that are critical in treatment effi- ciency of constructed wetlands. We also determined the relationship of plant diversity (species richness) Keywords: with its biomass production under disturbance of the same wastewater supply. Linear regression anal- Subsurface vertical flow constructed wetland ysis showed that plant biomass production strongly correlated with plant species richness (R = 0.407, Plant diversity P < 0.001). Increase in plant species richness increased microbial biomass carbon and nitrogen (R = 0.494, Microbial community P < 0.001; R = 0.465, P < 0.001) and utilization of amino acids on Ecoplates (R = 0.235, P = 0.03), but lim- CLPP ited the utilization of amine/amides (R = −0.338, P = 0.013).
    [Show full text]
  • A Complete Collection of Chinese Institutes and Universities For
    Study in China——All China Universities All China Universities 2019.12 Please download WeChat app and follow our official account (scan QR code below or add WeChat ID: A15810086985), to start your application journey. Study in China——All China Universities Anhui 安徽 【www.studyinanhui.com】 1. Anhui University 安徽大学 http://ahu.admissions.cn 2. University of Science and Technology of China 中国科学技术大学 http://ustc.admissions.cn 3. Hefei University of Technology 合肥工业大学 http://hfut.admissions.cn 4. Anhui University of Technology 安徽工业大学 http://ahut.admissions.cn 5. Anhui University of Science and Technology 安徽理工大学 http://aust.admissions.cn 6. Anhui Engineering University 安徽工程大学 http://ahpu.admissions.cn 7. Anhui Agricultural University 安徽农业大学 http://ahau.admissions.cn 8. Anhui Medical University 安徽医科大学 http://ahmu.admissions.cn 9. Bengbu Medical College 蚌埠医学院 http://bbmc.admissions.cn 10. Wannan Medical College 皖南医学院 http://wnmc.admissions.cn 11. Anhui University of Chinese Medicine 安徽中医药大学 http://ahtcm.admissions.cn 12. Anhui Normal University 安徽师范大学 http://ahnu.admissions.cn 13. Fuyang Normal University 阜阳师范大学 http://fynu.admissions.cn 14. Anqing Teachers College 安庆师范大学 http://aqtc.admissions.cn 15. Huaibei Normal University 淮北师范大学 http://chnu.admissions.cn Please download WeChat app and follow our official account (scan QR code below or add WeChat ID: A15810086985), to start your application journey. Study in China——All China Universities 16. Huangshan University 黄山学院 http://hsu.admissions.cn 17. Western Anhui University 皖西学院 http://wxc.admissions.cn 18. Chuzhou University 滁州学院 http://chzu.admissions.cn 19. Anhui University of Finance & Economics 安徽财经大学 http://aufe.admissions.cn 20. Suzhou University 宿州学院 http://ahszu.admissions.cn 21.
    [Show full text]
  • University of Leeds Chinese Accepted Institution List 2021
    University of Leeds Chinese accepted Institution List 2021 This list applies to courses in: All Engineering and Computing courses School of Mathematics School of Education School of Politics and International Studies School of Sociology and Social Policy GPA Requirements 2:1 = 75-85% 2:2 = 70-80% Please visit https://courses.leeds.ac.uk to find out which courses require a 2:1 and a 2:2. Please note: This document is to be used as a guide only. Final decisions will be made by the University of Leeds admissions teams.
