DOCSLIB.ORG

(ELISA) to Detect Trace Amounts of Almond (Prunus Dulcis L.) Mengna Su

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
(ELISA) to Detect Trace Amounts of Almond (Prunus Dulcis L.) Mengna Su Florida State University Libraries Electronic Theses, Treatises and Dissertations The Graduate School 2012 Development of a Specific, Sensitive, and Robust Enzyme-Linked Immunosorbent Assay (ELISA) to Detect Trace Amounts of Almond (Prunus Dulcis L.) Mengna Su Follow this and additional works at the FSU Digital Library. For more information, please contact [email protected] THE FLORIDA STATE UNIVERSITY COLLEGE OF HUMAN SCIENCES DEVELOPMENT OF A SPECIFIC, SENSITIVE, AND ROBUST ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT TRACE AMOUNTS OF ALMOND (PRUNUS DULCIS L.) By MENGNA SU A Dissertation submitted to the Department of Nutrition, Food and Exercise Sciences in partial fulfillment of the requirements for the degree of Doctor of Philosophy Degree Awarded: Spring Semester, 2012 Mengna Su defended this dissertation on March 15, 2012. The members of the supervisory committee were: Shridhar K. Sathe Professor Directing Dissertation William T. Cooper III University Representative Yun-Hwa Peggy Hsieh Committee Member Jodee Dorsey Committee Member Mary Ann Moore Committee Member The Graduate School has verified and approved the above-named committee members, and certifies that the dissertation has been approved in accordance with university requirements. ii To my parents, Xingqing Su (苏兴庆) and Qianhua Chen (陈倩华); my husband, Jinqing Xie (谢 锦清); my daughter, Yier Xie (谢漪儿); my brother and sister. iii ACKNOWLEDGEMENTS I believe I will never come to this point without the help of many brilliant and immensely supportive people. Special thanks to my major professor, Dr. Shridhar K. Sathe, for providing the opportunity to work in his lab in NFES at FSU, for suggesting research direction, for instructing in person of key lab skills, for the excellent and quality scientific instruments and materials made available, for the freedom in pursuing my research interest, and for the tough four years and eight months training…I would like to deeply thank Dr. Yun-Hwa Peggy Hsieh for serving in my committee, providing me guidance, and teaching me basics of immunoassay. I also thank Dr. Mary Ann Moore, Dr. Jodee Dorsey and Dr. William T. Cooper for serving in my committee, providing valuable inputs, and supporting me throughout this program. Thanks to Drs. Bahram Arjmandi, Yun-Hwa Peggy Hsieh and Jeong-Su Kim for allowing me use their lab instruments to assist my research. Thanks to Ms. Margaret Seavy and Dr. Giordano Fiore at the analytical facilities of Dept. of Biological Science for their help in protein N-terminal sequencing. I sincerely thank College of Human Sciences and the Department of Nutrition, Food, and Exercise Sciences for providing the opportunity and financial support to pursue my doctoral degree. Funds from the USDA-AFRI (2009-65503-05797 & 2011-67017-20079), Washington, DC; Almond Board of California, Modesto, CA; the Florida State University Research Fundation Grant Assistance Program (GAP) grant, Tallahassee, FL; the Florida State University Dissertation Research Grant (DRG), Tallahassee, FL; and College of Human Sciences Dissertation Award Program (DAP), Tallahassee, FL are greatly appreciated. Many thanks to Dr. Mary Ann Moore, Dr. Bahram Arjmandi, Ms. Marian Sumner, Ms. Regina Fassig and the administrative personnel, Ms. Tara Hartman, Ms. Ann Smith, Mr. David Parish, Mr. Joseph Carroll, Ms. Barbara Koehler, Ms. Jeannine Spears, Mr.Andy Zimmerman, and Mr.General Gaskin for always being there to help!! My labmates, Mahesh, Harshal, Girdhari, Mimi, Shyamali, Ying, Changqi and Sahil; and LeAnna from Dr. Roux’s lab are greatly thanked for their research support and making work in the lab very joyful. Special thanks to Shyamali, Haiyan, Changqi, Ying, and Sophie for their great help in the preparation of dissertation and defense! I am deeply thankful to Pei-yang, iv Sheauching, Haiyan, and Bi-jen for accompanying me through this period and helping me out always!! I enjoy friendship from Qinchun, Anuri, Yi-tien, Michael, Kamil, Jack, Florence, Dr. Shim, Nancy, Quanhong, Sophie, Shirin, Raz, Abbey, Jiyao, Marcus, Rafaela, Zahra, Liza, Jenna, Ajay, Alexei, and Monica. My friends, Haihong, Jingfang, Xu hui and their families, and Xiaoli, thank you for all the happiness you brought to me! Finally, all my endeavors are dedicated to my parents, my daughter, and my husband for their constant and endless love, encouragement and support. Special sorry goes to Yier for the invisible mom whom is always “in” the window of “google talk” or at the other end of telephone line… v TABLE OF CONTENTS List of Tables ...................................................................................................................................x List of Figures ............................................................................................................................... xii List of Terms and Abbreviations ................................................................................................ xiv Abstract ...................................................................................................................................... xviii 1. INTRODUCTION ...................................................................................................................1 1.1 Background and Significance ........................................................................................1 1.1.1 Almond ..............................................................................................................1 1.1.2 Allergy and Tree Nut Allergy ............................................................................3 1.1.3 Almond Allergens and Almond Allergy ............................................................6 1.1.4 Need for Food Allergen Detection Methods ....................................................10 1.1.5 Available Almond Detection Methods ............................................................13 1.2 Statement of Problem ...................................................................................................17 1.3 Central Hypothesis .......................................................................................................18 1.4 Specific Aims ...............................................................................................................18 2. PURIFICATON AND CHARACTERIZATION OF ANTI-ALMOND SOLUBLE PROTEIN MURINE MONOCLONAL ANTIBODIES ...............................................................20 2.1 Materials and Methods .................................................................................................22 2.1.1 Materials ..........................................................................................................22 2.1.2 Methods............................................................................................................24 2.1.2.1 Antibody Production and Screening of mAbs ...................................24 2.1.2.2 Preparation of Protein Extracts ..........................................................24 2.1.2.3 Protein Determination ........................................................................25 2.1.2.4 Antibody Purification.........................................................................25 2.1.2.5 SDS PAGE with CBBR Staining .......................................................26 2.1.2.6 Silver Staining ....................................................................................26 2.1.2.7 Standardization of Purified mAb .......................................................27 2.2.2.7.1 IgG Concentration .............................................................27 2.2.2.7.2 Ab Titer .............................................................................27 2.2.2.7.3 Ag Titer .............................................................................27 2.1.2.8 Epitope Comparison and Antigen Identification ...............................27 2.2.2.8.1 Indirect ELISA ..................................................................27 2.2.2.8.2 ELISA Additivity Test ......................................................28 2.2.2.8.3 Column Chromatography..................................................29 2.2.2.8.4 Non-denaturing Non-dissociating-Polyacrylamide Gel Electrophoresis (NDND PAGE) .......................................................29 2.2.2.8.5 Western Blotting ...............................................................30 2.2.2.8.6 Molecular Weight (MW) Determination by SDS PAGE .30 2.2.2.8.7 Cyanogen Bromide (CNBr) Affinity Chromatography ....31 2.2.2.8.8 N-terminal Amino Acid Sequencing ................................31 2.1.2.9 Statistical Analysis .............................................................................32 2.2 Results and Discussion ................................................................................................32 2.2.1 Antibody Production and Screening of mAbs .................................................32 2.2.2 Antibody Purification.......................................................................................32 2.2.3 Standardization of Purified mAbs ....................................................................34 vi 2.2.4 Epitope Comparison and Antigen Identification .............................................40 2.3.4.1 ELISA additivity test .........................................................................40 2.3.4.2 Column Chromatography...................................................................40 2.3.4.3 Molecular Weight (MW) Determination by SDS PAGE ..................48