    [Show full text]
  • A Study on Textless Back Translation of China Boy from Acculturation Perspective
    ISSN 1923-1555[Print] Studies in Literature and Language ISSN 1923-1563[Online] Vol. 16, No. 2, 2018, pp. 10-18 www.cscanada.net DOI:10.3968/10330 www.cscanada.org A Study on Textless Back Translation of China Boy From Acculturation Perspective CHEN Lihong[a]; LI Changbao[b],* [a]MA, Lecturer, School of Foreign Languages, Taizhou University, reproduction of host cultural elements; the translation, Linhai, China. guided by marginalization strategy usually gives rise [b]Ph.D., Professor, School of Foreign Languages, Zhejiang University of Finance & Economics, Hangzhou, China. to mistranslations, in which translators usually need to *Corresponding author. refer to domestication as so to correctly convey their connotations. Supported by National Social Sciences Foundation Project Acculturation; China Boy; Textless back (16BYY011); Overseas Training Project for University Teachers from Key words: the Department of Education of Zhejiang Province “A Study on Textless translation; Language features; Translation strategies Back Translation of China Boy From Acculturation Perspective” (JW2017002). Chen, L. H., & Li, C. B. (2018). A Study on Textless Back Translation of China Boy From Acculturation Perspective. Received 14 January 2018; accepted 12 March 2018 Studies in Literature and Language, 16(2), 10-18. Available from: Published online 26 April 2018 http://www.cscanada.net/index.php/sll/article/view/10330 DOI: http://dx.doi.org/10.3968/10330 Abstract Textless back translation refers to the kind of back translation in which the translator retranslates China- themed works written in English language back into the INTRODUCTION Chinese language. Based on English-Chinese bilingual Back translation refers to “re-translate the translation corpus and acculturation theory, this paper attempts to text back into the source language” (Fang, 2011, p.97).
    [Show full text]
  • 1 Please Read These Instructions Carefully
    PLEASE READ THESE INSTRUCTIONS CAREFULLY. MISTAKES IN YOUR CSC APPLICATION COULD LEAD TO YOUR APPLICATION BEING REJECTED. Visit http://studyinchina.csc.edu.cn/#/login to CREATE AN ACCOUNT. • The online application works best with Firefox or Internet Explorer (11.0). Menu selection functions may not work with other browsers. • The online application is only available in Chinese and English. 1 • Please read this page carefully before clicking on the “Application online” tab to start your application. 2 • The Program Category is Type B. • The Agency No. matches the university you will be attending. See Appendix A for a list of the Chinese university agency numbers. • Use the + by each section to expand on that section of the form. 3 • Fill out your personal information accurately. o Make sure to have a valid passport at the time of your application. o Use the name and date of birth that are on your passport. Use the name on your passport for all correspondences with the CLIC office or Chinese institutions. o List Canadian as your Nationality, even if you have dual citizenship. Only Canadian citizens are eligible for CLIC support. o Enter the mailing address for where you want your admission documents to be sent under Permanent Address. Leave Current Address blank. Contact your home or host university coordinator to find out when you will receive your admission documents. Contact information for you home university CLIC liaison can be found here: http://clicstudyinchina.com/contact-us/ 4 • Fill out your Education and Employment History accurately. o For Highest Education enter your current degree studies.
    [Show full text]
  • The Fourth International Conference on Law, Language and Discourse (LLD)
    The Fourth International Conference On Law, Language and Discourse (LLD) October 18 -19, 2014, Xi'an, China The American Scholars Press Editors: Le Cheng, Qinglin Ma, Jinbang Du, Lisa Hale, et al. Cover Designer: Jian Li Published by The American Scholars Press, Inc. The Fourth International Conference on Law, Language and Discourse is published by the American Scholars Press, Inc., Marietta, Georgia, USA. No part of this book may be reproduced in any form or by any electronic or mechanical means including information storage and retrieval systems, without permission in writing from the publisher. Copyright © 2014 by the American Scholars Press All rights reserved. ISBN: 978-0-9861817-0-2 Printed in the United States of America 2 Preface Internationally, there is a growing interest from the academia and the legal professionals in the study of the interface between language and law. Locally, Language and Law is one of the core postgraduate programs, which can be traced back to the early 1990s at City University of Hong Kong. In recent years, exchanges and collaborations in language and law among universities such as City University of Hong Kong, Aston University, China University of Political Science and Law, Columbia Law School, Georgetown University and Peking University, have been frequent and productive. With Hong Kong, as an international city, playing an important role of the meeting point for different cultures and with China being the convergence of various jurisdictions, a new association – the Multicultural Association of Law and Language (hereafter as MALL) was founded in 2009 with the Secretariat located in Hong Kong.