Load more

Recommended publications
  • Pharmacodynamic Considerations in the Critically Ill Antibiotic Pharmacokinetic/Pharmacodynamic Considerations in the Critically Ill
    Andrew A. Udy Jason A. Roberts Je rey Lipman Editors Antibiotic Pharmacokinetic/ Pharmacodynamic Considerations in the Critically Ill Antibiotic Pharmacokinetic/Pharmacodynamic Considerations in the Critically Ill [email protected] Andrew A. Udy • Jason A. Roberts • Jeffrey Lipman Editors Antibiotic Pharmacokinetic/ Pharmacodynamic Considerations in the Critically Ill [email protected] Editors Andrew A. Udy Jason A. Roberts Australian and New Zealand Intensive Care Burns, Trauma and Critical Care Research Centre Research Centre School of Public Health and Preventive The University of Queensland Medicine, Monash University UQ Centre for Clinical Research Melbourne Herston Victoria Queensland Australia Australia Jeffrey Lipman Burns, Trauma and Critical Care Research Centre The University of Queensland Herston Queensland Australia ISBN 978-981-10-5335-1 ISBN 978-981-10-5336-8 (eBook) DOI 10.1007/978-981-10-5336-8 Library of Congress Control Number: 2017952539 © Springer Nature Singapore Pte Ltd. 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication.
    [Show full text]
  • The History of Painting in Italy, Vol. V
    The History Of Painting In Italy, Vol. V By Luigi Antonio Lanzi HISTORY OF PAINTING IN UPPER ITALY. BOOK III. BOLOGNESE SCHOOL. During the progress of the present work, it has been observed that the fame of the art, in common with that of letters and of arms, has been transferred from place to place; and that wherever it fixed its seat, its influence tended to the perfection of some branch of painting, which by preceding artists had been less studied, or less understood. Towards the close of the sixteenth century, indeed, there seemed not to be left in nature, any kind of beauty, in its outward forms or aspect, that had not been admired and represented by some great master; insomuch that the artist, however ambitious, was compelled, as an imitator of nature, to become, likewise, an imitator of the best masters; while the discovery of new styles depended upon a more or less skilful combination of the old. Thus the sole career that remained open for the display of human genius was that of imitation; as it appeared impossible to design figures more masterly than those of Bonarruoti or Da Vinci, to express them with more grace than Raffaello, with more animated colours than those of Titian, with more lively motions than those of Tintoretto, or to give them a richer drapery and ornaments than Paul Veronese; to present them to the eye at every degree of distance, and in perspective, with more art, more fulness, and more enchanting power than fell to the genius of Coreggio. Accordingly the path of imitation was at that time pursued by every school, though with very little method.
    [Show full text]
  • Aes Corporation
    THE AES CORPORATION THE AES CORPORATION The global power company A Passion to Serve A Passion A PASSION to SERVE 2000 ANNUAL REPORT ANNUAL REPORT THE AES CORPORATION 1001 North 19th Street 2000 Arlington, Virginia 22209 USA (703) 522-1315 CONTENTS OFFICES 1 AES at a Glance AES CORPORATION AES HORIZONS THINK AES (CORPORATE OFFICE) Richmond, United Kingdom Arlington, Virginia 2 Note from the Chairman 1001 North 19th Street AES OASIS AES TRANSPOWER Arlington, Virginia 22209 Suite 802, 8th Floor #16-05 Six Battery Road 5 Our Annual Letter USA City Tower 2 049909 Singapore Phone: (703) 522-1315 Sheikh Zayed Road Phone: 65-533-0515 17 AES Worldwide Overview Fax: (703) 528-4510 P.O. Box 62843 Fax: 65-535-7287 AES AMERICAS Dubai, United Arab Emirates 33 AES People Arlington, Virginia Phone: 97-14-332-9699 REGISTRAR AND Fax: 97-14-332-6787 TRANSFER AGENT: 83 2000 AES Financial Review AES ANDES FIRST CHICAGO TRUST AES ORIENT Avenida del Libertador COMPANY OF NEW YORK, 26/F. Entertainment Building 602 13th Floor A DIVISION OF EQUISERVE 30 Queen’s Road Central 1001 Capital Federal P.O. Box 2500 Hong Kong Buenos Aires, Argentina Jersey City, New Jersey 07303 Phone: 852-2842-5111 Phone: 54-11-4816-1502 USA Fax: 852-2530-1673 Fax: 54-11-4816-6605 Shareholder Relations AES AURORA AES PACIFIC Phone: (800) 519-3111 100 Pine Street Arlington, Virginia STOCK LISTING: Suite 3300 NYSE Symbol: AES AES ENTERPRISE San Francisco, California 94111 Investor Relations Contact: Arlington, Virginia USA $217 $31 Kenneth R. Woodcock 93% 92% AES ELECTRIC Phone: (415) 395-7899 $1.46* 91% Senior Vice President 89% Burleigh House Fax: (415) 395-7891 88% 1001 North 19th Street $.96* 18 Parkshot $.84* AES SÃO PAULO Arlington, Virginia 22209 Richmond TW9 2RG $21 Av.
    [Show full text]
  • Transcriptional and Post-Transcriptional Profile of Human Chromosome 21
    Downloaded from genome.cshlp.org on October 6, 2021 - Published by Cold Spring Harbor Laboratory Press Methods Transcriptional and post-transcriptional profile of human chromosome 21 Sergey I. Nikolaev,1,5 Samuel Deutsch,1,5 Raphael Genolet,2 Christelle Borel,1 Leila Parand,1 Catherine Ucla,1 Frederic Schu¨tz,3 Genevieve Duriaux Sail,1 Yann Dupre´,1 Pascale Jaquier-Gubler,2 Tanguy Araud,2 Beatrice Conne,1 Patrick Descombes,4 Jean-Dominique Vassalli,1 Joseph Curran,2 and Stylianos E. Antonarakis1,6 1Department of Genetic Medicine and Development, University of Geneva Medical School, CH-1211 Geneva, Switzerland; 2Department of Microbiology and Molecular Medicine, University of Geneva Medical School, CH-1211 Geneva, Switzerland; 3Swiss Bioinformatics Institute, Genopode Builiding, CH-1015 Lausanne, Switzerland; 4Genomics Platform, University of Geneva Medical School, CH-1211 Geneva, Switzerland Recent studies have demonstrated extensive transcriptional activity across the human genome, a substantial fraction of which is not associated with any functional annotation. However, very little is known regarding the post-transcriptional processes that operate within the different classes of RNA molecules. To characterize the post-transcriptional properties of expressed sequences from human chromosome 21 (HSA21), we separated RNA molecules from three cell lines (GM06990, HeLa S3, and SK-N-AS) according to their ribosome content by sucrose gradient fractionation. Poly- ribosomal-associated RNA and total RNA were subsequently hybridized to genomic tiling arrays. We found that ;50% of the transcriptional signals were located outside of annotated exons and were considered as TARs (transcriptionally active regions). Although TARs were observed among polysome-associated RNAs, RT-PCR and RACE experiments revealed that ;40% were likely to represent nonspecific cross-hybridization artifacts.