    [Show full text]
  • The Joint Academic Program Between Jacksonville State University and Taizhou University: from Inception to Implementation
    The Joint Academic Program Between Jacksonville State University and Taizhou University: From Inception to Implementation Monica TRIFAS and Guillermo FRANCIA, III Department of Mathematical, Computing and Information Sciences Jacksonville State University Jacksonville, AL 36265, USA {atrifas, gfrancia} @jsu.edu Ming YANG School of Computing and Software Engineering Southern Polytechnic State University Marietta, GA 30060 [email protected] ABSTRACT major role in the supply of this resource. Higher education shapes and is being influenced by the configuration of regional trading blocs and is becoming similar across Jacksonville State University, located in Jacksonville, countries [4]. Colleges and universities face the dilemma Alabama, is a state-supported, regional, coeducational of preserving the legitimacy of their national culture when institution. Three years ago, representatives from entering the global market with various beliefs and Jacksonville State University (JSU) began speaking with behaviors [14]. specific staff and faculty members from Taizhou University (TU) located in Linhai, China. Conversations involving possible university collaborations took place Globalization is influencing the educational market and and resulted in a signed memorandum concerning a dual- the situation is changing rapidly in the recent years. The degree computer science program. This paper describes theme of academic globalization is becoming more and some of the evolving collaborative undertakings with more important for many universities around the world, as other Chinese universities as well as the on-going a process that helps the student graduation experience (by experiences and challenges encountered in the creating an expert with new characteristics that cannot be implementation of the dual-degree program with Taizhou provided in their home university, or even in their University.
    [Show full text]
  • 2021 MCM Contest
    2021 Mathematical Contest in Modeling® Press Release—April 23, 2021 COMAP is pleased to announce the results of the 37th annual advantages and disadvantages for fungi species and combinations of Mathematical Contest in Modeling (MCM). This year, 10,053 teams species like to persist is various environments. representing institutions from fifteen countries/regions participated in the contest. Seventeen teams from the following institutions were The B problem used the scenario of the 2019-2020 fire season in designated as OUTSTANDING WINNERS: Australia, which saw devastating wildfires in every state, to consider the use of drones in firefighting. Teams learned of the capabilities of Shanghai Jiao Tong University, China (3) two types of drones, surveillance and situational awareness drones and (2100454 SIAM Award & COMAP Scholarship Award) hovering drones that can carry repeaters (to extend radio range), and Beijing Institute of Technology, China (Ben Fusaro Award) then created a model to determine the optimal numbers and mix of Nanjing University of Posts & Telecommunications, China these two types of drones. Teams addressed adaptation of their model (Frank Giordano Award & SIAM Award) to the changing likelihood of extreme fire events over the next decade, Jiangnan University, China as well as to equipment cost increases. Teams also developed a model Xi'an Jiaotong University, China (AMS Award) to optimize locations of hovering drones for fires of differing sizes on Xidian University, Shannxi, China differing terrain. Beijing Jiaotong University, China (ASA Award) University of Colorado Boulder, CO, USA The C problem investigated the discovery and sightings of Vespa (MAA Award, SIAM Award & COMAP Scholarship Award) mandarina (also known as the Asian giant hornet) in the State of University of Oxford, United Kingdom Washington.
    [Show full text]
  • Astrocytic YAP Promotes the Formation of Glia Scars and Neural Regeneration After Spinal Cord Injury
    2644 • The Journal of Neuroscience, March 25, 2020 • 40(13):2644–2662 Development/Plasticity/Repair Astrocytic YAP Promotes the Formation of Glia Scars and Neural Regeneration after Spinal Cord Injury Changnan Xie,1,2* Xiya Shen,2,3* Xingxing Xu,2* Huitao Liu,1,2 Fayi Li,1,2 Sheng Lu,1,2 Ziran Gao,4 Jingjing Zhang,2 Qian Wu,5 Danlu Yang,2 Xiaomei Bao,2 XFan Zhang,2 Shiyang Wu,1 Zhaoting Lv,5 Minyu Zhu,1 XDingjun Xu,1 Peng Wang,1 Liying Cao,3 Wei Wang,5 Zengqiang Yuan,6 Ying Wang,7 Zhaoyun Li,8 Honglin Teng,1 and Zhihui Huang1,2,3 1Department of Spine Surgery, Wenzhou Medical University First Affiliated Hospital, Wenzhou, Zhejiang 325000, China, 2School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China, 3Key Laboratory of Elemene Anti-cancer Medicine of Zhejiang Province and Holistic Integrative Pharmacy Institutes, Hangzhou Normal University, Hangzhou 311121, China, 4Graduate School of Youjiang Medical University for Nationalities, Basie, Guangxi 533000, China, 5School of Mental Health, Wenzhou Medical University, Zhejiang 325035, China, 6The Brain Science Center, Beijing Institute of Basic Medical Sciences, Beijing 100850, China, 7Department of Transfusion Medicine, Zhejiang Provincial People’s Hospital of Hangzhou Medical College, Hangzhou 310053, China, and 8Department of Clinical Laboratory, Taizhou Central Hospital (Taizhou University Hospital), Taizhou 318000, Zhejiang, China Yes-associated protein (YAP) transcriptional coactivator is negatively regulated by the Hippo pathway and functions in controlling the size of multiple organs, such as liver during development. However, it is not clear whether YAP signaling participates in the process of the formation of glia scars after spinal cord injury (SCI).