    [Show full text]
  • Nostri Autori Biblioteche Libraries Bibliotheken
    1896 - 2021 125 ANNI YEARS JAHRE JAHRE | YEARS | ANNI 125 NOSTRI OUR UNSERE AUTORI AUTHORS AUTOREN monografie monographs Monografien e riviste and journal und Zeitschriften L’ERMA DI BRETSCHNEIDER 125 ANNI ISBN 978-88-913-2242-5 BIBLIOTECHE LIBRARIES www.lerma.it «L’ERMA» BIBLIOTHEKEN Copertina 125 Dario 7 mm.indd 1 05/07/21 17:35 125 ANNI DELLA CASA EDITRICE 125 JAHRE VERLAG 125 YEARS OF THE PUBLISHING HOUSE 1896-2021 I NOSTRI AUTORI - UNSERE AUTOREN OUR AUTHORS BIBLIOTECHE - BIBLIOTHEKEN LIBRARIES 1 JUNE 1896 • 125 YEARS • 1 JUNE 2021 Roma - Bristol L’ERMA di BRETSCHNEIDER 125 ANNI DELLA CASA EDITRICE 125 JAHRE VERLAG 125 YEARS OF THE PUBLISHING HOUSE 1896-2021 Catalogo a cura di Roberto Marcucci Ricerca e catalogazione David Chacon Giovanni Ligabue Sandro Mannelli Luigi Filippo Mariani Elaborazione informatica e Impaginazione Dario Scianetti Copertina Maurizio Pinto «L’ERMA» di BRETSCHNEIDER • STAFF Elena Montani Maurizio Pinto Dario Scianetti Rossella Corcione Alessio Gasparri Alessia Francescangeli Elena Fontanella David Chacon Giovanni Ligabue Francesco Cagliuso Sandro Mannelli Luigi Filippo Mariani I frontespizi dei volumi antichi presenti in questo catalogo sono stati recuperati sul mercato antiquario. Nel corso degli anni i vari eventi e soprattutto le due guerre, hanno reso difficoltoso rintracciare i volumi più antichi. The title pages of the old books reproduced in this catalogue, were recovered from the antiquarian market. The march of time, and above all the events of two World Wars, have made very difficult to track down the older books published by Max Bretschneider. Die in dieser Broschüre abgebildeten Titelseiten der alten Bücher sind in Antiquariaten gefunden worden.
    [Show full text]
  • XLI Mostra Internazionale Del Cinema Libero Fondazione Cineteca Di Bologna
    XLI Mostra Internazionale del Cinema Libero Fondazione Cineteca di Bologna “Il cinema è l’istituzione più democratica e più internazionale... Apre spazio alla fantasia creativa degli spettatori [...] costretti e autorizzati a dare loro stessi un’anima a quei corpi in movimento; detto altrimenti: a scriversi il proprio testo per il film” “Cinema is the most democratic and most international of institutions... Provides space for the viewer’s creative imagination [...] compelled and enabled to give a soul to those moving bodies – or, to put it more simply, to write their own text for the pictures” Viktor Klemperer, 1912 “Procediamo con disordine. Il disordine dà qualche speranza, l’ordine nessuna” “Let’s start with disorder. Disorder gives you some hope, order none” Marcello Marchesi, 1963 Promosso da / Promoted by: FONDAZIONE CINETECA DI BOLOGNA Coordinator: Sara Mastrodomenico Fondazione Cineteca di Bologna Presidente / President: Carlo Mazzacurati Coordinamento Cinema Lumière 1 / Cinema Ente Mostra Internazionale del Cinema Libero Direttore / Director: Gian Luca Farinelli Lumière 1 coordinator: Andrea Peraro Consiglio di amministrazione / Board of Coordinamento Cinema Lumière 2 / Cinema Con il sostegno di / With the support of: Directors: Carlo Mazzacurati (Presidente), Lumière 2 coordinator: Nicola Di Battista Comune di Bologna Alina Marazzi, Valerio De Paolis Coordinamento Piazza Maggiore / Piazza Ministero per i Beni e le Attività Culturali - Maggiore coordinator: Silvia Fessia con Direzione Generale per il Cinema Ente Mostra Internazionale