    [Show full text]
  • List of Names
    2018 10th International Conference on Intelligent Human-Machine Systems and Cybernetics (IHMSC 2018) Hangzhou, China 25-26 August 2018 Volume 1 Pages 1-392 IEEE Catalog Number: CFP1839H-POD ISBN: 978-1-5386-5837-6 1/2 Copyright © 2018 by the Institute of Electrical and Electronics Engineers, Inc. All Rights Reserved Copyright and Reprint Permissions: Abstracting is permitted with credit to the source. Libraries are permitted to photocopy beyond the limit of U.S. copyright law for private use of patrons those articles in this volume that carry a code at the bottom of the first page, provided the per-copy fee indicated in the code is paid through Copyright Clearance Center, 222 Rosewood Drive, Danvers, MA 01923. For other copying, reprint or republication permission, write to IEEE Copyrights Manager, IEEE Service Center, 445 Hoes Lane, Piscataway, NJ 08854. All rights reserved. *** This is a print representation of what appears in the IEEE Digital Library. Some format issues inherent in the e-media version may also appear in this print version. IEEE Catalog Number: CFP1839H-POD ISBN (Print-On-Demand): 978-1-5386-5837-6 ISBN (Online): 978-1-5386-5836-9 Additional Copies of This Publication Are Available From: Curran Associates, Inc 57 Morehouse Lane Red Hook, NY 12571 USA Phone: (845) 758-0400 Fax: (845) 758-2633 E-mail: [email protected] Web: www.proceedings.com 2018 10th International Conference on Intelligent Human-Machine Systems and Cybernetics IHMSC 2018 Table of Contents Preface xiii Conference Organization xiv Program Committee
    [Show full text]
  • Confidential: for Review Only Clinical Findings of Second-Generation Patients with 2019 Novel Coronavirus: a Multicenter, Retrospective, Observational Study
    BMJ Confidential: For Review Only Clinical findings of second-generation patients with 2019 novel coronavirus: a multicenter, retrospective, observational study Journal: BMJ Manuscript ID BMJ-2020-054741 Article Type: Research BMJ Journal: BMJ Date Submitted by the 31-Jan-2020 Author: Complete List of Authors: Xu, Xiao-Wei ; Zhejiang University First Affiliated Hospital State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Wu, Xiao-Xin ; Zhejiang University First Affiliated Hospital State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Jiang, Xian-Gao; Wenzhou Medical College Ding-li Clinical College Xu, Kai-Jin ; Zhejiang University First Affiliated Hospital State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Ying, Ling-Jun ; Taizhou University Ma, Chun-Lian; First People's Hospital of Wenling Li, Shi-Bo ; Zhejiang University Zhoushan Hospital Wang, Hua-Ying ; Ningbo University Medical School Zhang, Sheng ; Taizhou Hospital of Zhejiang Province Gao, Hainv; Zhejiang Shuren University Sheng, Ji-Fang ; Zhejiang University First Affiliated Hospital State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Cai, Hong-Liu ; Zhejiang University First Affiliated Hospital State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Qiu, Yun-Qing ; Zhejiang University First Affiliated Hospital State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Li, Lanjuan; Zhejiang University First Affiliated Hospital State Key Laboratory for Diagnosis and Treatment
    [Show full text]