    [Show full text]
  • Catalogo Ufficiale Della Manifestazione (Con Risultati)
    48° Esposizione canina Internazionale Gonzaga (MN) 05-06/03/2011 Catalogo ufficiale della manifestazione (con risultati) I dati contenuti sono da ritenersi non definitivi ai sensi dell'art. 13 del Regolamento generale delle manifestazioni canine. I Risultati sono stati trascritti dalle relazioni di giudizio salvo errori e/o omissioni di cui non si assume alcuna responsabilità Delegazione SIS Mantova e Gruppo Cinofilo Virgiliano Via P. Verri, 33 46100 Mantova www.gruppocinofilovirgiliano.it Pagina 1 di 137 Gruppo Fci: 1 CANI DA PASTORE E BOVARI Sezione Fci: 1a - Cani da pastore 15-CANE DA PASTORE BELGA GROENENDAEL Nazionalità: B LIBERA - Maschi 1 - ADONE Cl. 2 Nato 14/02/2008 - nero - LO0924560 - Tat. 982009105766039 Ql. ECC da Ghibli Di Casa Bellegarde e da Forever Di Casa Caniglia All.re Marcoz Paolo Propr. Pedrani Rosa Anna INTERMEDIA - Maschi 2 - FLYS -G DEL FESTOSO Cl. 1 Nato 20/06/2009 - nero - LO09160021 - Tat. 380260040220007 Ql. ECC da Ch: Luavjon' S Broadway -g e da Ch. Haidy -g Del Festoso All.re Guin Renato Propr. Stefani Maurizio GIOVANI - Maschi 3 - FIGARO' DES LOUPS DE MOUSETTE Cl. Nato 15/03/2010 - nero - LO1086225 - Tat. 380260000000927 Ql. da S.r.pe Verlain Dell'alta Via e da La Rosa Tatuata Callas All.re Adorni Massimo Propr. All. Loups De Mousette LIBERA - Femmine 4 - DESY- DEL FESTOSO Cl. 1 Nata 20/04/2008 - nero - LO0941602 - Tat. 982009106405493 Ql. ECC CAC CIB da Ch. Tuono-g Del Festoso e da Ch. Haidy-g Del Festoso All.re Guin Renato Propr. Stefani Maurizio GIOVANI - Femmine 5 - MELODY-G Cl. 1 Nata 28/04/2010 - nero - LO10193263 - Tat.
    [Show full text]
  • Notiziario Bibliografico, Parte II: Materie E Discipline CLXVI
    Notiziario bibliografico, parte II: Materie e discipline CLXVI NOTIZIARIO BIBLIOGRAFICO Parte II: Materie e discipline (2016/2018) a cura di Giovanni Cupaiuolo I titoli dei periodici sono di norma indicati con le sigle in uso nell’ Année philologique: AAntHung = Acta antiqua Academiae Scientiarum Hungaricae ACD = Acta Classica Universitatis Scientiarum Debreceniensis AAP = Atti Accademia Pontaniana AAT = Atti Accademia delle Scienze di Torino A&A = Antike und Abendland A&C = Actas y Comunicaciones del Instituto de Historia Antigua y Medieval A&R = Atene e Roma Aevum(ant) = Aevum Antiquum AFilosC = Anais de Filosofia Clássica AgCent = Agri centuriati AION(filol) = Annali dell'Università degli Studi di Napoli "L'Orientale", Sez. di Filologia e Letteratura AJ = Archai Journal AJPh = American Journal of Philology ALRiv = Rivista di studi di Anthologia Latina ALMA = Archivum Latinitatis Medii Aevi AncNarr = Ancient Narrative AnnSE = Annali di storia dell’esegesi AntPhilos = Antiquorum philosophia AntTard = Antiquité Tardive AOFL = Annali Online di Ferrara-Lettere APapyrol = Analecta Papyrologica ARF = Appunti romani di filologia ARID = Analecta Romana Instituti Danici AS = Ancient Society ASNP = Annali della Scuola Normale Speriore di Pisa ASUS = Annali di studi umanistici di Siena BC = Bollettino dei classici BEC = Boletim de Estudos Classicos BICS = Bulletin of the Institute of Classical Studies BIDR = Bullettino dell’Istituto di diritto romano ‘Vittorio Scialoja' BMCRev = Bryn Mawr Classical Review BStudLat = Bollettino di studi latini
    [Show full text]
  • Agenda Paolina
    2018 AGENDA PAOLINA con riflessioni quotidiane dagli scritti del beato Giacomo Alberione Anno LXII © SASP s.r.l., 2017 Copertina di Ulysses Navarro, ssp La Fede, mosaico deambulatorio. Roma, Cripta Santuario Santa Maria Regina degli Apostoli. PRESENTAZIONE «Persone che si nutrono del Vangelo». Queste pa- role del Beato Giacomo Alberione ci ricordano tutto il suo amore alla Parola di Dio e la necessità di nu- trirsi di essa. “Mangiare” la Parola è infatti indi- spensabile per la nostra vita apostolica nella cultura della comunicazione moderna. Assimilare il Vangelo equivale a nutrire la mente di Gesù Maestro, ruota del Carro paolino necessaria, tutta orientata all’e- vangelizzazione. I pensieri di questa Agenda Paolina 2018 ci aiutano a ripercorrere parte dei molti inter- venti del nostro Fondatore sul valore dello studio – o meglio della studiosità – di quella dimensione, cioè, della vita paolina che ci permette di essere aggiorna- ti e di “ringiovanire” la mente, processo fondamen- tale per raggiungere l’umanità di oggi. Questa prospettiva dello studio ci ha ispirato a selezionare alcuni brani in sintonia con il prossimo Sinodo dei vescovi sui giovani, che sarà celebrato nel mese di ottobre a Roma. I giovani, la fede e il di- scernimento vocazionale: ecco il percorso che aiuta anche noi a ravvivare il dono della vocazione e ad essere uomini e donne che generano, che testimo- niano alle nuove generazioni il cuore della vita e missione della Famiglia Paolina. Qua e là incontre- remo nell’Agenda brani tratti dal Diario del Beato Timoteo Giaccardo, per dar voce a quella vivacità tipica dei primi tempi, dove iniziavano a giungere nella prima Casa paolina giovani che avevano cono- sciuto Don Alberione e la sua opera.
    [Show full text]
  • Natura Giuridica
    Anno 2011 - STRUTTURA WELFARE LOCALE NATURA GIURIDICA COGNOME NOME DENOMINAZIONE (persona fisica o ESTREMI DELLA NORMA IN FORZA DELLA ESTREMI E TIPO DI ATTO RAGIONE SOCIALE Data di nascita giuridica) QUALE E' CORRISPOSTO IL BENEFICIO AUTORIZZATORIO DELIBERA CONSIGLIO UNIONE N. 26 DEL ABASLAMI NOUREDDINE 13/9/1983 PERSONA FISICA 12/07/2005 D.D.235 DEL 28/10/2011 DELIBERA CONSIGLIO UNIONE N. 26 DEL D.D.130 DEL 30/07/2010, D.D.175 DEL Abate Michelina 20/9/1967 PERSONA FISICA 12/07/2005 31/08/2011 DELIBERA CONSIGLIO UNIONE N. 26 DEL Abdssamad Omar 16/8/1978 PERSONA FISICA 12/07/2005 D.D.210 DEL 30/09/2011 DELIBERA CONSIGLIO UNIONE N. 26 DEL Abel Adelaide Esi Enyiniwah 8/6/1982 PERSONA FISICA 12/07/2005 D.D.265 DEL 30/11/2011 DELIBERA CONSIGLIO UNIONE N. 26 DEL Aberathna Herath Aberathna Menike 8/3/1967 PERSONA FISICA 12/07/2005 D.D.268 DEL 31/12/2010 DELIBERA CONSIGLIO UNIONE N. 26 DEL ABID BOUCHRA 5/6/1974 PERSONA FISICA 12/07/2005 D.D.193 DEL 29/10/2010 DELIBERA CONSIGLIO UNIONE N. 26 DEL Abida Said 1/1/1968 PERSONA FISICA 12/07/2005 D.D.139 DEL 30/06/2011 DELIBERA CONSIGLIO UNIONE N. 26 DEL D.D.117 DEL 30/06/2010, D.D.175 DEL ABIODUN ASORO SARAH 23/3/1977 PERSONA FISICA 12/07/2005 31/08/2011 DELIBERA CONSIGLIO UNIONE N. 26 DEL D.D.117 DEL 30/06/2010, D.D.157 DEL ABKHAR BRAHIM 25/12/1956 PERSONA FISICA 12/07/2005 29/07/2011, D.D.265 DEL 30/11/2011 DELIBERA CONSIGLIO UNIONE N.
    [Show full text]
  • Andrew, Asbury-Bagnati
    THE AGES DIGITAL LIBRARY REFERENCE CYCLOPEDIA of BIBLICAL, THEOLOGICAL and ECCLESIASTICAL LITERATURE Andrew, Asbury - Bagnati, Simeone by James Strong & John McClintock To the Students of the Words, Works and Ways of God: Welcome to the AGES Digital Library. We trust your experience with this and other volumes in the Library fulfills our motto and vision which is our commitment to you: MAKING THE WORDS OF THE WISE AVAILABLE TO ALL — INEXPENSIVELY. AGES Software Rio, WI USA Version 1.0 © 2000 2 Andrew, Asbury, a Methodist Episcopal minister, was born in Caroline County, Md., in 1825. He removed to the vicinity of Fort Wayne, Ind., in 1833; experienced conversion in 1840; and in 1845 entered the Indiana Conference, in which he filled acceptably the appointments assigned him until his superannuation, which took place six years previous to his death. He died July 19, 1870. Mr. Andrew was a man of deep piety and respectable preaching ability. See Minutes of Annual Conf., 1871, p. 114. Andrew, James Osgood, D.D., a bishop of the Methodist Episcopal Church, South, was born in Wilkes County, Ga., May 3, 1794. His father, the Rev. John Andrew, was one of the early itinerant Methodist preachers. His mother’s maiden name was Crosby. She was possessed of a strong intellect, fine taste, and deep piety elements that strongly marked the bishop’s character. He was an extensive reader from his childhood, joined the Church at the age of thirteen, soon became class-leader, and when eighteen was licensed to preach. His first pulpit efforts were among the negroes, and were crowned with success.
    [Show full text]
  • Acknowledgement to Reviewers of Applied Sciences in 2018 Applied Sciences Editorial Office
    Editorial Acknowledgement to Reviewers of Applied Sciences in 2018 Applied Sciences Editorial Office MDPI, St. Alban-Anlage 66, 4052 Basel, Switzerland Published: 8 January 2019 Rigorous peer-review is the corner-stone of high-quality academic publishing. The editorial team greatly appreciates the reviewers who contributed their knowledge and expertise to the journal’s editorial process over the past 12 months. In 2018, a total of 2759 papers were published in the journal, with a median time to first decision of 15 days and a median time to publication of 36 days. The editors would like to express their sincere gratitude to the following reviewers for their cooperation and dedication in 2018: A. Barbier Adam Glowacz Agata Jakóbik-Kolon A. Burl Donaldson Adam Gonzalez Aggelos S. Bouhouras A. Rajesh Adam Grajcar Aggrey Mwesigye A. S. Guimaraes Adam Kawalec Agnieszka Landowska A. S. M. Sanwar Hosen Adam Leadbetter Agnieszka Pręgowska A. Topa Gomes Adam Martowicz Agnieszka Woszuk A.G. D’Aloia Adam Mock Agnieszka Zalejska-Jonsson A.J. Yuste-Delgado Adam Moroz Aguinaldo Fraddosio Aarti Jajoo Adam S Chatterley Agustin Valera Medina Aashwin Mishra Adam Santorelli Ahlem Arfaoui Abbas Barfidokht Adam Schmidt Ah-Lian Kor Abbas Madani Adam Sedziwy̧ Ahmad Alhasan Abd Rashid Bin Mohd Yusoff Adam Szeląg Ahmad Almadhor Abdelkader Kahouli Adam Walker Ahmad Barari Abdelkrim Brahmi Adam Weiss Ahmad Jalal Abdelmadjid Boudjemai Adam Wisniewski Ahmad Karnama Abdelrahman Zkria Adam Wittek Ahmad Mostafa Abdel-Shakour Abuzneid Addisson Salazar Ahmad Salaimeh Abdenour Hadid Adedotun J. Agbemuko Ahmadreza Baghaie Abderrahim Lakhouit Adel Alhalawani Ahmed A. El-Gendy Abdollah Dehzangi Adel El-Shahat Ahmed Abdalrahman Abdollah Saboori Adelaide Miranda Ahmed Abdel-Mohti Abdolrahman Peimankar Adele Fusco Ahmed Abu-Siada Abdul Halim Miah Adelino J.
    [Show full text]